Article
Cell Biology
Md Mahfuz Al Mamun, Muhammad Riaz Khan, Yifu Zhu, Yuwei Zhang, Shuai Zhou, Ran Xu, Ihtisham Bukhari, Rick F. Thorne, Jinming Li, Xu Dong Zhang, Guangzhi Liu, Song Chen, Mian Wu, Xiaoyuan Song
Summary: This study identifies the E3 ubiquitin ligase Stub1 as a negative regulator of pluripotency in embryonic stem cells (ESCs). Stub1 catalyzes the degradation of Sox2, Oct4, and Nanog proteins, thereby reducing pluripotency. Deficiency of Stub1 enhances somatic cell reprogramming and delays differentiation, while its overexpression triggers ESC differentiation.
Article
Cell Biology
Jerry Hung-Hao Lo, Miguel Edwards, Justin Langerman, Rupa Sridharan, Kathrin Plath, Stephen T. Smale
Summary: By examining dynamic ranges of gene expression, the authors found that Oct4 and Sox2 binding is enriched near genes with large dynamic ranges of expression. Their results suggest that Oct4 and Sox2 directly establish both active and silent transcriptional states in pluripotent cells at a large number of genes subject to dynamic regulation.
GENES & DEVELOPMENT
(2022)
Article
Oncology
Mikella Robinson, Samuel F. Gilbert, Jennifer A. Waters, Omar Lujano-Olazaba, Jacqueline Lara, Logan J. Alexander, Samuel E. Green, Gregory A. Burkeen, Omid Patrus, Zinia Sarwar, Ryne Holmberg, Christine Wang, Carrie D. House
Summary: The findings suggest that SOX2 may be a more consistent indicator of ovarian tumor initiating cells (TICs) that contribute to tumor repopulation following chemotherapy. Further studies evaluating SOX2 in TIC biology will enhance understanding of mechanisms driving ovarian cancer relapse.
Review
Cell Biology
Puja Agrawal, Sridhar Rao
Summary: Cell fate decisions rely on gene expression changes mediated by regulatory elements like enhancers and CTCF binding sites. These elements play crucial roles in early mammalian development and impact cell-type specific gene expression.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Arun Kumar Burramsetty, Ken Nishimura, Takumi Kishimoto, Muhammad Hamzah, Akihiro Kuno, Aya Fukuda, Koji Hisatake
Summary: This study identified and characterized proteins associated with the Nanog promoter in mouse embryonic stem cells (mESCs) using unbiased methods. The results revealed the importance of these proteins, including RNA-binding proteins (RBPs), in the maintenance of pluripotency and the reprogramming of somatic cells. The findings highlight the functional relevance of RBPs in ESC differentiation and somatic cell reprogramming.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Xuepeng Wang, Chengcheng Song, Ying Ye, Yashi Gu, Xuemei Li, Peixin Chen, Dongliang Leng, Jing Xiao, Hao Wu, Sisi Xie, Weiwei Liu, Qi Zhao, Di Chen, Xi Chen, Qiang Wu, Guokai Chen, Wensheng Zhang
Summary: BRD9 plays a crucial role in the regulation of self-renewal and differentiation of human embryonic stem cells, and is involved in the progression of pancreatic and breast cancers.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Chemistry, Medicinal
Nattamon Hongwiangchan, Nicharat Sriratanasak, Duangdao Wichadakul, Nithikoon Aksorn, Supakarn Chamni, Pithi Chanvorachote
Summary: CIN-RM has been shown to suppress lung cancer stem cells by inhibiting their colony and tumor spheroid formation. It also affects central survival and stem cell regulatory proteins, leading to the depletion of CSC markers. The compound's mechanism of action involves inhibiting Akt/mTOR signaling and promoting c-Myc degradation.
Article
Biochemistry & Molecular Biology
Peng Ye, Xiaoxia Chi, Xiuwen Yan, Fangqin Wu, Zhigang Liang, Wen-Hao Yang
Summary: This study reveals the crucial role of alanine-glyoxylate aminotransferase (AGXT) in supporting liver cancer stem cells (LCSCs) and maintaining their stemness. AGXT may sustain the self-renewal potential of LCSCs by upregulating the expression of SRY-box transcription factor 2 (SOX2) and octamer-binding transcription factor 4 (OCT4).
Article
Engineering, Biomedical
Xiayi Xu, Qian Feng, Xun Ma, Yingrui Deng, Kunyu Zhang, Hon Son Ooi, Boguang Yang, Zhi-Yong Zhang, Bo Feng, Liming Bian
Summary: In this study, a gelatin-based structurally dynamic hydrogel was developed to emulate the intrinsic properties of the extracellular matrix. The dynamic hydrogel significantly promoted the growth and pluripotency maintenance of embryonic stem cells (ESCs).
