Article
Genetics & Heredity
Cintia N. Parsza, Diego L. M. Gomez, Jorge A. Simonin, Mariano Nicolas Belaich, Pablo D. Ghiringhelli
Summary: The study demonstrated that the budded virions of Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) can transduce and support the expression of delivered genes in mammalian cells but do not replicate. Therefore, AgMNPV has the potential to be used as an alternative to non-infectious viruses in humans for exploring new approaches in gene therapy and other applications based on the use of mammalian cells.
CURRENT GENE THERAPY
(2021)
Review
Biotechnology & Applied Microbiology
Alexandra Marisa Targovnik, Jorge Alejandro Simonin, Gregorio Juan Mc Callum, Ignacio Smith, Franco Uriel Cuccovia Warlet, Maria Victoria Nugnes, Maria Victoria Miranda, Mariano Nicolas Belaich
Summary: Baculoviruses, as insect pathogens, have been widely used in biotechnological applications with various biological engineering features, including pest control, recombinant protein expression systems, mammalian gene delivery, and antigenic protein display systems. BEVS and BacMam technologies, due to their ease of use and availability as commercial systems, have been widely introduced in academia and the pharmaceutical industry as essential tools for developing biological products.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Yong Qi, Shan-Shan Wang, Lu-Lin Li
Summary: Late gene expression of baculovirus depends on the virus-encoded RNA polymerase. The immediate-early gene ie1 plays essential roles in viral early gene expression, late gene expression, and DNA replication. IE1 can independently activate late gene expression, showing nonspecific transcription start sites.
MICROBIOLOGY SPECTRUM
(2023)
Article
Multidisciplinary Sciences
Jianxiang Qiu, Zumei Liu, Zhixin Fang, Wenjiao Wu
Summary: Coiled coils (CCs) are important protein structural motifs and play crucial roles in protein structure and function. This study identified and analyzed 24 CC motifs in a large DNA virus, Autographa californica multiple nucleopolyhedrovirus (AcMNPV), and found that these motifs are involved in various biological interactions and viral replication processes.
Article
Virology
Jianan Huang, Huanlei Liu, Xiaodong Xu
Summary: The baculovirus expression vector system (BEVS) is widely used for producing recombinant proteins. However, there is a risk of homologous recombination between viral genome and functional baculovirus-derived elements associated with foreign genes, which can lead to defective viruses and decreased recombinant protein yields. Shortening the length of homologous fragments and separating them by point mutations can effectively reduce the unfavorable recombination.
Article
Biotechnology & Applied Microbiology
Mark R. Bruder, Marc G. Aucoin
Summary: We have developed a sensitive assay using CRISPR-Cas9-mediated gene targeting to evaluate the phenotypic impact of disrupting Autographa californica multiple nucleopolyhedrovirus (AcMNPV) genes. By disrupting 13 AcMNPV genes, we evaluated their performance in terms of green fluorescent protein production and progeny virus yield. This assay provides an efficient strategy for scrutinizing AcMNPV gene function and developing an optimized recombinant baculovirus expression vector (rBEV) genome.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Sandra Yazmin Jimenez-Hernandez, Jonatan C. Rangel-Nunez, Jorge E. Ibarra, Ma Cristina Del Rincon-Castro
Summary: This study characterized PlxyMNPV_LBIV-11 at a biological, morphological, and molecular level and found its similarity with AcMNPV. It showed infectivity and potential as a biological control agent, although its virulence was lower than AcMNPV.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Immunology
Paula Molinari, Maria Ines Crespo, Guido N. Molina, Nicolas D. Dho, Fabio Marinho, Belkys Maletto, Claude Leclerc, Sergio C. Oliveira, Oscar Taboga, Ignacio Cebrian, Gabriel Moron
Summary: This study analyzes the intracellular mechanisms and signaling pathways involved in CD8(+) T cell activation by capsid display. The results show that baculoviruses can enter dendritic cells (DCs) and efficiently present antigens through intracellular processing. Additionally, it is found that the production of cytotoxic T cells and IL-12 by DCs is TLR9-dependent and STING-independent.
