期刊
CHEMICAL SCIENCE
卷 4, 期 12, 页码 4512-4519出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3sc51725j
关键词
-
资金
- EPSRC/Wellcome Trust Fellowships
- Yorkshire Cancer Research
- Wellcome Trust [077544]
The characterization and bioactivity of the dinuclear ruthenium(II) complex [(Ru(DIP) 2) 2(tpphz)] 4+ (DIP 4,7-diphenyl-1,10-phenanthroline and tpphz tetrapyrido[3,2-a: 2',3'-c: 3 '',2 ''-h: 2 ''',3 '''-j] phenazine) is reported. This new complex is found to be luminescent in acetonitrile, where excitation into MLCT (metal-to-ligand charge-transfer) bands in the visible area of the spectrum (lex 450 nm, 3 45 000 M-1 cm(-1)) result in red emission (lem, max 620 nm, FMLCT 0.017). Aqueous in vitro binding studies indicate that this complex binds to duplex DNA with an affinity of 1.8 x 106 M-1 through a nonclassical groove-binding interaction, however, unlike the parent complex [(Ru(phen) 2) 2(tpphz)] 4+ (phen 1,10-phenanthroline), it also displays an increase in MLCT luminescence on addition of liposomes. Confocal microscopy and TEM studies show that this lipophilic complex targets the endoplasmic reticulum of eukaryotic cells, where it functions as an imaging agent for this organelle, and cytotoxicity studies in human cancer cell lines indicate a comparable potency to the anti-cancer drug cisplatin.
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