Article
Horticulture
Abhinav Kumar, Nishtha Kashyap, Rohit Gupta
Summary: Papaya leaf curl disease poses a serious threat to papaya production in India, and can also infect chili and tomato plants. The study identified a virus from infected papaya plants that shared nucleotide sequences with chili leaf curl virus and tomato leaf curl virus in different parts of India. Additionally, the study demonstrated biological and molecular characteristics of a recombinant ChiLCV isolated from papaya plants.
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
(2021)
Article
Plant Sciences
Cristina Saez, Amina Kheireddine, Arcadio Garcia, Alicia Sifres, Alejandro Moreno, Maria Isabel Font-San-Ambrosio, Belen Pico, Carmelo Lopez
Summary: This study examined the distribution of ToLCNDV in different parts of infected plants and evaluated its transmission through seeds. Although the virus was found in the seeds, there was no evidence to support its transmission from contaminated seeds to the offspring.
Article
Biochemical Research Methods
Chi Yao, Rui Zhang, Jianpu Tang, Dayong Yang
Summary: DNA hydrogels have unique properties and can be synthesized efficiently using rolling circle amplification.
Article
Biochemical Research Methods
Monika Janik, Seyed Vahid Hamidi, Marcin Koba, Jonathan Perreault, Ryan Walsh, Wojtek J. Bock, Mateusz Smietana
Summary: This study demonstrates the potential of utilizing an optical fiber-fabricated unlabeled microcavity in-line Mach-Zehnder interferometer for real-time monitoring of DNA isothermal amplification, showing promising application in disease diagnosis.
Article
Virology
Rafaela S. Fontenele, Amulya Bhaskara, Ilaria N. Cobb, Lucas C. Majure, Andrew M. Salywon, Jesus A. Avalos-Calleros, Gerardo R. Arguello-Astorga, Kara Schmidlin, Philippe Roumagnac, Simone G. Ribeiro, Simona Kraberger, Darren P. Martin, Pierre Lefeuvre, Arvind Varsani
Summary: The study identified Squash leaf curl virus (SLCV) and watermelon chlorotic stunt virus (WCSV) in samples from Cactaceae, Solanaceae, Cucurbitaceae, and Lamiaceae plants. The detection of these viruses in cactus plants suggests possible spillover events from agricultural areas into native vegetation. The study also highlights the need for more surveillance of WCSV in the USA as it poses a serious threat to watermelon cultivation.
Article
Biochemical Research Methods
S. P. Gawande, K. P. Raghavendra, D. Monga, D. T. Nagrale, T. Prabhulinga, N. Hiremani, M. Meshram, Sandhya Kranthi, Nandini Gokte-Narkhedkar, V. N. Waghmare
Summary: This study standardized a rapid and sensitive LAMP protocol for the detection of cotton leaf curl virus in cotton leaves and whiteflies. The technique has high practicality and can overcome diagnostic failures due to low virus titres.
JOURNAL OF VIROLOGICAL METHODS
(2022)
Article
Plant Sciences
Ho-Hsiung Chang, Deri Gustian, Chung-Jan Chang, Fuh-Jyh Jan
Summary: Understanding the seedborne nature of plant viruses is crucial for disease control strategies and seed market. This study revealed that ToLCNDV, ToLCTV, and TYLCTHV can be transmitted through seeds or pollens of cucumber and tomato plants.
Article
Plant Sciences
V. Venkataravanappa, K. Ashwathappa, H. D. Vinaykumar, C. N. Lakshminarayana Reddy, K. S. Shankarappa, M. Krishna Reddy
Summary: Crotalaria juncea L., known as sunhemp, is a tropical legume plant with good potential for nitrogen fixation and drought tolerance. However, it can act as an alternative host for begomoviruses, with sequencing analysis showing a high sequence identity between the virus and associated DNA satellites.
