期刊
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
卷 26, 期 5, 页码 945-950出版社
SPRINGER
DOI: 10.1007/s11274-009-0244-7
关键词
Bacillus subtilis; Chitinase; Fusarium; Shrimp and crab shell
资金
- National Science Council, Republic of China [NSC97-2221-E346-005]
The production of inexpensive chitinolytic enzymes is an element in the utilization of shellfish-processing waste. In this study, shrimp and crab shell powder, prepared by treating shrimp- and crab-processing waste by boiling and crushing, was used as a substrate for the isolation of an antifungal chitinase-producing microorganism. Bacillus subtilis NPU 001, a strain isolated from soil samples, excreted a chitinase when cultured in a medium containing 2% (w/v) shrimp and crab shell powder as the major carbon source. The chitinase, which was purified by sequential chromatography, had a Mw of 31 kDa and a pI of 5.4. The purified chitinase (2 mg ml(-1)) inhibited hyphal extension of the fungus Fusarium oxysporum. Compared with other known bacterial chitinases, the unique characteristics of NPU 001 chitinase include antifungal activity against plant-pathogenic fungi and the production of chitotriose as the major enzymatic hydrolysate from colloidal chitin.
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