Article
Microbiology
Jianxun Wang, Ruolin Wang, Feng Kang, Xia Yan, Ling Sun, Nana Wang, Yufeng Gong, Xiaoning Gao, Lili Huang
Summary: The composition and diversity of root microbial community are influenced by plant genotypes and soil environment. Grafting rootstock types of apple tree have an impact on the composition and diversity of root microbial community, as well as the resistance of apple tree to apple Valsa canker. The influence of grafting rootstock types on the composition of the root fungal community is greater than that of bacteria.
Article
Plant Sciences
Yakupjan Haxim, Gulnaz Kahar, Xuechun Zhang, Yu Si, Abdul Waheed, Xiaojie Liu, Xuejing Wen, Xiaoshuang Li, Daoyuan Zhang
Summary: This study identified and analyzed the chitinase gene family in apple species and found that these genes may play a crucial role in the defense against fungal pathogens.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Ronghao Liu, Jianyu Li, Feiran Zhang, Dian Zheng, Yali Chang, Liangsheng Xu, Lili Huang
Summary: The study found that Bacillus velezensis strain D4, as a potential biocontrol agent, has strong inhibitory effects on apple Valsa canker and can promote plant growth to control the disease. The D4 strain is able to suppress the mycelial growth of the pathogen and promote plant growth by producing siderophores and solubilizing phosphorus.
BIOLOGICAL CONTROL
(2021)
Article
Biochemistry & Molecular Biology
Alice Nevone, Francesca Lattarulo, Monica Russo, Giada Panno, Paolo Milani, Marco Basset, Maria Antonietta Avanzini, Giampaolo Merlini, Giovanni Palladini, Mario Nuvolone
Summary: In the next-generation sequencing era, RT-qPCR remains widely used for quantifying nucleic acids due to its popularity, versatility, and cost-effectiveness. However, accurate measurement of transcriptional levels requires appropriate reference genes for normalization. Here, we present a strategy that utilizes publicly available transcriptomic datasets to select suitable reference genes for specific clinical or experimental settings and includes a pipeline for RT-qPCR assay design and validation. We demonstrated the efficacy of this strategy by identifying and validating reference genes for transcriptional studies of bone-marrow plasma cells in AL amyloidosis patients. This strategy can be applied to other settings with publicly available transcriptomic datasets.
Article
Environmental Sciences
Ju Mu, Yu Wang, Miao Wang, Dayi Zhang, Mingying Liu
Summary: This study constructed a mouse model exposed to poly-propylene microplastics (PP-MPs) to evaluate the bioaccumulation of PP-MPs in the kidney and explore potential reference genes using RT-qPCR. The stability and reliability of 19 candidate reference genes were assessed using four algorithms, and the top five most stable genes were determined (Cox4i1, Rps3, Rps9, Rps18, and Gapdh). Three genes associated with different biochemical processes were chosen to verify the identified reference genes, showing different expression profiles and emphasizing the importance of selecting reliable reference genes.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Plant Sciences
Xiang-Long Meng, Rui Yang, An-Tai Liu, Tong-le Hu, Ya-Nan Wang, Ke-Qiang Cao, Shu-Tong Wang
Summary: Exposure to lower temperatures between -10 degrees C and 10 degrees C increases the severity of apple tree disease, promoting pathogenic fungus growth and elevating the expression of pathogenic genes.
Article
Plant Sciences
Leandro Francisco de Oliveira, Amanda Rusiska Piovezani, Dimitre A. Ivanov, Leonardo Yoshida, Eny Iochevet Segal Floh, Massuo Jorge Kato
Summary: This study identified stable reference genes for gene expression analysis in four Piper species and provided a list of recommended gene pairs for each species. These findings will facilitate further transcriptomic and functional gene research.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Plant Sciences
Maria Noelia Ulrich, Esteban Muniz-Padilla, Alejandra Corach, Esteban Hopp, Daniela Tosto
Summary: This study evaluated the stability of different reference genes of glyphosate-resistant S. halepense, finding that PP2A and ARI8 were the most stable genes. The results provide valuable information for gene expression analysis under glyphosate stress and will facilitate resistance mechanism studies in this weed species.