Article
Multidisciplinary Sciences
Tee Udomlumleart, Sofia Hu, Salil Garg
Summary: Research suggests that some highly dynamic ESC lineages do not exhibit Markovian properties, with previous states and kin relations influencing future choices. Surprisingly, the distribution of lineages among states remains constant over time, contradicting predictions from a Markov model. Highly dynamic ESC lineages also show skewed cell fate distributions post differentiation.
Article
Biochemistry & Molecular Biology
Lucas Simoes Machado, Camila Martins Borges, Marina Amaro de Lima, Juliano Rodrigues Sangalli, Jacinthe Therrien, Lais Vicari de Figueiredo Pessoa, Paulo Fantinato Neto, Felipe Perecin, Lawrence Charles Smith, Flavio Vieira Meirelles, Fabiana Fernandes Bressan
Summary: Mechanisms of cell reprogramming by pluripotency-related transcription factors or nuclear transfer seem to be mediated by similar pathways. The study suggests that OCT4 and SOX2 may contribute to both processes and help elucidate the mechanisms responsible for pluripotency.
Article
Cell Biology
Chen Gao, Xiaolan Qi, Xin Gao, Jin Li, Yumin Qin, Yunjun Yin, Fei Gao, Tao Feng, Sen Wu, Xuguang Du
Summary: Screening with a CRISPR library under stringent conditions revealed key regulators of pluripotency exit, including previously reported genes and 17 unreported genes. Zfp771 and Olfr769 were found to be potentially involved in pluripotency exit.
Article
Biochemistry & Molecular Biology
L. E. Wadkin, S. Orozco-Fuentes, I Neganova, M. Lako, R. A. Barrio, A. W. Baggaley, N. G. Parker, A. Shukurov
Summary: Enhancing the regulation of human embryonic stem cell pluripotency and differentiation trajectories is vital for their clinical applications. Analyzing the temporal and spatial dynamics of the pluripotency transcription factor OCT4 in hESC colonies reveals insights into its self-regulation and fluctuations within cells. Understanding the similarities in OCT4 expression among neighboring cells can provide valuable information about the behavior of proliferating hESC colonies.
Article
Chemistry, Multidisciplinary
Xupeng Bai, Jie Ni, Julia Beretov, Shanping Wang, Xingli Dong, Peter Graham, Yong Li
Summary: The study shows that THOC2 and THOC5 are upregulated in radioresistant TNBC cells, promoting stem-like properties and radioresistance. Silencing THOC2 or THOC5 expression decreases protein expression of SOX2 and NANOG, depletes stem-like properties, and causes radiosensitization in TNBC cells. Depletion of THOC2 or THOC5 blocks xenograft tumorigenesis and growth of radioresistant TNBC in vivo, suggesting potential therapeutic strategies against relapsed TNBC.
Article
Cell Biology
Robert Blassberg, Harshil Patel, Thomas Watson, Mina Gouti, Vicki Metzis, M. Joaquina Delas, James Briscoe
Summary: This study reveals that the level of transcription factor SOX2 determines the chromatin occupancy of TCF/β-catenin, thus regulating the cell fate and gene expression during embryonic development.
NATURE CELL BIOLOGY
(2022)
Article
Gastroenterology & Hepatology
Liseth Rivero-Sanchez, Aleksandar Gavric, Jesus Herrero, David Remedios, Victoria Alvarez, Eduardo Albeniz, Jordi Gordillo, Ignasi Puig, Jorge Lopez-Vicente, Alain Huerta, Maria Lopez-Ceron, Inmaculada Salces, Beatriz Penas, Sofia Parejo, Enrique Rodriguez, Maite Herraiz, Cristina Carretero, Antonio Z. Gimeno-Garcia, Esteban Saperas, Cristina Alvarez, Coral Arnau-Collell, Oswaldo Ortiz, Ariadna Sanchez, Gerhard Jung, Francesc Balaguer, Maria Pellise
Summary: The study showed that the diagnose-and-leave-in strategy is feasible and safe for treating diminutive rectosigmoid polyps in patients with Lynch syndrome, which can help avoid unnecessary polypectomies.
