Article
Biochemistry & Molecular Biology
Vanesa Madan, Lucas Albacete-Albacete, Li Jin, Pietro Scaturro, Joseph L. Watson, Nadine Muschalik, Farida Begum, Jerome Boulanger, Karl Bauer, Michael A. Kiebler, Emmanuel Derivery, Simon L. Bullock
Summary: HEATR5B is a novel player in dynein-dynactin function, directly binding to the dynein tail and dynactin, and stimulating the motility of AP1-associated endosomal membranes. HEATR5B also promotes association of AP1 with endosomal membranes independently of dynein.
Article
Biology
Juliane Da Graca, Juliette Charles, Morgane Djebar, Karla Alvarez-Valadez, Joelle Botti, Etienne Morel
Summary: Nutrient deprivation, or starvation, is a significant stress that mammalian cells face in various conditions. In this study, the researchers discovered that starvation leads to the connection of endosomal tubules and ER subregions, promoting the formation of autophagosomes. They also found that SNX1 protein is crucial in regulating this endosomal membrane generation and its importance in the autophagic response. The study highlights the contribution of early endosomes in the cellular response to nutritional stress.
LIFE SCIENCE ALLIANCE
(2023)
Article
Biochemistry & Molecular Biology
Zuodong Ye, Dawei Wang, Yingying Lu, Yunjiao He, Jingting Yu, Wenjie Wei, Chang Chen, Rui Wang, Liang Zhang, Liangren Zhang, Minh T. N. Le, William C. Cho, Mengsu Yang, Hongmin Zhang, Jianbo Yue
Summary: V1 shows significant anti-metastatic activity by inhibiting cancer cell migration and metastasis in vitro and in vivo, as well as suppressing colony formation, migration, and invasion of cancer cells. V1 targets CapZ beta to inhibit endosomal trafficking and metastasis processes.
Review
Microbiology
Tran X. N. Huy, Trang T. Nguyen, Heejin Kim, Alisha W. B. Reyes, Suk Kim
Summary: Brucella species is the causative agent of brucellosis, causing significant economic losses and public health threats. Brucella invades host cells and survives through pathogen-host interactions, utilizing various mechanisms to evade immune responses.
Article
Materials Science, Multidisciplinary
Yiming Geng, Shengyun Huang, Li Ma, Mingyang Li, Enli Yang, Yiming Li, Dongsheng Zhang, Xiao Fu, Haiwei Wu
Summary: Autophagy is a cellular process that captures and degrades intracellular components to maintain metabolism and homeostasis. In late stage tumorigenesis, autophagy promotes the survival, growth, and aggressiveness of tumors, as well as drug resistance. Nanoparticles can induce autophagy and offer a new perspective for tumor therapy strategies.
APPLIED MATERIALS TODAY
(2023)
Article
Biochemistry & Molecular Biology
Hao Wang, Guangxu Bai, Jianwei Chen, Wen Han, Ran Guo, Na Cui
Summary: The study found that during sepsis, the mTOR pathway modulates CD4 + T cell autophagy and apoptosis by regulating the transcription of A-L fusion-related proteins. Further experiments demonstrated that deletion of mTOR could alleviate fusion dysfunction between autophagosomes and lysosomes in CD4 + T cells, improving cell apoptosis.
Editorial Material
Cell Biology
Yuchen Lei, Daniel J. Klionsky
Summary: Recent studies have shown that calcium plays a regulatory role in various stages of autophagy, and the release of calcium from lysosomes is crucial for autophagosome and lysosome fusion. MCOLN3/TRPML3, a calcium-permeable channel located on both lysosomes and autophagosomes, has been identified as an autophagy regulator and a potential supplier of calcium for autophagic fusion.
Article
Chemistry, Analytical
Hyunbin Kim, Hae Nim Lee, Jaesik Choi, Jihye Seong
Summary: Internalized B2ARs can sustain their activity and cAMP production for several hours during the endosomal trafficking pathway. The temporal kinetics of B2AR activity can be well explained by an active-vesicle population model modified from the Ricker model.
ANALYTICAL CHEMISTRY
(2021)
Article
Cell Biology
Xueyu Wang, Zhiqiang Wei, Tingyu Lan, Yulin He, Bin Cheng, Ruimin Li, Hongxia Chen, Fahong Li, Guohua Liu, Bin Jiang, Yong Lin, Mengji Lu, Zhongji Meng
Summary: CCDC88A/GIV enhances HBV replication by increasing endosomal trafficking and reducing autophagic degradation of HBV antigens. This suggests that CCDC88A/GIV-mediated endosomal trafficking plays a significant role in HBV replication and progeny secretion.
