Article
Environmental Sciences
Xia Jia, Yunfeng Gao, Xiaodi Li, Yonghua Zhao, Lu Wang, Chunyan Zhang
Summary: This study investigated the impacts of elevated CO2 and cadmium levels on soil nitrification in the rhizosphere of Robinia pseudoacacia L. seedlings. The results showed that elevated CO2 combined with Cd pollution generally stimulated nitrification enzyme activities and influenced bacterial community structures.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Soil Science
Shishi He, Yuanwei Li, Hongbin Mu, Zhirong Zhao, Jiawen Wang, Shufeng Liu, Zhenli Sun, Maosheng Zheng
Summary: This study highlights the important role of local ammonium concentration in affecting the ecological niche differentiation of comammox and canonical ammonia-oxidizing prokaryotes. The results show growth differences between CAOB and AOA, with CAOB favoring oligotrophic environments and being more tolerant to a broader range of ammonium concentrations.
APPLIED SOIL ECOLOGY
(2021)
Article
Engineering, Environmental
Aina Soler-Jofra, Lisbeth Schmidtchen, Lluc Olmo, Mark C. M. van Loosdrecht, Julio Perez
Summary: This study investigates the short and long term impact of hydroxylamine on partial nitritation granular sludge. The results indicate that dissolved oxygen is the main factor determining the impact of hydroxylamine. Short term hydroxylamine feeding with low dissolved oxygen leads to higher hydroxylamine accumulation and N2O production, while long term hydroxylamine feeding reduces ammonium consumption rate.
Article
Biodiversity Conservation
Weifang Hu, Mianrun Chen, Xue Lan, Guoliang Li, Bin Wang, Dong Yao Sun, Xianbiao Lin
Summary: This study found significant shifts in potential ammonia oxidation rates (PARs) and gene abundances of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) along different salinity gradients. The PARs were higher under high salinity conditions, and the AOB gene abundance was greater than AOA under moderate and high salinity. This has important implications for nitrogen cycling in estuarine sediments.
ECOLOGICAL INDICATORS
(2022)
Article
Environmental Sciences
Shishi He, Zhirong Zhao, Zhichao Tian, Chi Xu, Yuan Liu, Da He, Yinghui Zhang, Maosheng Zheng
Summary: This study analyzed activated sludge samples from municipal and industrial wastewater treatment plants and found that comammox bacteria were more abundant than ammonia-oxidizing bacteria and ammonia-oxidizing archaea in municipal plants, while this trend did not hold true for industrial plants. Furthermore, a higher diversity of comammox bacteria was found in municipal samples. This study provides important insights into the abundance and diversity of comammox bacteria in municipal and refinery wastewater treatment systems.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Article
Soil Science
Tomohiro Yokobe, Naoko Tokuchi, Fujio Hyodo, Ryunosuke Tateno, Tsutom Hiura
Summary: The effects of nitrogen (N) fertilization on soil microorganisms in a temperate forest dominated by Quercus crispula were investigated. The results showed that N loading increased total carbon (C) concentration and bacterial biomass in the organic layers, while fungal biomass decreased in the mineral soils. These changes may be attributed to the accumulation of recalcitrant C and decreased investment in symbionts by underground plants. These findings contribute to a better understanding of belowground C dynamics.
APPLIED SOIL ECOLOGY
(2022)
Article
Agronomy
Xingjia He, Sen Li, Fengzhi Wu
Summary: Intercropping has a significant impact on soil properties and microbial communities, with different intercropping systems affecting ammonia-oxidizing microorganisms differently in different seasons. Soil physicochemical state, PNR, AOA and AOB communities were significantly altered by intercropping, with seasonal variation playing a greater role in shaping the community structures.
