Article
Food Science & Technology
Ana Caroline de Oliveira, Vanete Thomaz-Soccol, Michelle Zibetti Tadra-Sfeir, Emanuel Maltempi de Souza, Maria Elena Crespo-Lopez, Herve Rogez
Summary: A new mRNA-based RT-qPCR technique was developed for rapid detection and quantification of viable Trypanosoma cruzi in acai fruits and juice, with a maximum recovery rate of 82.50% and a detection and quantification limit of 10 parasites/mL. The RT-qPCR efficiency was estimated at 97.27%, showing high accuracy in discriminating between viable and nonviable cells.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Food Science & Technology
Sunlin Luo, Ying Liu, Qi Guo, Xiong Wang, Ying Tian, Wenjun Yang, Juntao Li, Yiqiang Chen
Summary: In this study, a reliable and suitable GC-MS method was established for the determination of zearalenone and its derivatives in feed. The method involved sample purification using an immunoaffinity column, followed by silane derivatization and GC-MS analysis of target compounds. The method showed good linear relationship, low detection and quantification limits. Analysis of actual feed samples demonstrated the accuracy of the method for determining zearalenone and its derivatives.
Article
Chemistry, Applied
Jessica Cristina Zoratto Romoli, Milena Veronezi Silva, Giseli Cristina Pante, Danielle Hoeltgebaum, Juliana Cristina Castro, Gustavo Henrique Oliveira da Rocha, Isis Regina Grenier Capoci, Samuel Botiao Nerilo, Simone Aparecida Galerani Mossini, Eduardo Micotti da Gloria, Terezinha Inez Estivalet Svidzinski, Jane Martha Graton Mikcha, Miguel Machinski Junior
Summary: The study found that thyme and ginger essential oils showed the most potent inhibitory effects on DON and ZEA, making them potential alternatives to synthetic fungicides in the future.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2022)
Article
Biochemical Research Methods
Thomas Leichtfried, Helga Reisenzein, Siegrid Steinkellner, Richard A. Gottsberger
Summary: The study found that slight modifications of the reverse transcription step in the real-time PCR assay can significantly improve the detection limit, especially for certain plant viruses. This discovery could enhance the sensitivity in detecting viroids and benefit regulatory outcomes for national plant protection organizations.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Oncology
Ifra Mushtaq, Gh Rasool Bhat, Bilal Rah, Syed Besina, Sheikh Zahoor, Muneer A. Wani, Mubashir A. Shah, Sadaf Bashir, Muzamil Farooq, Rafiq A. Rather, Dil Afroze
Summary: Genetic instabilities and telomere dysfunction play a crucial role in cancer development, especially gastric cancer. This study evaluates the clinical implications of c-Myc, hTERT expression, and telomere length, shedding light on their significance in gastric cancer progression.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
M. Arizzi, S. Morra, G. Gilardi, M. Pugliese, M. L. Gullino, F. Valetti
Summary: The study demonstrates that utilizing autochthonous microbial consortia and high turnover rate hydrogenase genes can efficiently produce bio-hydrogen from green waste and low-value compost. Modulating the expression of hydrogenase genes can optimize dark fermentation process and enhance hydrogen production.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Materials Science, Ceramics
Sakthi Prasad, Margit Fabian, Anal Tarafder, Shashi Kant, Prasanta Kumar Sinha, Sucheta Tripathy, Annapurna Kalyandurg, Amarnath Reddy Allu, Kaushik Biswas
Summary: This study systematically evaluated the role of boron in regulating the degradation, apatite formation, and antibacterial properties of bioactive glasses. The results showed that the substitution of SiO2 with B2O3 led to changes in the structural composition of the glass, resulting in accelerated release of Si4+ and Ca2+ ions. Additionally, the borosilicate bioactive glasses exhibited excellent sintering and thermal processing characteristics.
