Article
Plant Sciences
Maolong Hu, Hongkun Zhang, Lingna Kong, Juanjuan Ma, Ting Wang, Xinyu Lu, Yue Guo, Jiefu Zhang, Rongzhan Guan, Pu Chu
Summary: The application of herbicides is an effective strategy for weed control, and the development of herbicide-resistant crops can enhance weed management. However, the use of the herbicide tribenuron-methyl (TBM) is limited in rapeseed fields due to rapeseed's sensitivity to TBM. A study was conducted on the TBM-resistant rapeseed mutant M342 and its wild-type plants, revealing that M342 exhibited improved tolerance to TBM and had higher levels of proteins related to non-target-site resistance (NTSR) to herbicides compared to the wild-type plants. Differentially accumulated proteins (DAPs) between the two genotypes were enriched in glutathione metabolism and oxidoreduction coenzyme metabolic process, providing protection against oxidative stress caused by TBM. These findings offer insights into the mechanism of NTSR in plants and contribute to the development of herbicide-resistant crops.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Caiyue Liu, Tianbao Zhang, Xinsen Yang, Liu Wang, Yan Long, Agula Hasi, Xinwu Pei
Summary: In this study, a mutant with resistance to ALS-inhibiting herbicides was obtained from the elite cultivar Longya10 through EMS mutagenesis. The mutant showed significantly increased resistance to TBM and slight resistance to imazethapyr compared to Longya10. Biochemical analysis revealed a Pro197Ser mutation in the LuALS1 gene, which conferred high resistance to sulfonylurea herbicides in the mutant. Two CAPS markers were developed based on this mutation site for marker assistant selection in breeding. The mutant did not cause yield losses in natural field conditions, making it a valuable germplasm resource for breeding herbicide-resistant flax varieties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agronomy
Magdalena Walkowiak, Marcin Matuszczak, Stanislaw Spasibionek, Alina Liersch, Katarzyna Mikolajczyk
Summary: In our research, we used new markers specific to the LuFAD3A gene to differentiate between different varieties of flax. We also proved that the LuFAD3A gene mutation previously detected in low-linolenic flax was absent in the low-linolenic flax studied by us.
Article
Agronomy
Lei Chen, Gang Gu, Chengxu Wang, Zhufeng Chen, Wei Yan, Man Jin, Gang Xie, Junli Zhou, Xing Wang Deng, Xiaoyan Tang
Summary: Herbicide resistance is valuable for integrated weed management in agriculture, particularly in rice fields where weedy rice poses a challenge. This study obtained three mutant variants of the OsALS gene in rice through ethyl methyl sulfonate mutagenesis, with W-548 M mutant showing high levels of resistance to a broad spectrum of herbicides. The stable inheritance of herbicide resistance in rice lines makes W-548 M mutation a valuable resource for breeding herbicide resistant rice and weed management.
Article
Plant Sciences
Sarah Shah, Thierry Lonhienne, Cody-Ellen Murray, Yibi Chen, Katherine E. Dougan, Yu Shang Low, Craig M. Williams, Gerhard Schenk, Gimme H. Walter, Luke W. Guddat, Cheong Xin Chan
Summary: This study identified 12 common herbicide-target genes and their proteins from seven species of Weeds of National Significance in Australia using high-throughput genome sequencing. The study found high sequence conservation at the herbicide-target sites among the diverse weed species. Mutations observed in the proteins of fireweed and parthenium suggest resistance of these weeds to certain herbicides. These findings provide important insights for herbicide discovery and development.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biology
Rui Zhang, Sha Chen, Xiangbing Meng, Zhuangzhuang Chai, Delin Wang, Yuge Yuan, Kunling Chen, Linjian Jiang, Jiayang Li, Caixia Gao
Summary: Utilizing genome editing, various missense mutations were created in rice to confer tolerance to different herbicides, with a novel triple mutant showing high tolerance to all tested herbicides. Field trials suggested that these mutated varieties have potential practical applications in weed control.
SCIENCE CHINA-LIFE SCIENCES
(2021)
Article
Agronomy
Yi Cao, Xinxin Zhou, Zhaofeng Huang
Summary: The study revealed the molecular basis for resistance of Amaranthus retroflexus to ALS-inhibiting herbicides, with Trp-574-Leu and Gly-654-Tyr substitutions in ALS contributing to broad-spectrum resistance. The Gly-654-Tyr substitution, previously unreported, conferred high resistance to multiple classes of ALS-inhibiting herbicides in the R-Tyr654 population. Despite this resistance, effective control of the population was achieved with herbicides targeting other sites of action.