Article
Environmental Sciences
Preeyaporn Pornkulwat, Eakalak Khan, Sorawit Powtongsook, Wuttichai Mhuantong, Chamorn Chawengkijwanich, Tawan Limpiyakorn
Summary: This study comprehensively investigated the impacts of ammonia and salinity on culturing nitrifying microorganisms and successfully formulated nitrifying cultures for different recirculating aquaculture system (RAS) types. The findings suggest that cultures prepared at low ammonia loading rates are most suitable for various RAS types, while cultures prepared at high ammonia loading rates are dominated by nitrifying microorganisms with low substrate affinity.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Soil Science
Haiyang Liu, Hangwei Hu, Xing Huang, Tida Ge, Yongfu Li, Zhenke Zhu, Xingmei Liu, Wenfeng Tan, Zhongjun Jia, Hongjie Di, Jianming Xu, Yong Li
Summary: Chemoautotrophic canonical ammonia-oxidizers play a significant role in autotrophic nitrification during the mineralization of organic substances with low C/N ratios in paddy soils, as shown by N-15 tracing and DNA-SIP techniques.
SOIL BIOLOGY & BIOCHEMISTRY
(2021)
Article
Microbiology
Jonathan Rodriguez, Seemanti Chakrabarti, Eunkyung Choi, Nisreen Shehadeh, Samantha Sierra-Martinez, Jun Zhao, Willm Martens-Habbena
Summary: The discovery of ammonia-oxidizing archaea (AOA) and complete ammonia-oxidizing (comammox) bacteria in terrestrial ecosystems highlights their important role in nitrification. A nutrient-limited enrichment strategy was found to favor the growth of comammox bacteria with high affinity and yield. Direct enrichment from soil and nutrient-limited sub-cultivation consistently yielded high enrichments of Nitrosocosmicus-affiliated AOA associated with multiple canonical nitrite-oxidizing Nitrospira strains.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Soil Science
Yongxin Lin, Hang-Wei Hu, Guiping Ye, Jianbo Fan, Weixin Ding, Zi-Yang He, Yong Zheng, Ji-Zheng He
Summary: The meta-analysis of laboratory incubation studies with 1-octyne as the nitrification inhibitor shows that AOB play a more dominant role in soil nitrification in cropland than in wetland soils, indicating a major role of AOB in acidic soil nitrification. Moreover, the reduction of nitrification rate by 1-octyne is greater in soils incubated in slurry and with nitrogen additions, suggesting AOB's importance in nitrification in global acidic soils.
Article
Ecology
Franziska Klotz, Katharina Kitzinger, David Kamanda Ngugi, Petra Busing, Sten Littmann, Marcel M. M. Kuypers, Bernhard Schink, Michael Pester
Summary: Deep oligotrophic lakes sustain large populations of Nitrososphaeria, which are the key ammonia oxidizers in this habitat. However, their impact on N-cycling in lakes has rarely been quantified. This study followed the Nitrososphaeria population in Lake Constance and demonstrated their important role in the nitrogen cycle of deep oligotrophic lakes.
Article
Biotechnology & Applied Microbiology
Elizabeth French, Jessica A. Kozlowski, Annette Bollmann
Summary: The study found that ammonia-oxidizing archaea are more abundant in systems with low ammonium availabilities, while ammonia-oxidizing bacteria are more abundant when ammonium availability increases. Population dynamics of ammonia oxidizers may shift in response to changes in ammonium concentrations.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Review
Engineering, Environmental
Shaoyi Xu, Xiaolong Wu, Huijie Lu
Summary: This review discusses the roles of nitrogen-cycling microorganisms in wastewater treatment, focusing on often overlooked microbes that are functionally important. Advancements in molecular technologies have enabled comprehensive studies on the application and potential of these microbes in nitrogen removal processes in wastewater treatment plants.
FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING
(2021)
Article
Soil Science
Zhen Zhen, Gaoyang Li, Yijie Chen, Ting Wei, Huijun Li, Fengcheng Huang, Yongxiang Huang, Lei Ren, Yanqiu Liang, Dayi Zhang, Zhong Lin, Zhe Li
Summary: Plantations of saline-alkali tolerant rice in coastal areas are proposed to improve rice yield and ensure food security. This study investigated the effects of saline-alkali tolerant rice cultivation on the nitrification process in coastal solonchaks. The results showed that the abundance and nitrification rates of AOA and AOB decreased with increasing salinity, but increased significantly in the rice rhizosphere. Saline-alkali tolerant rice cultivation enhanced the nitrification process of the soil by increasing the nitrification contribution of AOB.