Article
Biochemistry & Molecular Biology
Tarhan Ibrahim, Virendrasinh Khandare, Federico Gabriel O. Mirkin, Yasin Tumtas, Doryen Bubeck, Tolga Bozkurt
Summary: This article introduces a protein modeling method called AF2-multimer, which accurately predicts AIM/LIR sequences related to autophagy in host cells. By combining evolutionary analysis of protein sequences and protein-protein interaction experiments, the researchers found that AF2-multimer can predict physiologically relevant AIM sequences and noncanonical AIM sequences in ATG3 from potato. In addition, AF2-multimer can predict pathogen-specific AIM sequences targeting ATG8 members and reveals the existence of cross-kingdom AIM/LIR associations. The application of AF2-multimer is expected to greatly accelerate our understanding of the molecular basis of autophagy in all biological kingdoms.
Article
Biology
Pushpanjali Soppina, Nishaben Patel, Dipeshwari J. Shewale, Ashim Rai, Sivaraj Sivaramakrishnan, Pradeep K. Naik, Virupakshi Soppina
Summary: The study reveals a fine balance between ATP hydrolysis rate and microtubule affinity in Kinesin-3 motors, resulting in distinct mechanical outputs.
Article
Multidisciplinary Sciences
Zhen Feng, Cheng-han Yu
Summary: The production of PI(3,4)P2 promotes invasive cell migration by stimulating the trafficking of integrin receptor at the invadopodium.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Physiology
Mirco Glitscher, Eberhard Hildt
Summary: Cholesterol plays a crucial role in the life cycle of viruses and can be targeted as a novel antiviral strategy. Its impact on both viral metabolism and innate immunity highlights the importance of studying cellular cholesterol redistribution in the context of viral infections. Understanding the regulatory roles of the endosomal system could lead to the development of pan-antiviral treatments.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Engineering, Chemical
Rui Shen, Dingding Lue, Guanyu Chen, Mengjin Liu, Shiqi Pu, Yiling Zhang, Qiang Wang, Ping Qian, Xudong Tang
Summary: In this study, it was found that Bombyx mori nucleopolyhedrovirus (BmNPV) enhances its own replication by taking advantage of the host SUMOylation system. The protein kinase-interacting protein (PKIP) that is conserved in all Alphabaculoviruses was found to be modified by SUMO at lysine K70. Mutation of K70 in PKIP resulted in its translocation from the cytoplasm to the nucleus. Rescue experiments showed that restoring wild-type PKIP in the mutant virus restored BmNPV replication, but this was not the case for the K70R mutation.
Review
Biochemistry & Molecular Biology
Maria J. Iraburu, Tommy Garner, Cristina Montiel-Duarte
Summary: The endocytosis of ligand-bound receptors and their recycling to the plasma membrane are important for cell functions. The insulin receptor can be endocytosed by clathrin-dependent and independent mechanisms, and the sorting of the receptor at the early endosome determines the intensity and duration of insulin effects. Various proteins, including the Arf and Rab families of small GTPases, regulate the endocytic and endosomal pathways. This article argues for a specific role of the slow recycling route in INSR endocytosis and provides a review of the molecular mechanisms involved.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Jinxu Fang, Liang Xiao, Kye-Il Joo, Yarong Liu, Chupei Zhang, Shuanglong Liu, Peter S. Conti, Zibo Li, Pin Wang
INTERNATIONAL JOURNAL OF CANCER
(2016)
Review
Chemistry, Multidisciplinary
Yun Kee Jo, Hyo Jeong Kim, Yeonsu Jeong, Kye Il Joo, Hyung Joon Cha
ADVANCED MATERIALS INTERFACES
(2018)
Article
Biochemistry & Molecular Biology
Liang Xiao, Kuo-Chan Hung, Terry T. Takahashi, Kye-Il Joo, Matthew Lim, Richard W. Roberts, Pin Wang
ACS CHEMICAL BIOLOGY
(2013)
Article
Chemistry, Multidisciplinary
Yeonsu Jeong, Yun Kee Jo, Bum Jin Kim, Byeongseon Yang, Kye Il Joo, Hyung Joon Cha
Article
Biochemistry & Molecular Biology
Yarong Liu, Liang Xiao, Kye-Il Joo, Biliang Hu, Jinxu Fang, Pin Wang
Article
Engineering, Biomedical