Article
Agricultural Engineering
Rui Xue, Zhi-Zhen Chi, Xuan-Min Yang, Ling Qiu, Ming-Qiang Zhu
Summary: Wood tar obtained from pyrolysis of waste fruit wood was used as a biofungicide to control apple canker caused by V. mali. The study showed that wood tar concentrations were positively correlated with hypha inhibition rate and residual duration. Wood tar demonstrated promising prospects as a biological control agent against apple canker.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Plant Sciences
Qizhang Wang, Chunqian Guo, Shipeng Yang, Qiwen Zhong, Jie Tian
Summary: The aim of this study was to identify suitable reference genes for gene expression analysis during different growth conditions in garlic. Nine candidate reference genes were selected using garlic transcriptome sequence data and their expression levels were evaluated in specific tissues under drought and cold stress. Several statistical methods were used to assess the stability of the reference gene expression levels. The most stable reference genes were identified, and their reliability was confirmed by evaluating the expression of a stress-responsive gene. This study provides a theoretical reference for gene expression analysis in garlic under stress conditions.
Article
Plant Sciences
Pengliang Han, Ruotong Zhang, Rui Li, Fudong Li, Jiajun Nie, Ming Xu, Chengli Wang, Lili Huang
Summary: This study reveals that MdVQ12 enhances apple resistance to Valsa canker by regulating the expression of MdHDA19 and thereby regulating the JA and ET signaling pathways, offering potential candidate gene resources for breeding apple Valsa canker-resistant germplasm.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Chaofan Jiang, Lin Zhou, Qingchen Zhao, Mengke Wang, Sirui Shen, Te Zhao, Kaidi Cui, Leiming He
Summary: This study validated the expression stability of seven candidate reference genes in Sclerotium rolfsii under different conditions and recommended suitable reference gene combinations for RT-qPCR normalization. The results showed that using one or two reference genes to normalize the expression of target genes in S. rolfsii did not significantly differ.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Yi Wang, Zi-Wei Wu, Ting Fang, Yu-Qing Zhang, Lu Chen, Zhi-Qiang Du, Cai-Xia Yang
Summary: Identification of stable internal reference genes is important for accurate normalization and quantification of target transcripts. This study aimed to identify stable reference genes in porcine immature Sertoli cells (iSCs) after acute heat stress treatment. Rpl32 and Actb were found to be the most stable internal reference genes.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Plant Sciences
Yuanyuan Jing, Minghui Zhan, Chunrong Li, Tingting Pei, Qi Wang, Pengmin Li, Fengwang Ma, Changhai Liu
Summary: This study found that overexpression of the FERONIA gene compromised resistance against Valsa canker in apple trees. The compromised resistance was likely attributed to altered hormone levels, suppressed accumulation and activities of defense compounds, and disrupted hypersensitive reactions mediated by a specific protein.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Jianfei Chen, Ziwei Chen, Jilong Zhang, Wuchao Zhuang, Xiaotong Zheng
Summary: This study utilized different algorithms to evaluate the stability of internal reference genes in the gastrointestinal tract of chickens. The most suitable reference genes for each type of GI tissue were identified. Commonly used internal reference genes exhibited poor stability and are not recommended for future gene expression studies in the GI region.
Article
Plant Sciences
Gensheng Zhang, Wei Liu, Lin Wang, Meng Ju, Xiaxia Tian, Zhimin Du, Zhensheng Kang, Jie Zhao
Summary: Through analyzing the avirulence and virulence of 120 selfed progeny lines from Berberis shensiana, we found that the TSA-6 Puccinia striiformis f. sp. tritici (Pst) isolate is virulent against the Yr5 resistance gene and exhibited 73 different virulence phenotypes compared to the parental isolate. Further analysis revealed the genetic composition of the TSA-6 isolate at various resistance loci and identified quantitative trait loci associated with virulence at 20 Yr resistance loci. These findings contribute to understanding Pst virulence evolution and simplifying breeding for wheat cultivars with effective resistance to wheat stripe rust.