Article
Biochemistry & Molecular Biology
Malgorzata Klosek, Agnieszka Krawczyk-Lebek, Edyta Kostrzewa-Suslow, Ewelina Szliszka, Joanna Bronikowska, Dagmara Jaworska, Grazyna Pietsz, Zenon P. Czuba
Summary: Inflammation plays a crucial role in immune defense, but chronic inflammation may lead to neurodegenerative diseases, atherosclerosis, inflammatory bowel diseases, or cancer. Flavanones found in citrus fruits possess anti-oxidative and anti-inflammatory properties. This study demonstrates that methyl derivatives of flavanone can inhibit inflammation and modulate the production of inflammatory cytokines.
Article
Virology
Yu Shang, Hengrui Hu, Xi Wang, Hualin Wang, Fei Deng, Manli Wang, Zhihong Hu
Summary: Researchers successfully modified a synthetic baculovirus, AcMNPV-WIV-Syn1, into a novel bacmid, AcBac-Syn, for use as a backbone for baculovirus system. By constructing a vector containing a LacZ:attTn7 and egfp cassette and recombining it with the AcMNPV-WIV-Syn1 genome in yeast, they were able to generate the bacmid AcBac-Syn. The resulting bacmid showed similar biological characteristics to the wild-type virus and could be easily used for foreign gene insertion and expression.
Article
Virology
Maria Victoria Nugnes, Alexandra Marisa Targovnik, Adria Mengual-Marti, Maria Victoria Miranda, Carolina Susana Cerrudo, Salvador Herrero, Mariano Nicolas Belaich
Summary: This study utilized CRISPR/Cas9 technology to pseudogenize the p74 gene of AcMNPV, revealing that the amino terminal sector of P74 is sufficient to restore virus infectivity, providing insight for enhancing viral activity and producing improved biopesticides.
Article
Cell Biology
Lisha Joshi, Ioanna Plastira, Eva Bernhart, Helga Reicher, Zhanat Koshenov, Wolfgang F. Graier, Nemanja Vujic, Dagmar Kratky, Richard Rivera, Jerold Chun, Wolfgang Sattler
Summary: Systemic inflammation induces increased synthesis of LPA(5), which activates microglia and promotes neuroinflammation.
Article
Food Science & Technology
Eun Hae Kim, Youn Young Shim, Hye In Lee, Sanghyun Lee, Martin J. T. Reaney, Mi Ja Chung
Summary: The current study found that astragalin and isoquercitrin from chamchwi had anti-neuroinflammatory effects by inhibiting the MAPK signaling pathway. Astragalin and isoquercitrin reduced the inflammatory response in LPS-induced microglial cells and hippocampus in mice.
Article
Virology
Jared R. Erickson, Robert F. Kalejta, Paul D. Friesen
Summary: Multiplication of the invertebrate DNA baculoviruses activates the host DNA damage response (DDR), which promotes virus DNA replication. ATM, the key kinase in the host DNA damage response, was thought to play a critical role. However, this study found that baculoviruses can activate the host DNA damage response in an ATM-independent manner.
JOURNAL OF VIROLOGY
(2022)
Article
Virology
Jeffrey J. Hodgson, Nicolas Buchon, Gary W. Blissard
Summary: Little is known about the trafficking process of viral envelope proteins in insect cells. In this study, a stable Drosophila cell line was engineered to investigate the host proteins and cellular trafficking pathways involved in the delivery of the baculovirus envelope protein GP64 to the plasma membrane. Through a targeted RNA interference screen, several host genes were identified to be important for the trafficking of GP64 to the cell surface. The findings suggest that recycling plays a role in maintaining GP64 at the cell surface. This study provides insight into the understanding of how insect virus envelope proteins are transported and contributes to the knowledge of virus infection processes.
