Article
Polymer Science
Hoyeol Lee, Jin Myoung Yoo, Seung Yun Nam
Summary: This study utilized extrusion-based bioprinting to fabricate HA/gelatin composite scaffolds with large weight fractions of HA, aiming to provide a biomimetic environment for bone tissue regeneration. The results showed that the bioprinted scaffolds exhibited excellent shape fidelity, mechanical strength comparable to native bone, and enhanced bioactivity for cell proliferation and osteogenic differentiation.
Article
Materials Science, Ceramics
Aylin M. Deliormanli, Mertcan Ensoylu, Ghada ALMisned, H. O. Tekin
Summary: In this study, polycaprolactone (PCL) and polylactide-co-glycolide (PLGA) coated bioactive glass scaffolds containing molybdenum disulfide (MoS2) nanoparticles were prepared and evaluated for their usability in bone tissue engineering applications. The results showed that the presence of MoS2 nanoparticles in the polymer matrix improved the mechanical properties of the scaffolds and increased the hydroxyapatite-forming ability of the bioactive glass-based composites. Additionally, the composite scaffolds demonstrated high radiation-shielding ability due to the presence of MoS2 nanoparticles.
CERAMICS INTERNATIONAL
(2023)
Article
Polymer Science
Asmaa M. Abd El-Aziz, Ahmed Abd El-Fattah, Azza El-Maghraby, Doaa A. Ghareeb, Sherif Kandil
Summary: Chemical cross-linking was used to create nanocomposite hydrogels composed of gelatin and borosilicate bioactive glass with different content. The nanocomposite scaffolds showed promising properties such as hydrophilicity, biodegradability, increased compressive modulus, and facilitated apatite formation in simulated body fluid. The nanocomposite hydrogels exhibited a self-standing elastic structure with greater storage modulus than loss modulus.
Article
Biotechnology & Applied Microbiology
Jianhang Du, Huifeng Ding, Shengyang Fu, Dejian Li, Bin Yu
Summary: In this study, a bifunctional Bi-BG scaffold for near-infrared (NIR)-activated photothermal ablation of bone tumors and enhanced bone defect regeneration is fabricated. The Bi-BG scaffolds exhibit outstanding photothermal performance under NIR irradiation and effectively inhibit tumor growth while promoting bone regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Amany A. Mostafa, Azza A. Mahmoud, Mohamed A. Abdel Hamid, Mona Basha, Mohamed S. El-Okaily, Abdel Fattah A. Abdelkhalek, Mohamed I. El-Anwar, Sara El Moshy, Amr Gibaly, Elham A. Hassan
Summary: The study confirmed the potential osteogenic activity of chitosan/polyvinyl alcohol-based scaffolds loaded with risedronate and nBG. The scaffolds promote cell proliferation, alkaline phosphatase activity, and calcium deposition for bone defect healing. Increasing chitosan and nBG in the scaffolds decreased drug release, increased scaffold strength, supported cell proliferation, and promoted bone formation.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Mozhgan Keshavarz, Parvin Alizadeh
Summary: The use of alginate-coated 58S BG scaffolds enhances viability and osteogenic differentiation of hMSCs, leading to accelerated osteogenic gene expression and increased antibacterial properties, making it a promising candidate for bone regeneration.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Materials Science, Ceramics
Chenglong Wang, Chunyang Meng, Zhuo Zhang, Qingsan Zhu
Summary: This study used bioactive glass to improve the bone repair scaffold material made of polycaprolactone (PCL) and found that increasing the content of bioactive glass can enhance the hydrophilicity of the scaffold, improve cell adhesion and proliferation, and promote bone repair. The results of the study suggest that PCL scaffolds containing 20% bioactive glass have great potential for clinical bone repair.
CERAMICS INTERNATIONAL
(2022)
Article
Materials Science, Multidisciplinary
Micaela Degli Esposti, Maryam Changizi, Roberta Salvatori, Luigi Chiarini, Valeria Cannillo, Davide Morselli, Paola Fabbri
Summary: This study compares the effects of using different biopolymers and bioactive inorganic particles to create porous scaffolds. The addition of inorganic fillers is found to have a positive effect on cell proliferation and metabolic activity, especially when amorphous bioactive glass is added.
