Article
Food Science & Technology
Kapil K. Chousalkar, Samiullah Khan, Andrea R. McWhorter
Summary: In the past decade, significant progress has been made in egg quality and safety research within the scientific community, particularly focusing on nontyphoidal Salmonella. Cold storage is effective in limiting bacterial replication on and in eggs, but there is a lack of prescriptive guidelines for egg storage in the retail market in many countries. While the risk of foodborne virus transmission via table eggs is low, the rise in cage-free egg production has led to an increased risk of fungal contamination. Probiotics or vitamin supplementation can enhance egg defense by altering the caecal microbiota, though further research is needed on how indicator bacteria are related to egg quality.
CURRENT OPINION IN FOOD SCIENCE
(2021)
Article
Food Science & Technology
Xiaojie Qin, Yanhong Liu, Xianming Shi
Summary: The deletion of acrD in Salmonella Enteritidis decreases its resistance to egg white and results in cell lysis, while having no significant effect on adhesion and invasion ability compared to the wild type strain.
Article
Physics, Applied
Maike Moritz, Claudia Wiacek, Thomas Weihe, Joerg Ehlbeck, Klaus-Dieter Weltmann, Peggy G. Braun
Summary: In the European Union, foodborne outbreaks caused by Salmonella Enteritidis related to eggs have been reported in 2018. Cold atmospheric pressure plasma has shown promise as a decontamination method for eggs, achieving significant reductions in bacterial counts and effectively inactivating Salmonella Enteritidis. Overall, the study indicates potential for realistic implementation of an industrial prototype plasma source.
PLASMA PROCESSES AND POLYMERS
(2021)
Article
Agriculture, Multidisciplinary
Marie-Francoise Cochet, Florence Baron, Sylvie Bonnassie, Sophie Jan, Nadine Leconte, Julien Jardin, Valerie Briard-Bion, Michel Gautier, Simon C. Andrews, Catherine Guerin-Dubiard, Francoise Nau
Summary: Recent research has shown that egg white at 45 degrees C has powerful bactericidal activity against S. enterica serovar Enteritidis, partly due to two specific peptides. However, these peptides only contribute to part of the bactericidal activity, suggesting the presence of other yet to be determined antimicrobial factors.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Food Science & Technology
Bin Liu, Wanwan Hou, Ke Li, Qing Chen, Yaxin Liu, Tianli Yue
Summary: The gene SEN1393 enables Salmonella enterica serovar Enteritidis to survive better in egg white compared to other pathogens by regulating the sulfate assimilation pathway, reducing oxidative toxicity, and improving ion absorption.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Food Science & Technology
Yue Yi, Ahmed G. Abdelhamid, Yumin Xu, Ahmed E. Yousef
Summary: The study identified lytic Salmonella phages isolated from livestock farms in Ohio, USA, which showed activities against various Salmonella serovars, including Typhimurium and Enteritidis. The phages were found to be viable in acidic and alkaline environments as well as at temperatures ranging from 4 to 55 degrees Celsius. Combination of phage treatment and mild heat resulted in significant reductions in Salmonella Typhimurium and Salmonella Enteritidis levels in liquid whole eggs.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
J. Allen, B. Balasubramanian, K. Rankin, T. Shah, A. M. Donoghue, I. Upadhyaya, B. Sartini, Y. Luo, A. Upadhyay
Summary: Salmonella Enteritidis is a food-borne pathogen that causes enteric illnesses in humans, primarily through contaminated poultry meat and eggs. Despite traditional disinfection approaches, egg-borne outbreaks continue to occur. This study investigated the efficacy of a Trans-cinnamaldehyde nanoemulsion as an egg wash treatment to reduce Salmonella Enteritidis on shelled eggs, and found promising results.
Article
Veterinary Sciences
Hassan Bahramianfard, Abdollah Derakhshandeh, Zahra Naziri, Reza Khaltabadi Farahani
Summary: The research revealed a high contamination rate of Salmonella in poultry and egg samples, with some isolates showing resistance to multiple antibiotics, posing a potential threat to human health.
