期刊
FRONTIERS IN PLANT SCIENCE
卷 6, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2015.00430
关键词
phosphoproteomics; enrichment; quantification; phosphorylation site mapping; plant biology
资金
- National Science Foundation of China [31471552, 31401441]
- National Science Foundation of Heilongjiang Province [C201202]
- Common College Science and Technology Innovation Team of Heilongjiang Province
Protein phosphorylation has long been recognized as an essential mechanism to regulate many important processes of plant life. However, studies on phosphorylation mediated signaling events in plants are challenged with low stoichiometry and dynamic nature of phosphorylated proteins. Significant advances in mass spectrometry based phosphoproteomics have taken place in recent decade, including phosphoprotein/phosphopeptide enrichment, detection and quantification, and phosphorylation site localization. This review describes a variety of separation and enrichment methods for phosphoproteins and phosphopeptides, the applications of technological innovations in plant phosphoproteomics, and highlights significant achievement of phosphoproteomics in the areas of plant signal transduction, growth and development.
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