ACS APPLIED POLYMER MATERIALS
(2022)
Article
Chemistry, Physical
Jian Xiao, Qianghua Wei, Jinhong Xue, Zhicong Liu, Zhen Li, Zifa Zhou, Fu Chen, Fulai Zhao
Summary: In this study, a nanocomposite scaffold composed of mesoporous bioactive glass/bacterial cellulose (MBG/BC) was synthesized using an in situ membrane-liquid interface culture. The incorporation of MBG into BC scaffolds enhanced their bioactivity, as demonstrated by in vitro mineralization experiments. Furthermore, the MBG/BC nanocomposite scaffolds promoted human bone marrow stromal cell proliferation, alkaline phosphatase activity, and osteogenic-related gene expression, indicating their potential for bone defect treatment.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Materials Science, Ceramics
Shi Jixiang, Zhai Dong, Zhu Min, Zhu Yufang
Summary: This study investigated a novel BG-MnO2 composite scaffold, finding that it has the ability to scavenge H2O2 and supply O-2 simultaneously, showing great potential for bone tissue regeneration.
JOURNAL OF INORGANIC MATERIALS
(2022)
Article
Materials Science, Biomaterials
Jin Qi, Yili Wang, Liping Chen, Linjie Chen, Feng Wen, Lijiang Huang, Pfukwa Rueben, Chunwu Zhang, Huaqiong Li
Summary: Large size bone defects are a global health problem, and 3D printing technology is being used to prepare multifunctional scaffolds for bone reconditioning and regeneration. A study has demonstrated that a 3D-printed scaffold made with polydopamine (PDA) decoration and the sustained release of bioactive ions improved surface bioactivity and promoted better osteogenesis and angiogenesis. This finding could provide a valuable basis for custom implants in extensive bone defect repair applications.
REGENERATIVE BIOMATERIALS
(2023)
Review
Biochemistry & Molecular Biology
Xiaojing Mo, Dianjian Zhang, Keda Liu, Xiaoxi Zhao, Xiaoming Li, Wei Wang
Summary: This review summarizes the classification of n-HAp composites, the application of n-HAp composite scaffolds loaded with bioactive factors and drugs in bone tissue engineering, the drug loading methods of n-HAp composite scaffolds, and prospects the research direction of n-HAp composite scaffolds in the future.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Ceramics
Chao-Kuang Kuo, Hsiang-Wei Huang, Liu-Gu Chen, Yu-Jen Chou
Summary: The study prepared Sr-BG/chitosan composite scaffolds using freeze drying technique and characterized the microstructure, swelling, degradation behaviors, in vitro bioactivity, and cytotoxicity. The results demonstrated the potential of this composite scaffold for tissue engineering applications with improved bioactivity and cell viability.
JOURNAL OF ASIAN CERAMIC SOCIETIES
(2021)
Article
Engineering, Biomedical
Christian Polley, Thomas Distler, Caroline Scheufler, Rainer Detsch, Henrik Lund, Armin Springer, Dominik Schneidereit, Oliver Friedrich, Aldo R. Boccaccini, Hermann Seitz
Summary: Bone healing is a complex process that involves various factors such as mechanical, chemical, and electrical cues. Scientists worldwide aim to create synthetic biomaterials that combine these factors to achieve tailored and controlled tissue regeneration. This study presents a method using additive manufacturing techniques to create macroporous biomaterial scaffolds made of a piezoelectric and bioactive ceramic-crystallized glass composite. These scaffolds show suitable mechanical strength, bioactivity, and piezoelectric properties, making them promising candidates for bone tissue engineering.
MATERIALS TODAY BIO
(2023)
Article
Engineering, Biomedical
Aylin Kara, Thomas Distler, Christian Polley, Dominik Schneidereit, Hermann Seitz, Oliver Friedrich, Funda Tihminlioglu, Aldo R. Boccaccini
Summary: This study demonstrates the feasibility of using decellularized bone particles as reinforcement agents in gelatin-based scaffolds for bone tissue engineering. The fabricated 3D scaffolds showed homogenous distribution of the reinforcement agents and exhibited enhanced mechanical properties and degradation rates. The scaffolds also demonstrated bioactivity and cytocompatibility, making them potential candidates for bone tissue engineering.
