期刊
JOURNAL OF BREATH RESEARCH
卷 8, 期 1, 页码 -出版社
IOP PUBLISHING LTD
DOI: 10.1088/1752-7155/8/1/016005
关键词
residual gas analyzer; Helicobacter pylori; breath analysis; off-axis integrated cavity output spectroscopy
资金
- S N Bose Centre [SNB/MP/11-12/69]
- UKIERI project grant [IND/CONT/E/11-12/086]
- Thematic Unit [DST-SR/NM/NS-09/2011]
- DST Inspire Fellowships
- JRF studentships from the S N Bose Centre
- DBT for the award of Rapid Grant for Young Investigators (RGYI)
A residual gas analyzer (RGA) coupled with a high vacuum chamber is described for the non-invasive diagnosis of the Helicobacter pylori (H. pylori) infection through C-13-urea breath analysis. The present RGA-based mass spectrometry (MS) method is capable of measuring high-precision (CO2)-C-13 isotope enrichments in exhaled breath samples from individuals harboring the H. pylori infection. The system exhibited 100% diagnostic sensitivity, and 93% specificity alongside positive and negative predictive values of 95% and 100%, respectively, compared with invasive endoscopy-based biopsy tests. A statistically sound diagnostic cut-off value for the presence of H. pylori was determined to be 3.0% using a receiver operating characteristic curve analysis. The diagnostic accuracy and validity of the results are also supported by optical off-axis integrated cavity output spectroscopy measurements. The delta(13) C-DOB% values of both methods correlated well (R-2 = 0.9973 at 30 min). The RGA-based instrumental setup described here is simple, robust, easy-to-use and more portable and cost-effective compared to all other currently available detection methods, thus making it a new point-of-care medical diagnostic tool for the purpose of large-scale screening of the H. pylori infection in real time. The RGA-MS technique should have broad applicability for C-13-breath tests in a wide range of biomedical research and clinical diagnostics for many other diseases and metabolic disorders.
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