Article
Gastroenterology & Hepatology
Olga P. Nyssen, Dino Vaira, Bojan Tepes, Limas Kupcinskas, Dmitry Bordin, Angeles Perez-Aisa, Antonio Gasbarrini, Manuel Castro-Fernandez, Luis Bujanda, Ana Garre, Alfredo Lucendo, Liudmila Vologzhanina, Natasa B. Jurecic, Luis Rodrigo-Saez, Jose M. Huguet, Irina Voynovan, Jorge Perez-Lasala, Pilar Mata Romero, Miroslav Vujasinovic, Rustam Abdulkhakov, Jesus Barrio, Luis Fernandez-Salazar, Francis Megraud, Colm O'Morain, Javier P. Gisbert
Summary: Based on the European Registry on Helicobacter pylori management, this study evaluated common mistakes in the eradication of H. pylori infection. Results showed that some European gastroenterologists made common mistakes in the management, such as using ineffective treatment, prescribing short-term therapy, and using low doses of medications. These errors led to suboptimal treatment outcomes. Although clinical practice is slowly adapting to current recommendations, there is still a delay in adopting updated guidelines.
JOURNAL OF CLINICAL GASTROENTEROLOGY
(2022)
Article
Gastroenterology & Hepatology
Mikel Ruiz de Gauna, Francesca Biancaniello, Francisco Gonzalez-Romero, Pedro M. Rodrigues, Ainhoa Lapitz, Beatriz Gomez-Santos, Paula Olaizola, Sabina Di Matteo, Igor Aurrekoetxea, Ibone Labiano, Ane Nieva-Zuluaga, Asier Benito-Vicente, Maria J. Perugorria, Maider Apodaka-Biguri, Nuno A. Paiva, Diego Saenz de Urturi, Xabier Buque, Igotz Delgado, Cesar Martin, Mikel Azkargorta, Felix Elortza, Diego F. Calvisi, Jesper B. Andersen, Domenico Alvaro, Vincenzo Cardinale, Luis Bujanda, Jesus M. Banales, Patricia Aspichueta
Summary: This study found that highly proliferative CCA cells rely on lipid and lipoprotein uptake to fuel fatty acid catabolism, suggesting that inhibition of FAO and/or lipid uptake could represent a therapeutic strategy for this CCA subclass.
Article
Biochemistry & Molecular Biology
Rokas Lukosevicius, Simonas Juzenas, Violeta Salteniene, Ugne Kulokiene, Justina Arstikyte, Georg Hemmrich-Stanisak, Andre Franke, Alexander Link, Paulius Ruzgys, Saulius Satkauskas, Henrikas Pauzas, Tadas Latkauskas, Gediminas Kiudelis, Francesc Balaguer, Juozas Kupcinskas, Jurgita Skieceviciene
Summary: This study explored regulatory changes in early colorectal cancer development and identified differentially expressed miRNAs using small RNA-seq profiling of colon biopsy samples. Functional experiments revealed the oncogenic effect of hsa-miR-1246 and its involvement in regulating the expression of tumor suppressors AXIN2 and CFTR.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Yamile Zabana, Ignacio Marin-Jimenez, Iago Rodriguez-Lago, Isabel Vera, Maria Dolores Martin-Arranz, Ivan Guerra, Javier P. Gisbert, Francisco Mesonero, Olga Benitez, Carlos Taxonera, Angel Ponferrada-Diaz, Marta Piqueras, Alfredo J. Lucendo, Berta Caballol, Miriam Manosa, Pilar Martinez-Montiel, Maia Bosca-Watts, Jordi Gordillo, Luis Bujanda, Noemi Mancenido, Teresa Martinez-Perez, Alicia Lopez, Cristina Rodriguez-Gutierrez, Santiago Garcia-Lopez, Pablo Vega, Montserrat Rivero, Luigi Melcarne, Maria Calvo, Marisa Iborra, Manuel Barreiro de-Acosta, Beatriz Sicilia, Jesus Barrio, Jose Lazaro Perez, David Busquets, Isabel Perez-Martinez, Merce Navarro-Llavat, Vicent Hernandez, Federico Argueelles-Arias, Fernando Ramirez Esteso, Susana Meijide, Laura Ramos, Fernando Gomollon, Fernando Munoz, Gerard Suris, Jone Ortiz de Zarate, Jose Maria Huguet, Jordina Llao, Mariana Fe Garcia-Sepulcre, Monica Sierra, Miguel Dura, Sandra Estrecha, Ana Fuentes Coronel, Esther Hinojosa, Lorenzo Olivan, Eva Iglesias, Ana Gutierrez, Pilar Varela, Nuria Rull, Pau Gilabert, Alejandro Hernandez-Camba, Alicia Brotons, Daniel Ginard, Eva Sese, Daniel Carpio, Montserrat Aceituno, Jose Luis Cabriada, Yago Gonzalez-Lama, Laura Jimenez, Maria Chaparro, Antonio Lopez-San Roman, Cristina Alba, Rocio Plaza-Santos, Raquel Mena, Sonsoles Tamarit-Sebastian, Elena Ricart, Margalida Calafat, Sonsoles Olivares, Pablo Navarro, Federico Bertoletti, Horacio Alonso-Galan, Ramon Pajares, Pablo Olcina, Pamela Manzano, Eugeni Domenech, Maria Esteve