JOURNAL OF VIROLOGY
(2022)
Article
Virology
Leonardo A. Silva, Brenda R. Camargo, Ana Carolina Araujo, Taylice Leonel Batista, Bergmann M. Ribeiro, Daniel M. P. Ardisson-Araujo
Summary: Baculoviruses are commonly used as expression vectors for heterologous proteins, with fusion of the surface antigen gene of hepatitis B virus to the N-terminal of AcMNPV Polh resulting in more regular and higher expression levels of OBs compared to fusion with the C-terminal. The N-terminal fusion protein also showed higher sensitivity in immunoassays with human antibodies against HBsAg, making it more suitable for quantitative assays.
ARCHIVES OF VIROLOGY
(2022)
Article
Microbiology
Matheus A. Duarte, Fabricio S. Campos, Osvaldo F. Araujo Neto, Leonardo A. Silva, Arthur B. Silva, Thalita C. Aguiar, Raissa N. Santos, Ueric J. B. Souza, Giselly B. Alves, Fernando L. Melo, Daniel M. P. Ardisson-Araujo, Raimundo W. S. Aguiar, Bergmann M. Ribeiro
Summary: This study used high-throughput sequencing to characterize virus sequences in wild-caught Ae.aegypti mosquitoes in Tocantins state, Brazil, discovering new viral strains and contributing to the understanding of virus diversity in mosquitoes.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Brenda R. de Camargo, Leonardo A. da Silva, Athos S. de Oliveira, Bergmann M. Ribeiro
Summary: The study presents an easy pipeline for expressing and purifying SARS-CoV-2 nucleocapsid (N) protein in insect cells, allowing for manufacturing serological tests and basic research on cellular responses to SARS-CoV-2.
JOURNAL OF VIROLOGICAL METHODS
(2022)
Article
Virology
Rodrigo Lima Carneiro, Jessica Pires Farias, Josilene Ramos Pinheiro, Jackson Farias, Andre Carloto Vielmo, Alexander Birbrair, Aline Belmok, Fernando Lucas Melo, Bergmann Morais Ribeiro, Gepoliano Chaves, Paloma Oliveira Vidal, Wilson Barros Luiz, Jaime Henrique Amorim
Summary: This study analyzed a fatal case of co-infection with SARS-CoV-2 and FeLV in a cat, and found that the virus was detected in multiple organs, raising questions about the susceptibility of FeLV-positive cats to SARS-CoV-2 infection and their potential as reservoirs for new variants of the virus.
Article
Virology
Ueric Jose Borges de Souza, Raissa Nunes dos Santos, Fernando Lucas de Melo, Aline Belmok, Jucimaria Dantas Galvao, Tereza Cristina Vieira de Rezende, Franciano Dias Pereira Cardoso, Rogerio Fernandes Carvalho, Monike da Silva Oliveira, Jose Carlos Ribeiro Junior, Evgeni Evgeniev Gabev, Ester Cerdeira Sabino, Clarice Weis Arns, Bergmann Morais Ribeiro, Fernando Rosado Spilki, Fabricio Souza Campos
Summary: This study analyzed the relative prevalence of SARS-CoV-2 lineages in the state of Tocantins, Brazil, and identified the viral dispersal routes of the two most abundant lineages. The results highlighted the importance of trade routes in the transportation of the virus from Southeast to Northern Brazil.
Article
Virology
Cassio Resmin, Ethiane R. Santos, Daniel R. Sosa-Gomez, Bergmann Morais Ribeiro, Daniel M. P. Ardisson-Araujo
Summary: In this study, a novel baculovirus named SpcoNPV-CNPSo-72 was isolated from black armyworm Spodoptera cosmioides in Brazil. The virus showed lethal activity against third-instar S. cosmioides caterpillars and exhibited characteristics similar to other alphabaculoviruses.
