期刊
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
卷 29, 期 3, 页码 549-557出版社
SPRINGER
DOI: 10.1007/s11274-012-1209-9
关键词
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资金
- Shanghai National Science Foundation [11ZR14324000]
- Development Fund of Shanghai Academy of Agricultural Sciences [2011-10]
- Key Project Fund of the Shanghai Municipal Committee of Agriculture [2009-6-4, 2011-1-8]
- International Scientific and Technological Cooperation [2010DFA 62320, 11230705900]
- National Natural Science Foundation [31071486]
A novel aroA gene encoding 5-enolpyruvylshikimate-3-phosphate synthase from Bacillus cereus was identified and overexpressed by genomic library construction and complementary screening. The enzyme was then purified to homogeneity. We also transformed the aroA (B. cereus) gene into Arabidopsis thaliana by a floral dip method, and demonstrated that transgenic A. thaliana plants exhibited significant glyphosate resistance compared with the wild type. These results strongly suggested that the strategy was highly efficient and advantageous for rapidly cloning aroA genes from microorganisms in natural environments.
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