Article
Optics
Tristan C. Paul, Guy Hagen
Summary: A new technique called Spinning Disk Structured Illumination Microscopy (SD-SIM) was investigated, which combines Spinning Disk microscopy with Structured Illumination Microscopy to achieve better background removal and detect cellular structures that were previously undetectable with either method alone.
Article
Multidisciplinary Sciences
Xianglei Liu, Artiom Skripka, Yingming Lai, Cheng Jiang, Jingdan Liu, Fiorenzo Vetrone, Jinyang Liang
Summary: The article introduces a method for wide-field video-rate upconversion temperature sensing using compressive sensing technology, which enables video temperature measurement on dynamic samples.
NATURE COMMUNICATIONS
(2021)
Article
Physics, Applied
Mark Strassberg, Yana Shevtsova, Domenick Kamel, Kai Wagoner-Oshima, Hualin Zhong, Min Xu
Summary: PDIC technique utilizes polarization camera to record unfiltered Stokes vector of the differential interference pattern, achieving high spatial resolution phase imaging. By introducing total variation regularization algorithm to solve quantitative phase map from partial derivative along a single axis, accuracy and image quality are improved compared to Fourier transform solution.
APPLIED PHYSICS LETTERS
(2021)
Article
Chemistry, Physical
Cecilia Zaza, German Chiarelli, Ludovit P. Zweifel, Mauricio Pilo-Pais, Evangelos Sisamakis, Fabio Barachati, Fernando D. Stefani, Guillermo P. Acuna
Summary: Fluorescence Resonance Energy Transfer (FRET)-based approaches are unique tools for sensing the immediate surroundings and interactions of (bio)molecules. FRET imaging and Fluorescence Lifetime Imaging Microscopy (FLIM) enable the visualization of the spatial distribution of molecular interactions and functional states. However, conventional FLIM and FRET imaging provide average information over an ensemble of molecules within a diffraction-limited volume, which limits the spatial information, accuracy, and dynamic range of the observed signals. Here, an approach to obtain super-resolved FRET imaging based on single-molecule localization microscopy using an early prototype of a commercial time-resolved confocal microscope is demonstrated. DNA Points Accumulation for Imaging in Nanoscale Topography with fluorogenic probes provides a suitable combination of background reduction and binding kinetics compatible with the scanning speed of usual confocal microscopes. A single laser is used to excite the donor, a broad detection band is employed to retrieve both donor and acceptor emission, and FRET events are detected from lifetime information.
Article
Chemistry, Analytical
Ruixi Chen, Hongfu Zhou, Anan Li, Xiaofeng Cheng, Xiaoxiang Liu, Fei Huang, Yu Wang, Yurong Liu, Hui Gong, Xiuli Liu, Shaoqun Zeng
Summary: Immunofluorescence chemical sectioning (IF-CS) utilizes complexation reactions to provide an off/on mechanism for Alexa dyes, allowing CS imaging of IF labeled tissues, which solves the conflict in traditional imaging methods. IF-CS demonstrates its utility in wide-field imaging of large immunolabeled tissues with three-dimensional submicron resolution, greatly facilitating systematic studies of refined subcellular architectures in intact biological systems.
ANALYTICAL CHEMISTRY
(2021)
Article
Nanoscience & Nanotechnology
Mohit Rathor, Shivam Kumar Chaubey, Rupen Tamang, Biplob Koch, Rakesh Kumar Singh
Summary: This paper presents a label-free and single-shot noninvasive quantitative anisotropy imaging method for cancer cells using a polarization digital holographic microscope (PDHM). The PDHM's ability to tune the carrier frequency in recording and reconstructing polarization features from a single measurement offers a unique advantage for spatially resolved live imaging.