CERAMICS INTERNATIONAL
(2022)
Article
Virology
Come J. Thieulent, Mariano Carossino, Laura Peak, Keith Strother, Wendy Wolfson, Udeni B. R. Balasuriya
Summary: Canine infectious respiratory disease complex (CIRDC) is the primary cause of respiratory disease in dogs, including SARS-CoV-2. To rapidly detect and differentiate SARS-CoV-2 from other respiratory pathogens from a public health perspective, researchers have developed a multiplex PCR panel that can detect and identify twelve pathogens associated with CIRDC, as well as three CIV subtypes.
Article
Cell Biology
Lingyi Wu, Sumin Wang, Bo Tang, Li Tang, Yuanyuan Lei, Yaojiang Liu, Min Yang, Guodong Yang, Dan Zhang, En Liu
Summary: This study revealed that the abnormal expression of hTERT and Gli1 in gastric cancer tissues is associated with invasion depth, lymph node metastasis, TNM stage, and distant metastasis. By regulating Gli1 expression, hTERT promotes invasion and metastasis of gastric cancer cells. Targeting the hTERT/Sp1/Gli1 axis may offer a new therapeutic strategy for gastric cancer.
JOURNAL OF MOLECULAR HISTOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zhidan Luo, Yong Xue, Xiaoyu Chen, Jian Zhang, Chen Lu
Summary: Researchers successfully constructed three variants of Tth pol with Mn2+-independent reverse transcriptase activity. Among them, the I640F/I709K variant showed good performance in one-step RT-qPCR.
PROTEIN AND PEPTIDE LETTERS
(2023)
Article
Biochemical Research Methods
Hasin Feroz, Daniel Cetnar, Robert Hewlett, Satish Sharma, Melissa Holstein, Sanchayita Ghose, Zheng Jian Li
Summary: Using a in-house RT-qPCR assay for selective detection of active o6 can be a faster, cheaper and safer alternative to third-party testing of BSL2 model viruses. This method can screen VI agents at only 5% of the cost compared to BSL2 virus testing, with a turnaround time of 2 to 3 days.
BIOTECHNOLOGY JOURNAL
(2021)
Article
Microbiology
Shuang Wu, Xiaolu Shi, Qiongcheng Chen, Yixiang Jiang, Le Zuo, Lei Wang, Min Jiang, Yiman Lin, Shisong Fang, Bo Peng, Weihua Wu, Hui Liu, Renli Zhang, Patrick S. L. Kwan, Qinghua Hu
Summary: This study compared the specificity and sensitivity of CPA and RT-qPCR kits for SARS-CoV-2 detection, providing a reference for clinical laboratories and informing testing protocols during the rapidly evolving COVID-19 pandemic. The CPA kit showed 100% sensitivity, specificity, PPV/NPV, while some RT-qPCR kits demonstrated good agreement with clinical diagnostic reports and good reproducibility. Differences between all kits were statistically significant.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2021)
Article
Immunology
Amir Abbas Hamidi, Mohammad Mahdi Forghanifard, Mehran Gholamin, Meysam Moghbeli, Bahram Memar, Ali Jangjoo, Mohammad Reza Motie, Fatemeh Molaei, Mohammad Reza Abbaszadegan
Summary: The study revealed that TWIST1 and MAML1 are widely expressed in gastric cancer patients, with a positive correlation between the two genes. Overexpression of MAML1 was associated with advanced tumor staging and nodal metastases, while TWIST1 expression was correlated with patient sex and advanced tumor grading.
MICROBIAL PATHOGENESIS
(2022)
Article
Virology
Lisa Dettinger, Crystal M. Gigante, Maria Sellard, Melanie Seiders, Puja Patel, Lillian A. Orciari, Pamela Yager, James Lute, Annette Regec, Yu Li, Dongxiang Xia
Summary: The Pennsylvania Department of Health Bureau of Laboratories tested 6855 animal samples for rabies using DFA and LN34 RT-qPCR, with a sensitivity of 0.03%. Two samples initially tested negative by DFA but were positive by LN34 RT-qPCR, which were confirmed positive upon re-testing. The levels of rabies virus in different brain structures varied, with higher abundance observed in more caudal brain structures, suggesting early infection.