JOURNAL OF PLANT PATHOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Ying Cao, Na Wu, Hui-Da Li, Jing-Wen Xue, Rui Wang, Ting Yang, Jian-Hua Wang
Summary: A dynamic deformable nanointerface is fabricated using the unique features of the M13 phage for ultrasensitive detection of pathogens, showing superior capture affinity and fast amplification efficiency.
Article
Cell Biology
Moshik Shteinberg, Ritesh Mishra, Ghandi Anfoka, Miassar Altaleb, Yariv Brotman, Menachem Moshelion, Rena Gorovits, Henryk Czosnek
Summary: Research indicates a positive interaction between viruses and plants, with Tomato yellow curl virus (TYLCV) enhancing tolerance to drought in tomato plants. TYLCV-resistant tomato plants infected with the virus can survive long periods without water and produce fruits similar to control plants after recovery.
Article
Chemistry, Physical
Yoonbin Ji, Jong Bum Lee
Summary: This study introduces a method for fabricating DNA-iron oxide hybrid structures through co-precipitation, producing porous and anionic DNA microsponges. These structures show that DNA can be combined with iron oxide nanoparticles without agglomeration, demonstrating the potential for DNA-templated hybrid structures with metallic materials.
APPLIED SURFACE SCIENCE
(2021)
Article
Plant Sciences
Mariarosaria Mastrochirico, Roberta Spano, Tiziana Mascia
Summary: Tomato leaf curl New Delhi virus (ToLCNDV) is a newly emerging begomovirus that causes serious production losses in cucurbits. Grafting is a faster and more flexible solution compared to genetic resistance, as it induces tolerance against airborne virus infection. Through screening, potential rootstocks have been identified to achieve suitable levels of tolerance against the virus in commercial cucurbit varieties.
Article
Agronomy
Nasim Ahmed, Syed Shan-e-Ali Zaidi, Imran Amin, Brian E. Scheffler, Shahid Mansoor
Summary: The global spread of Tomato yellow leaf curl virus poses a threat to tomato production in tropical and sub-tropical regions, with a new species Tomato leaf curl Oman virus identified in Oman. In Pakistan, Tomato leaf curl disease symptoms were observed in tomato plants, with the presence of ToLCOMV-PK and associated ToLCB causing enhanced symptoms and virus accumulation in Nicotiana benthamiana plants.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2021)
Article
Plant Sciences
Thuy T. B. Vo, Aamir Lal, Phuong T. Ho, Elisa Troiano, Giuseppe Parrella, Eui-Joon Kil, Sukchan Lee
Summary: Tomato leaf curl New Delhi virus (ToLCNDV) has caused significant damage to Cucurbitaceae cultivation in Europe from 2017 to 2020. This study identified a new isolate of ToLCNDV in Pakistan and analyzed its infectivity in different cucurbit crops. The results showed severe infection in most cucurbit crops, indicating the potential resistance of watermelon cultivars to ToLCNDV. These findings provide valuable information for breeding resistance to ToLCNDV in Southeast Asia and the Mediterranean region.
Article
Biotechnology & Applied Microbiology
V. Venkataravanappa, C. N. Lakshminarayana Reddy, M. Nandan, Shridhar Hiremath, K. Ashwathappa, K. S. Shankarappa, H. D. Vinay Kumar, M. Krishna Reddy
Summary: The study revealed severe infection of summer squash by begomoviruses, primarily transmitted through whiteflies. Female insects were found to transmit the virus more efficiently than male insects. The virus was sequenced and identified through methods such as PCR and rolling circle amplification, showing close relation to the Indian strain of Squash leaf curl China virus.
Article
Multidisciplinary Sciences
Ana Moreno-Delafuente, Ignacio Morales, Elisa Garzo, Alberto Fereres, Elisa Vinuela, Pilar Medina
Summary: Elevated CO2 levels can alter plant metabolism and affect herbivore insect performance. The study found that melon plants grown under eCO2 had lower nitrogen foliar concentration, higher carbon to nitrogen ratio, decreased amino acid content, and increased sucrose content. This led to reductions in population growth and body mass of Aphis gossypii, highlighting a negative response of this pest to eCO2 in a global change scenario.
