Article
Medical Laboratory Technology
Jordi Farre-Segura, Neus Fabregat-Cabello, Chiara Calaprice, Laurent Nyssen, Stephanie Peeters, Caroline Le Goff, Etienne Cavalier
Summary: A rapid and specific LC-MS/MS method for glucagon quantitation has been developed and validated, showing stability and accuracy in comparison to other immunoassay methods. This method could potentially establish a standardized approach for glucagon analysis in routine care settings.
CLINICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Prasad Phapale, Andrew Palmer, Rose Muthoni Gathungu, Dipali Kale, Britta Brugger, Theodore Alexandrov
Summary: EMBL-MCF is an open LC-MS/MS spectral library with over 1600 fragmentation spectra from 435 authentic standards of endogenous metabolites and lipids. It features chromatographic profiles acquired with different LC-MS methods and coverage of different adduct ions. The library contains many biologically important metabolites and unique metabolites and lipids compared to other public libraries.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Food Science & Technology
Xiu Yuan, Chang Jo Kim, Raekeun Lee, Min Kim, Hee Jeong Shin, Leesun Kim, Won Tae Jeong, Yongho Shin, Kee Sung Kyung, Hyun Ho Noh
Summary: This study optimized a method for the multi-residue analysis of 287 pesticide residues in mandarin orange and grapefruit using the quick, easy, cheap, effective, rugged, and safe (QuEChERS) technique combined with liquid chromatography-tandem mass spectrometry. The method was validated in terms of selectivity, LOQ, linearity, accuracy, precision, and matrix effect, showing satisfactory results for routine laboratory testing of citrus fruits for pesticide residue analyses.
Article
Chemistry, Analytical
Sakshi Gautam, Alireza Banazadeh, Byeong Gwan Cho, Mona Goli, Jieqiang Zhong, Yehia Mechref
Summary: Researchers successfully achieved efficient separation of glycan isomers using mesoporous graphitized carbon (MGC) material in a 1 cm-long column, showing reproducibility and stability in both capillary columns and packed nanospray emitters.
ANALYTICAL CHEMISTRY
(2021)
Review
Biochemical Research Methods
Daniella Rheingantz Decker Soares, Marina Venzon Antunes, Rafael Linden
Summary: The most commonly employed analytical approaches for determining cortisol concentrations in hair include simple methanolic extraction, reversed-phase separation, and MRM detection in negative ion mode. The reported assays have acceptable sensitivity for clinical purposes. The increasing use of mass spectrometry in clinical laboratories may contribute to the establishment of LC-MS/MS as the method of choice for the determination of cortisol concentrations in hair.
Article
Biochemical Research Methods
Fadumo Ahmed Isse, Tyson Le, Sherif Hanafy Mahmoud
Summary: Nimodipine is a dihydropyridine calcium channel blocker used to improve outcomes and prevent cerebral ischemia in patients with aneurysmal subarachnoid hemorrhage. A new LC-MS/MS method was presented to quantify nimodipine enantiomers in human plasma, showing potential application in pharmacological and pharmacokinetic studies.
BIOMEDICAL CHROMATOGRAPHY
(2021)
Article
Biochemical Research Methods
Fatemeh Aghai, Sebastian Zimmermann, Max Kurlbaum, Pius Jung, Theo Pelzer, Hartwig Klinker, Nora Isberner, Oliver Scherf-Clavel
Summary: A liquid chromatography tandem mass spectrometry method has been developed and validated for the analysis of ten kinase inhibitors in human serum and plasma, meeting the validation guidelines of regulatory agencies. The method allows for rapid and sensitive monitoring of kinase inhibitor concentrations in patients.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Applied
Pierre Guichard, Michel Laurentie, Dominique Hurtaud-Pessel, Eric Verdon
Summary: An LC-MS/MS method was developed for confirmation of nitrofuran metabolites in meat and aquaculture products, including DNSH. The method showed good trueness and precision, with decision limits and detection limits well within the specified criteria. The sample preparation included a washing step to analyze only protein-bound residues.
