Article
Plant Sciences
M. Cecilia Pacheco-Insausti, Ivana Tamara Ponce, Miguel A. Quinones, Hilda E. Pedranzani, Jose J. Pueyo, Jozef Kovacik, Ildiko Matusikova
Summary: Inoculation with a salt- and cadmium-tolerant strain of Sinorhizobium meliloti improved the growth of alfalfa plants and reduced cadmium accumulation under salinity and cadmium stress. This was accompanied by increased antioxidant enzymatic activities and the expression of genes related to tolerance mechanisms.
Article
Biochemistry & Molecular Biology
Marie Pacoud, Karine Mandon, Julie Cazareth, Olivier Pierre, Pierre Frendo, Genevieve Alloing
Summary: Reactive oxygen species, such as hydrogen peroxide, play a crucial role in controlling the establishment and functioning of Rhizobium-legume symbiosis. This study used biosensors to measure the intracellular redox state of Sinorhizobium meliloti, a bacterium involved in nitrogen-fixing symbiosis. The results revealed an oxidative shift in the redox status of S. meliloti during bacteroid differentiation. These findings provide new opportunities for studying redox dynamics during N2-fixing symbiosis.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Plant Sciences
Marie-Laure Martin, Marjorie Pervent, Ilana Lambert, Stefano Colella, Mathilde Tancelin, Dany Severac, Gilles Clement, Pascal Tillard, Florian Frugier, Marc Lepetit
Summary: In mature symbiotic root nodules, rhizobia fix atmospheric dinitrogen to provide ammonium for plant nitrogen demand. The symbiosis is adjusted to the plant's nitrogen demand through systemic N signaling, controlling nodule development. Nodule expansion is stimulated under N deficit, while nodule senescence is activated under N satiety. Drought has a negative impact on nodules. In this study, split-root systems were used to characterize the systemic responses of symbiotic plants to localized osmotic stress. The application of PEG inhibited the symbiotic dinitrogen fixation activity of locally treated nodules, leading to nitrogen limitation. The localized PEG treatment triggered systemic signaling, stimulating nodule development and enhancing sucrose allocation. Transcriptomic reprogramming associated with PEG and N deficit signaling shared many target transcripts. These findings suggest that systemic N signaling plays a role in the adaptation of symbiotic plants to local variations in their edaphic environment.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Environmental Sciences
Marie Luyckx, Jean-Francois Hausman, Mathilde Blanquet, Gea Guerriero, Stanley Lutts
Summary: Textile hemp showed potential for phytoremediation purposes due to its ability to efficiently cope with oxidative stress caused by Cd exposure with the help of silicon, which also improved water use efficiency and reduced Cd accumulation in plant organs.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Plant Sciences
Ru-Jie Li, Chun-Xiao Zhang, Sheng-Yao Fan, Yi-Han Wang, Jiangqi Wen, Kirankumar S. Mysore, Zhi-Ping Xie, Christian Staehelin
Summary: This study reveals the presence of a second Nod factor cleaving hydrolase (MtCHIT5b) in Medicago truncatula, which degrades Sinorhizobium meliloti Nod factors in the rhizosphere in conjunction with MtNFH1. The expression of MtCHIT5b is induced by purified Nod factors treatment or rhizobia inoculation, and it is localized in the infection pocket of root hairs. The study also demonstrates that overexpression of MtCHIT5b leads to reduced nodule formation, and simultaneous silencing of MtCHIT5b and MtNFH1 results in decreased nodulation.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Pengbo Liang, Clara Schmitz, Beatrice Lace, Franck Anicet Ditengou, Chao Su, Eija Schulze, Julian Knerr, Robert Grosse, Jean Keller, Cyril Libourel, Pierre-Marc Delaux, Thomas Ott
Summary: Legumes have the ability to associate with rhizobia for nitrogen-fixing root nodule symbiosis. In Medicago truncatula, root hair colonization by Sinorhizobium meliloti begins from young root hairs, which physically entwine around the symbiont to form rhizobial traps. This unique process involves alterations in membrane organization, cytosolic calcium gradient, actin rearrangements, and symbiotic responses in legumes.
Article
Environmental Sciences
Lanya Zhang, Ningning Li, Yawen Wang, Wenjia Zheng, Dandan Shan, Liangliang Yu, Li Luo
Summary: This study demonstrates the importance of ohrR genes in the symbiosis between Sinorhizobium meliloti and alfalfa. These genes affect the infection, nodulation, and nitrogen fixation processes of rhizobia with alfalfa, as well as plant growth. OhrR1 and OhrR3 influence rhizobial growth, while OhrR2 and OhrR3 affect cellular resistance to organic peroxides.