Article
Microbiology
Leonardo Assis da Silva, Monique da Rocha Queiroz Lima, Brenda Rabello de Camargo, Dyeferson Kened da Silva Coelho Guimaraes, Anabele Azevedo Lima Barbastefano, Raquel Curtinhas de Lima, Paulo Vieira Damasco, Rivaldo Venancio da Cunha, Luiz Jose de Souza, Elzinandes Leal de Azeredo, Luzia Maria De-Oliveira-Pinto, Tatsuya Nagata, Daniel M. P. Ardisson-Araujo, Flavia Barreto dos Santos, Bergmann Morais Ribeiro
Summary: CHIKV is a globally distributed arbovirus, and its laboratory diagnosis is mainly performed by molecular and serological assays. This research developed an ELISA test based on a multi-epitope recombinant protein for specific detection of anti-CHIKV antibodies, showing high sensitivity and specificity with no cross-reactivity.
Article
Virology
Josilene R. Pinheiro, Esther C. dos Reis, Jessica P. Farias, Mayanna M. C. Fogaca, Patricia de S. da Silva, Itana Vivian R. Santana, Ana Luiza S. Rocha, Paloma O. Vidal, Rafael da C. Simoes, Wilson B. Luiz, Alexander Birbrair, Renato S. de Aguiar, Renan P. de Souza, Vasco A. de C. Azevedo, Gepoliano Chaves, Aline Belmok, Ricardo Duraes-Carvalho, Fernando L. Melo, Bergmann M. Ribeiro, Jaime Henrique Amorim
Summary: This study investigated the epidemiological dynamics of COVID-19 in a Brazilian population of nearly 17,000 patients over a 15-month period. The study found that COVID-19 cases and deaths fluctuated over time, with men and individuals over the age of 60 being the most affected by deaths. Furthermore, individuals between the ages of 30 and 44 were the most affected by COVID-19 cases. The study also revealed that viral loads in the nasopharynx were higher during the early symptomatic period. Additionally, the study discovered a significant increase in deaths during the second half of the study period, as early pandemic SARS-CoV-2 lineages were replaced by the variant of concern (VOC) P.1 (Gamma).
Article
Virology
Ueric Jose Borges de Souza, Raissa Nunes dos Santos, Marta Giovanetti, Luiz Carlos Junior Alcantara, Jucimaria Dantas Galvao, Franciano Dias Pereira Cardoso, Feliph Cassio Sobrinho Brito, Ana Claudia Franco, Paulo Michel Roehe, Bergmann Morais Ribeiro, Fernando Rosado Spilki, Fabricio Souza Campos
Summary: This study investigates the genomic epidemiology of the CHIKV virus in Tocantins state, Brazil, and finds that the virus belongs to the ECSA lineage and is closely related to outbreaks in Northeast and Southeast Brazil. The study identifies several frequent non-synonymous mutations in the virus genome, indicating local transmission in the state. The findings highlight the importance of continued genomic surveillance for predicting new infections and improving public health services.
Article
Virology
Leonardo Assis da Silva, Marcos Fernando Basso, Bergmann Morais Ribeiro
Summary: The cotton boll weevil is a major pest of cotton, causing significant losses in yield and fiber quality. In this study, a new virus, named Anthonomus grandis iflavirus 1 (AgIV-1), was discovered in apparently healthy native adult cotton boll weevils using a metagenomic approach. The complete genome sequence of AgIV-1 was obtained, and it could potentially be used for developing control tools for cotton boll weevils.
ARCHIVES OF VIROLOGY
(2023)
Article
Microbiology
Luisa Dan Favilla, Tatiana Sobianski Herman, Camila da Silva Goersch, Rosangela Vieira de Andrade, Maria Sueli Soares Felipe, Anamelia Lorenzetti Bocca, Larissa Fernandes
Summary: This study successfully generated a novel mutant of the dematiaceous fungal genus Fonsecaea by gene deletion and homologous recombination. The mutant showed differences in nutrient supply, viability, and growth compared to the wild-type and reconstituted strains. These findings contribute to a better understanding of the biology and pathogenicity of chromoblastomycosis causative agents.