Summary: This study describes the incidence and source of contagion of COVID-19 in patients with inflammatory bowel disease (IBD), as well as the risk factors for a severe course and long-term sequelae. The results show that the prognosis of COVID-19 in IBD patients, even in those on immunosuppression, is similar to that in the general population, indicating that there is no need for more strict protection measures in IBD.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Medicine, General & Internal
Ana Gutierrez, Pedro Zapater, Elena Ricart, Maria Gonzalez-Vivo, Jordi Gordillo, David Olivares, Isabel Vera, Miriam Manosa, Javier P. Gisbert, Mariam Aguas, Eugenia Sanchez-Rodriguez, Maia Bosca-Watts, Viviana Laredo, Blau Camps, Ignacio Marin-Jimenez, Yamile Zabana, Maria Dolores Martin-Arranz, Roser Munoz, Merce Navarro, Eva Sierra, Lucia Madero, Milagros Vela, Jose Lazaro Perez-Calle, Empar Sainz, Xavier Calvet, Lara Arias, Victor Morales, Fernando Bermejo, Luis Fernandez-Salazar, Manuel Van Domselaar, Luisa De Castro, Cristina Rodriguez, Carmen Munoz-Villafranca, Rufo Lorente, Montserrat Rivero, Eva Iglesias, Belen Herreros, David Busquets, Joan Riera, Maria Pilar Martinez-Montiel, Marta Roldon, Oscar Roncero, Esther Hinojosa, Monica Sierra, Jesus Barrio, Ruth De Francisco, Jose Huguet, Olga Merino, Daniel Carpio, Daniel Ginard, Fernando Munoz, Marta Piqueras, Pedro Almela, Federico Argueelles-Arias, Guillermo Alcain, Luis Bujanda, Noemi Mancenido, Alfredo J. Lucendo, Pilar Varela, Iago Rodriguez-Lago, Laura Ramos, Laura Sempere, Eva Sese, Manuel Barreiro-de Acosta, Eugeni Domenech, Ruben Frances
Summary: Compared with native-born patients, first-generation-immigrant IBD patients in Spain show differences in disease onset age, extraintestinal manifestations, and biologics usage.
FRONTIERS IN MEDICINE
(2022)
Article
Oncology
Yasuyuki Okada, Fuduan Peng, Jose Perea, Luis Corchete, Luis Bujanda, Wei Li, Ajay Goel
Summary: A novel methylation signature risk-scoring model was developed to reliably identify patients with synchronous colorectal cancer (SyCRC), which has the potential to improve the diagnosis and management of colorectal cancer (CRC) patients.
BRITISH JOURNAL OF CANCER
(2023)
Article
Gastroenterology & Hepatology
Antonio Giordano, Gherzon Casanova, Miriam Escapa, Gloria Fernandez-Esparrach, Angels Gines, Oriol Sendino, Isis K. Araujo, Andres Cardenas, Henry Cordova, Julia Martinez-Ocon, Graciela Martinez-Palli, Francesc Balaguer, Josep Llach, Elena Ricart, Begona Gonzalez-Suarez
Summary: Motorized Spiral Enteroscopy (MSE) is an effective and safe examination method for patients with prior major abdominal surgery. Although longer procedure times were observed in these patients, the diagnostic yield was similar. Patients with gastrointestinal surgery showed a lower depth of insertion compared to those with other abdominal surgeries.
DIGESTIVE DISEASES AND SCIENCES
(2023)
Article
Genetics & Heredity
Yasmin Soares de Lima, Coral Arnau-Collell, Jenifer Munoz, Cristina Herrera-Pariente, Leticia Moreira, Teresa Ocana, Marcos Diaz-Gay, Sebastia Franch-Exposito, Miriam Cuatrecasas, Sabela Carballal, Anael Lopez-Novo, Lorena Moreno, Guerau Fernandez, Aranzazu Diaz de Bustamante, Sophia Peters, Anna K. Sommer, Isabel Spier, Iris B. A. W. te Paske, Yasmijn J. van Herwaarden, Antoni Castells, Luis Bujanda, Gabriel Capella, Verena Steinke-Lange, Khalid Mahmood, JiHoon Eric Joo, Julie Arnold, Susan Parry, Finlay A. Macrae, Ingrid M. Winship, Christophe Rosty, Joaquin Cubiella, Daniel Rodriguez-Alcalde, Elke Holinski-Feder, Richarda de Voer, Daniel D. Buchanan, Stefan Aretz, Clara Ruiz-Ponte, Laura Valle, Francesc Balaguer, Laia Bonjoch, Sergi Castellvi-Bel
Summary: Rare germline variants in the WNK2 gene were identified through functional evaluation of a candidate gene and replication in additional SPS cohorts. These variants may affect protein function in the MAPK pathway, thereby increasing the risk for SPS.
