Article
Pharmacology & Pharmacy
Anne Mette Handler, Gitte Pommergaard Pedersen, Kim Troensegaard Nielsen, Christian Janfelt, Anders Just Pedersen, Malcolm R. Clench
Summary: In this study, HPLC-MS/MS and MALDI-MSI were used to analyze drug penetration in the skin, revealing the impact of different formulations on drug release and retention in the skin, with C1 showing the highest retention of tofacitinib and the gel and C2 demonstrating higher permeation.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2021)
Article
Chemistry, Analytical
Bingbing Liu, Xuanlin Meng, Kun Li, Jinggong Guo, Zongwei Cai
Summary: This study used MALDI-MSI to investigate the spatial distribution of lipids in cottonseeds, revealing heterogeneous distribution patterns of different lipid species within the seeds. The roles of these lipids in metabolic pathways were analyzed, and the relationship between their spatial distribution and functions was studied. The unique distribution patterns of lipid metabolites revealed by MSI may offer new insights into the spatial compartmentation of lipid metabolism in plants and can potentially aid genetic engineering efforts when combined with transcriptomics and proteomics.
Article
Chemistry, Multidisciplinary
Qiuqin Zhou, Stefano Rizzo, Janina Oetjen, Annabelle Fueloep, Miriam Rittner, Hartmut Gillandt, Carsten Hopf
Summary: Insufficient vacuum stability of matrix chemicals is a major limitation in MALDI mass spectrometry imaging of large tissue sample cohorts. A photo-cleavable caged molecule, DMNB-2,5-DHAP, was designed and synthesized for lipid MALDI-MSI of mouse brain tissue sections. DMNB-2,5-DHAP exhibited vacuum stability in a high vacuum MALDI ion source and its uncaging process resulted in the generation of 2,5-DHAP with the assistance of the built-in laser in the MALDI ion source.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Medicinal
Ariful Islam, Takumi Sakamoto, Qing Zhai, Md Muedur Rahman, Md Al Mamun, Yutaka Takahashi, Tomoaki Kahyo, Mitsutoshi Setou
Summary: This study applied three different tools of mass spectrometry imaging (MSI) to visualize drugs and their metabolites in mice. Among the three MSI tools, iMScope QT demonstrated better detection capability and higher speed for rapid mapping of targeted drugs. The study found that imipramine and its metabolites predominantly accumulated in the renal cortex of mice, while chloroquine and its metabolites accumulated highly in the renal pelvis and renal medulla. In addition, different accumulation patterns were observed in various regions of the mouse brain. These findings contribute to the understanding of the clinical properties, efficacy, and potential side effects of these drugs, and demonstrate the potential of iMScope QT for rapid mapping of small drugs and their metabolites in biological samples.
Article
Agriculture, Multidisciplinary
Chizumi Abe, Ye Zhang, Kazuhiro Takao, Kuni Sasaki, Koji Ochiai, Toshiro Matsui
Summary: This study visually investigated the dynamic production of soybean phytoalexins glyceollins in germinating soybeans inoculated with Aspergillus oryzae using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) imaging. The results showed that glyceollins were only produced in the seed coat and germinated root regions of soybeans, and inoculation of inactivated fungi also promoted glyceollin production, suggesting a possible association with the fungus body.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Christoph Bookmeyer, Ulrich Roehling, Klaus Dreisewerd, Jens Soltwisch
Summary: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a rapidly growing method in the life sciences. The study introduces a novel post-ionization scheme based on single-photon induced chemical ionization using pulsed RF-Kr lamps, which effectively improves the ionization efficiency for analytes. Through the interplay with chemical dopants, the signal of various lipid molecules from mammalian tissue sections was significantly boosted.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Pharmacology & Pharmacy
Eloy Pena-Rodriguez, Teresa Garcia-Berrocoso, Ezequiel Vazquez Fernandez, Francisco J. Otero-Espinar, Joaquin Abian, Francisco Fenandez-Campos
Summary: Confocal Raman microscopy and MALDI-TOF mass spectrometry imaging are two promising techniques for ex vivo skin imaging and quantification. The biodistribution of dexamethasone (DEX) loaded lipomers in the skin was compared using both techniques, with benzalkonium chloride (BAK) as a tracer. Confocal Raman microscopy showed higher DEX measurements, while MALDI-TOF MSI was more suitable for tracing BAK. Confocal Raman microscopy provided higher spatial resolution and observed specific skin structures, while MALDI-TOF MSI had a faster sampling rate and analyzed larger tissue areas.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Engineering, Environmental
Yanfang Zhao, Rabah Boukherroub, Lu Liu, Huizhi Li, Ru-Song Zhao, Qin Wei, Xiang Yu, Xiangfeng Chen
Summary: This study demonstrates the effective utilization of boron nitride quantum dots as an inorganic matrix in MALDI-MS & MSI for BPA analysis. The method allows non-destructive and in situ mapping of BPA in environmental water and plastic products, and detects BPA at nanomolar level.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Food Science & Technology
Daria Kucheriavaia, Dusan Velickovic, Nicholas Peraino, Apurva Lad, David J. Kennedy, Steven T. Haller, Judy A. Westrick, Dragan Isailovic
Summary: This study investigated the distribution of MC-LR in liver tissues of mice using MALDI-FT-ICR-MS imaging techniques. The results show that high-resolution MALDI-MS imaging can monitor the distribution of free microcystins in affected organs.
