期刊
VIRUSES-BASEL
卷 10, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/v10080419
关键词
swine influenza virus; interleukin-1 beta; NLRP3 inflammasome; receptor-interacting protein kinase 1; dynamin-related protein 1; mitochondrial fission; porcine alveolar macrophage
类别
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada
Nucleotide-binding domain and leucine-rich repeat-containing protein 3 (NLRP3) inflammasome plays a pivotal role in modulating lung inflammation in response to the influenza A virus infection. We previously showed that the swine influenza virus (SIV) infection induced NLRP3 inflammasome-mediated IL-1 production in primary porcine alveolar macrophages (PAMs), and we were interested in examining the upstream signaling events that are involved in this process. Here, we report that the SIV-infection led to dynamin-related protein 1 (DRP1) phosphorylation at serine 579 and mitochondrial fission in PAMs. IL-1 production was dependent on the reactive oxygen species (ROS) production, and DRP1 phosphorylation resulted in the upregulation of the NLRP3 inflammasome. Furthermore, the requirement of the kinase activity of receptor-interacting protein kinase 1 (RIPK1) for the IL-1 production and RIPK1-DRP1 association suggested that RIPK1 is an upstream kinase for DRP1 phosphorylation. Our results reveal a critical role of the RIPK1/DRP1 signaling axis, whose activation leads to mitochondrial fission and ROS release, in modulating porcine NLRP3 inflammasome-mediated IL-1 production in SIV-infected PAMs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据