BMC VETERINARY RESEARCH
(2021)
Article
Microbiology
Xia Meng, Mengping He, Binjie Chen, Pengpeng Xia, Jinqiu Wang, Chunhong Zhu, Heng Wang, Guoqiang Zhu
Summary: RyhB-1 and RyhB-2 are small non-coding RNAs that regulate iron homeostasis in Salmonella by sensing iron concentration in the environment. They inhibit the growth of Salmonella Typhimurium in macrophages by modulating the expression of genes sicA and rtsB. The regulatory role of RyhBs and their targets in Salmonella Enteritidis is not well understood. We found that RyhB paralogs are expressed when Salmonella Enteritidis interacts with chicken macrophages. They downregulate survival-related virulence factors and attenuate the survival of Salmonella Enteritidis inside macrophages.
Article
Biotechnology & Applied Microbiology
F. Campioni, C. N. Gomes, A. M. M. Bergamini, D. P. Rodrigues, M. R. Tiba-Casas, J. P. Falcao
Summary: This study compared the ability of Salmonella enterica serovars Enteritidis and Dublin to invade, survive within, and stimulate cytokine release in various host cells. It found that compared to Enteritidis, Dublin has a stronger ability to survive within macrophages and induces weaker cytokine production, potentially explaining the differences in disease incidence caused by these two serovars.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Food Science & Technology
Asma Entezari, Sahar Roshanak, Golshan Shakeri, Nasser Sedaghat
Summary: Eggs are crucial for human nutrition, but they can also transmit salmonellosis. This study found that coating eggs with zein can improve their quality and effectively control Salmonella contamination.
JOURNAL OF FOOD SCIENCE
(2022)
Article
Immunology
Lina Song, Jia Wu, Kaiqi Weng, Fenghua Yao, Wanwipa Vongsangnak, Guoqiang Zhu, Guohong Chen, Yu Zhang, Qi Xu
Summary: The effector protein Hcp plays an important role in SE invasion, induction of dGCs apoptosis, triggering immune responses, and increasing egg contamination. Key host response regulators to bacterial Hcp were identified using quantitative proteomics.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biology
Sreeparna Majee, Atish Roy Chowdhury, Roven Pinto, Ankur Chattopadhyay, Amey Nitin Agharkar, Dipshikha Chakravortty, Saptarshi Basu
Summary: The study combines various methods to investigate the drying process of bacterial droplets, demonstrating long-term pathogenesis of pathogens and providing a holistic approach to understand bacterial pattern formation and pathogenesis. Experimental results show that mechanical stress during the drying process reduces bacterial viability, but induces hyper-proliferation in macrophages, thereby increasing pathogenicity.
COMMUNICATIONS BIOLOGY
(2021)
Article
Food Science & Technology
Hongfei Zhang, Sabrina Peiqi Chen, Hon Luen Seck, Weibiao Zhou
Summary: This study found that low-energy X-ray effectively inactivated biofilm cells of Salmonella Enteritidis and Pseudomonas fluorescens on shell eggs, reducing extracellular polysaccharide content. Egg white proteins and shell texture remained unchanged after irradiation, and no harmful substances were detected in irradiated eggs.
Article
Infectious Diseases
Frank Alleweldt, Senda Kara, Kris Best, Frank M. Aarestrup, Martin Beer, Theo M. Bestebroer, Josefina Campos, Gabriele Casadei, Isabel Chinen, Gary Van Domselaar, Catherine Dominguez, Helen E. Everett, Ron Am Fouchier, Kathie Grant, Jonathan Green, Dirk Hoper, Jonathan Johnston, Marion Pg Koopmans, Bas B. Oude Munnink, Robert Myers, Celine Nadon, Ami Patel, Anne Pohlmann, Stefano Pongolini, Aleisha Reimer, Shane Thiessen, Claudia Wylezich
Summary: WGS is found to be between 1.2 and 4.3 times more expensive than routine methods per sample, but it provides major benefits in pathogen identification and surveillance, changing laboratory workflows, analytical processes, and outbreak detection and control significantly.
