Review
Biochemistry & Molecular Biology
Sajid Iqbal, Farida Begum, Ali A. A. Rabaan, Mohammed Aljeldah, Basim R. Al R. Shammari, Abdulsalam Alawfi, Amer Alshengeti, Tarek Sulaiman, Alam Khan
Summary: Despite being overlooked, Bacillus subtilis has attracted commercial interest as a therapeutic agent, food preservative, and plant-pathogen control agent due to its ability to withstand harsh conditions and synthesize various metabolites. However, the accessibility and fermentation yields of these metabolites are limited. In this review, we comprehensively visualize and classify the antimicrobial peptides and metabolites produced by B. subtilis based on their biosynthetic pathways and chemical structures, providing detailed discussions on their biosynthetic pathways, bioactivity, and chemical structure. This review aims to reignite interest in the often disregarded B. subtilis and its remarkable biosynthetic capabilities.
Review
Plant Sciences
Yi-Ming Chiang, Clay C. C. Wang, Tzu-Shyang Lin
Summary: Fungal natural products have a wide range of bioactivities, and the analysis of fungal genomes has shown that there is great potential for the production of undiscovered natural products. To tap into this resource, the use of genetically tractable fungi as hosts for total heterologous biosynthesis has become increasingly popular in recent years. This review summarizes the methods used to accomplish total heterologous biosynthesis of fungal natural products using Aspergillus nidulans as the host.
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Chemistry, Multidisciplinary
Wei Zhang, Xuan Zhang, Dandan Feng, Yajing Liang, Zhenying Wu, Siyu Du, Yu Zhou, Ce Geng, Ping Men, Chunxiang Fu, Xuenian Huang, Xuefeng Lu
Summary: This study discovered a biosynthetic pathway for a fungal flavonoid and revealed a novel flavonoid biosynthetic pathway. A core nonribosomal peptide synthetase-polyketide synthase (NRPS-PKS) was found responsible for the synthesis of the key precursor chalcone, which was then converted into flavanone by a new type of chalcone isomerase. Finally, a new type of flavone synthase catalyzed the desaturation of flavanone to flavone.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Microbiology
Mary L. Shenouda, Maria Ambilika, Elizabeth Skellam, Russell J. Cox
Summary: Trichoderma reesei has been developed as a microbial cell factory for the heterologous expression of fungal secondary metabolites. By inactivating sorbicillinoid biosynthesis and constructing vectors for the rapid cloning and expression of heterologous fungal biosynthetic genes, this strain can produce desired natural products on waste materials. This new method provides a way to convert waste biomass into secondary metabolites.
Review
Microbiology
Nicolas Pedrini
Summary: Entomopathogenic fungi, such as Beauveria bassiana, produce toxic secondary metabolites that play a role in fungal invasion and immune suppression. The precise function of these metabolites is not well understood but they are typically associated with virulence. This review aims to summarize the available information on the expression of B. bassiana NRPS and PKS genes in different insects during infection, and their role as virulence factors, antimicrobials, or immunosuppressants in the context of fungus-insect interactions.
Article
Microbiology
Rafael Wesley Bastos, Daniel Akiyama, Thaila Fernanda dos Reis, Ana Cristina Colabardini, Rafael Sanchez Luperini, Patricia Alves de Castro, Regina Lucia Baldini, Taicia Fill, Gustavo H. Goldman
Summary: This study annotated the secondary metabolites produced during coculture of Pseudomonas aeruginosa and Aspergillus fumigatus in biofilm formation under both normoxia and hypoxia conditions. It was found that Pseudomonas aeruginosa produces various compounds with inhibitory and antibiofilm effects against the fungus, while little is known about the fungal compounds produced in counterattack.
Article
Microbiology
Ambika Pokhrel, Jeffrey J. Coleman
Summary: The Fusarium solani species complex (FSSC) is a complex of at least 77 phylogenetically distinct species that includes agriculturally important and clinically relevant opportunistic pathogens. The FSSC has been found to produce a diverse range of secondary metabolites, which may contribute to its ability to colonize different habitats and hosts. This study identified a large number of secondary metabolite biosynthetic gene clusters (BGCs) in the genomes of ten FSSC fungi, including several novel BGCs and those responsible for the production of known compounds.
