Article
Biochemistry & Molecular Biology
Vladimir I. Timofeev, Ilya V. Fateev, Maria A. Kostromina, Yuliya A. Abramchik, Irina D. Konstantinova, Vladimir V. Volkov, Dmitry D. Lykoshin, Olga O. Mikheeva, Tatiana I. Muravieva, Roman S. Esipov, Inna P. Kuranova
Summary: Two recombinant purine nucleoside phosphorylases from Thermus thermophilus HB27 were purified and characterized. TthPNPI is specific to guanosine while TthPNPII is specific to adenosine. The oligomeric structures of these enzymes, as well as the key residues in their active sites, were analyzed. The crystal structure of TthPNPII was determined and its active site cavity was examined. The hexameric structure of TthPNPII, which is different from other purine nucleoside phosphorylases, was observed for the first time.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Biochemistry & Molecular Biology
Felix Kaspar, Peter Neubauer, Anke Kurreck
Summary: The pyrimidine nucleoside phosphorylase from Thermus thermophilus shows remarkable activity and stability over a wide range of pH, temperature, and cosolvent space. However, its use as a biocatalyst is limited due to inhibition by nucleobases at low concentrations.
Article
Chemistry, Multidisciplinary
Alibek Kruglikov, Yulong Wei, Xuhua Xia
Summary: Majority of protein structure studies use model organisms like E. coli as expression systems. However, differences in cellular environment factors, particularly temperature and chaperones, can cause improper folding of native proteins in extremophiles. This study compares the effects of using E. coli as expression system on protein structures between mesophilic bacteria and a thermophile T. thermophilus, and finds significant differences, suggesting the need for unique protein expression systems in protein folding studies of extremophiles.
Review
Chemistry, Multidisciplinary
Rodrigo Diaz-Espinoza
Summary: Peptides and proteins can form highly ordered amyloid structures that have unique mechanical properties and catalytic activities. Recent advances in the design of amyloids have focused on the development of catalytic activities, such as hydrolysis, as well as novel types of activities. These catalytically active amyloids have great potential for the development of future bionanomaterials aimed at specific applications.
Article
Cell Biology
Kim Shortall, Edel Durack, Edmond Magner, Tewfik Soulimane
Summary: The thermophilic ALDH(Tt) has been found to possess dual enzymatic activity and broad substrate specificity, showing activity for a range of aldehydes and ester substrates. However, it may exhibit differing levels of activity towards specific substrates with para-substituted benzaldehydes being efficiently processed while ortho-substituted ones show no catalytic activity.
Article
Plant Sciences
Leidy Rusinque, Maria Joao Camacho, Clara Serra, Filomena Nobrega, Maria L. Inacio
Summary: Meloidogyne spp., commonly known as the root-knot nematodes (RKNs), are highly damaging plant parasitic nematodes that cause root galls and significant yield losses. A survey conducted in Portugal from 2017 to 2022 identified the presence of various Meloidogyne species across different regions, with Meloidogyne incognita and M. javanica being the most prevalent species. Morphological and biochemical characterization confirmed the accurate identification of RKNs and provided updated information on their distribution in Europe. This study contributes to the development of management strategies for RKNs.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Xiaoyu Liu, Mingyang Zhou, Shu Xing, Tao Wu, Hailun He, John Kevin Bielicki, Jianbin Chen
Summary: Est19 is a novel hormone-sensitive lipase (HSL) family esterase with a conserved catalytic triad structure, exhibiting high catalytic efficiency towards the substrate pNPC6. Mutation experiment showed that replacing the adjacent glutamic acid to the critical catalytic serine significantly reduces the activity and efficiency of the enzyme.