Kye-Il Joo, Liang Xiao, Shuanglong Liu, Yarong Liu, Chi-Lin Lee, Peter S. Conti, Michael K. Wong, Zibo Li, Pin Wang
Article
Biochemistry & Molecular Biology
Y. Liu, K-I Joo, P. Wang
Article
Engineering, Biomedical
Yarong Liu, Victoria Fox, Yuning Lei, Biliang Hu, Kye-Il Joo, Pin Wang
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2014)
Article
Chemistry, Multidisciplinary
Muxun Zhao, Yarong Liu, Renee S. Hsieh, Nova Wang, Wanyi Tai, Kye-Il Joo, Pin Wang, Zhen Gu, Yi Tang
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2014)
Article
Chemistry, Multidisciplinary
Muxun Zhao, Biliang Hu, Zhen Gu, Kye-Il Joo, Pin Wang, Yi Tang
Article
Multidisciplinary Sciences
Liang Xiao, Kye-Il Joo, Matthew Lim, Pin Wang
Article
Multidisciplinary Sciences
Yarong Liu, April Tai, Kye-Il Joo, Pin Wang
Article
Chemistry, Multidisciplinary
Yarong Liu, Yun Fang, Yu Zhou, Ebrahim Zandi, Chi-Lin Lee, Kye-Il Joo, Pin Wang
Article
Materials Science, Biomaterials
Chi-Lin Lee, Yarong Liu, Jason Dang, Kye-Il Joo, Pin Wang
Article
Virology
Mohamed S. H. Hassan, Ahmed Ali, Motamed Elsayed Mahmoud, Danah Altakrouni, Shahnas M. Najimudeen, Mohamed Faizal Abdul-Careem
Summary: This study compared the efficacy of different vaccination programs against Canadian DMV/1639 IBV and found that the autogenous inactivated DMV/1639 vaccine was more effective in reducing viral loads and boosting virus neutralization titers. Both vaccination programs provided significant protection against pathological effects.
Article
Virology
Wanda Christ, Jonas Klingstrom, Janne Tynell
Summary: The integrated stress response (ISR) is activated in response to SARS-CoV-2 infection, triggering translational arrest and inhibiting stress granule formation. However, the stress-responsive transcription factors ATF4 and CHOP are not induced. Different SARS-CoV-2 variants show variant-specific differences in ISR activation, suggesting potential impacts on pathogenesis and treatment strategies.
Article
Virology
Xiao Han, Hankun Xu, Yifan Weng, Rong Chen, Jidong Xu, Tong Cao, Renjie Sun, Ying Shan, Fang He, Weihuan Fang, Xiaoliang Li
Summary: Classical swine fever virus (CSFV) can inhibit the host innate immunity by decreasing the level of high mobility group box 1 (HMGB1), and CSFV Npro can evade host antiviral immune response through upregulating HMGB1 acetylation.
Article
Virology
Sunjian Lyu, Fulei Xiong, Tianpeng Qi, Weifeng Shen, Qi Guo, Mingming Han, Li Liu, Weishao Bu, Julin Yuan, Bao Lou
Summary: This study identified and characterized a novel temperate A. hydrophila phage, P05B, which has strong lytic ability and stability across different temperatures and pH values.
Article
Virology
Siyuan Liu, Wei Chen, Raphael Nyaruaba, Shunlong Wang, Cihan Yang, Qun Wu, Ying Liu, Puyu Liu, Fei Wang, Jingling Wang, Zhiming Yuan, Dingwei Sun, Han Xia
Summary: Specific, sensitive, and reliable RT-qPCR assays for the detection of OYAV and EBIV have been successfully developed in this study. These assays hold great promise for their potential application in clinical and field samples in the future.
Article
Virology
Rui Wang, Qiang Sun, Jinbo Xiao, Congcong Wang, Xiaoliang Li, Jichen Li, Yang Song, Huanhuan Lu, Ying Liu, Shuangli Zhu, Zhijun Liu, Yong Zhang
Summary: Coxsackievirus A6 (CVA6) has emerged as an important agent causing hand, foot, and mouth disease (HFMD). Mutations in residue 64 of the virus have been found to increase resistance to ribavirin and decrease sensitivity to guanidine hydrochloride. This mutation also leads to increased replication fidelity and reduced pathogenicity. These findings provide a foundation for the development of a safe and effective live attenuated CVA6 vaccine.
Article
Virology
Yao Xiong, Keran Ma, Xiao Zou, Yantao Liang, Kaiyang Zheng, Tiancong Wang, Hong Zhang, Yue Dong, Ziyue Wang, Yundan Liu, Hongbing Shao, Andrew Mcminn, Min Wang
Summary: This study isolated a novel virus, vB_VviC_ZQ26, infecting Vibrio cyclitrophicus from coastal waters near Qingdao, China. It was found that vB_VviC_ZQ26 belongs to a new vibriophage-specific family, Coheviridae, and is mainly found in temperate and tropical epipelagic zones.