APPLIED SOIL ECOLOGY
(2023)
Article
Engineering, Environmental
Carlos Domingo-Felez, Barth F. Smets
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2020)
Article
Agricultural Engineering
Borja Valverde-Perez, Wei Xing, August A. Zachariae, Monika M. Skadborg, Astrid F. Kjeldgaard, Alejandro Palomo, Barth F. Smets
BIORESOURCE TECHNOLOGY
(2020)
Article
Environmental Sciences
Camilla Tang, Berit Godskesen, Henrik Aktor, Marlies van Rijn, John B. Kristensen, Per S. Rosshaug, Hans-Jorgen Albrechtsen, Martin Rygaard
Summary: The calculation of CCPP shows fluctuations at different temperatures and in open systems, indicating that all relevant ionic species should be considered to avoid underestimating the potential for CaCO3 precipitation in low temperature and closed systems.
Article
Biology
Robert Niederdorfer, Damian Hausherr, Alejandro Palomo, Jing Wei, Paul Magyar, Barth F. Smets, Adriano Joss, Helmut Buergmann
Summary: The research found that anaerobic ammonium oxidizing (anammox) bacteria are highly sensitive to fluctuations in oxygen concentration and temperature under mainstream wastewater treatment conditions, leading to inhibition that affects the efficiency of nitrogen removal. Genomic analysis showed that changes in temperature and oxygen concentration resulted in alterations in gene expression levels within microbial communities, affecting the nitrogen removal process.
COMMUNICATIONS BIOLOGY
(2021)
Review
Engineering, Environmental
Qingxian Su, Anna-Ricarda Schittich, Marlene Mark Jensen, Howyong Ng, Barth F. Smets
Summary: Organic micropollutants (OMPs) can be biotransformed by ammonia-oxidizing bacteria through direct and indirect enzymatic reactions, primarily via hydroxylation and deamination. Ammonia-oxidizing bacteria play a key role in OMP biotransformation during wastewater treatment processes, especially through the activity of the ammonia monooxygenase enzyme.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Engineering, Environmental
Shelesh Agrawal, David G. Weissbrodt, Medini Annavajhala, Marlene Mark Jensen, Jose Maria Carvajal Arroyo, George Wells, Kartik Chandran, Siegfried E. Vlaeminck, Akihiko Terada, Barth F. Smets, Susanne Lackner
Summary: A baseline investigation of qPCR-based analysis for microbial communities in PN/A systems revealed significant variability in DNA extraction and primer efficiency among different laboratories, as well as varying differences in abundance data for different microbial guilds. These insights will provide guidance for interpreting qPCR data for environmental scientists, engineers, and treatment plant operators.
Article
Agricultural Engineering
Jinyu Ye, Huan Gao, Carlos Domingo-F, Junkang Wu, Manjun Zhan, Ran Yu, Barth F. Smets
Summary: Chronic exposure to zinc oxide nanoparticles in a sequencing batch reactor has shown dose-dependent depression effects on ammonia nitrogen and total nitrogen removal efficiencies as well as N2O emissions. The suppressed N2O emissions were positively correlated with the activity ratios of nitrite/NO reductases and N2O reductase.
BIORESOURCE TECHNOLOGY
(2021)
Article
Engineering, Environmental
Liguan Li, Joseph Nesme, Marcos Quintela-Baluja, Sabela Balboa, Syed Hashsham, Maggie R. Williams, Zhuofeng Yu, Soren J. Sorensen, David W. Graham, Jesus L. Romalde, Arnaud Dechesne, Barth F. Smets
Summary: The study of urban wastewater systems in Denmark, Spain, and the UK revealed a significant reduction in antibiotic resistance genes during sewage conveyance, presenting opportunities for antibiotic resistance management and caution for surveillance based on sewage.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Engineering, Environmental
Ling Yuan, Yubo Wang, Lu Zhang, Alejandro Palomo, Jizhong Zhou, Barth F. Smets, Helmut Burgmann, Feng Ju
Summary: A genome-centric quantitative metatranscriptomic approach was used to analyze bacterial hosts of antibiotic resistance genes (ARGs) in urban wastewater treatment plants, revealing widespread presence of ARGs against multiple classes of antibiotics in various bacterial hosts, including potential pathogens and indigenous denitrifying bacteria.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Engineering, Environmental
Toshikazu Suenaga, Takumi Ota, Kohei Oba, Kentaro Usui, Toshiki Sako, Tomoyuki Hori, Shohei Riya, Masaaki Hosomi, Kartik Chandran, Susanne Lackner, Barth F. Smets, Akihiko Terada
Summary: This study investigated the phylogeny of resident N2O-reducing bacteria in an anammox microbial community and quantified individually the processes of N2O production and consumption. The results showed that N2O-reducing bacteria primarily belonged to Burkholderiaceae and Chloroflexus genera, and other less abundant strains in Rhodoferax, Azospirillum, Lautropia, and Bdellovibrio also likely acted as N2O sinks. The study also introduced a novel N-15 tracer method to quantify N2O production and consumption rates, providing insights into the potential N2O-reducing activity in anammox-based processes.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Engineering, Environmental
Camilla Tang, Martin Rygaard, Per S. Rosshaug, John B. Kristensen, Hans-Jorgen Albrechtsen
Summary: Drinking water softening is aimed at increasing consumer convenience by reducing lime scaling and soap use, but it also alters the mineral composition of water, impacting health and corrosion. Different softening technologies have varying effects on water quality indicators, with none scoring positive on all aspects.
