期刊
VIRUS RESEARCH
卷 178, 期 2, 页码 328-339出版社
ELSEVIER
DOI: 10.1016/j.virusres.2013.09.024
关键词
African swine fever; ASFV; Transmission; Challenge; Pathogenesis; Domestic pig
类别
资金
- Agricultural Research Service-Current Research Information System [1940-32000-056-00D]
- Science and Technology Directorate of the U.S. Department of Homeland Security [HSHQPM-12-X-00005]
- Plum Island Animal Disease Research Participation Program
To investigate the pathogenesis of African swine fever virus (ASFV), domestic pigs (n = 18) were challenged with a range (10(2)-10(6) 50% hemadsorbing doses (HAD(50))) of the highly virulent ASFV-Malawi strain by inoculation via the intraoropharyngeal (IOP), intranasopharyngeal (INP), or intramuscular (IM) routes. A subsequent contact challenge experiment was performed in which six IOP-inoculated donor pigs were allowed to have direct contact (DC) with six naive pigs for exposure times that varied from 24 to 72 h. All challenge routes resulted in clinical progression and postmortem lesions similar to those previously described in experimental and natural infection. The onset of clinical signs occurred between 1 and 7 days post inoculation (dpi) and included pyrexia with variable progression to obtundation, hematochezia, melena, moribundity and death with a duration of 4-11 days. Viremia was first detected between 4 and 5 dpi in all inoculation groups whereas ASFV shedding from the nasal cavity and tonsil was first detected at 3-9 dpi. IM and DC were the most consistent modes of infection, with 12/12 (100%) of pigs challenged by these routes becoming infected. Several clinical and virological parameters were significantly different between IM and DC groups indicating dissimilarity between these modes of infection. Amongst the simulated natural routes, INP inoculation resulted in the most consistent progression of disease across the widest range of doses whilst preserving simulation of natural exposure and therefore may provide a superior system for pathogenesis and vaccine efficacy investigation. Published by Elsevier B.V.
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