期刊
WORLD JOURNAL OF GASTROENTEROLOGY
卷 17, 期 5, 页码 625-632出版社
BAISHIDENG PUBLISHING GROUP INC
DOI: 10.3748/wjg.v17.i5.625
关键词
CXCR4; Stromal cell-derived factor-1; E-cadherin; beta-catenin; Phosphatidylinositol 3-kinase/AKT; Colon cancer
资金
- National Natural Science Foundation of China [30571712, 30810403081]
AIM: To study the influence of CXCR4/stromal cell-derived factor-1 (SDF-1) axis on E-cadherin/beta-catenin complex expression in HT29 colon cancer cells and its underlying mechanisms. METHODS: Effect of SDF-1 on E-cadherin/beta-catenin expression was detected by immunocytochemistry. E-cadherin and p-catenin mRNA expression levels were measured by reverse transcriptase-polymerase chain reaction. SDF-1-induced phosphorylation of phosphatidylinositol 3-kinase (PI3K)/AKT and p-catenin was detected by Western blotting. RESULTS: The E-cadherin and beta-catenin mRNA expression levels in HT29 cells were lower 48 h after incubated with SDF-1 at the concentrations of 20 and 40 ng/mL (P < 0.05). SDF-1-induced significant phosphorylation of PI3K/AKT and p-catenin. AMD3100 and LY294002 inhibited the phosphorylation cif PI3K/AKT and beta-catenin. CONCLUSION: SDF-1 down-regulates the E-cadherin/beta-catenin complex expression in HT29 cells by decreasing mRNA synthesis and increasing p-catenin phosphorylation. (C) 2011 Baishideng. All rights reserved.
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