Article
Environmental Sciences
Shaoyi Xu, Wenbo Chai, Rui Xiao, Barth F. Smets, Alejandro Palomo, Huijie Lu
Summary: This study identified Candidatus Nitrospira nitrosa-like comammox bacteria as the predominant ammonia oxidizers in a lab-scale BNR system, with the potential to use acetate and propionate, stimulate ammonia oxidation rate, and interact metabolically with heterotrophs. Comammox bacteria have shown to provide vitamins/cofactors to heterotrophs and receive amino acids in return, contributing to their survival in the system and outcompeting ammonia oxidizing bacteria. Systems with comammox bacteria as the major nitrifiers have great potential for superior performance with low aeration cost and N2O emission at full-scale plants.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Public, Environmental & Occupational Health
Nasreen Hassoun-Kheir, Yoav Stabholz, Jan-Ulrich Kreft, Roberto de la Cruz, Arnaud Dechesne, Barth F. Smets, Jesus L. Romalde, Alberto Lema, Sabela Balboa, Carlos Garcia-Riestra, Eva Torres-Sangiao, Ami Neuberger, David Graham, Marcos Quintela-Baluja, Dov J. Stekel, Jay Graham, Amy Pruden, Joseph Nesme, Soren Johannes Sorensen, Rupert Hough, Mical Paul
Summary: By utilizing a systematic review and Delphi consensus method, the EMBRACE-WATERS statement was developed as a checklist of recommendations for reporting studies of AMR in wastewater and related aquatic environments. The consensus among a multidisciplinary panel of experts resulted in 21 items comprising essential elements for high-quality reporting, with explanations and reporting examples provided for each item. The statement aims to improve reporting quality, facilitate communication among researchers, and guide peer-reviewers and editors in evaluating manuscripts in the field of AMR research in aquatic environments.
Article
Engineering, Environmental
Tomas Diera, Anne Holm Thomsen, Selina Tisler, Lone Tolstrup Karlby, Peter Christensen, Per Sand Rosshaug, Hans-Jurgen Albrechtsen, Jan H. Christensen
Summary: This study aimed to identify potential organic compounds migrating from HDPE pipes used to distribute drinking water. A total of 133 compounds originating from the PE pipes were detected, with 31 compounds detected by suspect screening and the remaining 102 compounds detected by non-target screening. Additionally, a larger proportion of the compounds detected were assigned to rubber seals, which may lead to a reconsideration of their role in drinking water systems.
Article
Agricultural Engineering
Sike Wang, Kento Ishii, Heng Yu, Xuchuan Shi, Barth F. Smets, Alejandro Palomo, Jiane Zuo
Summary: The study found that rapid temperature drops had impacts on the performance of the anammox process and the metabolism of core microbial populations, with the metabolism of AnAOB responding more significantly to low temperatures compared to heterotrophs.
