Review
Endocrinology & Metabolism
Xiaonan Ding, Xiaochen Wang, Junxia Du, Qiuxia Han, Dong Zhang, Hanyu Zhu
Summary: This systematic review focuses on the proteins in urinary extracellular vesicles (EV) and their potential role in diabetic nephropathy (DN). The findings suggest that urinary EV proteins may participate in DN through various pathways, providing insight into the mechanisms and potential biomarkers of this disease.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Medicine, Research & Experimental
Ye Feng, Xin Zhong, Hai-Feng Ni, Cui Wang, Tao-Tao Tang, Li-Ting Wang, Kai-Yun Song, Ri-Ning Tang, Hong Liu, Bi-Cheng Liu, Lin-Li Lv
Summary: The study revealed that urinary small EVs derived CCL21 mRNA may serve as an early biomarker for identifying DN linked with pathogenesis, and CCL21 mRNA-mediated T cell infiltration may constitute the key mechanism of chronic inflammation in DN.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Review
Medicine, General & Internal
Goren Saenz-Pipaon, Saioa Echeverria, Josune Orbe, Carmen Roncal
Summary: Diabetic kidney disease is the leading cause of end stage renal disease in developed countries, with a multifactorial pathogenesis leading to clinical manifestations such as proteinuria, hypertension, and gradual decline in kidney function. Traditional treatments can slow down DKD progression but cannot completely halt it, emphasizing the urgent need for novel early diagnosis and therapeutic targets.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Qing Li, Jiong Zhang, Yi Fang, Yan Dai, Ping Jia, Ziyan Shen, Sujuan Xu, Xiaoqiang Ding, Feng Zhou
Summary: This study identified phosphorylated proteins in urinary extracellular vesicles (uEVs) as potential biomarkers for diabetic nephropathy (DN) using ultra-high-sensitivity quantitative phosphoproteomics. The researchers detected 233 phosphopeptides within the uEVs, with 47 phosphoproteins showing significant alterations in DN patients. Further validation experiments confirmed the importance of phosphorylated aquaporin-2 and phosphorylated glycogen synthase kinase-3 beta in DN.
Article
Biotechnology & Applied Microbiology
Baoxu Lin, Jianhua Liu, Yue Zhang, Yabin Wu, Shixiao Chen, Yibo Bai, Qiuying Liu, Xiaosong Qin
Summary: Using urinary peptidomics, differences in protein expression were identified among healthy individuals, IMN patients, and patients with other kidney diseases. A total of 1,080 peptide-matched proteins were identified, 279 of which were differentially expressed in IMN patients, leading to the prediction of 32 proteases. Abnormal expression of these proteases in the glomeruli of IMN patients was confirmed, suggesting dysregulation of the calpain/matrix metalloproteinase/cathepsin axis in IMN.
Article
Biochemistry & Molecular Biology
Veronica Dimuccio, Linda Bellucci, Marianna Genta, Cristina Grange, Maria Felice Brizzi, Maddalena Gili, Sara Gallo, Maria Laura Centomo, Federica Collino, Benedetta Bussolati
Summary: Research has identified that miR145 and miR126 levels increase in urinary EVs from diabetic patients along with albuminuria, suggesting their role as biomarkers of damage progression and proteinuria development in diabetic nephropathy. These miRNAs were found to be specifically elevated during progression of diabetic nephropathy, highlighting their potential as biomarkers of epithelial to mesenchymal transition in kidney damage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Endocrinology & Metabolism
Dongfeng Gu, Yanan Ding, Xin Jiang, Beili Shen, Luca Musante, Harry Holthofer, Hequn Zou
Summary: This study investigated urinary extracellular vesicles (uEVs) in patients with diabetes with kidney injury. The results showed that the protein concentration of uEVs significantly increased in patients with diabetes with kidney injury, suggesting that diabetes with kidney injury may alter the abundance and cargo of uEVs.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Urology & Nephrology
Melissa Uil, Chi M. Hau, Mohamed Ahdi, James D. Mills, Jesper Kers, Moin A. Saleem, Sandrine Florquin, Victor E. A. Gerdes, Rienk Nieuwland, Joris J. T. H. Roelofs
Summary: This study found that diabetic patients with microalbuminuria and/or macroalbuminuria exhibited increased concentrations of total EVs and EVs from various cell types in the plasma. miR-99a-5p was upregulated in patients with macroalbuminuria and was shown to downregulate mTOR protein expression in cultured human podocytes.
CLINICAL KIDNEY JOURNAL
(2021)
Article
Oncology
Amiram Sananes, Itay Cohen, Irit Allon, Oshrit Ben-David, Raghda Abu Shareb, Ksenia M. Yegodayev, David Stepensky, Moshe Elkabets, Niv Papo
Summary: Targeted therapies for prostate, breast, and ovarian cancers are based on their activity against primary tumors rather than their anti-metastatic activity. There is an urgent need for new agents targeting the metastatic process. Mesotrypsin and KLK6 are attractive putative targets for therapeutic intervention.