PEST MANAGEMENT SCIENCE
(2022)
Article
Plant Sciences
Ryan Widianto, Denny Kurniadie, Dedi Widayat, Uum Umiyati, Ceppy Nasahi, Santika Sari, Abdul Shukor Juraimi, Hisashi Kato-Noguchi
Summary: Monochoria vaginalis is an aquatic herbaceous plant that is often found as a noxious weed in paddy fields. This research evaluated the resistance levels of M. vaginalis to different groups of herbicides and investigated the mutations in the ALS gene of the resistant biotype. The study found that the resistant biotype had varying levels of resistance to different herbicides and had specific amino acid substitutions in the ALS protein. Further research is needed to understand the molecular basis of resistance mechanisms and to develop control strategies.
Article
Horticulture
Yashu Yang, Yan Zhang, Chenyang Huang, Qiang Chen, Wei Gao
Summary: This study developed CAPS/dCAPS markers for early visual identification of cap color in oyster mushrooms, facilitating the selection and breeding of dark-colored varieties.
Article
Biotechnology & Applied Microbiology
Wegi A. Wuddineh, Xia Xu, Gan-Yuan Zhong
Summary: This study identified VvALS1 as the best candidate gene for providing resistance to ALS-inhibiting herbicides in grapevine. The researchers found that chlorsulfuron and imazapyr herbicides showed a linear response curve in grapevine callus and in vitro shoots. By introducing amino acid substitutions in VvALS gene, the transgenic plants exhibited high resistance to chlorsulfuron or imazapyr herbicides. This research highlights the potential use of VvALS mutations as a selectable marker and enables gene-editing in grapevine improvement.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Plant Sciences
Shivani, Satvir Kaur Grewal, Ranjit Kaur Gill, Harpreet Kaur Virk, Rachana D. Bhardwaj
Summary: Yield reduction in lentil crop due to weed infestation is a major obstacle to its growth. This study investigated the impact of the selective herbicide imazethapyr (IM) on lentil genotypes. IM treatment led to a decrease in chlorophyll and carotenoid content, particularly in susceptible genotypes. Susceptible genotypes also showed reduced plant height, lower number of pods, and decreased seed yield under IM treatment. In contrast, tolerant genotypes had higher acetolactate synthase (ALS) activity, antioxidant capacity, and glycine betaine content, as well as less decrease in relative leaf water content, resulting in higher seed yield. These findings suggest that the identified IM tolerance mechanism can be used to enhance herbicide resistance in lentil.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2022)
Article
Agronomy
Tiancheng Lou, Ke Wang, Junmin Chen, Jingjing Cao, Tao Gu, Liben Jiang, Yuanlai Lou, Rongxiang Cao, Hongchun Wang
Summary: Tribenuron-methyl-resistant cleavers have been reported in the major winter wheat farming region in China. The study evaluated the frequency and distribution of multiple herbicide resistance in cleavers populations across Jiangsu Province from 2017 to 2020. The results highlight the importance of diversity in herbicide sites of action for effective weed management.
Article
Plant Sciences
Mikel Vicente Eceiza, Maria Barco-Antonanzas, Miriam Gil-Monreal, Michiel Huybrechts, Ana Zabalza, Ann Cuypers, Mercedes Royuela
Summary: The aim of this study was to investigate the role of oxidative stress in the mode of action of ALS inhibiting herbicides. Two populations of Amaranthus palmeri S. Watson from Spain (sensitive and resistant to nicosulfuron, due to mutated ALS) were subjected to nicosulfuron treatment at different rates. The results showed that oxidative stress is linked to ALS inhibition, but a supralethal nicosulfuron rate was needed to induce a significant oxidative stress response in the sensitive population, indicating that ALS inhibitors' lethality is not entirely dependent on oxidative stress.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agronomy
Barbara Wrzesinska, Tadeusz Praczyk
Summary: Centaurea cyanus, a weed species commonly found in fields with cereals, sugar beet, and maize, shows resistance mainly against acetolactate synthase (ALS) inhibitors. Genetic variability in ALS sequence has been observed among resistant plants, with four amino acid sequence changes identified in some resistant plants compared to susceptible ones. Further research is needed to confirm the significance of these mutations in herbicide resistance development.
Article
Agronomy
Alysha T. Torbiak, Robert E. Blackshaw, Randall N. Brandt, Bill Hamman, Charles M. Geddes
Summary: Kochia is an invasive weed in North America that affects crop harvest and yield losses. The use of herbicides in western Canada is limited due to the rapid evolution of glyphosate and ALS inhibitor-resistant kochia. Field experiments were conducted to determine effective herbicide options for controlling kochia in field pea. The results showed that the combination of carfentrazone + sulfentrazone with imazamox/bentazon can effectively control resistant kochia in field pea, while also mitigating herbicide resistance.