Article
Chemistry, Organic
Yaojie Guo, Fabiano J. Contesini, Xinhui Wang, Simone Ghidinelli, Ditte S. Tornby, Thomas E. Andersen, Uffe H. Mortensen, Thomas O. Larsen
Summary: A new polyketide-nonribosomal peptide hybrid, calipyridone A, was isolated from the fungal Aspergillus californicus. The biosynthetic gene cluster cpd was identified and confirmed to play a role in the production of calipyridone A. Chemical analyses of mutant strains revealed the biosynthetic pathway of calipyridone A, which showed modest inhibition effects on the plaque-forming ability of SARS-CoV-2.
Review
Biotechnology & Applied Microbiology
Mihaela Bejenari, Teis Esben Sondergaard, Jens Laurids Sorensen
Summary: 6-methylsalicylic acid (6-MSA) is a small polyketide produced by fungi through horizontal gene transfer, serving as a metabolic hub for the production of various complex compounds. The most significant metabolite derived from 6-MSA is the mycotoxin patulin. Other important products include terreic acid and yanuthones. The aculin biosynthetic pathway demonstrates the most advanced modification of 6-MSA, involving non-ribosomal peptide synthase and terpene cyclase. This review summarizes the pathways originating from 6-MSA, gene clusters involved, and resulting biosynthetic pathways.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Jiaqi Liu, Guangzhi Dai, Youming Zhang, Xiaoying Bian
Summary: This review article discusses the latest advances in synthetic biology tools and strategies for optimizing secondary metabolite production in non-model microorganisms. The importance of developing broader non-model microbial chassis as programmable heterologous hosts is highlighted, along with the successful applications of heterologous production of complex secondary metabolites. The perspective of developing ideal cell factories in the artificial intelligence-driven genome era is also emphasized.
BIOTECHNOLOGY ADVANCES
(2022)
Review
Biochemistry & Molecular Biology
Tong Wu, Sandra M. Kerbler, Alisdair R. Fernie, Youjun Zhang
Summary: Synthetic biology in plants has attracted increasing attention, and plant cells may be a promising alternative for the production of high-value metabolites. Commercial plant suspension cell cultures have been successfully used to produce valuable metabolites in a safe and cost-effective manner, but the variety of metabolites biosynthesized using plant platforms is currently limited.
PLANT COMMUNICATIONS
(2021)
Review
Chemistry, Organic
Jamshid Amiri Moghaddam, Theresa Jautzus, Mohammad Alanjary, Christine Beemelmanns
Summary: Marine bacteria are valuable sources of structurally unique bioactive natural products, with recent studies focusing on novel compounds from various classes and their biosynthetic pathways such as fatty acids, terpenes, PKS, NRPS, and hybrid PKS-NRPS biomolecules.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2021)
Review
Cell Biology
Chun Ge, Sujie Zhang, Huiwen Mu, Shaojun Zheng, Zhaoyi Tan, Xintong Huang, Chen Xu, Jianjun Zou, Yubing Zhu, Dong Feng, Jiye Aa
Summary: Ferroptosis is a newly discovered form of regulatory cell death that is distinct from other types of cell death. It is characterized by iron-dependent lipid peroxidation and oxidative perturbation, and can be inhibited by iron chelators and lipophilic antioxidants. This process is implicated in various biological contexts, including iron homeostasis, lipid metabolism, and glutathione metabolism. Ferroptosis is closely related to physiological and pathological processes such as tumor progression, (neuro)degenerative diseases, and hepatic and renal failure. There is a need for the discovery of novel compounds that can modulate ferroptosis, despite the existence of some experimental reagents and approved clinical drugs with anti- or pro-ferroptotic properties. This review discusses the molecular mechanisms of ferroptotic death and its roles in different pathophysiological contexts, as well as chemical compounds and natural products that can induce or inhibit ferroptosis for the prevention and treatment of various diseases. It emphasizes the promising prospects of natural products in ferroptosis-associated therapy due to their multiple components, multiple biotargets, and slight side effects.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Plant Sciences
Kevin Robe, Esther Izquierdo, Florence Vignols, Hatem Rouached, Christian Dubos
Summary: The secretion of Fe-mobilizing coumarins by plant roots is a key factor enabling plants to grow in Fe-depleted soils. While the main molecular actors involved in the biosynthesis and secretion of coumarins have been identified, the precise regulatory mechanisms that tune their production remain poorly understood.
