期刊
VISION RESEARCH
卷 93, 期 -, 页码 43-48出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.visres.2013.10.008
关键词
Rhodopsin, GPCR; Photoactivatable GFP; Photoreceptors; Trafficking; paGFP
资金
- National Eye Institute [EY019311, T32 GM008111]
- EyeSight Foundation of Alabama
- Karl Kirchgessner Foundation
- Plum Foundation Research Award
- Hartline-MacNichol Research Award
- E. Matilda Ziegler Foundation
Rhodopsin is trafficked to the rod outer segment of vertebrate rod cells with high fidelity. When rhodopsin transport is disrupted retinal photoreceptors apoptose, resulting in the blinding disease autosomal dominant retinitis pigmentosa. Herein, we introduce rhodopsin-photoactivatable GFP-1D4 (rhodopsin-paGFP-1D4) for the purposes of monitoring rhodopsin transport in living cells. Rhodopsin-paGFP-1D4 contains photoactivatable GFP (paGFP) fused to rhodopsin's C-terminus and the last eight amino acids of rhodopsin (1D4) appended to the C-terminus of paGFP. The fusion protein binds the chromophore 11-cis retinal and photoisomerizes upon light activation similarly to rhodopsin. It activates the G-protein transducin with similar kinetics as does rhodopsin. Rhodopsin-paGFP-1D4 localizes to the same compartments, the primary cilium in cultured IMCD cells and the outer segment of rod cells, as rhodopsin in vitro and in vivo. This enables its use as a model of rhodopsin transport and details the importance of a free rhodopsin C-terminus in rod cell localization and health. (C) 2013 Elsevier Ltd. All rights reserved.
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