Article
Biochemistry & Molecular Biology
Benjamin Groth, Yi-Ching Lee, Chi-Chun Huang, Matilda McDaniel, Katie Huang, Lan-Hsuan Lee, Su-Ju Lin
Summary: The histone deacetylases Hst1 and Rpd3 are shown to link the regulation of the BNA genes involved in de novo NAD(+) metabolism with the PHO pathway through the Bas1-Pho2 and Pho2-Pho4 transcription activator complexes. The study provides insights into the complex interplay between two different aspects of cellular metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Urology & Nephrology
Martin R. Spaeth, K. Johanna R. Hoyer-Allo, Lisa Seufert, Martin Hoehne, Christina Lucas, Theresa Bock, Lea Isermann, Susanne Brodesser, Jan-Wilm Lackmann, Katharina Kiefer, Felix C. Koehler, Katrin Bohl, Michael Ignarski, Petra Schiller, Marc Johnsen, Torsten Kubacki, Franziska Grundmann, Thomas Benzing, Aleksandra Trifunovic, Marcus Krueger, Bernhard Schermer, Volker Burst, Roman-Ulrich Mueller
Summary: By studying the kidneys of mice under conditions of hypoxia and caloric restriction (CR), researchers discovered the significant role of the KYNU gene in the nephroprotective effect of CR. They also found that CR induces a novel pathway for NAD+ synthesis, which was also observed in humans.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2023)
Article
Cell Biology
Melissa H. Broeks, Nils W. F. Meijer, Denise Westland, Marjolein Bosma, Johan Gerrits, Hannah M. German, Jolita Ciapaite, Clara D. M. van Karnebeek, Ronald J. A. Wanders, Fried J. T. Zwartkruis, Nanda M. Verhoeven-Duif, Judith J. M. Jans
Summary: The malate-aspartate shuttle (MAS) plays a crucial role in de novo serine biosynthesis, and serine supplementation could be a potential therapeutic strategy for MAS defects and other redox disorders.
Article
Biochemistry & Molecular Biology
Xiaojing Wei, Chuanfei Wei, Yutian Tan, Ximing Dong, Zhao Yang, Jianqun Yan, Xiao Luo
Summary: This study aimed to determine the regulation of NAD+-related enzymes in the liver and their relationships with glucose and lipid metabolism. It was found that both high-fat diet feeding and calorie restriction increased hepatic NAD+ levels and the expression of related genes. In addition, high-fat diet feeding and calorie restriction had different effects on hepatic lipogenesis and gluconeogenesis. These findings highlight the importance of hepatic NAD+ metabolism in maintaining metabolic flexibility.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2023)
Article
Multidisciplinary Sciences
William R. Blevins, Jorge Ruiz-Orera, Xavier Messeguer, Bernat Blasco-Moreno, Jose Luis Villanueva-Canas, Lorena Espinar, Juana Diez, Lucas B. Carey, M. Mar Alba
Summary: Recent research has identified 213 de novo originated transcripts in Saccharomyces cerevisiae, with half of them expressed from regions that already contain other genes. These transcripts show similar expression changes in response to stress and some are capable of translating small proteins.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Yong Ding, Xinli Li, Geoff P. Horsman, Pengwei Li, Min Wang, Jine Li, Zhilong Zhang, Weifeng Liu, Bian Wu, Yong Tao, Yihua Chen
Summary: Nicotinamide adenine dinucleotide (NAD(+)) is a crucial molecule in biological processes and a de novo quinolinic acid pathway, named the C3N pathway, has been discovered as an alternative route for NAD(+) biosynthesis. The C3N pathway shows potential in cofactor engineering and higher production of desired compounds in cell factories.
Article
Microbiology
Yulia I. Deryabina, Anastasia S. Kokoreva, Olga I. Klein, Natalya N. Gessler, Elena P. Isakova
Summary: The study analyzes the dynamics of lipid profiles in Endomyces magnusii yeast during long-term cultivation using different substrates. It found that storage lipids are mainly composed of Di-(DAGs), TAGs, and FFAs, while membrane lipids are dominated by PE, St, PC, and PA. Calorie restriction leads to a decrease in storage lipids and an increase in membrane lipids. The study also highlights the important role of St, PA, and unsaturated fatty acids in membrane lipids, and the support of autophagic processes for maintaining high levels of lipids.
