Article
Chemistry, Analytical
Liguo Song, Gabrielle Valenzuela, Shelby Carlson, Zachary Dodson, Mojisola Adisa
Summary: The growing demand for accurate cannabinoid quantification has led to the development of a liquid chromatography diode array detector (LC-DAD) method, which significantly improved existing methods by targeting 20 cannabinoids and quantifying 18 of them.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Fuyu Guan, Youwen You, Savannah Fay, Xiaoqing Li, Mary A. Robinson
Summary: A novel untargeted drug detection method (UDD) has been developed to address the limitations of current targeted analytical methods in detecting doping agents. By processing and analyzing liquid chromatography-high resolution mass spectrometry data, the majority of 57 drugs were accurately detected using a mathematical model and two algorithms. The study represents a significant advancement in using artificial intelligence for the detection of both known and emerging doping agents in sports.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Zhanxuan E. Wu, Marlena C. Kruger, Garth J. S. Cooper, Ivana R. Sequeira, Anne-Thea McGill, Sally D. Poppitt, Karl Fraser
Summary: Untargeted metabolomics of blood samples has been widely used to study metabolic alterations in diseases and identify biomarkers. This study found that the concentration of metabolites in plasma was more reflective of the liver profile than muscle or adipose tissue, highlighting the importance of considering the metabolomic relationship between different tissues and plasma.
Article
Plant Sciences
Alison Green, Guillermo Federico Padilla-Gonzalez, Methee Phumthum, Monique S. J. Simmonds, Nicholas J. Sadgrove
Summary: Commercially used for treating chronic venous insufficiency, A. hippocastanum L. fruits contain beta-aescin which exhibits anti-inflammatory effects. However, diverse organ-specific secondary metabolites have been overlooked as potential pharmacological opportunities. Untargeted metabolomics analysis revealed unique chemical profiles in different organs of Aesculus plants, with fruits showing the highest antifungal and antioxidant activities. This suggests potential therapeutic leads for further exploration in the entire genus of Aesculus.
Article
Food Science & Technology
Frank Klont, Stepan Stepanovic, Daan Kremer, Ron Bonner, Daan J. Touw, Eelko Hak, Stephan J. L. Bakker, Gerard Hopfgartner
Summary: This study describes improved strategies for detecting chemical exposures and their application in a non-targeted metabolomics study on kidney transplant recipients. The new methods can detect more chronic exposures and improve the reliability of detecting intermittent exposures.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Environmental Sciences
Kelly Poitou, Tiphaine Rogez-Florent, Marie Lecoeur, Cecile Danel, Romain Regnault, Philippe Verite, Christelle Monteil, Catherine Foulon
Summary: This study developed two methods for screening plasticizers in gloves and compared their performance and results. It found that the main plasticizers in gloves were DEHP, DEHT, and DINP, with varying contents across different glove materials, highlighting a predominant use of plasticizers in vinyl gloves.
Article
Psychiatry
Meng Song, Ya Liu, Jiahui Zhou, Han Shi, Xi Su, Minglong Shao, Yongfeng Yang, Xiujuan Wang, Jingyuan Zhao, Dong Guo, Qing Liu, Luwen Zhang, Yan Zhang, Luxian Lv, Wenqiang Li
Summary: Using liquid chromatography mass spectrometry, the study identified potential biomarkers associated with the diagnosis and treatment of schizophrenia. Biomarker panels were selected to distinguish between schizophrenia and healthy controls, as well as between schizophrenia before and after medication. Disturbances in lipid metabolism, sulfation modification, tryptophan metabolism, anti-inflammatory and antioxidant systems, and unsaturated fatty acids metabolism were observed in schizophrenia. These findings could contribute to the development of objective diagnostic and drug treatment monitoring tools for schizophrenia.
PSYCHIATRY RESEARCH
(2023)
Article
Immunology
Meilin Ding, Zha Zhen, Mei Ju, Suolang Quzong, Xuesi Zeng, Xiaoxia Guo, Rui Li, Mingming Xu, Jingjing Xu, Hongyang Li, Wei Zhang
Summary: Our study found significant differences in the metabolomic profiles between vitiligo patients and healthy subjects in both plateau and plain areas, with significantly higher levels of S1P in the serum of vitiligo patients and healthy subjects in the plateau area. These findings suggest that alterations in S1P metabolism may be involved in the pathogenesis of vitiligo.