Article
Plant Sciences
Zhimin Du, Yuelin Peng, Gensheng Zhang, Li Chen, Shuchang Jiang, Zhensheng Kang, Jie Zhao
Summary: This study revealed that the wheat stripe rust pathogen can undergo sexual reproduction on susceptible barberry in late autumn in Tibet, China. The findings contribute to the understanding of wheat stripe rust and provide insights into disease management in the region.
Article
Biochemistry & Molecular Biology
Jinping Wei, Xiaodong Wang, Zeyu Hu, Xiaojie Wang, Jialiu Wang, Jianfeng Wang, Xueling Huang, Zhensheng Kang, Chunlei Tang
Summary: The obligate biotrophic fungus Puccinia striiformis f. sp. tritici (Pst) employs the effector Hasp98 to inhibit wheat's immune responses and promote the infection of P. striiformis. Hasp98 interacts with the wheat protein TaMAPK4, inhibiting its kinase activity and interfering with the MAPK signaling pathway. This study provides insights into how Pst effectors regulate host defense responses and promote stripe rust disease.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Plant Sciences
Linying Du, Xueling Huang, Li Ding, Zhongxue Wang, Dongling Tang, Bin Chen, Lanjiya Ao, Yuling Liu, Zhensheng Kang, Hude Mao
Summary: This study reveals the important role of TaERF87 in regulating drought tolerance in wheat. TaERF87 enhances drought tolerance by activating the expression of proline biosynthesis genes and synergistically interacts with TaAKS1. These findings contribute to the development of elite drought-tolerant wheat varieties.
Article
Biochemistry & Molecular Biology
Xiaobo Wei, Xueling Huang, Weiling Yang, Xinran Wang, Tao Guan, Zhensheng Kang, Jie Liu
Summary: This study cloned and identified a wheat G6DPH gene, TaG6PDH2, which is significantly upregulated in wheat leaves infected with Puccinia striiformis f. sp. tritici (Pst) or treated with abiotic stress factors. Functional analysis revealed that TaG6PDH2 encodes a chloroplast-targeting G6PDH protein. Silencing TaG6PDH2 compromised wheat resistance to Pst avirulent pathotype CYR23, and TaG6PDH2 was found to interact with wheat glutaredoxin TaGrxS4.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yingchao Sun, Xiaojie Wang, Feiyang Liu, Haoyu Guo, Jianfeng Wang, Zetong Wei, Zhensheng Kang, Chunlei Tang
Summary: Plant cell surface RLKs play a crucial role in recognizing and responding to pathogen invasion. In this study, a specific RLK gene (TaBIR1) was found to be upregulated during wheat resistance to Puccinia striiformis f. sp. tritici (Pst) infection. TaBIR1 was shown to be located on the plasma membrane and its knockdown resulted in decreased wheat resistance to stripe rust. Overexpression of TaBIR1 triggered constitutive immune responses in Nicotiana benthamiana. Moreover, TaBIR1's immune response is dependent on the homologues of NbBAK1 in wheat.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Wen Song, Zhiyuan Yin, Xinyu Lu, Danyu Shen, Daolong Dou
Summary: This study found that three plant secondary metabolites (citral, carvacrol, and trans-2-decenal) exhibited strong antimicrobial effects against Phytophthora capsici. Citral at a low concentration restricted P. capsici infection in Nicotiana benthamiana but had no effect on mycelial growth. At a high concentration, citral affected mycelial growth, spore germination, and cell membrane permeability. Additionally, citral inhibited the expression of virulence-related genes in P. capsici and regulated the expression of effector genes during the infection process.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Nan Wang, Zhiyuan Yin, Yingke Wu, Jishuo Yang, Yaning Zhao, Paul Daly, Yong Pei, Dongmei Zhou, Daolong Dou, Lihui Wei
Summary: The oomycete Pythium myriotylum is a necrotrophic pathogen that induces cell death in plants. A small cysteine-rich protein, PmSCR1, was identified and found to trigger immune responses in host plants. PmSCR1 is independent of enzymatic activity and its elicitor function has been shown to promote resistance to Phytophthora sojae and Phytophthora capsici infection.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2023)
Article
Plant Sciences
Fan Ji, Aihong Zhou, Bofan Liu, Yue Liu, Yaoxuan Feng, Xiaodong Wang, Lili Huang, Zhensheng Kang, Gangming Zhan
Summary: This study assessed the resistance risk of wheat leaf rust to the fungicide triadimefon in China. The majority of tested Pt isolates were sensitive to triadimefon, but 10.2% exhibited varying degrees of resistance. The resistant isolates showed strong adaptive traits in parasitic fitness. No correlation was found between triadimefon and other fungicides with similar or different modes of action. The risk of resistance to triadimefon in Pt may be low to moderate.