Review
Optics
Gary Brooker, Nisan Siegel
Summary: This article presents a chronicle of the 15-year development of Fresnel incoherent correlation holography (FINCH), from its initial description to its current state as a 3D microscopic imaging technique that can achieve optical resolution beyond the Rayleigh limit. The article describes the path from the original demonstration of FINCH to its current use in perfect imaging of multicolor fluorescent biological specimens and reference test patterns using fluorescence or reflected light imaging.
Article
Optics
Jakob G. P. Lingg, Thomas S. Bischof, Bernardo A. Arus, Emily D. Cosco, Ellen M. Sletten, Christopher J. Rowlands, Oliver T. Bruns, Andriy Chmyrov
Summary: The development of fluorophores with photoemission beyond 1000 nm provides the opportunity to develop novel fluorescence microscopes sensitive to those wavelengths. Imaging at wavelengths beyond the visible spectrum enables imaging depths of hundreds of microns in intact tissue, making this attractive for volumetric imaging applications.
LASER & PHOTONICS REVIEWS
(2023)
Article
Optics
Florian Strohl, Daniel Henry Hansen, Mireia Nager Grifo, Asa Birna Birgisdottir
Summary: Multifocus microscopy enables capturing entire volumes with a single exposure. This scalable method incorporates optical sectioning and improves axial resolution capabilities, making it suitable for imaging dense samples such as cells and tissues.
Article
Materials Science, Multidisciplinary
Zachary Hallenbeck, Esther A. Wertz
Summary: This work introduces a new technique that combines super-resolution microscopy with fluorescence lifetime imaging microscopy to study the enhancement of single-molecule decay rate by plasmonic nanoparticles. The technique provides high spatial resolution and temporal resolution, allowing for a better understanding of the mechanisms of plasmon-enhanced emission.
ADVANCED OPTICAL MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Guankui Wang, Markella Zannikou, Laren Lofchy, Yue Li, Hanmant Gaikwad, Irina Balyasnikova, Dmitri Simberg
Summary: The type of fluorescent label on liposomes significantly impacts their extravasation and accumulation in tumors. In animal experiments, indocarbocyanine lipids (ICLs) were found to spread and accumulate in tumor tissue more easily compared to fluorescent phospholipids (FPLs).
Article
Biochemical Research Methods
Yu-Chun Lin, Yuan Luo, Ying-Ju Chen, Huei-Wen Chen, Tai-Horng Young, Hsuan-Ming Huang
Summary: This study presents a single-shot quantitative differential phase-contrast (DPC) imaging method based on deep learning (DL) and color-encoded illumination. The DL-based model is trained to generate an isotropic quantitative phase image directly from a single-shot intensity measurement. The results show that the DL-based phase images are visually similar to the ground-truth phase images and have a high structural similarity index. This study demonstrates the feasibility of using DL to generate quantitative phase imaging from a single-shot intensity measurement.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Nanoscience & Nanotechnology
Yilin Luo, Luzhe Huang, Yair Rivenson, Aydogan Ozcan
Summary: Autofocusing is critical for high-quality microscopic imaging, with hardware-based and algorithmic methods being the main approaches. The deep learning-based offline autofocusing method Deep-R is significantly faster than standard online algorithmic autofocusing methods, allowing for rapid and blind autofocusing of microscope images.
Article
Chemistry, Multidisciplinary
Jun-Fang Wang, Chun-Miao Zhao, Jian-Xing Yang, Xu He, Xiao-Liu Li, Jin-Mei Li, Ke-Rang Wang
Summary: A mannose-modified perylene monoimide derivative PMI-Man has been developed, which exhibits highly selective binding to double-stranded DNA molecules, potent live/dead cell imaging, and histological imaging via both confocal and light microscopies. This approach may be utilized to create a universal colorful staining method for human tissues suitable for both confocal and light microscopies.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Yuan Luo, Cheng Hung Chu, Sunil Vyas, Hsin Yu Kuo, Yu Hsin Chia, Mu Ku Chen, Xu Shi, Takuo Tanaka, Hiroaki Misawa, Yi-You Huang, Din Ping Tsai
Summary: A dielectric Moire metalens is designed, fabricated, and implemented for fluorescence imaging with varifocal capabilities, utilizing two complementary phase metasurfaces with adjustable focal length through mutual angle tuning. The compact design may have important applications in clinical fluorescence microscopy and endoscopy.