Article
Virology
Xing Li, Zhiping Ye, Ewan P. Plant
Summary: By analyzing clinical and cultured samples using the same bioinformatic pipeline, it was found that 5' copyback DVGs are prevalent in human clinical samples but not in cultured samples. Furthermore, there are differences in DVG production and composition between in vivo and in vitro infections.
Article
Virology
Shreya Banerjee, Rakesh Sarkar, Arpita Mukherjee, Suvrotoa Mitra, Animesh Gope, Mamta Chawla-Sarkar
Summary: Rotavirus is a major cause of viral gastroenteritis in infants and continues to be a severe issue in underdeveloped nations. Recent research has shown that a long non-coding RNA called SLC7A11-AS1 is upregulated during rotavirus infection and plays a role in facilitating the virus's propagation by regulating intracellular glutathione and lipid peroxidation.
Article
Virology
Eiichi Hondo, Tetsufumi Katta, Ayato Sato, Naoya Kadofusa, Tomoki Ishibashi, Hiroshi Shimoda, Hirokazu Katoh, Atsuo Iida
Summary: Bat-borne emerging viruses, such as the Ebola virus and Nipah virus, pose a significant threat to public health. In this study, we identified micafungin as a potential antiviral drug against Pteropine orthoreovirus (PRV) and found that it inhibits viral release in human cells through activating the endoplasmic reticulum stress response pathway. This research provides valuable insights into the development of effective treatments for future virus pandemics.
Article
Virology
M. Joubert, N. van den Berg, J. Theron, V. Swart
Summary: This study investigated the host gene expression in asymptomatic avocado nursery trees infected with Avocado sunblotch viroid (ASBVd) using RNA sequencing. The results showed that 631 genes were differentially expressed during infection, with 63% upregulated. ASBVd infection significantly affected plant defence responses, phytohormone networks, gene expression pathways, secondary metabolism, cellular transport, as well as protein modification and degradation. This study provides insights into the molecular interactions underlying avsunviroid-host interactions.
Article
Virology
Wanling Zhang, Haiyan Yang, Zhengyun Liu, Shengyu Wang, Tianyang Chen, Hong Song, Yunbin Xu, Fajin Li, Guo Luo, Huan Wang
Summary: EV71 infection leads to changes in mitochondrial morphology and dynamics in human neuroblastoma cells, resulting in mitochondrial dysfunction that may contribute to nervous system dysfunction.
Article
Virology
Mikhail Oliveira Leastro, Elliot Watanabe Kitajima, Vicente Pallas, Jesus Angel Sanchez- Navarro
Summary: This study reports the construction and validation of an infectious cDNA clone of Citrus leprosis virus C (CiLV-C) based on an agroinfection system. Agro-injected Nicotiana benthamiana plants showed localized lesions similar to the natural infection process. The virus recovered from the infected plant tissue could be mechanically transmitted between plants. The CiLV-C reverse genetic system provides a powerful molecular tool for unraveling the peculiarities of the citrus leprosis pathosystem.
Article
Virology
Manel Essaidi-Laziosi, Francisco J. Perez-Rodriguez, Catia Alvarez, Pascale Sattonnet-Roche, Giulia Torriani, Meriem Bekliz, Kenneth Adea, Matthias Lenk, Tasnim Suliman, Wolfgang Preiser, Marcel A. Mueller, Christian Drosten, Laurent Kaiser, Isabella Eckerle
Summary: This study found that the Omicron variant has different replication characteristics compared to other variants of concern, being able to efficiently produce infectious virus in nasal cells but not lung cells. In addition, the Delta and Omicron variants showed increased infection efficiency in A549 cells. These findings suggest that the variants of concern have better adaptation to humans but do not have an extended host range.
Article
Virology
Martin Faye, Modeste Name Faye, Babacar Ndiaye, Moussa Moise Diagne, Safietou Sankhe, Ndeye Marieme Top, Amadou Diallo, Cheikh Loucoubar, Ndongo Dia, Amadou Alpha Sall, Ousmane Faye
Summary: Genomic surveillance in Senegal since March 2020 has detected the emergence of Omicron BA.4 and BA.5 sublineages in June 2022. Next-generation sequencing and phylogeny analysis of isolates from a cluster of cases in Northern Senegal in July 2022 revealed that the BA.4 cases originated from a XAS recombinant, marking the first reported sequence of this variant from Senegal. Continuous genomic surveillance of positive SARS-CoV-2 samples is crucial.