Article
Agriculture, Multidisciplinary
Qingru Li, Jing Zhang, Xiaoyu Guan, Yanhong Lu, Yankai Liu, Jiahuan Liu, Na Xu, Chunyu Cai, Bo Nan, Xia Li, Jingsheng Liu, Yuhua Wang
Summary: This study found that the addition of soybean oil can promote the accumulation of astaxanthin in Phaffia rhodozyma by increasing the content of astaxanthin esters.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Agricultural Engineering
Jing Zhang, Qingru Li, Yanhong Lu, Xiaoyu Guan, Jiahuan Liu, Na Xu, Chunyu Cai, Xia Li, Bo Nan, Jingsheng Liu, Yuhua Wang
Summary: The study demonstrated that titanium dioxide (TiO2) stress significantly increased the astaxanthin yield of Phaffia rhodozyma PR106 by accelerating glucose uptake, promoting ethanol reuse, and shifting carbon flux to the astaxanthin synthesis pathway. This metabolic regulation under TiO2 stress improved the production of astaxanthin in PR106 strain, while not directly related to mRNA transcription and post-translational modifications regulation. Additionally, the regulation of ROS metabolism continued in the PR106 strain.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Li-Li Miao, Shuang Chi, Ting-Ting Hou, Zhi-Pei Liu, Ying Li
Summary: The inhibition of cell growth in P. rhodozyma at temperatures >21 degrees C is due to blocking of DNA, RNA, fatty acid, and cell wall biosynthesis processes. Through metabolic engineering, enhancing the astaxanthin synthesis pathway can improve the adaptability of P. rhodozyma to high temperatures, ultimately increasing astaxanthin production.
MICROBIAL CELL FACTORIES
(2021)
Article
Biochemistry & Molecular Biology
Zhipeng Li, Lina Chen, Haoyi Yang, Tianli Li, Xiping Du, Ning He, Zedong Jiang, Lijun Li, Hui Ni
Summary: Phaffia rhodozyma is a potential industrial source of natural astaxanthin. This study analyzed the relationship between two pathways (dicyclic and monocyclic) and astaxanthin production in P. rhodozyma. Knockout of CRTYB gene resulted in undetectable astaxanthin levels, indicating its importance in both pathways. Knockout of CRTS gene promoted the monocyclic pathway and increased astaxanthin production.
PROCESS BIOCHEMISTRY
(2022)
Review
Food Science & Technology
Cassamo U. Mussagy, Jorge F. B. Pereira, Laurent Dufosse, Vijaya Raghavan, Valeria C. Santos-Ebinuma, Adalberto Pessoa Jr
Summary: This article reviews the main methods for producing Astaxanthin (AXT) using the yeast Phaffia rhodozyma strains, emphasizing the impact of conventional and non-conventional procedures for extracting AXT from yeast cells. Furthermore, future research directions are identified, including the use of low-cost residues to improve the economic and environmental sustainability of the bioprocess, the use of environmentally-friendly and low-energy operations for the extraction and purification of AXT, as well as the need for further human clinical trials using yeast-based AXT.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Agricultural Engineering
Wenshao Li, Carlos H. Luna-Flores, Raveendra Anangi, Renwu Zhou, Xinle Tan, Marius Jessen, Lian Liu, Rusen Zhou, Tianqi Zhang, Alexander Gissibl, Patrick J. Cullen, Kostya (Ken) Ostrikov, Robert E. Speight
Summary: In this study, a bubble discharge reactor was developed to generate plasma-activated water (PAW) to produce PAW-made yeast malt (YM) medium. Strains cultured in 15 and 30 min-treated PAW-made medium produced significantly higher amounts of carotenoids and astaxanthin due to oxidative stress induced by PAW. This study demonstrates the potential of PAW to increase astaxanthin yields for industrial-scale production.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Zhipeng Li, Haoyi Yang, Chenhua Zheng, Xiping Du, Hui Ni, Ning He, Liang Yang, Li You, Yanbing Zhu, Lijun Li
Summary: Metabolic regulation can enhance astaxanthin production in Phaffia rhodozyma. The optimal combination of regulators improves astaxanthin cell yield by simultaneously promoting the main pathway and inhibiting competing pathways.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Microbiology
Zhihui Shi, Xiaoxian He, Hailiang Zhang, Xuena Guo, Yanfei Cheng, Xuelian Liu, Zhaoyue Wang, Xiuping He
Summary: In this study, atmospheric and room temperature plasma mutagenesis technology was used to genetically improve Phaffia rhodozyma. The key gene GST1 affecting carotenoid synthesis was discovered through comparative genomics and transcriptomics analysis. This research is important for increasing pigment content and improving the growth cycle and fermentation temperature of P. rhodozyma.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Engineering, Environmental
Cassamo U. Mussagy, Amanda dos Santos, Gabriely F. G. Militao, Jessica Carolina Silva de Oliveira, Gisela de A. Umbuzeiro, Guilherme Peixoto, Adalberto Pessoa Jr, Valeria C. Santos-Ebinuma
Summary: This study uses the yeast Phaffia rhodozyma as biomass feedstock to produce astaxanthin and generate biogas from waste biomass, aiming to achieve sustainability and minimize the use of synthetic counterparts.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Carlos H. Luna-Flores, Alexander Wang, Juhani von Hellens, Robert E. Speight
Summary: This study found that mutant strains of P. rhodozyma generated through mutagenesis showed increased production of astaxanthin (AX). Culture media optimization and kinetic modeling were used to maximize AX production. Genomic variant analysis revealed important mutations in protein coding regions associated with regulators and catalysts of AX production.