Article
Gastroenterology & Hepatology
Rita Matos, Hugo Santos Sousa, Jorge Nogueiro, Ana Magalhaes, Celso A. Reis, Fatima Carneiro, Irina Amorim, Freddy Haesebrouck, Fatima Gartner
Summary: The study shows that gastric non-Helicobacter pylori Helicobacter can bind to the human gastric mucosa, with a prevalence of chronic gastritis and chronic active gastritis among patients. Interestingly, these bacteria were found to bind to the gastric mucosa of individuals with severe chronic inflammation and intestinal metaplasia.
Review
Infectious Diseases
N. J. C. Woittiez, J. van Prehn, F. van Immerseel, E. Goossens, M. P. Bauer, C. L. Ramspek, R. M. E. Slangen, I. M. Purmer, J. Ludikhuize
Summary: Clostridium perfringens infection with intravascular haemolysis is rare but often fatal. Toxinotyping is not regularly performed, and increased use is advocated for better understanding of pathophysiology and more effective interventions.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Microbiology
Bruno Toledo-Silva, Lisa Beuckelaere, Anneleen De Visscher, Chloe Geeroms, Evelyne Meyer, Sofie Piepers, Damien Thiry, Freddy Haesebrouck, Sarne De Vliegher
Summary: This paper presents a new quantitative method for evaluating and quantifying the in vitro growth inhibition of mastitis-related bacteria. The method can accurately quantify the differences in growth inhibition among different NAS isolates.
Article
Health Care Sciences & Services
Rita Matos, Emily Taillieu, Sofie De Bruyckere, Chloe De Witte, Alexandra Rema, Hugo Santos-Sousa, Jorge Nogueiro, Celso A. Reis, Fatima Carneiro, Freddy Haesebrouck, Irina Amorim, Fatima Gaertner
Summary: This study reveals the presence of Helicobacter spp. in 46.3% of gastric cancer and obese patients, with 43.8% having single H. pylori infection and 2.5% having mixed infections with H. pylori and H. felis associated with dogs or cats. The effectiveness of current Helicobacter eradication treatments needs further improvement.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Microbiology
Lore Van Damme, Chana Callens, Michelle Dargatz, Monika Flugel, Sarah Hark, Frank Thiemann, Stefan Pelzer, Richard Ducatelle, Filip Van Immerseel, Evy Goossens
Summary: This study shows the important role of sialidases in the pathogenesis of necrotic enteritis caused by NetB producing Clostridium perfringens. Sialidases degrade the intestinal mucus layer and increase toxin production. Additionally, the use of recombinant sialidase increases bacterial adherence to specific cells.
VETERINARY MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Evelien Biebaut, Ilias Chantziaras, Filip Boyen, Bert Devriendt, Freddy Haesebrouck, Charles-Oliver Gomez-Duran, Dominiek Maes
Summary: This study investigated the presence of Mycoplasma hyopneumoniae in the breeding population of ten Belgian farrow-to-finish farms. The results showed that the prevalence of M. hyopneumoniae PCR varied greatly between farms, with gilts being more likely to be infected than sows. Positive animals were mostly found at 30-40 days of gestation. This highlights the importance of a sufficiently long quarantine period and proper gilt acclimation practices before introducing gilts to the sow herd.
PORCINE HEALTH MANAGEMENT
(2022)
Article
Physiology
Annatachja De Grande, Richard Ducatelle, Saskia Leleu, Christof Rapp, Cibele Torres, Massimiliano Petracci, Stefaan De Smet, Joris Michiels, Freddy Haesebrouck, Filip Van Immerseel, Evelyne Delezie
Summary: This study evaluated the effect of different zinc sources and vitamin E levels on meat yield and quality in broilers subjected to chronic cyclic heat stress. The results showed that replacing ZnSO4 with ZnAA increased breast meat weight and yield, as well as improved the water-holding capacity of the meat. The study also found that the beneficial effects of ZnAA were independent of the vitamin E level.
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Junhua Xie, Freddy Haesebrouck, Lien Van Hoecke, Roosmarijn E. Vandenbroucke
Summary: Bacterial extracellular vesicles (bEVs) have been shown to play a crucial role in the pathogenesis of various diseases. This article thoroughly discusses the contribution of bEVs in disease pathogenesis, their potential as diagnostic biomarkers, and their application as therapeutic targets.