Article
Multidisciplinary Sciences
Alysha K. Lee, Jeremy H. Wei, Paula V. Welander
Summary: The marine myxobacterium Enhygromyxa Salina can produce cholesterol and undergo further modifications, similar to eukaryotes. However, complete demethylation at the C-4 position is achieved through unique bacterial proteins.
NATURE COMMUNICATIONS
(2023)
Article
Geriatrics & Gerontology
Yu Sun, Ruofan Yu, Hao-Bo Guo, Hong Qin, Weiwei Dang
Summary: The study revealed similar proteomic features between aged cells and cells undergoing caloric restriction, including induction of stress response pathways, suggesting that caloric restriction pathways are activated in aged cells. Additionally, the abnormal changes in mitochondria membrane proteins and proteolytic cellular state in old cells have also drawn attention.These proteomic analyses help identify potential genes and pathways that have causal effects on longevity.
Editorial Material
Multidisciplinary Sciences
Shaunak Deota, Satchidananda Panda
Summary: By combining calorie restriction, fasting, and regulating circadian rhythms, mice can achieve a long and healthy life.
Article
Chemistry, Physical
Ke Jiang, Li Li, Shuxin Liu, Yudong Xu, Jiayao Yang, Wanying Chu, Ping Lu, Ting Gao, Ruoxuan Bai, Fangxu Xu, Hongxin Zhao
Summary: In this study, mutants of E. aerogenes were constructed using different strategies to enhance hydrogen production by regulating NADH supply. The hydrogen yields of the mutants IAM1183-E, IAM1183-L, and IAM1183-EL increased by 23.3%, 81.7%, and 97.9%, respectively, compared to the wildtype strain. Overexpression of the pncB gene also led to increased hydrogen yields in the mutants. The strain IAM1183-EL/P exhibited the highest production efficiency with a 58% higher total hydrogen yield than the wildtype. Metabolite analysis revealed that the mutants with nuoE and ldhA gene deletions, combined with pncB overexpression, resulted in a redistribution of metabolic fluxes, leading to improved hydrogen yield.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2022)
Article
Cell Biology
Qingling Yang, Hui Li, Huan Wang, Wenhui Chen, Xinxin Zeng, Xiaoyan Luo, Jianmin Xu, Yingpu Sun
Summary: Recent research has emphasized the significant role of NAD(+) in ovarian aging. However, the impact of de novo NAD(+) biosynthesis on ovarian aging is still unknown. This study demonstrates that genetic ablation of Ido1 or Qprt, critical genes in de novo NAD(+) biosynthesis, leads to decreased ovarian NAD(+) levels in middle-aged mice, resulting in subfertility, irregular estrous cycles, reduced ovarian reserve, and accelerated aging. Additionally, impaired oocyte quality and mitochondrial metabolism were observed in mutant mice, but supplementation with nicotinamide riboside improved these reproductive outcomes.
Review
Microbiology
Kai Zhang
Summary: Leishmania parasites rely on different sources of lipids during their life cycle stages in the sand fly vector and vertebrate host, reflecting their growth rate and metabolic activity. Future studies may reveal how Leishmania uptake and remodel host lipids at cellular and molecular levels, and blocking this lipid transfer could be a novel strategy to control Leishmania growth.
CURRENT OPINION IN MICROBIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Joseph W. H. Burnett, Ziying Sun, Jianwei Li, Xiaonan Wang, Xiaodong Wang
Summary: The comprehensive life cycle assessment of NAD(P)H dependent biotransformations reveals that the synthesis of catalysts, particularly the use of noble metals and energy consumption, plays a dominant role in environmental impacts. Further research on cleaner and more sustainable catalyst alternatives is needed to regenerate the cofactor NAD(P)H.