JOURNAL OF BIOTECHNOLOGY
(2022)
Article
Engineering, Chemical
Cassamo U. Mussagy, Fabiane O. Farias, Niura M. Bila, Maria J. S. M. Giannini, Jorge F. B. Pereira, Valeria C. Santos-Ebinuma, Adalberto Pessoa Jr
Summary: This study proposes a biocompatible method using ionic liquids (ILs) for the selective recovery of beta-carotene and astaxanthin from Phaffia rhodozyma cells. The most efficient IL solution was identified and an integrated platform for solvent recycling and biomolecule recovery was developed. The results demonstrate the efficacy of ILs for one-step extraction of beta-carotene and astaxanthin from microbial-based biomass.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Jianping Jia, Feifei Li, Yifei Luan, Siru Liu, Zhitao Chen, Guoliang Bao
Summary: This study found that the addition of exogenous salicylic acid (SA) significantly increased astaxanthin production in Phaffia rhodozyma. Transcriptomic analysis revealed the impact of SA on gene expression. It was also discovered that overexpression of the polyamine transporter gene (PT) further improved astaxanthin yield.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Agricultural Engineering
Jing Zhang, Xiaoyu Guan, Yanhong Lu, Yankai Liu, Na Xu, Chunyu Cai, Qingru Li, Jiahuan Liu, Yuhua Wang, Jingsheng Liu
Summary: Carotenoid is widely used in food, medicine, and feed industries. The whole genome sequencing of P. rhodozyma PR106 strain revealed that carotenoid production increased under titanium dioxide stress, and the production of fatty acids also increased.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Cassamo U. Mussagy, Daniela Remonatto, Flavio P. Picheli, Ariela V. Paula, Rondinelli D. Herculano, Valeria C. Santos-Ebinuma, Renan L. Farias, Bruno S. D. Onishi, Sidney J. L. Ribeiro, Jorge F. B. Pereira, Adalberto Pessoa
Summary: A process using bio-based solvents was developed for the fractionation and recovery of compounds from red yeast, achieving efficient separation and recovery of astaxanthin, beta-carotene, proteins, and lipids. Furthermore, biologically active bioplastics were produced by mixing carotenoid-rich extracts with bacterial cellulose.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Cassamo U. Mussagy, Fabiane O. Farias, Valeria C. Santos-Ebinuma, Jorge F. B. Pereira, Adalberto Pessoa Jr
Summary: Microbial-based pigments, such as astaxanthin and β-carotene produced by P. rhodozyma, have attracted attention as greener and more effective alternatives in the cleaning and cosmeceutical industries. However, the optimization of downstream processing operations for carotenoid recovery from P. rhodozyma has not been fully achieved. This study proposes a sustainable one-pot process using biocompatible and renewable solvents for the recovery of astaxanthin and β-carotene from P. rhodozyma biomass and formulation of bioactive soaps.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2023)
Article
Chemistry, Applied
Huijing Chen, Qiong Xiao, Huifen Weng, Yonghui Zhang, Qiuming Yang, Anfeng Xiao
CARBOHYDRATE POLYMERS
(2020)
Article
Chemistry, Medicinal
Yong-Hui Zhang, Yuan Shao, Chao Jiao, Qiu-Ming Yang, Hui-Fen Weng, An-Feng Xiao
Article
Biochemistry & Molecular Biology
Yuan Shao, Yong-Hui Zhang, Feng Zhang, Qiu-Ming Yang, Hui-Fen Weng, Qiong Xiao, An-Feng Xiao
Article
Chemistry, Applied
Cong Zhang, Ding An, Qiong Xiao, Huifen Weng, Yonghui Zhang, Qiuming Yang, Anfeng Xiao
FOOD HYDROCOLLOIDS
(2020)
Article
Biochemistry & Molecular Biology
Yong-Hui Zhang, Yi Ru, Chao Jiang, Qiu-Ming Yang, Hui-Fen Weng, An-Feng Xiao
PROCESS BIOCHEMISTRY
(2020)
Article
Chemistry, Applied
Huijing Chen, Fuquan Chen, Qiong Xiao, Menghao Cai, Qiuming Yang, Huifen Weng, Anfeng Xiao
Summary: The novel amphiphilic agar modified with octenyl succinic anhydride (OSAR) showed higher transparency and freeze-thaw stability, as well as improved emulsion stability and oil loading capacity compared to native agar (NA). With DS values of 0.06 and 0.12, OSAR exhibited increased gel transparency and decreased syneresis, lower interface tension and average molecular weight, decreased thermal stability, enhanced gel springiness, more liquid-like properties with low gel strength, and lower turbidity and melting temperatures than NA.
