期刊
WORLD JOURNAL OF GASTROENTEROLOGY
卷 16, 期 23, 页码 2851-2866出版社
BAISHIDENG PUBLISHING GROUP INC
DOI: 10.3748/wjg.v16.i23.2851
关键词
Liver; Tissue fixation; Perfusion; Electron microscopy; Biopsy
For an electron microscopic study of the liver, expertise and complicated, time-consuming processing of hepatic tissues and cells is needed. The interpretation of electron microscopy (EM) images requires knowledge of the liver fine structure and experience with the numerous artifacts in fixation, embedding, sectioning, contrast staining and microscopic imaging. Hence, the aim of this paper is to present a detailed summary of different methods for the preparation of hepatic cells and tissue, for the purpose of preserving long-standing expertise and to encourage new investigators and clinicians to include EM studies of liver cells and tissue in their projects. (C) 2010 Baishideng. All rights reserved.
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