BIORESOURCE TECHNOLOGY
(2021)
Article
Engineering, Environmental
Rong-Gui Zhu, Chang-Gui Pan, Feng-Jiao Peng, Chao-Yang Zhou, Jun-Jie Hu, Kefu Yu
Summary: This comprehensive survey investigated the occurrence, bioaccumulation, and trophic magnification of parabens and their metabolite 4-HB in a marine food web. Results showed that parabens were the predominant pollutants in marine organisms, with significant bioaccumulation from sediments. The estimated trophic magnification factor indicated biomagnification for MeP and trophic dilution for 4-HB. Overall, the risks for humans consuming marine organisms were found to be low.
Article
Engineering, Environmental
Andres F. Torres-Franco, Deborah Leroy-Freitas, Cristina Martinez-Fraile, Elisa Rodriguez, Pedro A. Garcia-Encina, Raul Munoz
Summary: Anaerobic and microalgae-based technologies have emerged as sustainable alternatives for municipal wastewater treatment. However, the presence of viruses in the treated wastewater is a major concern for reuse applications. This study assessed the ability of these technologies to reduce viruses during secondary wastewater treatment. The results showed that all technologies were effective in reducing the concentration of viruses, with microalgae-based treatment exhibiting the highest potential for reducing the disinfection requirements of treated wastewater.
Article
Engineering, Environmental
Young Gwang Kim, Sae Yun Kwon, Spencer J. Washburn, Scott C. Brooks, Ji Won Yoon, Lucien Besnard
Summary: The study uses Hg isotope ratios to identify the sources and exposure pathways of mercury in bivalves, finding that dissolved Hg phases in the water column are the primary source and exposure pathway to bivalves. This provides new insights into using bivalves as bioindicators for sediment quality monitoring.
Article
Engineering, Environmental
Hui Geng, Ying Xu, Rui Liu, Dianhai Yang, Xiaohu Dai
Summary: This study investigates the effect of cation exchange resin (CER) on the sequential recovery of hydrogen and methane from anaerobic digestion (AD) and the corresponding mechanisms. The results show that CER can simultaneously enhance the production of hydrogen and methane by promoting the solubilisation, hydrolysis, and acidification of organic matter. Additionally, CER facilitates effective contact between bacteria and organic particulates and reduces the energy barrier for mass transfer during methane production. The study also reveals changes in the microbial community structure and metagenomics during the AD process.
Article
Engineering, Environmental
Xiaojing Lin, Zhan Jin, Shunfeng Jiang, Zhiquan Wang, Suqing Wu, Ke Bei, Min Zhao, Xiangyong Zheng
Summary: Dehumidification combined with addition of absorbent resin supplement (ARS) was used to achieve rapid evaporation of non-pretreated urine, resulting in high water evaporation efficiency and nutrient recovery.
Article
Engineering, Environmental
Yangli Che, Chaoran Lin, Shen Li, Jiao Liu, Longhai Zhu, Shilei Yu, Nan Wang, Haoshuai Li, Mutai Bao, Yang Zhou, Tonghao Si, Rui Bao
Summary: Hydrodynamic processes play a crucial role in the transmission of sediments, microbial assembly, and organic carbon redistribution in the ocean. Through experiments and analysis, we found that hydrodynamics shape the assembly of microbial communities and control the redistribution of different sourced organic carbon, thereby influencing microbial-mediated biogeochemical transformation.
Article
Engineering, Environmental
Chao Chen, Yu Yang, Nigel J. D. Graham, Zhenyu Li, Xingtao Yang, Zhining Wang, Nadia Farhat, Johannes S. Vrouwenvelder, Li -an Hou
Summary: The fouling of seawater reverse osmosis membranes is a persistent challenge in desalination. This study monitored the operational performance of a desalination plant for 7 years and the fouling development in different areas of membrane modules. The findings showed that operational performance declined over time and fouling mainly occurred at the feed side of the modules, with the highest microbial diversity. Keystone species like Chloroflexi and Planctomycetes played an important role in maintaining community structure and biofilm maturation. Polysaccharides, soluble microbial products, marine humic acid-like substances, and inorganic substances contributed to fouling. Overall, biofouling had a significant impact on membrane fouling after 7 years of operation.
Article
Engineering, Environmental
Dan Li, Jieyi Sun, Yibo Fu, Wentao Hong, Heli Wang, Qian Yang, Junhong Wu, Sen Yang, Jianhui Xu, Yunfei Zhang, Yirong Deng, Yin Zhong, Ping'an Peng
Summary: Sulfidation-oxidation treatment of magnetite (Fe3O4) enhances the production of dark center dot OH, which can efficiently degrade dissolved organic matter (DOM) and accelerate carbon cycling.
