Article
Food Science & Technology
Yoah Moon, Sangha Han, Jeong Won Son, Si Hong Park, Sang-Do Ha
Summary: The study found that peroxyacetic acid treatment was more effective in disinfecting murine norovirus compared to ultraviolet-C irradiation. Furthermore, sequential treatment with UV-C and PAA did not show a synergistic effect in reducing MNV-1 titers.
Article
Virology
Gabriela N. Condezo, Carmen San Martin
Summary: The localization of viral nucleic acids in the cell is crucial for understanding the infectious cycle. By using nucleotide analogs such as BrdU, researchers can accurately distinguish between host and viral DNA during replication or transcription. Proper experimental conditions and methodological variations are necessary to ensure effective incorporation of labeling agents and accurate viral genome localization in infected cells.
Review
Biotechnology & Applied Microbiology
Kelvin C. M. Lee, Jochen Guck, Keisuke Goda, Kevin K. Tsia
Summary: The biophysical properties of cells provide crucial information about their identities, homeostatic state, and disease pathogenesis, which can now be accessed through advances in biophysical cytometry. To fully unleash the potential of biophysical cytometry, further research and standardization are needed in exploring cellular biophysical content and protocols. Overcoming these barriers will not only lead to a better mechanistic understanding of biological systems, but also help identify new cost-effective disease biomarkers.
TRENDS IN BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Vyankatesh Raml Kudkyal, Iori Matsuura, Hiroaki Hiramatsu, Kyoko Hayashi, Toshio Kawahara
Summary: Grape seed extract (GSE) has been found to have virucidal activity against human norovirus, suggesting its potential use in treating norovirus infections.
Article
Engineering, Mechanical
Sebastian Sachs, Manuel Ratz, Patrick Maeder, Jorg Koenig, Christian Cierpka
Summary: The astigmatism particle tracking velocimetry is extended by a deterministic algorithm and a deep neural network (DNN) to include size classification of particles of multimodal size distribution. By extracting particle images from the experimentally acquired images and placing them on a synthetic background, semi-synthetic images with consistent ground truth are generated. The present contribution paves the way for quantitative evaluation of microfluidic separation and mixing processes.
EXPERIMENTS IN FLUIDS
(2023)
Review
Chemistry, Multidisciplinary
Jens B. Simonsen, Emil B. Kromann
Summary: Fluorescence-based techniques are widely used in nanomedicine research, but are rarely quantitative, making direct comparisons across studies difficult. This Commentary focuses on mechanisms of fluorescence-labeled nanoparticles and highlights pitfalls in quantitative fluorescence experiments that are often overlooked.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Biochemistry & Molecular Biology
Hugo Voisin, Estelle Bonnin, Melanie Marquis, Camille Alvarado, Suzanne Lafon, Teresa Lopez-Leon, Frederic Jamme, Isabelle Capron
Summary: This study utilized cellulose nanocrystals (CNCs) to establish a model system for studying proteins in microdroplets created by a microfluidic setup. The proteins, bovine serum albumin (BSA) and a GH7 endoglucanase, relevant to food and plant science, were investigated. The results showed that BSA disturbed the overall structure of the droplets, while GH7 interacted with the CNCs without disrupting the system organization. These findings pave the way for the development of more complex polysaccharide-protein in-vitro models.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Multidisciplinary Sciences
Matteo Bernardello, Emilio J. Gualda, Pablo Loza-Alvarez
Summary: Light-sheet fluorescence microscopy is an important tool used in biological and biomedical research. However, the sample mounting procedure can be cumbersome and time-consuming. This article presents a new imaging paradigm for LSFM that offers multimodal imaging and a straightforward sample mounting strategy, enhancing the flexibility and throughput of the system.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Shiyi Cheng, Sipei Fu, Yumi Mun Kim, Weiye Song, Yunzhe Li, Yujia Xue, Ji Yi, Lei Tian
Summary: The new label-free imaging technique developed in this study utilizing deep learning can accurately predict subcellular features and improve throughput for imaging cytometry, enabling applications in phenotyping, pathology, and high-content screening.
