期刊
ZOOLOGICAL SCIENCE
卷 30, 期 7, 页码 529-533出版社
ZOOLOGICAL SOC JAPAN
DOI: 10.2108/zsj.30.529
关键词
amphibian; tachykinin-like peptide; tree frog; Theloderma kwangsiensis; skin secretions
类别
资金
- Chinese National Natural Science Foundation [30830021, 31025025, 31000960, U1132601]
- Ministry of Science and Technology [2010CB529800, 2011ZX09102-002-10]
- Yunnan Province [2011CI139, 201213C009]
- Jiangsu Province [CXZZ11_0649, BE2012748]
Most amphibians belonging to Rhacophoridae have an arboreal life. A large amount of bioactive peptides have been identified from amphibian skin, but none from amphibians belonging to Rhacophoridae have been reported to date. A tachykinin-like peptide, tachykinin-Thel, was purified and characterized from skin secretions of the tree frog, Theloderma kwangsiensis. Its primary structure was determined as KPSPDRFYGLM-NH2 by Edman degradation and mass spectrometry analysis. cDNA sequence encoding the precursor of tachykinin-Thel was cloned from the skin cDNA library. Tachykinin-Thel induced the contraction of isolated ileum smooth muscle in a dose-dependent manner. The current work reported a bioactive peptide, tachykinin-Thel, from Rhacophoridae amphibians and confirmed the presence of tachykinin-like peptide in the skin of the tree frog, which may facilitate their arboreal life.
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