MOLECULAR ONCOLOGY
(2023)
Article
Genetics & Heredity
Tina Levstek, Teo Mlinsek, Marija Holcar, Katja Goricar, Metka Lenassi, Vita Dolzan, Bojan Vujkovac, Katarina Trebusak Podkrajsek
Summary: Current biomarkers for Fabry nephropathy lack sensitivity in detecting early kidney damage and do not predict progression of nephropathy. However, analysis of urinary extracellular vesicles (uEVs) and their derived microRNAs (miRNAs) may be applicable in predicting the development and progression of nephropathy in Fabry disease. The total concentration and size of uEVs were not indicative of nephropathy presence or progression, while certain miRNAs may play a role in Fabry nephropathy but further studies are needed to confirm their significance.
Review
Pharmacology & Pharmacy
V. Marcel Alavi
Summary: The OMA1 protease in the mitochondrial inner membrane has potential as a drug target for complex diseases in the elderly. Its activation is related to cellular stress and apoptosis, and its inhibition can delay or prevent apoptosis in animal models. Understanding OMA1's structure and regulation is still limited, and technical challenges in OMA1 drug screens need to be addressed. Potent OMA1 inhibitors hold promise as a new class of cytoprotective medicines for disorders influenced by dysfunctional mitochondria.
PHARMACOLOGICAL RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Mohamed Amine Jmel, Hajer Aounallah, Chaima Bensaoud, Imen Mekki, Jindrich Chmelar, Fernanda Faria, Youmna M'ghirbi, Michalis Kotsyfakis
Summary: Protease inhibitors are regulatory proteins found in all kingdoms that play crucial roles in controlling proteases, with potential applications in human disease treatment and agriculture. PIs from tick salivary glands have unique pharmacological properties and are promising candidates for drug development, progressing to preclinical and clinical trials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Francisca Ugarte, Daniela Santapau, Vivian Gallardo, Carolina Garfias, Anahi Yizmeyian, Soledad Villanueva, Carolina Sepulveda, Jocelyn Rocco, Consuelo Pasten, Cinthya Urquidi, Gabriel Cavada, Pamela San Martin, Francisco Cano, Carlos E. Irarrazabal
Summary: This study evaluated early tubulointerstitial damage biomarkers in pediatric patients with T1DM and their correlation with classical DKD parameters. The findings suggest that urinary exosome-like extracellular vesicles can serve as a new source for early detection of tubular injury biomarkers of DKD in T1DM patients.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Review
Endocrinology & Metabolism
Wenguang Chang, Mengyang Li, Lin Song, Suo Miao, Wanpeng Yu, Jianxun Wang
Summary: This article reviews the role of small extracellular vesicles (sEVs) and noncoding RNAs in diabetes and its complications. Noncoding RNA sorting into sEVs is selective, and the function of sEVs varies according to their tissue origin. Understanding the roles of sEVs from different tissues is important for studying their molecular mechanisms and applying them as therapeutic agents for diabetes treatment.
MOLECULAR METABOLISM
(2022)
Article
Medicine, General & Internal
Tina Levstek, Bojan Vujkovac, Andreja Cokan Vujkovac, Katarina Trebusak Podkrajsek
Summary: This study aimed to identify uEV-derived miRNAs involved in the development and/or progression of Fabry nephropathy. The expression of miR-21-5p and miR-222-3p was significantly higher in patients with stable renal function and those with progressive nephropathy, while the expression of miR-30a-5p, miR-10b-5p, and miR-204-5p was significantly lower in patients with progressive nephropathy. The dysregulated miRNAs may be potentially associated with pathophysiological pathways in the kidney.