Article
Oncology
Dikshat Gopal Gupta, Neelam Varma, Sarki Abba Abdulkadir, Sreejesh Sreedharanunni, Man Updesh Singh Sachdeva, Shano Naseem, Parveen Bose, Jogeshwar Binota, Pankaj Malhotra, Alka Khadwal, Amita Trehan, Subhash Varma
Summary: This study discovered a high incidence of Ph-like ALL cases in low- and middle-income countries and characterized their genetic alterations. A cost-effective multiplex panel of 11 overexpressed genes for the prompt detection of Ph-like ALL cases was proposed.
Article
Plant Sciences
V. S. Uma, Zeba Usmani, Minaxi Sharma, Deepti Diwan, Monika Sharma, Miao Guo, Maria G. Tuohy, Charalampos Makatsoris, Xiaobin Zhao, Vijay Kumar Thakur, Vijai Kumar Gupta
Summary: Algal biomass has great potential for sustainable production of various value-added compounds and products. Efficient valorization methods and biorefining channels are essential to enhance the commercial value of algal metabolites. Algal extracts are ideal sources of biotechnologically viable compounds. Emerging technologies in biomass valorization help reduce the cost burden of large-scale operations. Comprehensive extraction of multi-algal product biorefineries is envisioned to enhance the economic feasibility of algal products in the global market.
PHYTOCHEMISTRY REVIEWS
(2023)
Review
Biotechnology & Applied Microbiology
Liang Gong, Ajit Kumar Passari, Chunxiao Yin, Vijay Kumar Thakur, John Newbold, William Clark, Yueming Jiang, Shanmugam Kumar, Vijai Kumar Gupta
Summary: Nowadays, the increasing production, use, and disposal of plastic products is a major environmental issue. The presence of plastic particles and other impurities in the food supply chain poses significant health risks to humans, animals, and the environment. Biodegradable plastics aim to create a more sustainable and eco-friendly world. This article discusses the critical issues that need to be addressed for the commercial viability of bioplastic production.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Harshita Gaurav, Divyanshu Yadav, Ankita Maurya, Himanshu Yadav, Ramanand Yadav, Amritesh Chandra Shukla, Minaxi Sharma, Vijai Kumar Gupta, Javier Palazon
Summary: This review provides an overview of the traditional uses, botany, phytochemistry, and pharmacological advances of Withania somnifera L. Dunal (Ashwagandha), an important medicinal plant native to India. The plant contains a variety of primary and secondary metabolites, mainly steroidal compounds, steroidal lactones, alkaloids, and amino acids. In addition, it exhibits various medicinal activities such as anti-leukotriene, antineoplastic, analgesic, antioxidant, immunostimulatory, and rejuvenating properties. However, further research is needed to understand its medical applications.
Review
Chemistry, Physical
Kavya Dashora, Meghana Gattupalli, Gyan Datta Tripathi, Zoya Javed, Shweta Singh, Maria Tuohy, Prakash Kumar Sarangi, Deepti Diwan, Harikesh B. Singh, Vijai Kumar Gupta
Summary: This article discusses the lignin-degrading bacteria and fungi and their significance in breaking down lignocellulosic materials. Fungi, particularly white rot fungi, are considered the ideal microorganisms for lignin degradation due to their efficient lignolytic enzyme systems. The role of lignolytic enzymes such as laccases, lignin peroxidases, and manganese peroxidases in lignin depolymerization is emphasized in this article.
