期刊
VIRUS RESEARCH
卷 190, 期 -, 页码 34-39出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.virusres.2014.06.014
关键词
Wheat dwarf virus; Mastrevirus; RNA silencing suppressor; PTGS
类别
资金
- National Key Basic Research Program of China [2012CB114004]
- National Natural Science Foundation of China [31171820]
Wheat dwarf virus (WDV) is an economically important and widespread single-stranded DNA virus in the genus Mastrevirus, family Geminiviridae. So far, an RNA silencing suppressor in the genus Mastrevirus has not been reported. In this study, the viral replication-associated proteins of WDV, Rep and RepA proteins, were demonstrated to have RNA silencing suppressor activities when expressed in agro-infiltrated leaves of Nicotiana benthamiana line 16c, by their ability to inhibit posttranscriptional gene silencing (PTGS) of green fluorescent protein (GFP). On the basis of the analysis of GFP expression and siRNA accumulation, we concluded that both Rep and RepA proteins suppressed local silencing induced by either single-stranded GFP (ssGFP) or inverted repeat GFP (dsGFP) constructs, although their activity can be characterized as relatively weak in comparison to HC-Pro of tobacco etch virus. (C) 2014 Elsevier B.V. All rights reserved.
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