Article
Biochemistry & Molecular Biology
Vladimir A. Morozov, Alexey V. Morozov
Summary: The study investigates the transmission and immune response of human endogenous retrovirus HML.2 between cells, revealing its potential pathogenic mechanism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Virology
Jin-Hui Zhu, Gaurav Rawal, Ethan Aljets, Wannarat Yim-Im, Yong-Le Yang, Yao-Wei Huang, Karen Krueger, Phillip Gauger, Rodger Main, Jianqiang Zhang
Summary: A 5-plex real-time RT-PCR method was developed for simultaneous detection and differentiation of four swine enteric coronaviruses. The method had high specificity, sensitivity, and repeatability. SADS-CoV was not detected in the tested samples, but PEDV was frequently detected.
Article
Microbiology
J. N. Brazelton de Cardenas, C. D. Garner, Y. Su, L. Tang, R. T. Hayden
Summary: This study compared four methods for detecting antibiotic resistance genes in Enterobacterales isolates and found that commercially available PCR-based assays can accurately identify antimicrobial resistance loci in clinically significant Gram-negative bacteria, with OpGen Acuitas AMR assay showing the highest overall concordance with sequencing results.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Li Liu, Yan-Zhen Chen, Shu-Sheng Zhang, Xiu-Ping Chen, Guo-Qiang Lin, Hang Yin, Chen-Guo Feng, Fang Zhang
Summary: A new strategy for isotope-coded doubly charged labeling of endogenous guanidino compounds (GCs) was developed, showing good linear responses and low detection limits. The method demonstrated reliability and accuracy, making it suitable for the analysis and study of endogenous GCs in various lung tissues.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Yongshun Zhou, Fanze Meng, Kai Han, Kaiyue Zhang, Jianfeng Gao, Fulong Chen
Summary: This study evaluated the expression stability of 9 endogenous reference genes of Chlorella sp. TLD 6B, a microalgae growing in the Taklamakan Desert, Xinjiang of China, under four abiotic stresses (drought, salt, cold and heat). The results showed that 18S, GTP, IDH and CYP were the most stable endogenous reference genes under different stress conditions, and UBC and 18S were the most suitable endogenous reference gene combinations for all samples. Using these reliable endogenous reference genes ensures more accurate RT-qPCR quantification, facilitating functional genomics studies of desert Chlorella and the mining of resistance genes.
SCIENTIFIC REPORTS
(2023)
Article
Infectious Diseases
Eric Farfour, M. Asso-Bonnet, M. Vasse
Summary: The ID NOW COVID-19 assay shows promising features of rapid and accurate diagnostic capability, with the ability to be repeated efficiently using samples stored in UTM. However, there were a small number of false negative results. Interpretation of test results should consider clinical and epidemiological context.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Chemistry, Analytical
Tamara Kutateladze, Kakha Bitskinashvili, Nelly Sapojnikova, Tamar Kartvelishvili, Nino Asatiani, Boris Vishnepolsky, Nelly Datukishvili
Summary: This study developed an effective multi-allergen diagnostic approach for assessing transgenic soybeans, using multiplex PCR coupled with DNA chip technology. The method was shown to be efficient for simultaneous identification of major allergens in herbicide-resistant GMOs, providing a fast and accurate testing method for food allergens.
Article
Public, Environmental & Occupational Health
Valeria Caramello, Alessandra Macciotta, Fabrizio Bar, Alessandro Mussa, Anna Maria De Leo, Alessandro Vincenzo De Salve, Fabio Nota, Carlotta Sacerdote, Fulvio Ricceri, Adriana Boccuzzi
Summary: For emergency department patients with COVID-19-like symptoms and negative RT-PCR results, a comprehensive clinical evaluation combined with lung ultrasound and computed tomography can help detect COVID-19 patients with false-negative RT-PCR results.
Article
Plant Sciences
Miftakhurohmah, Kikin Hamzah Mutaqin, Bonny Poernomo Wahyu Soekarno, Dono Wahyuno, Sri Hendrastuti Hidayat
Summary: The study identified both endogenous and episomal forms of PYMoV in black pepper plants, with more endogenous forms than episomal forms. Both forms were successfully detected in leaf and berry samples. Berries from the same plant confirmed the presence of both forms, with identical sequences in leaves and berries from the same plant.
AUSTRALASIAN PLANT PATHOLOGY
(2021)
Review
Medicine, General & Internal
Jeffrey Justin Margret, Sushil K. Jain
Summary: Gene optimizations are crucial for maintaining biological functions, and gene dysregulation can lead to disease progression. Advanced analytical techniques help establish connections between impaired genes and disease conditions. This knowledge can be used to design clinical trials for novel therapeutic interventions and disease prevention.
CURRENT MEDICAL RESEARCH AND OPINION
(2023)
Article
Multidisciplinary Sciences
Noa Wolff, Michaela Hendling, Fabian Schroeder, Silvia Schoenthaler, Andreas F. Geiss, Branka Bedenic, Ivan Barisic
Summary: Antibiotic resistances have led to treatment failures at an alarming rate, prompting the urgent need for obtaining as much information as possible for effective medication.