Article
Engineering, Environmental
Cheng Yu, Kaijun Wang, Kaiyuan Zhang, Ruiyang Liu, Pingping Zheng
Summary: This study implemented a microaerobic-aerobic configuration in a full-scale municipal wastewater treatment facility and investigated the effects on sludge characteristics, pollutant removal, microbial community, and granulation mechanisms. The results showed successful transition from flocculent-activated sludge to well-defined AGS after two months of operation. The primary pathways for pollutant removal were simultaneous nitrification, denitrification, and phosphorus removal. Moreover, the incorporation of internal separators induced shifts in the flow pattern, which promoted granulation.
Article
Engineering, Environmental
Zhe Zhang, Shaoyang Hu, Guangrong Sun, Wei Wang
Summary: Halogenated aromatic disinfection byproducts (DBPs), such as halogenated phenols, have garnered widespread attention due to their high toxicity and prevalence. This study reports on the analysis, occurrence, and cytotoxicity of a group of emerging halogenated aromatic DBPs, known as halogenated polyhydroxyphenols (HPPs), in drinking water.
Article
Engineering, Environmental
Shengyue Chen, Jinliang Huang, Peng Wang, Xi Tang, Zhenyu Zhang
Summary: Accurate prediction of river water quality is crucial for sustainable water management. This study introduces wavelet analysis and transfer learning techniques to assist LSTM modeling, proposing a newly coupled modeling approach that improves short-term prediction of river water quality.
Article
Engineering, Environmental
Bang Du, Xinmin Zhan, Piet N. L. Lens, Yifeng Zhang, Guangxue Wu
Summary: Efficient anaerobic digestion relies on the cooperation of different microorganisms with different metabolic pathways. This study investigated the effects of different operational modes and the addition of powdered activated carbon (PAC) on ethanol metabolic pathways. The results showed that the SBR mode and the presence of CO2 facilitated ethanol metabolism towards propionate production, while the CFR mode with extended solids retention time enriched Geobacter. Adjusting operational modes and PAC addition can modulate anaerobic ethanol metabolism and enrich Geobacter.
Article
Engineering, Environmental
Wanfa Wang, Si-Liang Li, Jun Zhong, Yuanbi Yi, Fujun Yue, Zenglei Han, Qixin Wu, Ding He, Cong-Qiang Liu
Summary: This study compares the carbon biogeochemical processes in karst and non-karst regions within large thermal stratified river-reservoir systems. The results demonstrate that karst reservoirs have a reduced potential for carbon emissions and highlight the importance of considering geologic settings to improve accuracy in regional and global CO2 emission estimates.
Article
Engineering, Environmental
Chunxia Jiang, Zelong Zhao, Dong Zhu, Xiong Pan, Yuyi Yang
Summary: This study analyzed the occurrence and distribution of antibiotic resistance genes (ARGs) in different environmental media of the Yangtze River using metagenomics. Core resistome dominated by multidrug resistance genes was found in all samples, while rare resistome dominated by various resistance genes was more prevalent in plasmids. Specific bacteria were identified as hosts for both core and rare resistomes, with high clinical concern ARGs found in the rare resistome. Particle-associated environment provided the most ideal conditions for resistome hosts. This study provided insights into the genetic locations of ARGs and the community assembly mechanisms of ARG hosts in freshwater environments.
Article
Engineering, Environmental
Yu Zhang, Yongtao He, Linchun Jia, Lei Xu, Zheng Wang, Yueling He, Ling Xiong, Xumeng Lin, Hong Chen, Gang Xue
Summary: By synergizing organic carbon source, thiosulfate, and zero-valent iron, efficient mixotrophic denitrification of oligotrophic secondary effluent can be achieved. Thiosulfate plays a vital role in promoting TN removal efficiency, while corrosion of Fe0 releases OH- to neutralize H+ from thiosulfate-driven denitrification, creating a suitable environment for denitrification. The coordination of thiosulfate and Fe0 maintains the dominance of Thiobacillus for denitrification.