4.8 Article

Characterization of extracellular polymeric substances produced by mixed microorganisms in activated sludge with gel-permeating chromatography, excitation-emission matrix fluorescence spectroscopy measurement and kinetic modeling

期刊

WATER RESEARCH
卷 43, 期 5, 页码 1350-1358

出版社

PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.watres.2008.12.004

关键词

Activated sludge; Characterization; Excitation-emission matrix (EEM); Extracellular polymeric substances (EPS); Gel-permeating chromatography (GPC); Kinetic modeling

资金

  1. Natural Science Foundation of China [20577048, 50625825, 50738006, 50878203]

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In this work the extracellular polymeric substances (EPS) produced by mixed microbial community in activated sludge are characterized using gel-permeating chromatography (GPC), 3-dimensional excitation-emission matrix (EEM) fluorescence spectroscopy measurement and mathematical modeling. Chromatograms of extracted EPS exhibit seven peaks, among which proteins have four peaks and polysaccharides have three peaks. Evolution of the chromatogram area indicates that the quantity of produced EPS increases significantly in the substrate utilization process. With the parallel factor analysis (PAR-AFAC) approach, two components of the polymer matrix are identified by the EEM analysis, one as EPS proteins at Ex/Em 280/340 nm and one matrix associated as fulvic-acid-like substances at 320/400 nm. The proteins and fulvic-acid-like substances in the EPS increase in the substrate utilization phase, but decrease in the endogenous phase. To have a better insight into EPS production, the kinetic modeling of EPS is performed with regard to their molecular weight distribution and chemical natures identified by GPC and EEM. In this way, the dynamics of these important microbial products are better understood. (C) 2008 Elsevier Ltd. All rights reserved.

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