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Prithwi Ghosh, Katie N. Adolphsen, Svetlana N. Yurgel, Michael L. Kahn
Summary: It was found that by introducing specific genes from S. medicae into the S. meliloti strain, the symbiotic effect between rhizobia and plants could be enhanced, promoting plant growth and increasing biomass. These genes related to symbiotic effectiveness may play important roles among different rhizobial strains and could potentially provide new contributions to symbiotic relationships.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Agronomy
Emmanuelle D'Amours, Annick Bertrand, Jean Cloutier, Annie Claessens, Solen Rocher, Philippe Seguin
Summary: This study reveals a relationship between nodule and shoot regrowth after freezing stress, with a higher proportion of undamaged nodules related to increased shoot biomass. The results demonstrate that choosing stress-adapted alfalfa populations and S. meliloti strains can enhance alfalfa persistence.
Article
Microbiology
Agnese Bellabarba, Giovanni Bacci, Francesca Decorosi, Erki Aun, Elisa Azzarello, Maido Remm, Luciana Giovannetti, Carlo Viti, Alessio Mengoni, Francesco Pini
Summary: The association between leguminous plants and symbiotic nitrogen-fixing rhizobia showcases mutualism between eukaryotic hosts and prokaryotic microbes. While this symbiosis is somewhat species-specific, different rhizobial strains can co-colonize the same nodule. Through a bacterial genome-wide association (GWAS) analysis, the study identified genomic determinants related to competitive capabilities in Sinorhizobium meliloti strains.
Article
Plant Sciences
Marie-Francoise Jardinaud, Sebastien Carrere, Benjamin Gourion, Pascal Gamas
Summary: Symbiotic nitrogen fixation plays a key role in reducing the negative impact of nitrogen fertilizers in agroecosystems. The efficiency of this process is influenced by the combination of bacterial and plant genotypes, but the mechanisms behind differences in the efficiency of rhizobium strains are not well known.
PLANT AND CELL PHYSIOLOGY
(2023)
Review
Environmental Sciences
Dilnur Tussipkan, Shuga A. Manabayeva
Summary: Soil contamination is a serious environmental problem that poses risks to human health and the ecosystem. Persistent organic pollutants (POPs) and high concentrations of non-essential heavy metals are global concerns. Phytoremediation, using plants and solar energy, is a green technology for cleaning up pollutants. Alfalfa, with its adaptability and cultivation history, is an ideal plant for remediating contaminated soils. This review provides genomic information on alfalfa, focuses on transgenic plants for enhanced phytoremediation, and discusses future perspectives for improving efficiency.
FRONTIERS IN ENVIRONMENTAL SCIENCE
(2022)
Article
Microbiology
Natalia Soledad Paulucci, Adriana Belen Cesari, Maria Alicia Biasutti, Marta Susana Dardanelli, Maria Angelica Perillo
Summary: The study reveals that the fluidity of Sinorhizobium meliloti membranes changes rapidly under cyclic temperature fluctuations, with different behaviors observed in the outer and inner membranes. After incubation at 40 degrees Celsius, cells were unable to recover the preheating membrane levels at 10 degrees Celsius, leading to delayed formation of structures in Medicago sativa infected with S. meliloti.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Francisco Javier Albicoro, Carolina Vacca, Juan Hilario Cafiero, Walter Omar Draghi, Maria Carla Martini, Mark Goulian, Antonio Lagares, Maria Florencia Del Papa
Summary: To adapt to different environmental conditions, Sinorhizobium meliloti relies on finely tuned regulatory networks, most of which are unexplored to date. Deletion of the two-component system ActJK in S. meliloti negatively affects acid tolerance, bacteroid development, nodule occupancy, and the expression of genes involved in exopolysaccharide synthesis and efflux pumps. The study provides insights into the adaptation of rhizobia to acid stress and highlights the importance of ActJK in regulating key components associated with acid tolerance.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Plant Sciences
Lucia Albornos, Virginia Casado-del-Castillo, Ignacio Martin, Jose M. Diaz-Minguez, Emilia Labrador, Berta Dopico
Summary: The ST1 and ST6 proteins may play a role in primary and lateral root development, as well as symbiotic nodule development. However, only ST6 is involved in the interaction with hemibiotrophic fungi. Their expression is modulated in different ways during plant development and in response to biotic agents, indicating distinct roles in these processes.