SCIENTIFIC REPORTS
(2021)
Article
Entomology
Jacinto Benhadi-Marin, Luis F. Pereira, Isabel Rodrigues, Alberto Fereres, Jose Alberto Pereira
Summary: Efficient sampling protocols are essential for monitoring crop pests, especially in the case of the African citrus psyllid T. erytreae that threatens the lemon industry. Yellow sticky traps were found to be the most cost-effective method, with at least three traps per hectare providing 90% accuracy in estimating the population during outbreaks. These results can help monitor and anticipate pest outbreaks that may spread to neighboring orchards.
JOURNAL OF APPLIED ENTOMOLOGY
(2021)
Article
Entomology
M. Godefroid, M. Morente, T. Schartel, D. Cornara, A. Purcell, D. Gallego, A. Moreno, J. A. Pereira, A. Fereres
Summary: Xf, an invasive pathogen spread by insects, is causing severe outbreaks in the Mediterranean region. Models predict that Western Europe and the Mediterranean basin are highly suitable for the vector responsible for Xf spread. However, the southern Mediterranean area may experience a decrease in suitability for the vector in the future.
JOURNAL OF PEST SCIENCE
(2022)
Article
Entomology
Matteo Ripamonti, Federico Maron, Daniele Cornara, Cristina Marzachi, Alberto Fereres, Domenico Bosco
Summary: The feeding behavior of Scaphoideus titanus on different grape varieties was studied, revealing differences in the duration of the phloem feeding phase and the frequency of interruption-salivation events. The preference of the leafhopper for the Barbera cultivar correlated with an enhanced transmission efficiency of FD, explaining the higher susceptibility of this variety to the disease.
JOURNAL OF INSECT PHYSIOLOGY
(2022)
Article
Entomology
Jacinto Benhadi-Marin, Alberto Fereres, Jose Alberto Pereira
Summary: This study identified key risk areas and potential corridors for colonization by the psyllid vector Trioza erytreae in the Iberian Peninsula, focusing on citrus-growing areas in Setubal and Huelva, as well as potential connections between Guipuzcoa and the Ebro valley. Future modeling studies should consider biological constraints and other barriers or corridors when assessing the spread of this vector.
JOURNAL OF PEST SCIENCE
(2022)
Article
Multidisciplinary Sciences
Isabel Rodrigues, Vitor Ramos, Jacinto Benhadi-Marin, Aranzazu Moreno, Alberto Fereres, Jose Alberto Pereira, Paula Baptista
Summary: A PCR-based approach was developed to detect Philaenus spumarius DNA in spider's gut. Two primer sets showed high specificity and sensitivity, amplifying specific fragments with low concentration of Philaenus spumarius DNA. The feasibility of detecting predation of Philaenus spumarius by spiders was confirmed in the field.
SCIENTIFIC REPORTS
(2022)
Article
Agronomy
Marina Morente, Marcos Ramirez, Clara Lago, Daniel de las Heras-Bravo, Alejandro Benito, Aranzazu Moreno, Alberto Fereres
Summary: The study assessed the impact of common plant species used as cover crops on the population growth of Philaeus spumarius. A series of experiments were conducted in laboratory and semi-field conditions. The findings contribute to the design of ecological infrastructures for effective management of Xf vectors.
PEST MANAGEMENT SCIENCE
(2022)
Article
Agronomy
Diogo Felix Oliveira, Jacinto Benhadi-Marin, Joana Neto, Lorena Sanz, Elisa Garzo, Ana Aguiar, Alberto Fereres, Jose Alberto Pereira
Summary: The use of kaolin was found to significantly reduce the number of Trioza erytreae on lemon plants and shorten their feeding duration. This suggests that kaolin could be an effective tool in integrated pest management programs or organic production to limit the spread of HLB in citrus crops.