CANADIAN JOURNAL OF PLANT SCIENCE
(2022)
Article
Agronomy
Nathalie Colbach, Bruno Chauvel, Henri Darmency, Christophe Delye, Valerie Le Corre
PEST MANAGEMENT SCIENCE
(2016)
Article
Environmental Sciences
Nathalie Colbach, Alice Fernier, Valerie Le Corre, Antoine Messean, Henri Darmency
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2017)
Article
Environmental Sciences
Nathalie Colbach, Henri Darmency, Alice Fernier, Sylvie Granger, Valerie Le Corre, Antoine Messean
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2017)
Article
Genetics & Heredity
Remi Perronne, Stephanie Gibot-Leclerc, Fabrice Dessaint, Carole Reibel, Valerie Le Corre
Review
Agronomy
Henri Darmency, Nathalie Colbach, Valerie Le Corre
PEST MANAGEMENT SCIENCE
(2017)
Article
Multidisciplinary Sciences
Lucie Meyer, Romain Causse, Fanny Pernin, Romain Scalone, Geraldine Bailly, Bruno Chauvel, Christophe Delye, Valerie Le Corre
Article
Ecology
Lea Frachon, Cyril Libourel, Romain Villoutreix, Sebastien Carrere, Cedric Glorieux, Carine Huard-Chauveau, Miguel Navascues, Laurene Gay, Renaud Vitalis, Etienne Baron, Laurent Amsellem, Olivier Bouchez, Marie Vidal, Valerie Le Corre, Dominique Roby, Joy Bergelson, Fabrice Roux
NATURE ECOLOGY & EVOLUTION
(2017)
Correction
Ecology
Lea Frachon, Cyril Libourel, Romain Villoutreix, Sebastien Carrere, Cedric Glorieux, Carine Huard-Chauveau, Miguel Navascues, Laurene Gay, Renaud Vitalis, Etienne Baron, Laurent Amsellem, Olivier Bouchez, Marie Vidal, Valerie Le Corre, Dominique Roby, Joy Bergelson, Fabrice Roux
NATURE ECOLOGY & EVOLUTION
(2018)
Article
Biochemistry & Molecular Biology
Jean-Paul Soularue, Armel Thoni, Leo Arnoux, Valerie Le Corre, Antoine Kremer
MOLECULAR ECOLOGY RESOURCES
(2019)
Article
Agronomy
Christophe Delye, Severine Michel, Fanny Pernin, Veronique Gautier, Marie Gislard, Charles Poncet, Valerie Le Corre
PEST MANAGEMENT SCIENCE
(2020)
Article
Agronomy
O. Pointurier, S. Gibot-Leclerc, V. Le Corre, C. Reibel, F. Strbik, N. Colbach
Article
Multidisciplinary Sciences
Valerie Le Corre, Mathieu Siol, Yves Vigouroux, Maud Tenaillon, Christophe Delye
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Agronomy
Guillaume Fried, Valerie Le Corre, Tiana Rakotoson, Julie Buchmann, Thomas Germain, Remi Gounon, Helene Royer, Luc Biju-Duval, Emeline Felten, Eric Vieren, Bruno Chauvel
Summary: The use of herbicide-tolerant varieties (HTVs) in sunflower fields leads to lower weed diversity, but has no significant impact on field margin community diversity. The lower weed diversity in HTV fields is mainly attributed to increased herbicide use and shorter crop rotations, while landscape factors play a more important role in field margin diversity. HTV fields have higher abundance of A. artemisiifolia compared to organic and conventional fields.
Article
Multidisciplinary Sciences
Ingvild Loubet, Laetitia Caddoux, Severine Fontaine, Severine Michel, Fanny Pernin, Benoit Barres, Valerie Le Corre, Christophe Delye
Summary: The study found that ragweed resistance to ALS inhibitors is mainly through non-target site resistance (NTSR), with different NTSR mechanisms endowing different resistance patterns. This research provides new data on ragweed resistance to herbicides and emphasizes the importance of local resistance management and mitigating gene flow from populations where resistance has arisen.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Lucie Meyer, Fanny Pernin, Severine Michel, Geraldine Bailly, Bruno Chauvel, Valerie Le Corre, Christophe Delye
Summary: Assessing the capacity of weeds to develop herbicide resistance before it occurs in the field is crucial for effective chemical weed control. This study used herbicide selection and controlled crosses to accelerate the evolution of resistance to two commonly used herbicides in Ambrosia artemisiifolia. The results showed that non-target-site-based resistance (NTSR) to herbicides was significantly increased in just two generations of selection. NTSR to the tested herbicides emerged and was reinforced in subsequent generations. Further bioassays confirmed the emergence of resistance to imazamox and/or tribenuron in multiple A. artemisiifolia populations. NTSR was found to be the predominant type of resistance, with only one population showing a mutant ALS allele in addition to NTSR. This study highlights the importance of pre-emptive assessment of resistance evolution in weed populations.