TRENDS IN MICROBIOLOGY
(2023)
Article
Infectious Diseases
Francisco Cortez Nunes, Emily Taillieu, Teresa Letra Mateus, Silvia Teixeira, Freddy Haesebrouck, Irina Amorim
Summary: This study aimed to assess the presence of antibiotic resistance genes previously reported in Helicobacter pylori in gastric samples from 36 pigs where DNA of H. pylori-like organisms had been detected. Based on PCR and sequencing analysis, two samples were positive for the 16S rRNA mutation gene conferring tetracycline resistance, and one sample was positive for the frxA gene conferring metronidazole resistance. These findings indicate the potential occurrence of acquired antimicrobial resistance in H. pylori-like organisms associated with pigs.
Article
Veterinary Sciences
Francisco Cortez Nunes, Teresa Letra Mateus, Emily Taillieu, Silvia Teixeira, Nuno Carolino, Alexandra Rema, Sofie De Bruyckere, Fatima Gartner, Freddy Haesebrouck, Irina Amorim
Summary: This study reveals the presence of Helicobacter species and F. gastrosuis in the stomach of pigs and wild boars, indicating a possible association with gastric disorders.
VETERINARY RESEARCH
(2022)
Meeting Abstract
Gastroenterology & Hepatology
Emily Taillieu, Sophie Rutten, Stijn Michiels, Yuna Arnst, Sofie De Bruyckere, Koen Chiers, Frauke van Aert, Heiko De Schepper, Emma Callewaert, Christophe George, Wouter Van Moerkercke, Glenn Vanneste, Nina Van Heddegem, Erik Vanderstraeten, Freddy Haesebrouck, Christophe Van Steenkiste
Review
Veterinary Sciences
Emily Taillieu, Koen Chiers, Irina Amorim, Fatima Gaertner, Dominiek Maes, Christophe Van Steenkiste, Freddy Haesebrouck
Summary: This article discusses the importance of Helicobacter species in dogs, cats, and pigs and their potential to infect humans. The article highlights the association of Helicobacter suis with gastritis and decreased weight gain in pigs, as well as its potential to cause disease in humans. It also mentions that multiple Helicobacter species can colonize the stomachs of dogs and cats, but their pathogenic significance is likely low. The article concludes that besides H. pylori, gastric Helicobacter species associated with these animals are also clinically relevant in humans.
VETERINARY RESEARCH
(2022)
Review
Gastroenterology & Hepatology
L. Capirchio, T-D. Huang, C. De Witte, F. Haesebrouck, C. Fervaille, C. Gillain, J-F. Rahier, T. De Ronde
Summary: This article reports the first case of elevated CA 19-9 related to H. suis gastritis, which normalized after eradication. In addition to H. pylori, infection with NHPH species can also cause elevated CA 19-9.
ACTA GASTRO-ENTEROLOGICA BELGICA
(2022)
Article
Veterinary Sciences
Silvia Teixeira, Dulce Filipe, Manuela Cerqueira, Patricia Barradas, Francisco Cortez Nunes, Fatima Faria, Freddy Haesebrouck, Joao R. Mesquita, Fatima Gartner, Irina Amorim
Summary: Cats serve as a natural reservoir for many non-Helicobacter pylori Helicobacters, and close contact with animals has been identified as a risk factor for gastric diseases caused by these bacteria. The prevalence of Helicobacter species in cats was determined, with Helicobacter heilmannii and Helicobacter salomonis being the most commonly detected species. Significant histopathological alterations associated with the presence of Helicobacter spp. were not observed in most cases, suggesting potential adaptation of these bacteria to the feline gastric microenvironment.
VETERINARY SCIENCES
(2022)
Article
Veterinary Sciences
Karina Sonalio, Henrique M. S. Almeida, Marina L. Mechler-Dreibi, Gabriel Y. Storino, Freddy Haesebrouck, Dominiek Maes, Luis Guilherme de Oliveira
Summary: The study found that Mycoplasma hyopneumoniae infection affects the diversity of respiratory microbiota and facilitates colonization of other pathogens. Differences in pulmonary and nasal microbiota were observed in pigs with different infection statuses.
VETERINARY RESEARCH
(2022)