CLINICAL IMMUNOLOGY
(2023)
Review
Chemistry, Analytical
Hanne Roberg-Larsen, Elsa Lundanes, Tuula A. Nyman, Frode S. Berven, Steven Ray Wilson
Summary: Single-cell analysis provides in-depth insights into diseases and diagnostics, with liquid chromatography playing a crucial role. The development of novel sample preparation techniques and improvements in mass spectrometry are enhancing the promise of single-cell analysis. However, technical challenges and the need for higher throughput and robustness may lead to the reinvention of alternative nano LC column formats.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Yasin El Abiead, Christoph Bueschl, Lisa Panzenboeck, Mingxun Wang, Maria Doppler, Bernhard Seidl, Juergen Zanghellini, Pieter C. Dorrestein, Gunda Koellensperger
Summary: This study utilized 13C labeled and unlabeled Pichia pastoris extracts to identify heterogeneous multimerization in biological samples and successfully annotated the monomeric partners of these heteromers. Additionally, they created the first MS/MS library that included data from heteromultimers and demonstrated the relevance of these newly annotated ions to other publicly available datasets. Furthermore, their workflow detected metabolite features originating from heterodimers in other datasets as well.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Eva-Maria Harrieder, Fleming Kretschmer, Sebastian Boecker, Michael Witting
Summary: Metabolomics and lipidomics involve the large-scale analysis of metabolites, requiring advanced analytical methods. Liquid Chromatography-Mass Spectrometry (LC-MS) is a commonly used technique that provides different selectivities in separation and high sensitivity in detection. Due to the huge chemical diversity, there is no single analysis method that can cover the entire range of metabolites or lipids, leading to the use of different separation methods. This review explores the current use of LC-MS in metabolomics and lipidomics using data from public databases, and highlights potential future trends and improvements needed.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Minnie Jacob, Refat M. Nimer, Mohamad S. Alabdaljabar, Essa M. Sabi, Mysoon M. Al-Ansari, Maged Housien, Khalid M. Sumaily, Lina A. Dahabiyeh, Anas M. Abdel Rahman
Summary: This study used liquid chromatography-mass spectrometry (LC-MS) metabolomics to analyze the metabolome of serum from patients with nephrotic syndrome (NS) and found significant changes in 176 metabolites compared to the control group. Dysregulation of arginine, proline, and tryptophan metabolism, as well as arginine, phenylalanine, tyrosine, and tryptophan biosynthesis, were the most common metabolic pathways affected in NS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Yanru Zhu, Feng Wang, Jiatong Han, Yunli Zhao, Miao Yu, Mingyan Ma, Zhiguo Yu
Summary: This study investigated the preventive effect and mechanism of L-theanine on depression in juvenile rats. The results confirmed the preventive effect of L-theanine and identified potential biomarkers related to its treatment. Overall, L-theanine achieved significant preventive results on depression by regulating various aspects of the body, such as amino acids, lipids, and inflammation.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2023)
Article
Food Science & Technology
Dhara Dixit
Summary: This study reveals the biochemical traits, mineral makeup, and secondary metabolite outline of Iyengaria stellata using untargeted metabolite profiling. A total of 108 putative metabolites were detected, including steroids, terpenoids, ketones, esters, polyphenols, anthraquinones, tocopherol, etc. The study highlights the potential of I. stellata as a natural source in pharmaceutical and nutraceutical compositions.
Article
Endocrinology & Metabolism
Jun Kou, Chunyang He, Lin Cui, Zhengping Zhang, Wei Wang, Li Tan, Da Liu, Wei Zheng, Wei Gu, Ning Xia
Summary: The study on Chinese postmenopausal women with osteoporosis identified potential biomarkers related to metabolic pathway disorders, providing new insights for early diagnosis. The findings suggest that metabonomic analysis has great potential for application in postmenopausal women with osteoporosis.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Chemistry, Medicinal
Danielle Copmans, Sara Kildgaard, Emma Roux, Michele Partoens, Gert Steurs, Xinhui Wang, Wim M. De Borggraeve, Camila Esguerra, Alexander D. Crawford, Thomas O. Larsen, Peter A. M. de Witte
Summary: This study reports the discovery of two new antiseizure compounds, 2,5-diketopiperazine halimide and plinabulin, both known microtubule destabilizing agents. Plinabulin has the potential for drug repurposing as it is already in clinical trials for other diseases. Both compounds showed antiseizure activity in animal models, particularly against drug-resistant seizures.
