期刊
WORLD JOURNAL OF GASTROENTEROLOGY
卷 15, 期 32, 页码 4016-4022出版社
BAISHIDENG PUBLISHING GROUP INC
DOI: 10.3748/wjg.15.4016
关键词
Proton pump; H,K-ATPase; Parietal cell; Gastric gland; Oxyntic mucosa; Cigarette smoke extract; Smoking
资金
- UAE University
- Terry Fox Foundation
AIM: To test whether the expression and activity of H,K-ATPase in parietal cells would be affected by cigarette smoke extract. METHODS: Extracts of cigarette smoke were administered into mice by gastric gavage (5 mg/kg body weight/day) for 3 d or in drinking water for 7 or 14 d. For the latter, each day a mouse consumed 5 mL water containing extracts of two cigarettes, on average. Control littermate mice received only vehicle. To compare the amount of H,K-ATPase in control and smoke-treated mice, the stomach was processed for Western blotting and immunohistochemical analysis using monoclonal antibodies specific for alpha- or beta-subunits of H,K-ATPase. The p-nitrophenylphospatase activity assay was used as a measurement for K-dependent H,K-ATPase activity. RESULTS: Probed transblots showed an increase in the amount of H,K-ATPase in smoke-treated mice which was confirmed by immunohistochemistry and was found to be due to increased amounts of protein per parietal cell rather than an increased parietal cell number. The increase in the amount of H,K-ATPase was associated with an enhancement of its enzymatic activity. K-dependent activity in control and smoke-treated mice was significantly different (respectively, 0.12 mu mol/mg vs 0.27 mu mol/mg per minute, P < 0.05). CONCLUSION: Administration of cigarette smoke extract is associated with an increase in the amount and activity of H,K-ATPase and hence, smokers are susceptible to development of peptic ulcer. (C) 2009 The WJG Press and Baishideng. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据