Article
Chemistry, Analytical
Lauren M. Bishop, Tong Shen, Oliver Fiehn
Summary: Accurate and reproducible quantification of endogenous lipids is crucial for large-scale lipidomic analyses. Many lipids in liquid chromatography-electrospray ionization-mass spectrometry form various adducts with buffer components, and the mechanisms and conditions affecting adduct formation are poorly understood. Variations in adduct ratios can lead to significant inaccuracies during quantification, and the use of multiple adduct ions improves accuracy compared to using a single adduct ion.
ANALYTICAL CHEMISTRY
(2023)
Article
Environmental Sciences
Seunghwa Lee, Kang Mi Lee, Sang Moon Han, Hyeon-Jeong Lee, Changmin Sung, Hophil Min, Hosub Im, Sang Beom Han, Sangwon Cha, Jaeick Lee
Summary: To assess the combined effects of environmental pollutants (EPOLs), a comprehensive analytical method was developed to simultaneously analyze multiple EPOLs. The method overcame technical challenges associated with enzyme hydrolysis, simultaneous extraction, and analysis. The method was validated and showed potential for long-term, large-scale, and data-rich human biomonitoring of EPOLs.
ENVIRONMENTAL RESEARCH
(2022)
Article
Chemistry, Analytical
Tobias Schlappack, Matthias Rainer, Nikolaus Weinberger, Gunther K. Bonn
Summary: This study describes the use of modified halloysite nanotubes as a novel solid material for the efficient solid phase extraction of toxic pyrrolizidine alkaloids. The optimized method showed high extraction efficiencies and overall recoveries for a mixture of pyrrolizidine alkaloids using UHPLC-MS/MS analysis. The novel solid phase material exhibited high selectivity and competitiveness compared to a commercial procedure.
ANALYTICAL METHODS
(2022)
Article
Chemistry, Inorganic & Nuclear
Alireza Sedrpoushan, Hoda Haghi, Mohammad Sohrabi
Summary: This study focused on the chemistry of Diploschistes diacapsis using liquid chromatography electrospray ionization tandem mass spectrometry. Seven compounds from D. diacapsis lichen species were discovered and analyzed. The maceration method proved effective for lichen extraction. The findings can contribute to flora data and improve mass spectrometry library information, providing a foundation for biological and pharmacological studies.
INORGANIC CHEMISTRY COMMUNICATIONS
(2022)
Article
Medical Laboratory Technology
Lizi Jin, Youli Lu, Xilian Yi, Meiwei Zhang, Jiangtao Zhang, Weiyan Zhou, Jie Zeng, Tianjiao Zhang, Chuanbao Zhang
Summary: This study compared the differences between different methods for measuring serum folate and found significant variations among the assays. Although there was good correlation between the assays, there was a lack of agreement. Therefore, it is important to routinely assess the accuracy and consistency of serum folate assays and promote standardization efforts.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2022)
Article
Food Science & Technology
Marica Erminia Schiano, Federica Sodano, Chiara Cassiano, Ferdinando Fiorino, Serenella Seccia, Maria Grazia Rimoli, Stefania Albrizio
Summary: The consumption of plant-based beverages as an alternative to cow's milk has gained attention worldwide. This study focused on monitoring the intake of BPA, BPB, and BPS in the Italian population through the consumption of these beverages. Analysis of popular brands in Italy found BPA and BPB in the samples. Risk assessment showed that if the new temporary TDI value is confirmed, more attention would be needed to protect consumer health.
Article
Biochemical Research Methods
Steven J. Lehotay
Summary: UHPLC coupled with MS/MS is a powerful tool for multiclass, multiresidue analysis. The study validated quantification and identification of 169 veterinary drugs in eggs, recommending monitoring >2 ion transitions per analyte for improved analysis with MS/MS.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