Article
Biotechnology & Applied Microbiology
Johan Vormsborg Christiansen, Thomas Isbrandt, Rasmus Asferg, Scott A. Jarmusch, Thomas Ostenfeld Larsen, Jens Christian Frisvad
Summary: This study aimed to explore the production of fungal quinones in wild-type strains for use as electrolytes in redox flow batteries. As society converts energy production in a more sustainable direction, it becomes increasingly more important to store sustainable energy in smart ways. Conventional battery technologies imply the use of highly toxic, expensive, and rare metals; thus, quinone redox flow batteries have been proposed to be a desirable alternative. In this study, we explored the possibility of producing the fungal quinone phoenicin in Penicillium spp. by changing the growth parameters. The production of other secondary metabolites and known mycotoxins was also investigated in a metabolomics study. It was shown that phoenicin production was activated by optimizing the carbon concentration of the medium, resulting in high titers and purity of the single metabolite.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Mycology
Maria Luisa Maldonado, Andrea Patriarca, Patricia Mc Cargo, Leopoldo Iannone, Vicente Sanchis, Kristian Fog Nielsen, Virginia Fernandez Pinto
Summary: The study compared two populations of P. expansum from Argentina and Spain, finding no significant differences in morphology, secondary metabolite profiles, patulin accumulation, and phylogenetic analysis. Greater diversity of P. expansum was observed in Spain compared to Argentina, possibly due to haplotype exchange between the two countries and subsequent adaptation to environmental conditions or apple varieties grown in each region.
Article
Biochemistry & Molecular Biology
Johan Vormsborg Christiansen, Thomas Ostenfeld Larsen, Jens Christian Frisvad
Summary: Fungal quinones have diverse applications, but the feasibility of a production system must be considered, including quinone yield, purity, extraction ease, and mycotoxin co-production. This study screened filamentous fungi for quinone production and evaluated their potential for optimization.
Article
Biochemistry & Molecular Biology
Julie B. Nielsen, Tetiana Gren, Omkar S. Mohite, Tue S. Jorgensen, Andreas K. Klitgaard, Anna-Sophie Mourched, Kai Blin, Daniel Oves-Costales, Olga Genilloud, Thomas O. Larsen, David Tanner, Tilmann Weber, Charlotte H. Gotfredsen, Pep Charusanti
Summary: Actinomycetes produce a wealth of complex natural products. Researchers have identified the biosynthetic gene cluster for pyracrimycin A and proposed a complete biosynthesis pathway based on feeding experiments and isotope labeling analysis.
ACS CHEMICAL BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Sheng-Da Zhang, Laura Louise Lindqvist, Thomas Isbrandt, Ingrid Lykke Borre, Mario Wibowo, Maike Wennekers Nielsen, Ling Ding, Thomas Ostenfeld Larsen, Lone Gram
Summary: The biosynthetic genetic basis and pathway of solonamides in P. galatheae S2753 were revealed, which may lead to the industrial production of solonamides. The comparison between a solonamide-deficient mutant and wild-type S2753 demonstrated that solonamides stimulate the swimming behavior of S2753 and influence a few key physiological processes of the native producers. These compounds not only serve as novel drug candidates but also provide competitive benefits to the native producer for survival in nature.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Food Science & Technology
Maria Agustina Pavicich, Kristian Fog Nielsen, Andrea Patriarca
Summary: This study characterized the morphology and chemistry of Alternaria isolates from apples, finding low morphological diversity and high chemical diversity. The isolates from conventional apples showed a wider range of metabolites, with a prevalence of altertoxins and alternariols. Isolates from mouldy core had higher ability to produce metabolites from different chemical families than those from external lesions.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Ecology
Morten Lindqvist Hansen, Mario Wibowo, Scott Alexander Jarmusch, Thomas Ostenfeld Larsen, Lars Jelsbak
Summary: Soil and rhizosphere microbiomes play important roles in suppressing plant pathogens through the production of antagonistic secondary metabolites. This study investigates how interactions within the Pseudomonas genus affect their production of antimicrobial metabolites. The researchers found that P. capeferrum species can modulate secondary metabolite production in P. protegens through two distinct mechanisms. They also discovered that the interaction between the two species leads to changes in specific metabolite production. These findings have implications for improving the efficacy of biocontrol strains and consortia.