MATERIALS TODAY BIO
(2022)
Review
Engineering, Biomedical
Pingping Han, Guillermo A. Gomez, Georg N. Duda, Saso Ivanovski, Patrina S. P. Poh
Summary: The dynamics of cell mechanics and epigenetic signatures play a crucial role in cell behavior and fate, affecting the outcomes of tissue regeneration. While 2D geometric substrates have been widely used to study cell mechanics in response to the extracellular microenvironment, there is limited knowledge about cell mechanobiology and epigenetics in 3D biomaterial matrices. This review explores the potential of additive manufacturing for incorporating multi length-scale geometry features on scaffolds, and discusses how scaffold geometry influences cell and nuclear mechanosensing and epigenetic modifications, ultimately impacting tissue regeneration outcomes.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Wei S. Tan, Axel C. Moore, Molly M. Stevens
Summary: This study explores the design requirements for mimicking articular cartilage by developing in silico models and fitting them to experimental data, and suggests that fiber-reinforced poroelastic materials are essential for physiological poroelastic mimics of articular cartilage.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Omar Rifaie-Graham, Jonathan Yeow, Adrian Najer, Richard Wang, Rujie Sun, Kun Zhou, Tristan N. Dell, Christopher Adrianus, Chalaisorn Thanapongpibul, Mohamed Chami, Stephen Mann, Javier Read de Alaniz, Molly M. Stevens
Summary: This study describes a non-equilibrium nanosystem that utilizes light-gated chemical communication, controllable feedback, and macroscopic oscillations. The system allows for communication between nanoreactors and induces chemical mechanical oscillations in a hydrogel using feedback loops.
Article
Biotechnology & Applied Microbiology
Taras Sych, Jan Schlegel, Hanna M. G. Barriga, Miina Ojansivu, Leo Hanke, Florian Weber, R. Beklem Bostancioglu, Kariem Ezzat, Herbert Stangl, Birgit Plochberger, Jurga Laurencikiene, Samir El Andaloussi, Daniel Fuerth, Molly M. Stevens, Erdinc Sezgin
Summary: Lipid nanoparticles, viruses, exosomes and liposomes can be characterized using fluorescence fluctuations analysis. A method called single-particle profiler is introduced to provide information on the content and biophysical properties of thousands of particles within the size range of 5-200 nm. The single-particle profiler is utilized to measure mRNA encapsulation efficiency of lipid nanoparticles, viral binding efficiencies of different nanobodies, and the biophysical heterogeneity of liposomes, lipoproteins, exosomes and viruses.
NATURE BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Sebastien J. P. Callens, Daniel Fan, Ingmar A. J. van Hengel, Michelle Minneboo, Pedro J. Diaz-Payno, Molly M. Stevens, Lidy E. Fratila-Apachitei, Amir A. Zadpoor
Summary: The organization of bone cells in landscapes with varying curvatures was studied, revealing a preference for local concavities, multicellular bridging, and collective stress fiber orientation.
NATURE COMMUNICATIONS
(2023)
Article
Engineering, Biomedical
Hongya Geng, Emily J. Lupton, Yun Ma, Rujie Sun, Christopher L. Grigsby, Giulia Brachi, Xiaorui Li, Kun Zhou, Daniel J. Stuckey, Molly M. Stevens
Summary: The development of near-infrared light responsive conductive polymers provides a useful theranostic platform for malignant tumors. The self-assembly of ultrathin 2D polypyrrole nanosheets with tunable size distribution enables photoacoustic imaging and photothermal therapy. The pH-responsive features of the nanosheets show potential for accurate and highly efficient theranostics.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Anu K. Solanki, Helene Autefage, Antonio R. Rodriguez, Shweta Agarwal, Joaquin Penide, Muzamir Mahat, Thomas Whittaker, Amy Nommeots-Nomm, Elena Littmann, David J. Payne, Anthony D. Metcalfe, Felix Quintero, Juan Pou, Molly M. Stevens, Julian R. Jones
Summary: In this study, novel glass fibers were developed to improve chronic wound healing by activating the HIF pathway. The results showed that the glass fibers released therapeutic ions, including cobalt, which greatly promoted the expression of angiogenic genes. This suggests the potential use of these fibers in chronic wound dressings.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Pharmacology & Pharmacy
Catherine Saunders, Camille A. de Villiers, Molly M. Stevens
Summary: Nanoparticles have great potential in medicine, but their manufacturing and quality control present challenges to their clinical translation. Advancements in single particle techniques can help overcome these challenges and facilitate the development of future nanoformulations.
Article
Chemistry, Multidisciplinary
Kaja I. Ritzau-Reid, Sebastien J. P. Callens, Ruoxiao Xie, Martina Cihova, Daniel Reumann, Christopher L. Grigsby, Lino Prados-Martin, Richard Wang, Axel C. Moore, James P. K. Armstrong, Juergen A. Knoblich, Molly M. Stevens
Summary: 3D organoids are widely used in vitro models for studying human development and disease. However, the current culture methods have limitations in terms of reproducibility and geometric control. A new approach combining stem cell biology and bioengineering is presented, using melt electrospinning writing to generate grid scaffolds that guide the self-organization of pluripotent stem cells. This method allows for high-throughput generation, culture, and analysis of organoids, reducing the time and labor involved in traditional methods.