FRONTIERS IN MEDICINE
(2023)
Article
Nutrition & Dietetics
Natalie Z. M. Eichner, Nicole M. Gilbertson, Luca Musante, Sabrina La Salvia, Arthur Weltman, Uta Erdbrugger, Steven K. Malin
Article
Biochemical Research Methods
Xiaomeng Xu, Karina Barreiro, Luca Musante, Oliver Kretz, Hanfei Lin, Hequn Zou, Tobias B. Huber, Harry Holthofer
PROTEOMICS CLINICAL APPLICATIONS
(2019)
Article
Sport Sciences
Natalie Z. M. Eichner, Nicole M. Gilbertson, Emily M. Heiston, Luca Musante, Sabrina La Salvia, Arthur Weltman, Uta Erdbrugger, Steven K. Malin
MEDICINE AND SCIENCE IN SPORTS AND EXERCISE
(2020)
Article
Peripheral Vascular Disease
Miranda E. Good, Luca Musante, Sabrina La Salvia, Nancy L. Howell, Robert M. Carey, Thu H. Le, Brant E. Isakson, Uta Erdbruegger
Article
Multidisciplinary Sciences
Luca Musante, Sai Vineela Bontha, Sabrina La Salvia, Angela Fernandez-Pineros, Joanne Lannigan, Thu H Le, Valeria Mas, Uta Erdbrugger
SCIENTIFIC REPORTS
(2020)
Meeting Abstract
Endocrinology & Metabolism
Emily Heiston, Anna Ballantyne, Nathan Stewart, Luca Musante, Uta Erdbruegger, Steven K. Malin
Article
Neurosciences
Emily M. Heiston, Anna Ballantyne, Sabrina La Salvia, Luca Musante, Uta Erdbrugger, Steven K. Malin
Summary: Acute exercise can decrease the levels of extracellular vesicles (EVs) in adults with obesity, thereby improving insulin sensitivity and vascular function.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Article
Endocrinology & Metabolism
Emily M. Heiston, Anna Ballantyne, Nathan R. Stewart, Sabrina La Salvia, Luca Musante, Joanne Lanningan, Uta Erdbrugger, Steven K. Malin
Summary: Insulin can reduce the levels of extracellular vesicles and is associated with insulin sensitivity, substrate oxidation, and inflammation. In individuals with metabolic syndrome and obesity, insulin can decrease the levels of medium-sized extracellular vesicles and improve metabolic flexibility.
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2022)
Article
Physiology
Tristan J. Ragland, Emily M. Heiston, Anna Ballantyne, Nathan R. Stewart, Sabrina La Salvia, Luca Musante, Melissa A. Luse, Brant E. Isakson, Uta Erdbrugger, Steven K. Malin
Summary: Metabolic Syndrome increases the risk of cardiovascular disease. Extracellular vesicles (EVs) are found to be important mediators of insulin sensitivity, but there are few studies on vascular function in humans. This study investigated the effect of insulin on EVs and vascular function in adults with MetS. The results suggest that EVs may play a role in vascular insulin sensitivity and disease risk.
PHYSIOLOGICAL REPORTS
(2023)
Meeting Abstract
Sport Sciences
Eric Trillaud, Uta Erdbrugger, Luca Musante, Sabrin La Salvia, Emily M. Heiston, Nathan R. Stewart, Steven K. Malin
MEDICINE & SCIENCE IN SPORTS & EXERCISE
(2021)
Meeting Abstract
Sport Sciences
Emily M. Heiston, Anna Ballantyne, Sabrina LaSalvia, Luca Musante, Uta Erdbruegger, Steven K. Malin
MEDICINE AND SCIENCE IN SPORTS AND EXERCISE
(2021)
Article
Cell Biology
Uta Erdbrugger, Charles J. Blijdorp, Irene V. Bijnsdorp, Francesc E. Borras, Dylan Burger, Benedetta Bussolati, James Brian Byrd, Aled Clayton, James W. Dear, Juan M. Falcon-Perez, Cristina Grange, Andrew F. Hill, Harry Holthofer, Ewout J. Hoorn, Guido Jenster, Connie R. Jimenez, Kerstin Junker, John Klein, Mark A. Knepper, Erik H. Koritzinsky, James M. Luther, Metka Lenassi, Janne Leivo, Inge Mertens, Luca Musante, Eline Oeyen, Maija Puhka, Martin E. van Royen, Catherine Sanchez, Carolina Soekmadji, Visith Thongboonkerd, Volkert van Steijn, Gerald Verhaegh, Jason P. Webber, Kenneth Witwer, Peter S. T. Yuen, Lei Zheng, Alicia Llorente, Elena S. Martens-Uzunova
Summary: Urine is commonly used for clinical diagnosis and biomedical research, and the discovery of extracellular vesicles (EV) in urine has opened a new scientific field. However, methods for isolating and characterizing urinary extracellular vesicles (uEVs) need further optimization and standardization to advance scientific research and translation into clinical practice. Recommendations for improved rigor, reproducibility, and interoperability in uEV research are provided to address current challenges and gaps in the field.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Physiology
Sabrina La Salvia, Luca Musante, Joanne Lannigan, Joseph Christopher Gigliotti, Thu H. Le, Uta Erdbrugger
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2020)
Meeting Abstract
Biochemistry & Molecular Biology
Miranda Elizabeth Good, Luca Musante, Sabrina LaSalvia, Robert M. Carey, Nancy L. Howell, Thu H. Le, Uta Erdbruegger, Brant E. Isakson
Meeting Abstract
Peripheral Vascular Disease
Sabrina La Salvia, Luca Musante, Joanne Lannigan, Robert M. Carey, Brant Isakson, Thu H. Le, Uta Erdbrugger