Review
Biochemistry & Molecular Biology
Adamu Idris Matinja, Nor Hafizah Ahmad Kamarudin, Adam Thean Chor Leow, Siti Nurbaya Oslan, Mohd Shukuri Mohamad Ali
Summary: Microorganisms in cold environments use cold-active enzymes to adapt to low temperatures and perform efficient metabolism, which is crucial for industrial processes. However, the lack of complete understanding of the structure and cold adaptation mechanisms of cold-active enzymes hinders their industrial usage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Bo Zhuang, Lipsa Nag, Pierre Sournia, Anastasia Croitoru, Rivo Ramodiharilafy, Jean-Christophe Lambry, Hannu Myllykallio, Alexey Aleksandrov, Ursula Liebl, Marten H. Vos
Summary: Quenching of flavin fluorescence by electron transfer from neighboring aromatic residues plays a significant role in flavoproteins, and can be used as a probe for the active site configuration and dynamics. Modified amino acid residues in variants can lead to changes in the temporal characteristics, indicating heterogeneous configurations of electron donor-acceptor pairs.
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Lukas Wenger, Carsten P. Radtke, Eva Gerisch, Max Kollmann, Christof M. Niemeyer, Kersten S. Rabe, Juergen Hubbuch
Summary: This study compares the suitability and performance of different concentrations of low-melting agarose or agar-based inks in bioprinting. It was found that agarose bioinks with at least 4.5% polymer were the most suitable for printing biocatalytic reactors.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Lisanne Hameleers, Leena Penttinen, Martina Ikonen, Lea Jaillot, Regis Faure, Nicolas Terrapon, Peter J. Deuss, Nina Hakulinen, Emma R. Master, Edita Jurak
Summary: The study identified a novel bifunctional carbohydrate esterase (CE), BD-FAE, encoded within PULs of metagenomes from beaver droppings and moose rumen enriched on poplar hydrolysate. BD-FAE displayed feruloyl esterase (FAE) and acetyl esterase activity on synthetic and natural substrates, making it a new candidate for biomass processing.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Materials Science, Coatings & Films
Viktor Shapovalov, Anatoliy Zav'yalov, Aleksandra A. Meleshko (Morozova)
Summary: This study analyzes the discharge current-voltage characteristics of a magnetron with a hot titanium target in environments with reactive gases, showing practical applications for film deposition technologies.
SURFACE & COATINGS TECHNOLOGY
(2021)
Article
Biology
Rachel Knoener, Edward Evans, Jordan T. Becker, Mark Scalf, Bayleigh Benner, Nathan M. Sherer, Lloyd M. Smith
Summary: HIV-1 generates different classes of RNA that play distinct roles in viral replication, and understanding their interactions with host proteins is crucial. The HyPR-MSSV technique can isolate different types of HIV-1 RNA and identify their protein interactomes, revealing factors that impact HIV-1 gene expression.
Article
Biochemistry & Molecular Biology
Kurosh S. Mehershahi, Swaine L. Chen
Summary: The study revealed that three non-phasevariable Type I RMSs in Escherichia coli have no measurable impact on gene expression, in vivo virulence, or any of 1190 in vitro growth phenotypes. This suggests that there may be other nonregulatory methylation systems like these, expanding our understanding of the potential role of RMSs in bacterial evolution.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemical Research Methods
Josefin Ahlqvist, Javier A. Linares-Pasten, Maria Hakansson, Andrius Jasilionis, Karolina Kwiatkowska-Semrau, Olafur H. Friojonsson, Anna Karina Kaczorowska, Slawomir Dabrowski, Arnpor Aevarsson, Guomundur O. Hreggviosson, Salam Al-Karadaghi, Tadeusz Kaczorowski, Eva Nordberg Karlsson
Summary: This study describes the production, characterization, and structure determination of a novel Holliday junction-resolving enzyme called Hjc_15-6. The enzyme belongs to a group of archaeal enzymes and is capable of cleaving X-shaped dsDNA-Holliday junctions. The crystal structure of Hjc_15-6 was determined, and it represents a new fold for Holliday junction-resolving enzymes. It shows endonuclease activity towards Hjs and may form a dimer or a homodimer of dimers.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2022)