Review
Biochemistry & Molecular Biology
Xinhui Wang, Scott A. Jarmusch, Jens C. Frisvad, Thomas O. Larsen
Summary: This review covers all known biosynthetic compound families and their structural diversity from black aspergilli, with a focus on metabolites that have been firmly linked to their corresponding BGCs.
NATURAL PRODUCT REPORTS
(2023)
Article
Microbiology
Josue J. Silva, Maria H. P. Fungaro, Xinhui Wang, Thomas O. Larsen, Jens C. Frisvad, Marta H. Taniwaki, Beatriz T. Iamanaka
Summary: This study aimed to investigate the diversity of Aspergillus section Flavi in Brazilian foodstuffs and identified two new species, Aspergillus saccharicola sp. nov. and Aspergillus annui sp. nov. The findings highlight the importance of recognizing the biodiversity of this group in food for reducing health risks.
Article
Chemistry, Multidisciplinary
Charlotte O. O. Wilhelmsen, Alexandros Pasadakis-Kavounis, Johan V. V. Christiansen, Thomas Isbrandt, Mads R. R. Almind, Thomas O. O. Larsen, Johan Hjelm, Jens Laurids Sorensen, Jens Muff
Summary: This study demonstrates the influence of phoenicin and its dimer on the capacity performance of phoenicin as a negolyte in a redox flow battery. The biosynthesized fungal quinone mix of phoenicin shows the ability to store four electrons per phoenicin molecule in a redox flow battery negolyte. A detailed analysis is conducted to understand the chemical stability of phoenicin in an alkaline environment.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Environmental Sciences
Jianbo Jian, Zhangyan Wu, Arisbe Silva-Nunez, Xiaohui Li, Xiaomin Zheng, Bei Luo, Yun Liu, Xiaodong Fang, Christopher T. Workman, Thomas Ostenfeld Larsen, Per Juel Hansen, Eva C. Sonnenschein
Summary: This study successfully assembled the genomes of two Prymnesium parvum strains using PacBio HiFi long reads and DNBseq short reads, providing valuable resources for studying harmful algal blooms.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Chemistry, Multidisciplinary
Peter Klemmed Bjork, Nicolai Tidemand Johansen, Nanna Weise Havshoi, Silas Anselm Rasmussen, Johan orskov Ipsen, Thomas Isbrandt, Thomas Ostenfeld Larsen, Anja Thoe Fuglsang
Summary: The specific components trichorzin PA (tPA) II and tPA VI in Trichoderma spp. fungal extracts can enhance the activity of H+-ATPase on plant membranes, thereby promoting plant growth. These components belong to the class of peptaibols, which can form indiscriminate ion channels and exhibit pesticidal activity. Peptaibol-mediated H+-ATPase activation is a concentration-dependent balance.
Article
Electrochemistry
Charlotte Overgaard Wilhelmsen, Sebastian Birkedal Kristensen, Oliver Nolte, Ivan A. Volodin, Johan Vormsborg Christiansen, Thomas Isbrandt, Trine Sorensen, Celine Petersen, Teis Esben Sondergaard, Kare Lehmann Nielsen, Thomas Ostenfeld Larsen, Jens Christian Frisvad, Martin D. Hager, Ulrich S. Schubert, Jens Muff, Jens Laurids Sorensen
Summary: Aqueous organic redox flow batteries (AORFBs) are gaining interest as a potential solution for sustainable energy storage. In this study, a new electrolyte based on the fungal compound phoenicin was proposed and produced through biosynthesis, offering scalability. It was demonstrated that biologically produced phoenicin as a negative electrolyte showed promising performance in an RFB, and the stability of the active material was investigated.
BATTERIES & SUPERCAPS
(2022)
Meeting Abstract
Clinical Neurology
D. Copmans, S. Kildgaard, E. Roux, M. Partoens, G. Steurs, X. Wang, W. M. De Borggraeve, C. V. Esguerra, A. D. Crawford, T. O. Larsen, P. A. M. de Witte