期刊
WASTE MANAGEMENT
卷 34, 期 3, 页码 642-652出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.wasman.2013.11.010
关键词
Coffee husk compost; Organic matter degradation; Enzyme activity; COMPOCHIP microarray; PCR-DGGE
资金
- Fonds zur Forderung der wissenschaftlichen Forschung (F'WF) Austria [FP200010]
- Fundacion Alfonso Martin Escudero
- Austrian Science Fund (FWF) [P 20001] Funding Source: researchfish
Various parameters were measured during a 90-day composting process of coffee husk with cow dung (Pile 1), with fruit/vegetable wastes (Pile 2) and coffee husk alone (Pile 3). Samples were collected on days 0, 32 and 90 for chemical and microbiological analyses. C/N ratios of Piles 1 and 2 decreased significantly over the 90 days. The highest bacterial counts at the start of the process and highest actinobacterial counts at the end of the process (Piles 1 and 2) indicated microbial succession with concomitant production of compost relevant enzymes. Denaturing gradient gel electrophoresis of rDNA and COMPOCHIP microarray analysis indicated distinctive community shifts during the composting process, with day 0 samples clustering separately from the 32 and 90-day samples. This study, using a multi-parameter approach, has revealed differences in quality and species diversity of the three.composts. (C) 2013 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license
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