Correction
Virology
Monique M. van Oers, Elisabeth A. Herniou, Johannes A. Jehle, Peter J. Krell, Adly M. M. Abd-Alla, Bergmann M. Ribeiro, David A. Theilmann, Zhihong Hu, Robert L. Harrison
ARCHIVES OF VIROLOGY
(2023)
Article
Nanoscience & Nanotechnology
Marcela Guimaraes Landim, Marcella Lemos Brettas Carneiro, Graziella Anselmo Joanitti, Carla Tatiana Mota Anflor, David Dobkowski Marinho, Jose Filipe Bacalhau Rodrigues, Wladymyr Jefferson Bacalhau de Sousa, Daniel de Oliveira Fernandes, Beatriz Ferreira Souza, Alicia Simalie Ombredane, Jessica Catarine Frutuoso do Nascimento, Gisela de Jesus Felice, Aline Midori Adati Kubota, Juliana Simas Coutinho Barbosa, John Hideki Ohno, Solomon Kweku Sagoe Amoah, Lindomar Jose Pena, Glecia Virgolino da Silva Luz, Laise Rodrigues de Andrade, Willie Oliveira Pinheiro, Bergmann Morais Ribeiro, Fabio Rocha Formiga, Marcus Vinicius Lia Fook, Mario Fabricio Fleury Rosa, Henry Maia Peixoto, Rodrigo Luiz Carregaro, Suelia de Siqueira Rodrigues Fleury Rosa
Summary: The novel N95 respirator using chitosan nanoparticles has demonstrated enhanced mechanical, antimicrobial, and antiviral characteristics when compared to traditional N95 respirators. Tests have shown increased resistance to aerosol penetration, improved filtration of microbial and viral particles, and strong antimicrobial activity.
Article
Parasitology
Miguel Souza Andrade, Fabricio Souza Campos, Cirilo Henrique de Oliveira, Ramon Silva Oliveira, Aline Alves Scarpellini Campos, Marco Antonio Barreto de Almeida, Vagner de Souza Fonseca, Danilo Simonini-Teixeira, Anaia da Paixao Seva, Andrea Oliveira Dias Temponi, Fernando Maria Magalhaes, Danielle Costa Capistrano Chaves, Maira Alves Pereira, Ludmila Oliveira Lamounier, Givaldo Gomes de Menezes, Sandy Micaele Aquino-Teixeira, Maria Eduarda Goncalves-dos-Santos, Sofia Bernal-Valle, Nicolas Felipe Drumm Muller, Jader da Cruz Cardoso, Edmilson dos Santos, Maria Angelica Mares-Guia, George Rego Albuquerque, Alessandro Pecego Martins Romano, Ana Claudia Franco, Bergmann Morais Ribeiro, Paulo Michel Roehe, Filipe Vieira Santos de Abreu
Summary: This study reports a surveillance effort that confirmed the presence of YFV in NHPs in the state of Minas Gerais (MG), Brazil. The surveillance network, using smartphone applications and coordinated actions of research institutions and health services, successfully monitored and investigated NHP epizootics. YFV infection was confirmed through RT-qPCR and genome sequencing, facilitating vaccination campaigns and highlighting the importance of coordinated surveillance to prevent YFV spillover to humans.
MEMORIAS DO INSTITUTO OSWALDO CRUZ
(2022)
Article
Infectious Diseases
Deborah Anjos, Fabiola Souza Fiaccadori, Carolina do Prado Servian, Simone Goncalves da Fonseca, Adriana Oliveira Guilarde, Moara Alves Santa Barbara Borges, Fernanda Craveiro Franco, Bergmann Morais Ribeiro, Menira Souza
Summary: This study evaluated the positivity rate and viral load of SARS-CoV-2 in clinical samples from 35 COVID-19 patients. The results showed that SARS-CoV-2 RNA can be detected in stool, sera, and urine specimens. Patients with more comorbidities and severe conditions had higher viral loads.