PEST MANAGEMENT SCIENCE
(2022)
Article
Agronomy
Clara Lago, Daniele Cornara, Serena Anna Minutillo, Aranzazu Moreno, Alberto Fereres
Summary: This study analyzed the lethal and sublethal effects of six commercial products on Philaenus spumarius and assessed their impact on the transmission of X. fastidiosa. The results showed that deltamethrin and acetamiprid significantly altered the feeding behavior of P. spumarius, while spinosad, sulfoxaflor, and kaolin did not. Pyrethrin was the only compound able to reduce X. fastidiosa transmission under both free-choice and no-choice conditions.
PEST MANAGEMENT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Imre Mezei, Pablo Valverde-Garcia, Melissa W. Siebert, Luis E. Gomez, Maria Torne, Gerald B. Watson, Abad M. Raquel, Alberto Fereres, Thomas C. Sparks
Summary: This study confirms the high efficacy of sulfoxaflor in controlling peach aphids. However, it also reveals that there is no correlation between the resistance ratios of imidacloprid and sulfoxaflor for colonies with the R81T mutation. Therefore, it is recommended to implement insecticide resistance management measures when using sulfoxaflor.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Maeelle Deshoux, Baptiste Monsion, Elodie Pichon, Jaime Jimenez, Aranzazu Moreno, Bastien Cayrol, Gael Thebaud, Sam T. Mugford, Saskia A. Hogenhout, Stephane Blanc, Alberto Fereres, Marilyne Uzest
Summary: To ensure sustained feeding, aphids deliver effectors into plant cells using their mouthparts, avoiding the activation of plant defenses. Recent research has shown that the distribution of effectors near feeding sites in plant tissues is uneven. However, the specific process of delivering effectors into specific plant compartments is still unknown. This study demonstrates that the acrostyle, a cuticular organ located at the tip of maxillary stylets, binds with a saliva effector called Mp10 during aphid probing. The acrostyle's capacity to bind with effectors supports its role in delivering aphid effectors into plant cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Entomology
Anna Markheiser, Giacomo Santoiemma, Alberto Fereres, Sanela Kugler, Michael Maixner, Daniele Cornara
Summary: In this study, the feeding behavior of four xylem-feeding insect species on grapevines was compared using the DC-EPG technique. The results showed significant differences in probing frequency and dynamics of xylem ingestion between sharpshooters and spittlebugs. The green leafhopper Cicadella viridis displayed the highest frequency of the electrical pattern associated with X. fastidiosa inoculation in spittlebugs.
JOURNAL OF APPLIED ENTOMOLOGY
(2023)
Article
Plant Sciences
Bernard Reynaud, Patrick Turpin, Florencia M. Molinari, Martial Grondin, Solene Roque, Frederic Chiroleu, Alberto Fereres, Helene Delatte
Summary: Huanglongbing (HLB) is a serious disease of citrus worldwide, caused by three non-cultivable bacteria. The African psyllid Trioza erytreae is capable of efficiently acquiring and transmitting the bacteria, highlighting the high risk of spread of the disease in Europe.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biology
Alex Gimenez-Romero, Javier Galvan, Marina Montesinos, Joan Bauza, Martin Godefroid, Alberto Fereres, Jose J. Ramasco, Manuel A. Matias, Eduardo Moralejo
Summary: This study utilizes a dynamic epidemiological model to assess the potential incidence of Pierce's disease (PD), a lethal grapevine disease caused by the bacterium Xylella fastidiosa. By integrating data from inoculation assays and vector distribution, along with high-resolution spatiotemporal climatic data, the study predicts a global expansion of epidemic-risk zones for PD by 2050.
COMMUNICATIONS BIOLOGY
(2022)
Article
Entomology
Clara Lago, Alex Gimenez-Romero, Marina Morente, Manuel A. Matias, Aranzazu Moreno, Alberto Fereres
Summary: This study built a growing degree day (GDD) model based on daily temperatures and relative humidities to predict the time of egg hatching of Philaenus spumarius in the Iberian Peninsula. The model was validated with field observations and used as a decision-support tool for applying control actions against the insect pest. Controlling nymphs at two different dates could target the highest percentages of nymphal populations.