Article
Biotechnology & Applied Microbiology
Neha Srivastava, Rajeev Singh, Pardeep Singh, Irfan Ahmad, Ravindra Pratap Singh, Ashutosh Kumar Rai, Mohammed Asiri, Vijai Kumar Gupta
Summary: This article investigates the potential for economically viable and environmentally friendly biotransformation of lignocellulosic biomass (LCB) waste materials into cellulolytic enzymes and biofuels. The complexity of the structural composition of these wastes and the lack of a suitable bioprocess for their transformation are the main obstacles to their long-term viability. The article also evaluates the current constraints and successful limitations in the transformation process and proposes sustainable recommendations for improving the overall bioprocess on a sustainable scale. LCB waste valorization can contribute to the circular economy for sustainable future applications.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2023)
Review
Biotechnology & Applied Microbiology
Puttasiddaiah Rachitha, Krupashree Krishnaswamy, Renal Antoinette Lazar, Vijai Kumar Gupta, Baskaran Stephen Inbaraj, Vinay Basavegowda Raghavendra, Minaxi Sharma, Kandi Sridhar
Summary: The ayurvedic herb Emblica officinalis has great therapeutic and nutritional importance. It is used in Indian medicine for treating various illnesses and has been found to have hepatoprotective, immunomodulatory, antimicrobial, radioprotective, and hyperlipidemic effects. Nanoencapsulation technology can enhance the therapeutic activities and bioaccessibility of Emblica officinalis.
BIOENGINEERING-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Illia V. V. Kapitanov, Surya M. M. Sudheer, Toshikee Yadav, Kallol K. K. Ghosh, Nicholas Gathergood, Vijai K. K. Gupta, Yevgen Karpichev
Summary: The solubilization capacity of sustainable phenylalanine-derived surface-active ionic liquids (SAILs) towards polycyclic aromatic hydrocarbons (PAHs) was evaluated and compared with a conventional cationic surfactant, CTABr. The SAILs showed increased solubilization capacity with longer alkyl chain length, comparable to CTABr for certain SAIL types. The phenylalanine-derived SAILs also had the advantage of enzymatically cleaving ester and amide bonds under mild conditions for in situ separation of PAHs.
Review
Green & Sustainable Science & Technology
Prakash Kumar Sarangi, Akhilesh Kumar Singh, Rajesh Kumar Srivastava, Vijai Kumar Gupta
Summary: Globally, large quantities of agro-industrial wastes are generated annually in various industries, including milling, brewing, agriculture, and food. These wastes can be converted into valuable biochemicals/bioactive compounds. Pineapple by-products, which account for 60% of the fruit's weight, are currently disposed of as waste, leading to disposal and pollution issues. However, these by-products can be utilized for the production of biochemicals/bioactive compounds rich in cellulose and hemicellulose. This proposed review article focuses on waste-free approaches that add value, including innovative biorefinery techniques and microbial fermentation.
Article
Microbiology
Weizhe Sun, Quang D. Nguyen, Botond Kalman Suli, Firas Alarawi, Anett Szecsi, Vijai Kumar Gupta, Laszlo Ferenc Friedrich, Attila Gere, Erika Bujna
Summary: This study explores the microencapsulation technology for maintaining the viability of Lactiplantibacillus plantarum 299v strain. The results suggest that the core-to-wall ratios and polysaccharide ratios have an impact on the protection of the strain. The optimal microencapsulation formulation is a core-to-wall ratio of 1:1 and MD:RS ratio of 1:1, with fortification method, and storage at 4 degrees C.
Article
Green & Sustainable Science & Technology
Alireza Shafizadeh, Hossein Shahbeik, Mohammad Hossein Nadian, Vijai Kumar Gupta, Abdul-Sattar Nizami, Su Shiung Lam, Wanxi Peng, Junting Pan, Meisam Tabatabaei, Mortaza Aghbashlo
Summary: This study develops a machine learning-based research framework for modeling, understanding, and optimizing the catalytic steam reforming of volatile matter compounds. Chemical/ textural analyses are used to obtain input features, and ensemble machine learning provides the best prediction performance for toluene conversion and product distribution. The framework can expedite the search for optimal catalyst characteristics and reaction conditions.