CARBOHYDRATE POLYMERS
(2021)
Article
Chemistry, Applied
Ding An, Qiong Xiao, Cong Zhang, Menghao Cai, Yonghui Zhang, Huifen Weng, Fuquan Chen, Anfeng Xiao
Summary: The study demonstrates that bleaching with hydrogen peroxide can reduce pigment content in agar, increase whiteness, and improve transparency and quality characteristics. High-whiteness agar has lower sulfate and ash content, higher transparency, better thermal stability, and water-holding capacity. It is superior to raw agar in terms of jelly preparation and microbial counting.
FOOD HYDROCOLLOIDS
(2021)
Article
Chemistry, Medicinal
Chi Wang, Pei-Xu Chen, Qiong Xiao, Qiu-Ming Yang, Hui-Fen Weng, Yong-Hui Zhang, An-Feng Xiao
Summary: A non-toxic chitosan-based carrier was successfully constructed for tannase immobilization, achieving better environmental adaptability and stability with enhanced biological activities of tea extract through optimized activation and immobilization conditions.
Article
Chemistry, Medicinal
Cong Zhang, Ding An, Qiong Xiao, Fu-Quan Chen, Yong-Hui Zhang, Hui-Fen Weng, An-Feng Xiao
Summary: Agarose, a natural seaweed polysaccharide widely used in medicine, food, and biological fields, was explored in this study for an eco-friendly method of preparation. After desulfation with H2O2, the agarose can be used as a gel electrophoresis medium, showing removal of sulfur from the agar molecules in the form of sulfate ions and metal sulfate.
Article
Chemistry, Medicinal
Qiong Xiao, Xinyi Wang, Jiabin Zhang, Yonghui Zhang, Jun Chen, Fuquan Chen, Anfeng Xiao
Summary: Optimizing alkali treatment process alone is not sufficient to achieve the best agar yield and quality. Changes in algae morphology and weight, as well as the physicochemical properties of agar, need to be monitored and analyzed comprehensively during pretreatment processes. Acidification can improve yield by solving the problem of algae hardening after alkali treatment, and enzyme-assisted extraction technology shows potential to replace traditional alkali-extraction technology by promoting desulfurization efficiency and gel strength.
Article
Chemistry, Medicinal
Feng Jiang, Yao Liu, Qiong Xiao, Fuquan Chen, Huifen Weng, Jun Chen, Yonghui Zhang, Anfeng Xiao
Summary: An eco-friendly method for extracting iota-carrageenan from seaweed was developed using boiling and a low concentration of Ca(OH)(2). Compared to the traditional method using NaOH, this method resulted in a higher yield and gel strength, shorter extraction time, and reduced alkali and water usage. The extracted iota-carrageenan showed similar structures and thermal stability, but different textural and rheological properties compared to NaOH-extracted carrageenan.
Review
Chemistry, Medicinal
Feng Jiang, Xin-Wei Xu, Fu-Quan Chen, Hui-Fen Weng, Jun Chen, Yi Ru, Qiong Xiao, An-Feng Xiao
Summary: Numerous compounds in the ocean are contributing to the development of the biomedical field. Agarose, derived from marine red algae, is a polysaccharide with temperature-sensitive gelling behavior, excellent mechanical properties, and high biological activity, making it important in biomedical applications. However, its single structural composition limits its adaptability to complex biological environments. This review examines the preparation, modification, and biomedical applications of agarose, focusing on its potential in isolation and purification, wound dressings, drug delivery, tissue engineering, and 3D printing.
Article
Chemistry, Applied
Luyao Zhang, Qiong Xiao, Yonghui Zhang, Huifen Weng, Siyuan Wang, Fuquan Chen, Anfeng Xiao
Summary: Agar was chemically modified with C12-C18 anhydrides to investigate its physicochemical and emulsifying properties. The gel properties of the esterified agar decreased as the anhydrides' substitution degree and carbon chain length increased. Esterified agar with long hydrophobic chains and carboxyl groups exhibited good surface activity and high negative charge. Increasing the anhydrides' substitution degree and carbon chain length enhanced the emulsifying properties, resulting in small particle size and high emulsion stability. The study provides insights into the structural and amphiphilic properties of agar-based emulsions stabilized by anhydride-modified agars.
FOOD HYDROCOLLOIDS
(2023)
Article
Biochemistry & Molecular Biology
Qiong Xiao, Guo Chen, Yonghui Zhang, Huifen Weng, Menghao Cai, Anfeng Xiao
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
Correction
Chemistry, Applied
Qiong Xiao, Huifeng Weng, Guo Chen, Anfeng Xiao