JOURNAL OF CLINICAL VIROLOGY PLUS
(2022)
Article
Virology
Mohamed S. H. Hassan, Ahmed Ali, Motamed Elsayed Mahmoud, Danah Altakrouni, Shahnas M. Najimudeen, Mohamed Faizal Abdul-Careem
Summary: This study compared the efficacy of different vaccination programs against Canadian DMV/1639 IBV and found that the autogenous inactivated DMV/1639 vaccine was more effective in reducing viral loads and boosting virus neutralization titers. Both vaccination programs provided significant protection against pathological effects.
Article
Virology
Wanda Christ, Jonas Klingstrom, Janne Tynell
Summary: The integrated stress response (ISR) is activated in response to SARS-CoV-2 infection, triggering translational arrest and inhibiting stress granule formation. However, the stress-responsive transcription factors ATF4 and CHOP are not induced. Different SARS-CoV-2 variants show variant-specific differences in ISR activation, suggesting potential impacts on pathogenesis and treatment strategies.
Article
Virology
Xiao Han, Hankun Xu, Yifan Weng, Rong Chen, Jidong Xu, Tong Cao, Renjie Sun, Ying Shan, Fang He, Weihuan Fang, Xiaoliang Li
Summary: Classical swine fever virus (CSFV) can inhibit the host innate immunity by decreasing the level of high mobility group box 1 (HMGB1), and CSFV Npro can evade host antiviral immune response through upregulating HMGB1 acetylation.
Article
Virology
Sunjian Lyu, Fulei Xiong, Tianpeng Qi, Weifeng Shen, Qi Guo, Mingming Han, Li Liu, Weishao Bu, Julin Yuan, Bao Lou
Summary: This study identified and characterized a novel temperate A. hydrophila phage, P05B, which has strong lytic ability and stability across different temperatures and pH values.
Article
Virology
Siyuan Liu, Wei Chen, Raphael Nyaruaba, Shunlong Wang, Cihan Yang, Qun Wu, Ying Liu, Puyu Liu, Fei Wang, Jingling Wang, Zhiming Yuan, Dingwei Sun, Han Xia
Summary: Specific, sensitive, and reliable RT-qPCR assays for the detection of OYAV and EBIV have been successfully developed in this study. These assays hold great promise for their potential application in clinical and field samples in the future.
Article
Virology
Rui Wang, Qiang Sun, Jinbo Xiao, Congcong Wang, Xiaoliang Li, Jichen Li, Yang Song, Huanhuan Lu, Ying Liu, Shuangli Zhu, Zhijun Liu, Yong Zhang
Summary: Coxsackievirus A6 (CVA6) has emerged as an important agent causing hand, foot, and mouth disease (HFMD). Mutations in residue 64 of the virus have been found to increase resistance to ribavirin and decrease sensitivity to guanidine hydrochloride. This mutation also leads to increased replication fidelity and reduced pathogenicity. These findings provide a foundation for the development of a safe and effective live attenuated CVA6 vaccine.
Article
Virology
Yao Xiong, Keran Ma, Xiao Zou, Yantao Liang, Kaiyang Zheng, Tiancong Wang, Hong Zhang, Yue Dong, Ziyue Wang, Yundan Liu, Hongbing Shao, Andrew Mcminn, Min Wang
Summary: This study isolated a novel virus, vB_VviC_ZQ26, infecting Vibrio cyclitrophicus from coastal waters near Qingdao, China. It was found that vB_VviC_ZQ26 belongs to a new vibriophage-specific family, Coheviridae, and is mainly found in temperate and tropical epipelagic zones.
Article
Virology
Xing Li, Zhiping Ye, Ewan P. Plant
Summary: By analyzing clinical and cultured samples using the same bioinformatic pipeline, it was found that 5' copyback DVGs are prevalent in human clinical samples but not in cultured samples. Furthermore, there are differences in DVG production and composition between in vivo and in vitro infections.