Article
Plant Sciences
Wenxiang Fan, Yuangui Yang, Linnan Li, Linhong Fan, Zhengtao Wang, Li Yang
Summary: This study comprehensively revealed the chemical diversity and dynamic transformation pattern of Panax notoginseng during processing, providing insights into the distinct bioactivities between raw and processed Panax notoginseng. The proposed approach is valuable for quality evaluation and control of natural product processing.
Article
Biochemical Research Methods
Raphael Legouffe, Olivier Jeanneton, Mathieu Gaudin, Aurore Tomezyk, Amandine Gerstenberg, Marc Dumas, Catherine Heusele, David Bonnel, Jonathan Stauber, Sylvianne Schnebert
Summary: Hyaluronic acid plays a vital role in the skin, and its reduction with age has led to widespread use of HA-containing products in cosmetics and aesthetic procedures. A new analytical method using MALDI-MSI has been developed to quantify HA content in the skin and compare relative concentrations in different tissue areas. The technique has shown a significant increase in HA levels following topical application of HA formula, demonstrating its potential efficacy in combating signs of aging.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Oncology
Bangzhen Ma, Yang Zhang, Jiwei Ma, Xinguo Chen, Chenglong Sun, Chengkun Qin
Summary: In this study, metabolites and lipids in hepatocellular carcinoma were profiled using MALDI-MSI analysis. The results showed stepwise metabolic alterations and increasing metabolic heterogeneity during the development of hepatocellular carcinoma. Precise spatial profiling of metabolites and lipids in heterogeneous tumor tissues contributes to a better understanding of tumor metabolic reprogramming.
CANCER CELL INTERNATIONAL
(2023)
Review
Chemistry, Analytical
M. Sejalon-Cipolla, P. Bruyat, S. Bregant, C. Malgorn, L. Devel, G. Subra, S. Cantel
Summary: MALDI-MS is crucial for detecting and identifying molecules involved in biological pathways, but many biomarkers remain undetectable due to various limitations. To overcome these challenges, labeling strategies and activity-based probes have been developed. Matrix-free derivatization techniques are currently gaining attention.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Holly-May Lewis, Priyanka Gupta, Kyle D. G. Saunders, Shazneil Briones, Johanna von Gerichten, Paul A. Townsend, Eirini Velliou, Dany J. V. Beste, Olivier Cexus, Roger Webb, Melanie J. Bailey
Summary: This work presents a new approach for measuring drug levels and lipid fingerprints in individual living mammalian cells. The method combines nanocapillary sampling with liquid chromatography to improve precision in drug analysis. By optimizing the transfer of analytes from the sampling capillary, the researchers achieved efficient measurement of drug analytes in 30 living cells. They also discovered cell-to-cell heterogeneity in drug molecule uptake and identified lipid features associated with bedaquiline uptake.
Article
Neurosciences
Merve Karayel-Basar, Irep Uras, Irem Kiris, Ahmet Tarik Baykal
Summary: Huntington's disease is a neurodegenerative disease with no cure that impairs daily activities. This study used MALDI-MSI and LC-MS/MS to analyze protein changes in the brain tissue of an HD mouse model. Significant differences in protein levels were found in different stages of the disease, and the changes in Tau, EF2, HSP70, and NogoA proteins were validated.