ENVIRONMENTAL ENTOMOLOGY
(2023)
Article
Virology
Mohamed S. H. Hassan, Ahmed Ali, Motamed Elsayed Mahmoud, Danah Altakrouni, Shahnas M. Najimudeen, Mohamed Faizal Abdul-Careem
Summary: This study compared the efficacy of different vaccination programs against Canadian DMV/1639 IBV and found that the autogenous inactivated DMV/1639 vaccine was more effective in reducing viral loads and boosting virus neutralization titers. Both vaccination programs provided significant protection against pathological effects.
Article
Virology
Wanda Christ, Jonas Klingstrom, Janne Tynell
Summary: The integrated stress response (ISR) is activated in response to SARS-CoV-2 infection, triggering translational arrest and inhibiting stress granule formation. However, the stress-responsive transcription factors ATF4 and CHOP are not induced. Different SARS-CoV-2 variants show variant-specific differences in ISR activation, suggesting potential impacts on pathogenesis and treatment strategies.
Article
Virology
Xiao Han, Hankun Xu, Yifan Weng, Rong Chen, Jidong Xu, Tong Cao, Renjie Sun, Ying Shan, Fang He, Weihuan Fang, Xiaoliang Li
Summary: Classical swine fever virus (CSFV) can inhibit the host innate immunity by decreasing the level of high mobility group box 1 (HMGB1), and CSFV Npro can evade host antiviral immune response through upregulating HMGB1 acetylation.
Article
Virology
Sunjian Lyu, Fulei Xiong, Tianpeng Qi, Weifeng Shen, Qi Guo, Mingming Han, Li Liu, Weishao Bu, Julin Yuan, Bao Lou
Summary: This study identified and characterized a novel temperate A. hydrophila phage, P05B, which has strong lytic ability and stability across different temperatures and pH values.
Article
Virology
Siyuan Liu, Wei Chen, Raphael Nyaruaba, Shunlong Wang, Cihan Yang, Qun Wu, Ying Liu, Puyu Liu, Fei Wang, Jingling Wang, Zhiming Yuan, Dingwei Sun, Han Xia
Summary: Specific, sensitive, and reliable RT-qPCR assays for the detection of OYAV and EBIV have been successfully developed in this study. These assays hold great promise for their potential application in clinical and field samples in the future.
Article
Virology
Rui Wang, Qiang Sun, Jinbo Xiao, Congcong Wang, Xiaoliang Li, Jichen Li, Yang Song, Huanhuan Lu, Ying Liu, Shuangli Zhu, Zhijun Liu, Yong Zhang
Summary: Coxsackievirus A6 (CVA6) has emerged as an important agent causing hand, foot, and mouth disease (HFMD). Mutations in residue 64 of the virus have been found to increase resistance to ribavirin and decrease sensitivity to guanidine hydrochloride. This mutation also leads to increased replication fidelity and reduced pathogenicity. These findings provide a foundation for the development of a safe and effective live attenuated CVA6 vaccine.
Article
Virology
Yao Xiong, Keran Ma, Xiao Zou, Yantao Liang, Kaiyang Zheng, Tiancong Wang, Hong Zhang, Yue Dong, Ziyue Wang, Yundan Liu, Hongbing Shao, Andrew Mcminn, Min Wang
Summary: This study isolated a novel virus, vB_VviC_ZQ26, infecting Vibrio cyclitrophicus from coastal waters near Qingdao, China. It was found that vB_VviC_ZQ26 belongs to a new vibriophage-specific family, Coheviridae, and is mainly found in temperate and tropical epipelagic zones.
Article
Virology
Xing Li, Zhiping Ye, Ewan P. Plant
Summary: By analyzing clinical and cultured samples using the same bioinformatic pipeline, it was found that 5' copyback DVGs are prevalent in human clinical samples but not in cultured samples. Furthermore, there are differences in DVG production and composition between in vivo and in vitro infections.