Article
Microscopy
E. A. Ingerman, R. A. London, R. Heintzmann, M. G. L. Gustafsson
JOURNAL OF MICROSCOPY
(2019)
Article
Multidisciplinary Sciences
Benedict Diederich, Patrick Then, Alexander Juegler, Ronny Foerster, Rainer Heintzmann
Article
Chemistry, Physical
Martin Reifarth, Walter Mueller, Blerina Shkodra-Pula, Helmar Goerls, Ulrich S. Schubert, Rainer Heintzmann, Stephanie Hoeppener
PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
(2019)
Article
Optics
Christian Karras, Maria Smedh, Ronny Foerster, Hendrik Deschout, Julia Fernandez-Rodriguez, Rainer Heintzmann
OPTICS COMMUNICATIONS
(2019)
Article
Optics
Christian Karras, Mario Chemnitz, Rainer Heintzmann, Markus A. Schmidt
Article
Multidisciplinary Sciences
Walter Hauswald, Ronny Foerster, Juergen Popp, Rainer Heintzmann
Article
Biochemical Research Methods
Tonmoy Chakraborty, Meghan K. Driscoll, Elise Jeffery, Malea M. Murphy, Philippe Roudot, Bo-Jui Chang, Saumya Vora, Wen Mai Wong, Cara D. Nielson, Hua Zhang, Vladimir Zhemkov, Chitkale Hiremath, Estanislao Daniel De la Cruz, Yating Yi, Ilya Bezprozvanny, Hu Zhao, Raju Tomer, Rainer Heintzmann, Julian P. Meeks, Denise K. Marciano, Sean J. Morrison, Gaudenz Danuser, Kevin M. Dean, Reto Fiolka
Article
Multidisciplinary Sciences
Andreas Markwirth, Mario Lachetta, Viola Moenkemoeller, Rainer Heintzmann, Wolfgang Huebner, Thomas Huser, Marcel Mueller
NATURE COMMUNICATIONS
(2019)
Article
Biochemical Research Methods
Shih-Ya Chen, Rainer Heintzmann, Christoph Cremer
BIOMEDICAL OPTICS EXPRESS
(2019)
Article
Multidisciplinary Sciences
Benedict Diederich, Barbora Marsikova, Brad Amos, Rainer Heintzmann
Proceedings Paper
Optics
Alexander Juegler, Jan Becker, Ronny Foerster, Rainer Heintzmann
ADVANCES IN MICROSCOPIC IMAGING II
(2019)
Meeting Abstract
Ophthalmology
Thomas Ach, Christina Wobbe, Rainer Heintzmann, Jost Hillenkamp, Christine Curcio, Kenneth R. Sloan, Katharina Bermond
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2019)
Proceedings Paper
Engineering, Biomedical
Neel Dey, Shijie Li, Katharina Bermond, Rainer Heintzmann, Christine A. Curcio, Thomas Ach, Guido Gerig
MEDICAL IMAGING 2019: IMAGE PROCESSING
(2019)
Meeting Abstract
Cell Biology
P. M. McCall, K. Kim, J. Wang, A. Poznyakovskiy, B. Diederich, A. W. Fritsch, M. Kreysing, R. Heintzmann, J. Guck, S. Alberti, J. Brugues, A. A. Hyman
MOLECULAR BIOLOGY OF THE CELL
(2018)
Article
Ophthalmology
Katharina Bermond, Christina Wobbe, Ioana-Sandra Tarau, Rainer Heintzmann, Jost Hillenkamp, Christine A. Curcio, Kenneth R. Sloan, Thomas Ach
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2020)