Article
Chemistry, Multidisciplinary
Angela M. R. Mantovanelli, Oleksandr Glushonkov, Virgile Adam, Jip Wulffele, Daniel Thedie, Martin Byrdin, Ingo Gregor, Oleksii Nevskyi, Joerg Enderlein, Dominique Bourgeois
Summary: The switching mechanism of genetically encoded fluorescent proteins at cryogenic temperature is different from that at ambient temperature, which enhances the understanding of cryo-SMLM and provides potential improvements for effective labeling efficiency with this technique.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemistry & Molecular Biology
Marta Z. Pacia, Natalia Chorazy, Magdalena Sternak, Benedikt Fels, Michal Pacia, Mariusz Kepczynski, Kristina Kusche-Vihrog, Stefan Chlopicki
Summary: In this study, the role of Rac1 in TNF-induced vascular inflammation was investigated. It was found that Rac1 mediated the formation of lipid droplets and nanostructural alterations associated with vascular inflammation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Chemistry, Physical
Laura I. FitzGerald, Angus P. R. Johnston
Summary: The review discusses current approaches for measuring the cellular uptake of nanoparticles and proteins, with a focus on recent advances in sensor technology. It highlights the requirements for quantifying internalization and potential pitfalls in data analysis. The importance of understanding recycling of internalized material back to the cell surface and methods for quantifying this process are also explored.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Cell Biology
Simone Pelicci, Laura Furia, Pier Giuseppe Pelicci, Mario Faretta
Summary: The modern fluorescence microscope combines different technologies with varying performance levels. However, the best results are achieved by maximizing one parameter while compromising others, which limits the adoption of new optical microscopy tools in research labs.
Review
Chemistry, Organic
Thorge Reiber, Oleksandr Zavoiura, Christian Dose, Dmytro A. Yushchenko
Summary: Cell analysis techniques like flow cytometry and fluorescence microscopy require bright and specific staining reagents to generate strong fluorescence signal. Significant research has been done in the field of fluorophore development, but modifying biomolecules with multiple emitters remains challenging.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Immunology
Claudia Andrade, Barbara Oliveira, Silvia Guatelli, Pedro Martinez, Beatriz Simoes, Claudia Bispo, Cinzia Ferrario, Francesco Bonasoro, Jose Rino, Michela Sugni, Rui Gardner, Rita Zilhao, Ana Varela Coelho
Summary: In this study, coelomocytes present in the coelomic fluid of Marthasterias glacialis were characterized using a combination of flow cytometry, imaging flow cytometry, and fluorescence plus transmission electron microscopy. Two distinct coelomocyte populations were identified and studied in terms of abundance, morphology, ultrastructure, cell viability, and cell cycle profiles. The integrative experimental workflow allowed for a better understanding of the heterogeneity within these coelomocyte populations.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Public, Environmental & Occupational Health
M. E. I. Elmandy, M. E. Magri, L. A. Garcia, G. Fongaro, C. R. M. Barardi
INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH
(2018)
Article
Water Resources
Lucas Ariel T. Garcia, Leandro Martin T. Garcia, Celia Regina M. Barardi
Article
Chemistry, Medicinal
Thais Moreira Osorio, Franco Delle Monache, Louise Domeneghini Chiaradia, Alessandra Mascarello, Taisa Regina Stumpf, Carlos Roberto Zanetti, Douglas Bardini Silveira, Celia Regina Monte Barardi, Elza de Fatima Albino Smania, Aline Viancelli, Lucas Ariel Totaro Garcia, Rosendo Augusto Yunes, Ricardo Jose Nunes, Artur Smania