Article
Plant Sciences
Noushin Sadeghzadeh, Roghieh Hajiboland, Narges Moradtalab, Charlotte Poschenrieder
Summary: Intercropping enhances nutrient acquisition and chlorophyll concentration, as well as promotes positive interactions between oilseed rape and barley through root exudates. This effect is independent of iron acquisition and makes intercropping an attractive practice, even under non-iron stress conditions.
Article
Plant Sciences
Carol Diaz-Gutierrez, Angela-Tatiana Trillos, Victor Villa, Zulma Silva, Liliana Acevedo, Catalina Arroyave, Charlotte Poschenrieder, Carlos Pelaez
Summary: The study demonstrated the significant impact of altitude and organo-mineral fertilizer on crop production and nutrient uptake in stevia crops, with the organo-mineral fertilizer reducing the effects of altitude differences and improving nutrient uptake and biomass production. This emphasizes the importance of adjusting fertilizer dosing based on crop altitude and highlights the benefits of organo-mineral fertilizer in enhancing soil fertility and reducing contamination risks.
JOURNAL OF PLANT NUTRITION
(2021)
Article
Biochemistry & Molecular Biology
Laura Perez-Martin, Silvia Busoms, Roser Tolra, Charlotte Poschenrieder
Summary: High bicarbonate concentrations in calcareous soils with high pH can impact crop performance, with iron deficiency being a key concern. In this study, comparative transcriptomic analysis of Arabidopsis thaliana demes revealed that carbonate-tolerant plants exhibit better growth and nutrient homeostasis under alkaline conditions, with different gene expression patterns in response to bicarbonate stress compared to sensitive plants. This suggests that carbonate-tolerant plants have a more efficient response to bicarbonate stress, with a focus on stress perception, signal transduction, sulfur acquisition, and cell cycle regulation before iron homeostasis mechanisms are induced.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Silvia Busoms, Joana Teres, Levi Yant, Charlotte Poschenrieder, David E. Salt
Summary: The study revealed that plants in coastal areas adapt locally by tolerating salinity stress, involving a complex interplay of elements such as sodium, copper, and ABA signaling pathways. The results suggest that a specific allele associated with lower molybdenum accumulation and higher copper abundance in plants enhances salinity tolerance in copper-deficient coastal soils through multiple mechanisms.
Article
Plant Sciences
Mabrouka Bouabdallah, Hela Mahmoudi, Tahar Ghnaya, Hedia Hannachi, Ali Taheri, Zeineb Ouerghi, Chiraz Chaffei-Haouari
Summary: The exogenous application of spermidine (Spd) has been found to significantly improve salt stress resistance in durum wheat. Spd treatment increased plant fresh and dry weight, as well as soluble sugars and chlorophyll content, particularly under high salt concentrations. However, Spd treatment led to decreased proline content and glutamate dehydrogenase (GDH) enzyme activity.
PAKISTAN JOURNAL OF BOTANY
(2022)
Article
Environmental Sciences
Ghazwa Boudali, Tahar Ghnaya, Saoussen Ben-Abdallah, Abdallah Chalh, Abdelaziz Sebei, Zeineb Ouirghi, Chiraz Chaffei-Haouari
Summary: In this study, the researchers investigated the effect of homeopathic drug Zincum Metallicum (ZM) on zinc toxicity in Lepidium sativum L, a plant species. They found that ZM was able to alleviate the oxidative damage caused by zinc by reducing zinc accumulation and translocation in the plant.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Daniela Zapata, Catalina Arroyave, Lorena Cardona, Adriana Aristizabal, Charlotte Poschenrieder, Merce Llugany
Summary: Cultivating Spirulina in dairy wastewaters showed higher levels of phytohormones compared to synthetic medium, with low light intensity promoting longer and thicker filament growth, especially in cheese whey. This method allows for cost-effective production of biomass and phytohormones while treating dairy wastewaters.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Agronomy
Laura Perez-Martin, Silvia Busoms, Maria Jose Almira, Nicole Azagury, Joana Teres, Roser Tolra, Charlotte Poschenrieder, Juan Barcelo
Summary: Alkaline salinity constrains crop yield. Arabidopsis thaliana shows local adaptation to saline-siliceous and non-saline carbonate soils, but no natural population is found on saline-alkaline soils. This study suggests that tolerance to saline-siliceous soils may not confer tolerance to alkaline salinity.