ADVANCED MATERIALS
(2023)
Article
Multidisciplinary Sciences
Junfang Zhang, Yuntao Zhu, Christian Njel, Yuxin Liu, Pietro Dallabernardina, Molly M. Stevens, Peter H. Seeberger, Oleksandr Savateev, Felix F. Loeffler
Summary: In this study, a new synthesis strategy for carbon nitride photoelectrodes was introduced through advanced film generation techniques. This method involved spin coating pretreatment of a conductive substrate with a multipurpose polymer and a supramolecular precursor, followed by chemical vapor deposition to synthesize dual-layer carbon nitride photoelectrodes. The polymer improved the polymerization degree and electrical conductivity of carbon nitride, leading to state-of-the-art photoelectrochemical performance and high yields in C-H functionalization.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Daniel W. Hagey, Miina Ojansivu, Beklem R. Bostancioglu, Osama Saher, Jeremy P. Bost, Manuela O. Gustafsson, Roberto Gramignoli, Mathias Svahn, Dhanu Gupta, Molly M. Stevens, Andre Goergens, Samir EL Andaloussi
Summary: This study provides important insights into the cellular response to extracellular vesicles (EVs), showing that EV dose has a more significant effect than cell source. At high doses, exocytosis is down-regulated and lysosomal activity is up-regulated. However, at low doses, specific responses based on EV cell source are observed, reflecting the activities of the EV's source cells. Furthermore, immune cell-derived EVs are most associated with recipient cells.
Article
Biotechnology & Applied Microbiology
Tamanna Rahman, Saman Tavana, Nicoleta Baxan, Kay A. Raftery, George Morgan, Thomas P. Schaer, Nigel Smith, Axel Moore, Jonathan Bull, Molly M. Stevens, Nicolas Newell
Summary: This study non-invasively quantified 3D strains within the intervertebral disc (IVD) during different stages of nucleus replacement surgery and found that nucleus removal resulted in disc height loss and increased circumferential strains, which were restored by the nucleus replacement device (NRD). The findings support nucleus replacement surgery as a viable treatment option and provide insights for future clinical trials.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Mairim Russo Serafini, Alexandra Mowat, Susanah Mustafa, Siamak Saifzadeh, Tara Shabab, Onur Bas, Nicholas O'Rourke, Dietmar W. Hutmacher, Flavia Medeiros Savi
Summary: This study evaluated the use of mPCL scaffolds loaded with PRP for hernia repair in sheep models, and found that the mPCL scaffolds enhanced soft tissue regeneration. After 6 months, the mPCL scaffolds showed similar mechanical properties, collagen, and vascular ingrowth compared to PP meshes. This pilot study supports the use of mPCL as a safe and effective biodegradable scaffold for hernia and vaginal prolapse repair.
BIOENGINEERING-BASEL
(2023)
Article
Biochemical Research Methods
Vernon LaLone, Aleksandra Aizenshtadt, John Goertz, Froydis Sved Skottvoll, Marco Barbero Mota, Junji You, Xiaoyu Zhao, Henriette Engen Berg, Justyna Stokowiec, Minzhi Yu, Anna Schwendeman, Hanne Scholz, Steven Ray Wilson, Stefan Krauss, Molly M. Stevens
Summary: Confocal Raman spectral imaging (RSI) enables label-free visualization of molecules in biological specimens without sample preparation. qRamanomics, an integrated bioanalytical methodology, is developed to quantify the deconvoluted spectra for quantitative spatial chemotyping of biomolecules. It is applied to assess specimen variation and maturity in 3D liver organoids and identify biomolecular response signatures from liver-altering drugs, facilitating drug metabolism and accumulation monitoring.
CELL REPORTS METHODS
(2023)
Article
Chemistry, Multidisciplinary
Jules Lee, Prajakatta Mulay, Matthew J. Tamasi, Jonathan Yeow, Molly M. Stevens, Adam J. Gormley
Summary: Oxygen tolerant polymerizations allow for high-throughput synthesis of diverse polymer architectures. The development of a robotic platform enables fully automated PET-RAFT polymerizations with individual control of reactions. This platform successfully synthesizes various polymers with high conversions and low dispersity. With the inclusion of machine learning protocols, this robotic platform has the potential to become a self-driving lab for dispensing, synthesizing, and monitoring large polymer libraries.