JOURNAL OF CLEANER PRODUCTION
(2023)
Review
Green & Sustainable Science & Technology
Hossein Shahbeik, Alireza Shafizadeh, Vijai Kumar Gupta, Su Shiung Lam, Hajar Rastegari, Wanxi Peng, Junting Pan, Meisam Tabatabaei, Mortaza Aghbashlo
Summary: The unfavorable properties of biomass pyrolysis bio-oil require physical/thermochemical methods to enhance its quality. Nanotechnology offers potential solutions to overcome the drawbacks of conventional catalysts used in bio-oil upgrading. This article provides a comprehensive overview of the application of nanocatalysts in bio-oil upgrading, including their mechanisms and effects on important operating parameters. Nanocatalysts have been shown to yield higher bio-oil quality and have advantages over bulk catalysts. However, more research and development are needed before nanocatalysts can be fully realized in practical applications.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Green & Sustainable Science & Technology
Yadong Yang, Lila Yazdani, Mortaza Aghbashlo, Vijai Kumar Gupta, Junting Pan, Meisam Tabatabaei, Ahmad Rajaei
Summary: This study aimed to increase the commercial value of shrimp shell waste by using chitosan extracted from these wastes as a food emulsion stabilizer. The findings showed that the stabilizer developed from shrimp shell waste is suitable for stabilizing emulsions containing more polar oils with less degree of unsaturation.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Green & Sustainable Science & Technology
Sangita Dixit, Kalpana Sahoo, Mahendra Gaur, Rajesh Kumar Sahoo, Suchanda Dey, Vijai Kumar Gupta, Enketeswara Subudhi
Summary: This study explores enzymes in a carbohydrate-contaminated unique environment at Deulajhari hot spring using a multi-omics approach. The study identifies a diverse range of potential lignocellulose-digesting enzymes and highlights their significant potential in industrial-scale biofuel production.
RENEWABLE & SUSTAINABLE ENERGY REVIEWS
(2023)
Review
Green & Sustainable Science & Technology
Wangliang Li, Rohit Gupta, Zhikai Zhang, Lixia Cao, Yanqing Li, Pau Loke Show, Sunil Kumar, Vijaya Kumar Gupta, Kun-Yi Andrew Lin, Sunita Varjani, Stephanie Connelly, Siming You
Summary: High-solid anaerobic digestion (HSAD) is an effective method for disposing of organic waste and mitigating climate change through bioenergy recovery. However, HSAD faces challenges such as low biogas and methane yields, slow reaction rates, and inhibition due to mixing limitations. This article critically reviews recent progress in HSAD, focusing on transport phenomena and process modeling. It discusses hydrodynamic phenomena, biokinetic mechanisms, reactor simulations, advanced reactor designs, industrial applications, and key parameters for sustained HSAD operation. Further research on novel materials and the development of a generic simulation tool are needed for improving HSAD efficiency and scalability.
RENEWABLE & SUSTAINABLE ENERGY REVIEWS
(2023)
Article
Energy & Fuels
Zeba Usmani, Minaxi Sharma, Manikant Tripathi, Abdul Sattar Nizami, Liang Gong, Munagala S. Reddy, Vijay Kumar Thakur, Vijai Kumar Gupta, Quang D. Nguyen
Summary: With concerns over fossil fuels and waste, there is a focus on finding alternate energy sources for environmental protection and sustainable development. Biomass waste has emerged as a new feedstock for renewable energy production, but it requires pretreatment and valorization strategies. Microwave heating is being explored as an alternative to conventional heating for its advantages. This review explores the application of microwaves as a pretreatment technique and addresses challenges and future research directions.
JOURNAL OF THE ENERGY INSTITUTE
(2023)
