期刊
WORLD JOURNAL OF GASTROENTEROLOGY
卷 19, 期 30, 页码 4925-4934出版社
BAISHIDENG PUBL GRP CO LTD
DOI: 10.3748/wjg.v19.i30.4925
关键词
Tumor necrosis factor-alpha; Intestine; Mucosa; Apoptosis; c-Jun N-terminal kinase
资金
- Major Projects Incubator Program of the National Key Basic Research Program of China [2012CB526700]
- National Natural Science Foundation of China [30971357]
- Natural Science Foundation of Guangdong Province [S2011020002348]
- Science and Technology Planning Project of Guangdong Province [2009B060300001]
- Major Projects Incubator Program of Sun Yat-Sen University [10ykjc25]
AIM: To investigate whether tumor necrosis factor-alpha (TNF-alpha) mediates ischemia-reperfusion (I/R)-induced intestinal mucosal injury through c-Jun N-terminal kinase (JNK) activation. METHODS: In this study, intestinal I/R was induced by 60-min occlusion of the superior mesenteric artery in rats followed by 60-min reperfusion, and the rats were pretreated with a TNF-alpha inhibitor, pentoxifylline, or the TNF-alpha antibody infliximab. After surgery, part of the intestine was collected for histological analysis. The mucosal layer was harvested for RNA and protein extraction, which were used for further real-time polymerase chain reaction, enzyme-linked immunosorbent assay and Western blotting analyses. The TNF-alpha expression, intestinal mucosal injury, cell apoptosis, activation of apoptotic protein and JNK signaling pathway were analyzed. RESULTS: I/R significantly enhanced expression of mucosal TNF-alpha at both the mRNA and protein levels, induced severe mucosal injury and cell apoptosis, activated caspase-9/caspase-3, and activated the JNK signaling pathway. Pretreatment with pentoxifylline markedly downregulated TNF-alpha at both the mRNA and protein levels, whereas infliximab pretreatment did not affect the expression of TNF-alpha induced by I/R. However, pretreatment with pentoxifylline or infliximab dramatically suppressed I/R-induced mucosal injury and cell apoptosis and significantly inhibited the activation of caspase-9/3 and JNK signaling. CONCLUSION: The results indicate there was a TNF-alpha-mediated JNK activation response to intestinal I/R injury. (c) 2013 Baishideng. All rights reserved.
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