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2012)
Article
Biotechnology & Applied Microbiology
A. de Abreu Correa, D. S. M. Souza, V. Moresco, C. R. Kleemann, L. A. T. Garcia, C. R. M. Barardi
JOURNAL OF APPLIED MICROBIOLOGY
(2012)
Article
Environmental Sciences
L. A. T. Garcia, A. Viancelli, C. Rigotto, M. R. Pilotto, P. A. Esteves, A. Kunz, C. R. M. Barardi
JOURNAL OF WATER AND HEALTH
(2012)
Article
Environmental Sciences
V. Moresco, A. Viancelli, M. A. Nascimento, D. S. M. Souza, A. P. D. Ramos, L. A. T. Garcia, C. M. O. Simoes, C. R. M. Barardi
MARINE POLLUTION BULLETIN
(2012)
Article
Veterinary Sciences
A. Viancelli, L. A. T. Garcia, A. Kunz, R. Steinmetz, P. A. Esteves, C. R. M. Barardi
RESEARCH IN VETERINARY SCIENCE
(2012)
Article
Veterinary Sciences
A. Viancelli, L. A. T. Garcia, M. Schiochet, A. Kunz, R. Steinmetz, J. R. Ciacci-Zanella, P. A. Esteves, C. R. M. Barardi
RESEARCH IN VETERINARY SCIENCE
(2012)
Article
Environmental Sciences
Lucas Ariel Totaro Garcia, Laurita Boff, Celia Regina Monte Barardi, Markus Nagl
FOOD AND ENVIRONMENTAL VIROLOGY
(2019)
Review
Engineering, Environmental
B. L. S. Freitas, U. C. Terin, N. M. N. Fava, P. M. F. Maciel, L. A. T. Garcia, R. C. Medeiros, M. Oliveira, P. Fernandez-Ibanez, J. A. Byrne, L. P. Sabogal-Paz
Summary: Household slow sand filters (HSSF) are effective and promising point-of-use water treatment methods, with improvements made to enhance performance and user acceptability. Various HSSF models are now available, including alternatives designs and constant operation ones, in addition to the patented ones.
Review
Public, Environmental & Occupational Health
A. M. Nielsen, L. A. T. Garcia, K. J. S. Silva, L. P. Sabogal-Paz, M. M. Hincapie, L. J. Montoya, L. Galeano, A. Galdos-Balzategui, F. Reygadas, C. Herrera, S. Golden, J. A. Byrne, P. Fernandez-Ibanez
Summary: Chlorination has been commonly used for providing safe drinking water, but there are challenges in effective implementation. A literature review focusing on three Latin American countries found that guidance on household-based chlorination may not incentivize users enough. Moreover, variations in water quality are often overlooked in recommending chlorine doses. User preferences, chlorine product quality, and availability also pose barriers to the sustainable use of chlorination.
INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH
(2022)
Article
Biochemical Research Methods
Valdinei L. Belini, Natalia de Melo Nasser Fava, Lucas Ariel Totaro Garcia, Maria Julia Rodrigues da Cunha, Lyda Patricia Sabogal-Paz
Summary: In this study, a cost-effective in situ microscope system was assessed to acquire images of Giardia cysts without using any chemical labels or sample preparation steps. An image analysis algorithm analyzes the acquired images, and automatically enumerates and provides morphological information of cysts within 10 min. The proposed system has the potential to be an alternative technique for the characterization of Giardia cyst suspensions.
JOURNAL OF MICROBIOLOGICAL METHODS
(2022)
Article
Environmental Sciences
Lucas A. T. Garcia, Fernando L. Silva, Barbara L. S. Freitas, Natalia N. M. Fava, Fermin Reygadas, Lyda P. Sabogal-Paz
Summary: Low-cost household technologies for water treatment are crucial in improving drinking water quality. This study assessed the efficiency of a multi-barrier household water treatment system in removing physical-chemical and bacteriological parameters from river water. The results showed that this system was effective in improving water quality and reducing the risks of microbial contamination, ensuring safe drinking water.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Article
Engineering, Environmental
Lucas Ariel Totaro Garcia, Celia Regina Monte Barardi