JOURNAL OF MEDICAL GENETICS
(2023)
Letter
Biochemistry & Molecular Biology
Keisuke Okuno, Raju Kandimalla, Marta Mendiola, Francesc Balaguer, Luis Bujanda, Carlos Fernandez-Martos, Jorge Aparicio, Jaime Feliu, Masanori Tokunaga, Yusuke Kinugasa, Joan Maurel, Ajay Goel
Article
Gastroenterology & Hepatology
Francisco J. Caballero-Camino, Pedro M. Rodrigues, Fredrik Wangsell, Alona Agirre-Lizaso, Paula Olaizola, Laura Izquierdo-Sanchez, Maria J. Perugorria, Luis Bujanda, Bo Angelin, Sara Straniero, Anna Walleback, Ingemar Starke, Per-Goran Gillberg, Ellen Strangberg, Britta Bonn, Jan P. Mattsson, Martin R. Madsen, Henrik H. Hansen, Erik Lindstrom, Peter Akerblad, Jesus M. Banales
Summary: The ASBT inhibitor A3907 improves cholestatic liver diseases by clearing circulating bile acids and protecting liver function. A3907 targets ASBT in the intestine, liver, and kidney, leading to improved bilirubin and liver injury markers. A3907 is well tolerated in healthy individuals, supporting further clinical development.
Article
Gastroenterology & Hepatology
Joel Flores-Arriaga, Maria C. Aso, Arantzazu Izagirre, Ami D. Sperber, Olafur S. Palsson, Shrikant I. Bangdiwala, Angel Lanas, Luis Bujanda, Beatriz Lobo, Carmen Alonso-Cotoner, Alex Sanchez-Pla, Javier Santos
Summary: The study used data from the Rome Foundation Global Epidemiology Study to assess the prevalence and burden of gut-brain disorders in Spain. The survey, completed by 2072 participants, revealed that 43.6% of respondents met diagnostic criteria for at least one disorder, with functional constipation being the most prevalent. The study highlights the need for specialized training and future research in this area.
NEUROGASTROENTEROLOGY AND MOTILITY
(2023)
Meeting Abstract
Gastroenterology & Hepatology
Jose Perea, Luis Antonio Corchete, Kevin J. Monahan, Antonino Spinelli, Caterina Foppa, Noel F. de Miranda, Myr Abdulrahman, Claire Palles, Helen M. Curley, Francesc Balaguer, Maria de los Angeles Daca Alvarez, Marc Marti-Gallostra, Mireia Verdaguer, Alfredo Vivas, Andrew Latchford, Omar Faiz, Nikhil Pawa, Rabiya Aseem, Wieslaw Tarnowski, Marek Szczepkowski, Katarzyna Borycka-Kiciak, Silviu-Tiberiu Makkai-Popa, Juan S. Azagra, Damian Garcia-Olmo, Rogelio Gonzalez-Sarmiento, Andreana Holowatyj
Meeting Abstract
Biochemistry & Molecular Biology
Anael Lopez-Novo, Juan Fernandez-Tajes, Jorge Amigo, Andres Dacal, David Remedios, Joaquin Cubiella, Victoria Alvarez-Sanchez, Maria Jesus Ladra-Gonzalez, Fernando Fernandez-Lopez, Ana Alvarez-Castro, Jose Manuel Cameselle-Teijeiro, Miriam Cuatrecasas, Francesc Balaguer, Sergi Castellvi-Bel, Ceres Fernandez-Rozadilla, Clara Ruiz-Ponte
EUROPEAN JOURNAL OF HUMAN GENETICS
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Cristina Herrera-Pariente, Roser Capo-Garcia, Marcos Diaz-Gay, Sabela Carballal, Jenifer Munoz, Joan Llach, Ariadna Sanchez, Laia Bonjoch, Coral Arnau-Collell, Yasmin Soares de Lima, Mariano Golubicki, Gerhard Jung, Juan Jose Lozano, Antoni Castells, Francesc Balaguer, Luis Bujanda, Sergi Castellvi-Bel, Leticia Moreira
EUROPEAN JOURNAL OF HUMAN GENETICS
(2022)