期刊
WORLD JOURNAL OF GASTROENTEROLOGY
卷 17, 期 9, 页码 1219-1226出版社
BAISHIDENG PUBLISHING GROUP INC
DOI: 10.3748/wjg.v17.i9.1219
关键词
Metastasis-associated protein 1; Vascular endothelial growth factor-C; Lymphangiogenesis; Colorectal cancer
资金
- National Natural Science Foundation of China [30772131]
- Ministry of Education of China [21609313]
AIM: To study the correlation between high metastasis-associated protein 1 (MTA1) expression and lymphangiogenesis in colorectal cancer (CRC) and its role in production of vascular endothelial growth factor-C(VEGF-C). METHODS: Impact of high MTA1 and VEGF-C expression levels on disease progression and lymphovascular density (LVD, D2-40-immunolabeled) in 81 cases of human CRC was evaluated by immunohistochemistry. VEGF-C mRNA and protein expressions in human LoVo and HCT116 cell lines were detected by real-time polymerase chain reaction and Western blotting, respectively, with a stable expression vector or siRNA. RESULTS: The elevated MTA1 and VEGF-C expression levels were correlated with lymph node metastasis and Dukes stages (P < 0.05). Additionally, high MTA1 expression level was correlated with a large tumor size (P < 0.05). A significant correlation was found between MTA1 and VEGF-C protein expressions in tumor cells (r = 0.371, P < 0.05). Similar to the VEGF-C expression level, high MTA1 expression level was correlated with high LVD in CRC (P < 0.05). Furthermore, over-expression of MTA1 significantly enhanced the VEGF-C mRNA and protein expression levels, whereas siRNAs - knocked down MTA1 decreased the VEGF-C expression level. CONCLUSION: MTA1, as a regulator of tumor-associated lymphangiogenesis, promotes. lymphangiogenesis in CRC by mediating the VEGF-C expression. (c) 2011 Baishideng. All rights reserved.
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