4.4 Article

A multi-object spectral imaging instrument

期刊

JOURNAL OF OPTICS
卷 15, 期 8, 页码 -

出版社

IOP PUBLISHING LTD
DOI: 10.1088/2040-8978/15/8/085302

关键词

spectral imaging; multi-object spectrometer; digital micromirror device

类别

资金

  1. Royal Society
  2. EPSRC
  3. EPSRC [EP/H008799/2, EP/H007636/1, EP/H008799/1] Funding Source: UKRI
  4. Engineering and Physical Sciences Research Council [EP/H008799/2, EP/H008799/1, EP/H007636/1] Funding Source: researchfish

向作者/读者索取更多资源

We have developed a snapshot spectral imaging system which fits onto the side camera port of a commercial inverted microscope. The system provides spectra, in real time, from multiple points randomly selected on the microscope image. Light from the selected points in the sample is directed from the side port imaging arm using a digital micromirror device to a spectrometer arm based on a dispersing prism and CCD camera. A multi-line laser source is used to calibrate the pixel positions on the CCD for wavelength. A CMOS camera on the front port of the microscope allows the full image of the sample to be displayed and can also be used for particle tracking, providing spectra of multiple particles moving in the sample. We demonstrate the system by recording the spectra of multiple fluorescent beads in aqueous solution and from multiple points along a microscope sample channel containing a mixture of red and blue dye.

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