EUROPEAN JOURNAL OF NEUROSCIENCE
(2023)
Article
Biochemical Research Methods
Ekta Patel, Malcolm R. Clench, Andy West, Peter S. Marshall, Nathan Marshall, Simona Francese
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2015)
Editorial Material
Biochemical Research Methods
Laura M. Cole, Malcolm R. Clench
PROTEOMICS CLINICAL APPLICATIONS
(2015)
Article
Biochemical Research Methods
Ekta Patel, Laura M. Cole, Robert Bradshaw, Afnan Batubara, Christopher A. Mitchell, Simona Francese, Malcolm R. Clench
Article
Chemistry, Analytical
Cristina Russo, Neil Brickelbank, Catherine Duckett, Steve Mellor, Stephen Rumbelow, Malcolm R. Clench
ANALYTICAL CHEMISTRY
(2018)
Article
Chemistry, Applied
E. E. L. Lewis, M. R. T. Barrett, L. Freeman-Parry, R. A. Bojar, M. R. Clench
INTERNATIONAL JOURNAL OF COSMETIC SCIENCE
(2018)
Article
Biochemical Research Methods
Bryn Flinders, Josie Morrell, Peter S. Marshall, Lisa E. Ranshaw, Malcolm R. Clench
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2015)
Article
Chemistry, Analytical
John G. Swales, James W. Tucker, Nicole Strittmatter, Anna Nilsson, Diego Cobice, Malcolm R. Clench, C. Logan Mackay, Per E. Andren, Zoltan Takats, Peter J. H. Webborn, Richard J. A. Goodwin
ANALYTICAL CHEMISTRY
(2014)
Article
Biochemistry & Molecular Biology
Karol Jelonek, Monika Pietrowska, Malgorzata Ros, Adam Zagdanski, Agnieszka Suchwalko, Joanna Polanska, Michal Marczyk, Tomasz Rutkowski, Krzysztof Skladowski, Malcolm R. Clench, Piotr Widlak
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2014)
Article
Biochemical Research Methods
Laura M. Cole, Joanne E. Bluff, Vikki A. Carolan, Martyn N. Paley, Gillian M. Tozer, Malcolm R. Clench
Article
Multidisciplinary Sciences
Eylan Yutuc, Roberto Angelini, Mark Baumert, Natalia Mast, Irina Pikuleva, Jillian Newton, Malcolm R. Clench, David O. F. Skibinski, Owain W. Howell, Yuqin Wang, William J. Griffiths
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Chemistry, Analytical
Lucy E. Flint, Gregory Hamm, Joseph D. Ready, Stephanie Ling, Catherine J. Duckett, Neil A. Cross, Laura M. Cole, David P. Smith, Richard J. A. Goodwin, Malcolm R. Clench
ANALYTICAL CHEMISTRY
(2020)
Article
Biochemical Research Methods
Oana M. Voloaca, Calum J. Greenhalgh, Laura M. Cole, Malcolm R. Clench, Amy J. Managh, Sarah L. Haywood-Small
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2020)
Article
Biochemical Research Methods
Bryn Flinders, Josie Morrell, Peter S. Marshall, Lisa E. Ranshaw, Ron M. A. Heeren, Malcolm R. Clench
Summary: A straightforward method to generate 3D MALDI-MS images of selected molecules in lung tissue was presented, providing a deeper understanding of the distribution of molecules related to different anatomical features throughout the entire organ.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2021)
Article
Chemistry, Analytical
Oana M. Voloaca, Malcolm R. Clench, Gunda Koellensperger, Laura M. Cole, Sarah L. Haywood-Small, Sarah Theiner
Summary: This study reports the use of laser ablation-inductively coupled plasma time-of-flight mass spectrometry (LA-ICP-TOFMS) to analyze human malignant pleural mesothelioma (MPM) samples at the cellular level for the first time. The technique allows for simultaneous detection of asbestos fibers, metallome monitoring, and biomarker identification with high spatial resolution. The results provide insights into the pathology and potential toxic elements associated with MPM.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Marie-Claude Djidja, Emmanuelle Claude, Peter Scriven, David W. Allen, Vikki A. Carolan, Malcolm R. Clench
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2017)