SCIENTIFIC REPORTS
(2021)
Article
Veterinary Sciences
Gyu-Tae Jeon, Hye-Ryung Kim, Yeun-Kyung Shin, Oh-Kyu Kwon, Hae-Eun Kang, Oh-Deog Kwon, Choi-Kyu Park
Summary: In this study, a duplex real-time quantitative RT-PCR assay was developed for reliable detection of canine parainfluenza virus 5. The assay exhibited high sensitivity, specificity, and accuracy. Clinical evaluation results showed that it had superior diagnostic performance compared to previous assays.
VETERINARY SCIENCES
(2023)
Article
Microbiology
Mohamed M. Taw, Magnus Bertelsen, Mohamed A. Abdel-Rahman, Peter N. Strong, Keith Miller
Summary: The search for novel antimicrobials has led to the discovery of AMPs derived from scorpion and snake venoms, such as Smp24 and Smp43. These peptides have shown broad-spectrum antimicrobial activity against bacterial pathogens. By understanding the mechanisms by which these AMPs affect bacterial cells, researchers can modify their structure to develop more effective drugs in the future.
Article
Medicine, General & Internal
Dipti Pattnaik, Nirmala Poddar, Basanti K. Pathi, Kumudini Panigrahi, Smaranita Sabat, Ankita Roy, A. Raj K. Patro, Amrut Mohapatra, Shubhransu Patro, Ashok K. Praharaj
Summary: This study evaluated the performance of the cartridge-based Abbott ID NOW test for SARS-CoV-2 detection by comparing it to the standard probe-based real-time RT-PCR method. The ID NOW test showed lower sensitivity but higher specificity, positive and negative predictive values compared to RT-PCR. It can be used as a point-of-care test for COVID-19 due to its ease of use and short result time.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Chitrangada Das Mukhopadhyay, Pramita Sharma, Koel Sinha, Keshav Rajarshi
Summary: The global outbreak of COVID-19 due to SARS-CoV-2 has created an unprecedented humanitarian crisis, calling for a sensitive, specific, fast, and affordable diagnostic technique. Modern technologies such as CRISPR-based detection platforms or microarray kits have shown enhanced sensitivity and specificity, offering promising solutions for efficient disease management.
BIOPHYSICAL CHEMISTRY
(2021)
Article
Cell Biology
P. Robert Beatty, Henry Puerta-Guardo, Sarah S. Killingbeck, Dustin R. Glasner, Kaycie Hopkins, Eva Harris
SCIENCE TRANSLATIONAL MEDICINE
(2015)
Article
Microbiology
Michael A. Schmid, Dustin R. Glasner, Sanjana Shah, Daniela Michlmayr, Laura D. Kramer, Eva Harris
Article
Microbiology
Henry Puerta-Guardo, Dustin R. Glasner, Eva Harris
Article
Virology
Dustin R. Glasner, Henry Puerta-Guardo, P. Robert Beatty, Eva Harris
ANNUAL REVIEW OF VIROLOGY, VOL 5
(2018)
Article
Microbiology
Dustin R. Glasner, Kalani Ratnasiri, Henry Puerta-Guardo, Diego A. Espinosa, P. Robert Beatty, Eva Harris
Article
Ecology
Dana M. Hardbower, Jessica L. Dolman, Dustin R. Glasner, Joseph A. Kendra, Kurt E. Williamson
AQUATIC MICROBIAL ECOLOGY
(2012)
Article
Virology
Dhanya K. Williams, Teresa A. Galvin, Yamei Gao, Christina O'Neill, Dustin Glasner, Arifa S. Khan
JOURNAL OF VIROLOGY
(2013)
Article
Virology
Hailun Ma, Teresa A. Galvin, Dustin R. Glasner, Syed Shaheduzzaman, Arifa S. Khan
JOURNAL OF VIROLOGY
(2014)
Article
Immunology
Mayuri Sharma, Dustin R. Glasner, Heather Watkins, Henry Puerta-Guardo, Yoseph Kassa, Michael A. Egan, Hansi Dean, Eva Harris
JOURNAL OF INFECTIOUS DISEASES
(2020)
Article
Cell Biology
Henry Puerta-Guardo, Dustin R. Glasner, Diego A. Espinosa, Scott B. Biering, Mark Patana, Kalani Ratnasiri, Chunling Wang, P. Robert Beatty, Eva Harris
Article
Immunology
Henry Puerta-Guardo, Takako Tabata, Matthew Petitt, Milena Dimitrova, Dustin R. Glasner, Lenore Pereira, Eva Harris
JOURNAL OF INFECTIOUS DISEASES
(2020)
Article
Microbiology
Chunling Wang, Henry Puerta-Guardo, Scott B. Biering, Dustin R. Glasner, Edwina B. Tran, Mark Patana, Trent A. Gomberg, Carmel Malvar, Nicholas T. N. Lo, Diego A. Espinosa, Eva Harris
Article
Biology
Thomas P. Burke, Patrik Engstrom, Cuong J. Tran, Ingeborg M. Langohr, Dustin R. Glasner, Diego A. Espinosa, Eva Harris, Matthew D. Welch
Summary: Mice lacking both interferon receptors provide a tractable model for investigating rickettsiosis, virulence factors, and immunity. The study shows that the actin-based motility factor Sca2 is required for dissemination from the skin to internal organs, and the outer membrane protein OmpB contributes to eschar formation, revealing potential live-attenuated vaccine candidates. Differences in interferon signaling may underlie the discrepancies in susceptibility to rickettsial pathogens between mice and humans.