Article
Virology
Yanhui Zhang, Hualong Guo, Siyu Zhou, Daipeng Chen, Gang Xu, Zhensheng Kang, Li Zheng
Summary: We report a new mitovirus, named Puccinia striiformis mitovirus 2 (PsMV2), from the wheat stripe rust fungus. PsMV2 exhibited high multiplication during Pst infection and suppressed programmed cell death triggered by Bax. Silencing of PsMV2 reduced fungal growth and pathogenicity of Pst. Our results indicate that PsMV2 promotes pathogenicity and has a wide distribution in field isolates of Pst.
Article
Plant Sciences
Lei Pi, Yifan Zhang, Jinghao Wang, Nan Wang, Zhiyuan Yin, Daolong Dou
Summary: In this study, a G-type lectin RLK named NbERK1 was found to enhance plant resistance to the fungal pathogen Sclerotinia sclerotiorum through regulating chitin signaling. The LysM-RLK NbCERK1/NbLYK4 was also shown to positively mediate plant resistance to S. sclerotiorum. These findings highlight the importance of NbERK1 in chitin perception and signaling for plant resistance to fungal pathogens.
PHYTOPATHOLOGY RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Jia Guo, Ying Mou, Yuanxing Li, Qing Yang, Xue Wang, Haocheng Lin, Zhensheng Kang, Jun Guo
Summary: This study identified 131 chitinase genes in 10 fungal genomes and found 16 chitinase genes from Puccinia striiformis f. sp. tritici (Pst). The expression of PstChia1 was the highest during Pst infection and it showed chitinase enzyme activity. Silencing PstChia1 limited the growth and reduced the virulence of Pst, indicating its role in pathogenicity and interference with plant immunity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Muye Xiao, Dezhi Chen, Saifei Liu, Anle Chen, Anfei Fang, Binnian Tian, Yang Yu, Chaowei Bi, Zhensheng Kang, Yuheng Yang
Summary: A chitin deacetylase called PsCDA2 was identified in the wheat stripe rust fungus Pst, which inhibits plant cell death and protects the fungus from host chitinase. This discovery provides insights into the mechanism of plant immune suppression and has implications for wheat stripe rust control.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Wanlu Duan, Zhenkai Hao, Huihui Pang, Yuxi Peng, Yiwen Xu, Yanfei Zhang, Ying Zhang, Zhensheng Kang, Jing Zhao
Summary: Regulation of host gene expression by fungal pathogens is an important strategy for promoting disease. In this study, a fungal effector called PstGTA1 was identified from Puccinia striiformis f. sp. tritici (Pst). It was found to have transcriptional activating activity and contribute to the virulence of Pst by targeting the promoter of a wheat gene called TaSIG. This study provides the first evidence for a fungal effector modulating host gene transcription through promoter binding and histone acetylation.
Article
Biochemistry & Molecular Biology
Zhiyuan Yin, Danyu Shen, Yaning Zhao, Hao Peng, Jinding Liu, Daolong Dou
Summary: Transmembrane kinases (TMKs) play important roles in cellular signaling. The kinase domains of most metazoan and plant TMKs belong to the S/T/Y-kinase superfamily. TMKs in protists have diverse origins and functional diversity. Despite belonging to different kinase subfamilies, protist LRR and GPCR-type TMKs share similar sensory domain architectures with plant and animal TMKs.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