TRENDS IN PLANT SCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Chen-Yu Chiang, Masao Ohashi, Yi Tang
Summary: This review highlights key examples in the integration of heterologous expression into genome mining and biosynthetic investigations in the last decade. By choosing appropriate heterologous hosts, prioritizing BGCs for structural novelty, and using shunt products from heterologous expression, important insights into the chemical logic of biosynthesis can be revealed. This study demonstrates the usefulness and limitations of heterologous biosynthesis in fungal natural product discovery.
NATURAL PRODUCT REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Stephen K. Dolan, Tobias Bock, Vanessa Hering, Rebecca A. Owens, Gary W. Jones, Wulf Blankenfeldt, Sean Doyle
Review
Biochemical Research Methods
Rohan Lowe, Neil Shirley, Mark Bleackley, Stephen Dolan, Thomas Shafee
PLOS COMPUTATIONAL BIOLOGY
(2017)
Article
Microbiology
Eoin D. Mulvihill, Nicola M. Moloney, Rebecca A. Owens, Stephen K. Dolan, Lauren Russell, Sean Doyle
FRONTIERS IN MICROBIOLOGY
(2017)
Article
Mycology
Sean Doyle, Gary W. Jones, Stephen K. Dolan
Article
Biochemistry & Molecular Biology
Audrey Crousilles, Stephen K. Dolan, Paul Brear, Dimitri Y. Chirgadze, Martin Welch
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Review
Microbiology
Stephen K. Dolan, Andre Wijaya, Stephen M. Geddis, David R. Spring, Rafael Silva-Rocha, Martin Welch
Review
Biochemistry & Molecular Biology
Stephen K. Dolan, Martin Welch
ANNUAL REVIEW OF MICROBIOLOGY, VOL 72
(2018)
Article
Biochemistry & Molecular Biology
Joana Magalhaes, Nina Franko, Giannamaria Annunziato, Martin Welch, Stephen K. Dolan, Agostino Bruno, Andrea Mozzarelli, Stefano Armao, Aigars Jirgensons, Marco Pieroni, Gabriele Costantino, Barbara Campanini
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2018)
Article
Mycology
Louise Mc Namara, Stephen K. Dolan, John M. D. Walsh, John C. Stephens, Travis R. Glare, Kevin Kavanagh, Christine T. Griffin
Article
Biotechnology & Applied Microbiology
Stephen K. Dolan, Greicy Pereira, Rafael Silva-Rocha, Martin Welch
MICROBIAL BIOTECHNOLOGY
(2020)
Article
Microbiology
Stephen K. Dolan, Michael Kohlstedt, Stephen Trigg, Pedro Vallejo Ramirez, Christoph Wittmann, Clemens Kaminski, Martin Welch
Review
Biochemistry & Molecular Biology
Stephen K. Dolan
JOURNAL OF MOLECULAR BIOLOGY
(2020)
Article
Microbiology
Ainsley Beaton, Cedric Lood, Edward Cunningham-Oakes, Alison MacFadyen, Alex J. Mullins, Walid El Bestawy, Joao Botelho, Sylvie Chevalier, Shannon Coleman, Chloe Dalzell, Stephen K. Dolan, Alberto Faccenda, Maarten G. K. Ghequire, Steven Higgins, Alexander Kutschera, Jordan Murray, Martha Redway, Talal Salih, Ana C. da Silva, Brian A. Smith, Nathan Smits, Ryan Thomson, Stuart Woodcock, Martin Welch, Pierre Cornelis, Rob Lavigne, Vera van Noort, Nicholas P. Tucker
FEMS MICROBIOLOGY LETTERS
(2018)
Article
Microbiology
Rajesh Padumane Shastry, Stephen K. Dolan, Yassmin Abdelhamid, Ravishankar Rai Vittal, Martin Welch
FEMS MICROBIOLOGY LETTERS
(2018)
Article
Cell Biology
Adriana Oliveira Manfiolli, Patricia Alves de Castro, Thaila Fernanda dos Reis, Stephen Dolan, Sean Doyle, Gary Jones, Diego M. Riano Pachon, Mevluet Ulas, Luke M. Noble, Derek J. Mattern, Axel A. Brakhage, Vito Valiante, Rafael Silva-Rocha, Ozgur Bayram, Gustavo H. Goldman
CELLULAR MICROBIOLOGY
(2017)