Review
Biochemistry & Molecular Biology
Ekaterina Sergeeva, Tatiana Ruksha, Yulia Fefelova
Summary: The risk of malignant tumor development is increasing worldwide, with obesity being a known risk factor. Obesity is associated with metabolic alterations that promote cancerogenesis, including increased levels of estrogens, chronic inflammation, and hypoxia. Calorie restriction has been shown to improve the condition of patients with various diseases by influencing metabolism, hormone levels, and cell processes. It can regulate signal cascades that affect cancer cell proliferation, migration, survival, apoptosis, and response to chemotherapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Instruments & Instrumentation
T. J. Ognibene, At. Thomas, P. F. Daley, G. Bench, K. W. Turteltaub
NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS
(2015)
Article
Instruments & Instrumentation
T. J. Ognibene, K. W. Haack, G. Bench, T. A. Brown, K. W. Turteltaub
NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS
(2015)
Article
Instruments & Instrumentation
A. D. McCartt, T. Ognibene, G. Bench, K. Turteltaub
NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS
(2015)
Article
Chemistry, Analytical
A. Daniel McCartt, Ted J. Ognibene, Graham Bench, Kenneth W. Turteltaub
ANALYTICAL CHEMISTRY
(2016)
Article
Chemistry, Medicinal
Erin P. Madeen, Ted J. Ognibene, Richard A. Corley, Tammie J. McQuistan, Marilyn C. Henderson, William M. Baird, Graham Bench, Ken W. Turteltaub, David E. Williams
CHEMICAL RESEARCH IN TOXICOLOGY
(2016)
Article
Food Science & Technology
Jessica M. Hummel, Erin P. Madeen, Lisbeth K. Siddens, Sandra L. Uesugi, Tammie McQuistan, Kim A. Anderson, Kenneth W. Turteltaub, Ted J. Ognibene, Graham Bench, Sharon K. Krueger, Stuart Harris, Jordan Smith, Susan C. Tilton, William M. Baird, David E. Williams
FOOD AND CHEMICAL TOXICOLOGY
(2018)
Article
Instruments & Instrumentation
Alan J. Hidy, Thomas A. Brown, Scott J. Tumey, Susan R. H. Zimmerman, Narek Gharibyan, Matthias Frank, Graham Bench
NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS
(2018)
Article
Instruments & Instrumentation
T. J. Ognibene, K. W. Haack, G. Bench, K. W. Turteltaub
NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS
(2019)
Article
Chemistry, Inorganic & Nuclear
Narek Gharibyan, Ken Moody, Scott Tumey, Tom Brown, Pat Grant, Graham Bench, Dawn Shaughnessy
Article
Engineering, Multidisciplinary
A. D. McCartt, T. J. Ognibene, G. Bench, K. W. Turteltaub
MEASUREMENT SCIENCE AND TECHNOLOGY
(2014)
Article
Instruments & Instrumentation
T. H. Joshi, S. Sangiorgio, V. Mozin, E. B. Norman, P. Sorensen, M. Foxe, G. Bench, A. Bernstein
NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS
(2014)
Article
Multidisciplinary Sciences
Heather A. Enright, Miranda J. S. Falso, Michael A. Malfatti, Victoria Lao, Edward A. Kuhn, Nicholas Hum, Yilan Shi, Ana Paula Sales, Kurt W. Haack, Kristen S. Kulp, Bruce A. Buchholz, Gabriela G. Loots, Graham Bench, Kenneth W. Turteltaub
Article
Geochemistry & Geophysics
S. Szidat, G. Bench, V. Bernardoni, G. Calzolai, C. I. Czimczik, L. Derendorp, U. Dusek, K. Elder, M. E. Fedi, J. Genberg, O. Gustafsson, E. Kirillova, M. Kondo, A. P. McNichol, N. Perron, G. M. Santos, K. Stenstrom, E. Swietlicki, M. Uchida, R. Vecchi, L. Wacker, Y. L. Zhang, A. S. H. Prevot
Article
Instruments & Instrumentation
Taylor A. B. Broek, Ted J. Ognibene, Karis J. McFarlane, Kimber C. Moreland, Thomas A. Brown, Graham Bench
Summary: This study repurposed and optimized a 1 MV biomedical accelerator mass spectrometer for radiocarbon measurement of environmental samples, showing that the system can measure small samples with high precision and accuracy without the need for specific correction protocols.
NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS
(2021)
Review
Environmental Sciences
Michael A. Malfatti, Bruce A. Buchholz, Heather A. Enright, Benjamin J. Stewart, Ted J. Ognibene, A. Daniel McCartt, Gabriela G. Loots, Maike Zimmermann, Tiffany M. Scharadin, George D. Cimino, Brian A. Jonas, Chong-Xian Pan, Graham Bench, Paul T. Henderson, Kenneth W. Turteltaub