Article
Virology
Shreya Banerjee, Rakesh Sarkar, Arpita Mukherjee, Suvrotoa Mitra, Animesh Gope, Mamta Chawla-Sarkar
Summary: Rotavirus is a major cause of viral gastroenteritis in infants and continues to be a severe issue in underdeveloped nations. Recent research has shown that a long non-coding RNA called SLC7A11-AS1 is upregulated during rotavirus infection and plays a role in facilitating the virus's propagation by regulating intracellular glutathione and lipid peroxidation.
Article
Virology
Eiichi Hondo, Tetsufumi Katta, Ayato Sato, Naoya Kadofusa, Tomoki Ishibashi, Hiroshi Shimoda, Hirokazu Katoh, Atsuo Iida
Summary: Bat-borne emerging viruses, such as the Ebola virus and Nipah virus, pose a significant threat to public health. In this study, we identified micafungin as a potential antiviral drug against Pteropine orthoreovirus (PRV) and found that it inhibits viral release in human cells through activating the endoplasmic reticulum stress response pathway. This research provides valuable insights into the development of effective treatments for future virus pandemics.
Article
Virology
M. Joubert, N. van den Berg, J. Theron, V. Swart
Summary: This study investigated the host gene expression in asymptomatic avocado nursery trees infected with Avocado sunblotch viroid (ASBVd) using RNA sequencing. The results showed that 631 genes were differentially expressed during infection, with 63% upregulated. ASBVd infection significantly affected plant defence responses, phytohormone networks, gene expression pathways, secondary metabolism, cellular transport, as well as protein modification and degradation. This study provides insights into the molecular interactions underlying avsunviroid-host interactions.
Article
Virology
Wanling Zhang, Haiyan Yang, Zhengyun Liu, Shengyu Wang, Tianyang Chen, Hong Song, Yunbin Xu, Fajin Li, Guo Luo, Huan Wang
Summary: EV71 infection leads to changes in mitochondrial morphology and dynamics in human neuroblastoma cells, resulting in mitochondrial dysfunction that may contribute to nervous system dysfunction.
Article
Virology
Mikhail Oliveira Leastro, Elliot Watanabe Kitajima, Vicente Pallas, Jesus Angel Sanchez- Navarro
Summary: This study reports the construction and validation of an infectious cDNA clone of Citrus leprosis virus C (CiLV-C) based on an agroinfection system. Agro-injected Nicotiana benthamiana plants showed localized lesions similar to the natural infection process. The virus recovered from the infected plant tissue could be mechanically transmitted between plants. The CiLV-C reverse genetic system provides a powerful molecular tool for unraveling the peculiarities of the citrus leprosis pathosystem.
Article
Virology
Manel Essaidi-Laziosi, Francisco J. Perez-Rodriguez, Catia Alvarez, Pascale Sattonnet-Roche, Giulia Torriani, Meriem Bekliz, Kenneth Adea, Matthias Lenk, Tasnim Suliman, Wolfgang Preiser, Marcel A. Mueller, Christian Drosten, Laurent Kaiser, Isabella Eckerle
Summary: This study found that the Omicron variant has different replication characteristics compared to other variants of concern, being able to efficiently produce infectious virus in nasal cells but not lung cells. In addition, the Delta and Omicron variants showed increased infection efficiency in A549 cells. These findings suggest that the variants of concern have better adaptation to humans but do not have an extended host range.
Article
Virology
Martin Faye, Modeste Name Faye, Babacar Ndiaye, Moussa Moise Diagne, Safietou Sankhe, Ndeye Marieme Top, Amadou Diallo, Cheikh Loucoubar, Ndongo Dia, Amadou Alpha Sall, Ousmane Faye
Summary: Genomic surveillance in Senegal since March 2020 has detected the emergence of Omicron BA.4 and BA.5 sublineages in June 2022. Next-generation sequencing and phylogeny analysis of isolates from a cluster of cases in Northern Senegal in July 2022 revealed that the BA.4 cases originated from a XAS recombinant, marking the first reported sequence of this variant from Senegal. Continuous genomic surveillance of positive SARS-CoV-2 samples is crucial.