Article
Virology
Shreya Banerjee, Rakesh Sarkar, Arpita Mukherjee, Suvrotoa Mitra, Animesh Gope, Mamta Chawla-Sarkar
Summary: Rotavirus is a major cause of viral gastroenteritis in infants and continues to be a severe issue in underdeveloped nations. Recent research has shown that a long non-coding RNA called SLC7A11-AS1 is upregulated during rotavirus infection and plays a role in facilitating the virus's propagation by regulating intracellular glutathione and lipid peroxidation.
Article
Virology
Eiichi Hondo, Tetsufumi Katta, Ayato Sato, Naoya Kadofusa, Tomoki Ishibashi, Hiroshi Shimoda, Hirokazu Katoh, Atsuo Iida
Summary: Bat-borne emerging viruses, such as the Ebola virus and Nipah virus, pose a significant threat to public health. In this study, we identified micafungin as a potential antiviral drug against Pteropine orthoreovirus (PRV) and found that it inhibits viral release in human cells through activating the endoplasmic reticulum stress response pathway. This research provides valuable insights into the development of effective treatments for future virus pandemics.
Article
Virology
M. Joubert, N. van den Berg, J. Theron, V. Swart
Summary: This study investigated the host gene expression in asymptomatic avocado nursery trees infected with Avocado sunblotch viroid (ASBVd) using RNA sequencing. The results showed that 631 genes were differentially expressed during infection, with 63% upregulated. ASBVd infection significantly affected plant defence responses, phytohormone networks, gene expression pathways, secondary metabolism, cellular transport, as well as protein modification and degradation. This study provides insights into the molecular interactions underlying avsunviroid-host interactions.
Article
Virology
Wanling Zhang, Haiyan Yang, Zhengyun Liu, Shengyu Wang, Tianyang Chen, Hong Song, Yunbin Xu, Fajin Li, Guo Luo, Huan Wang
Summary: EV71 infection leads to changes in mitochondrial morphology and dynamics in human neuroblastoma cells, resulting in mitochondrial dysfunction that may contribute to nervous system dysfunction.
Article
Virology
Mikhail Oliveira Leastro, Elliot Watanabe Kitajima, Vicente Pallas, Jesus Angel Sanchez- Navarro
Summary: This study reports the construction and validation of an infectious cDNA clone of Citrus leprosis virus C (CiLV-C) based on an agroinfection system. Agro-injected Nicotiana benthamiana plants showed localized lesions similar to the natural infection process. The virus recovered from the infected plant tissue could be mechanically transmitted between plants. The CiLV-C reverse genetic system provides a powerful molecular tool for unraveling the peculiarities of the citrus leprosis pathosystem.
Article
Virology
Manel Essaidi-Laziosi, Francisco J. Perez-Rodriguez, Catia Alvarez, Pascale Sattonnet-Roche, Giulia Torriani, Meriem Bekliz, Kenneth Adea, Matthias Lenk, Tasnim Suliman, Wolfgang Preiser, Marcel A. Mueller, Christian Drosten, Laurent Kaiser, Isabella Eckerle
Summary: This study found that the Omicron variant has different replication characteristics compared to other variants of concern, being able to efficiently produce infectious virus in nasal cells but not lung cells. In addition, the Delta and Omicron variants showed increased infection efficiency in A549 cells. These findings suggest that the variants of concern have better adaptation to humans but do not have an extended host range.
Article
Virology
Martin Faye, Modeste Name Faye, Babacar Ndiaye, Moussa Moise Diagne, Safietou Sankhe, Ndeye Marieme Top, Amadou Diallo, Cheikh Loucoubar, Ndongo Dia, Amadou Alpha Sall, Ousmane Faye
Summary: Genomic surveillance in Senegal since March 2020 has detected the emergence of Omicron BA.4 and BA.5 sublineages in June 2022. Next-generation sequencing and phylogeny analysis of isolates from a cluster of cases in Northern Senegal in July 2022 revealed that the BA.4 cases originated from a XAS recombinant, marking the first reported sequence of this variant from Senegal. Continuous genomic surveillance of positive SARS-CoV-2 samples is crucial.