Article
Plant Sciences
Maria Jose Almira Casellas, Laura Perez-Martin, Silvia Busoms, Rene Boesten, Merce Llugany, Mark G. M. Aarts, Charlotte Poschenrieder
Summary: By evaluating the growth condition variation of 270 natural accessions of Arabidopsis thaliana on a natural saline-carbonated soil, significant natural variation in leaf sodium and iron concentrations was discovered. The diversity in leaf sodium concentration was associated with allelic variation in the NINJA and YUC8 genes, while leaf iron concentration diversity was associated with allelic variation in the ALA3 gene. The allelic variation in these genes leads to changes in mRNA expression and correlates with plant differential growth performance under alkaline salinity treatment.
Article
Plant Sciences
Roghieh Hajiboland, Aiuob Moradi, Ehsan Kahneh, Charlotte Poschenrieder, Fatemeh Nazari, Jelena Pavlovic, Roser Tolra, Seyed-Yahya Salehi-Lisar, Miroslav Nikolic
Summary: The increased availability of toxic Al3+ is the main limitation for plant growth on acid soils. However, some plants adapted to acid soils can tolerate and accumulate high levels of Al in their aerial parts. Previous studies have focused mostly on acid soils in the northern and southern hemispheres, while acid soils outside of these regions have been neglected. This study investigated the acid soils of tea plantations in Northern Iran and found that several species can accumulate high amounts of Al and Fe, making them suitable for soil rehabilitation.
Article
Plant Sciences
Silvia Busoms, Laura Perez-Martin, Joana Teres, Xin-Yuan Huang, Levi Yant, Roser Tolra, David E. Salt, Charlotte Poschenrieder
Summary: Carbonate-rich soils limit plant performance and crop production. Local adaptation to carbonate soils was detected in two wild Arabidopsis thaliana accessions, A1 (carbonate tolerant) and T6 (carbonate sensitive). Transcriptomic analysis revealed differentially expressed genes related to metabolism and biosynthesis in A1 versus T6 under bicarbonate stress. Comparative genomics and transcriptomics identified 18 genes associated with bicarbonate stress response and soil carbonate tolerance.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Environmental Sciences
Imen Ayachi, Rim Ghabriche, Ameni ben Zineb, Mohsen Hanana, Chedly Abdelly, Tahar Ghnaya
Summary: The effect of sodium chloride (NaCl) on cadmium (Cd) tolerance, uptake, translocation, and compartmentation in barley was studied. NaCl did not significantly affect plant development and biomass production, but Cd alone reduced plant development rate and biomass production. NaCl addition accentuated the Cd effect on plant growth. NaCl limited Cd accumulation in the roots and shoots by reducing Cd-absorption efficiency and translocation. However, NaCl increased Cd toxicity and limited plant yield.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Environmental Sciences
Amira K. Hajri, Noureddine Hamdi, Amnah A. Alharbi, Sahr A. Alsherari, Doha A. Albalawi, Eman Kelabi, Tahar Ghnaya
Summary: Halophytes, especially Sesuvium portulacastrum, have been shown to efficiently accumulate and extract Cd2+ and Zn2+ from saline contaminated water.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Horticulture
Claudia Paola Mora-Cordova, Roser Tolra, Rosa Padilla, Charlotte Poschenrieder, Marie-Helene Simard, Luis Asin, Pere Vilardell, Joan Bonany, Elisabet Claveria, Ramon Dolcet-Sanjuan
Summary: Physiological responses of different interspecific pear hybrids and an open pollinated Pcw to lime induced chlorosis were studied in vitro. The ability to acidify the rhizosphere, rather than iron reduction capacity, was found to be the distinguishing factor in selecting pear hybrid clones adapted to lime-induced chlorosis.
Article
Microbiology
Soledad Martos, Silvia Busoms, Laura Perez-Martin, Merce Llugany, Catalina Cabot, Charlotte Poschenrieder
Summary: The study found that N. brachypetala plants have higher microbiome richness and relative abundance compared to other non-hyperaccumulator Brassicaceae species. The soil microbiota in the rhizosphere may be associated with the plant's ability to absorb metals and potentially contribute to improving soil quality and fertility.
FRONTIERS IN MICROBIOLOGY
(2021)