Article
Veterinary Sciences
Onyekachukwu H. Osemeke, Guilherme A. Cezar, Rodrigo C. Paiva, Daniel C. A. Moraes, Isadora F. Machado, Edison S. Magalhaes, Ana Paula S. Poeta Silva, Mafalda Mil-Homens, Li Peng, Swaminathan Jayaraman, Giovani Trevisan, Gustavo S. Silva, Phillip C. Gauger, Daniel C. L. Linhares
Summary: This study compares the detection rates of PRRSV RNA in different sample types and evaluates the effect of pooling on the detection of PRRSV RNA. The results show that ES, NS, and OS can be used as samples for monitoring PRRSV infection in weaning-age pigs.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Veterinary Sciences
M. N. Almeida, M. Zhang, J. J. Zimmerman, D. J. Holtkamp, D. C. L. Linhares
Summary: This study compared the detection of PRRSV in commercial swine breeding herds using family oral fluids (FOF) and individual piglet serum samples, finding that FOF was an effective sample type for PRRSV detection in farrowing rooms. A risk-based approach for litter selection combined with FOF collection can improve on-farm PRRSV detection efficiency. The BayesPEM analysis showed that caution should be taken when interpreting negative surveillance results as PRRSV may still be present in breeding herds.
PREVENTIVE VETERINARY MEDICINE
(2021)
Article
Veterinary Sciences
Onyekachukwu Henry Osemeke, Nathan VanKley, Claire LeFevre, Christina Peterson, Daniel C. L. Linhares
Summary: This study compared the PRRSV test results of oral swabs and serum samples from weaning-age piglets and found a higher positivity rate in serum samples. Oral swabs can be used for PRRSV surveillance as a convenient sampling method, but negative results should be interpreted with caution.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Veterinary Sciences
Onyekachukwu H. Osemeke, Eduardo de Freitas Costa, Marcelo N. Almeida, Giovani Trevisan, Arka P. Ghosh, Gustavo S. Silva, Daniel C. L. Linhares
Summary: Family oral fluids (FOFs) are a cost-efficient and convenient sample type for determining the PRRSV status of weaning age pigs. This study shows that pooling PRRSV-positive FOF samples can affect the probability of PRRSV RNA detection, and that different pooling levels should be used based on the prevalence of PRRSV. The study also suggests that subsampling a farrowing room for FOF pools can be a cost-saving strategy.
PREVENTIVE VETERINARY MEDICINE
(2022)
Article
Veterinary Sciences
Arnaud Lebret, Valerie Normand, Pauline Berton, Theo Nicolazo, Charlotte Teixeira Costa, Celine Chevance, Mathieu Brissonnier, Gwenael Boulbria
Summary: The aim of this study was to compare the detection rate of PRRSV-1 using PCR in different sample types and evaluate the suitability of pooling. The results showed that blood samples had the highest detection rate, while family oral fluid and udder wipe samples had lower detection rates.
VETERINARY SCIENCES
(2023)
Article
Veterinary Sciences
Alexandra Henao-Diaz, Min Zhang, Luis Gimenez-Lirola, Esteban Ramirez, Phil Gauger, David H. Baum, Maria J. Clavijo, Marisa Rotolo, Rodger G. Main, Jeffrey Zimmerman
Summary: This study showed that setting the cut-off for PRRSV oral fluid antibody ELISA at S/P > 1.0 improved diagnostic specificity while minimally impacting diagnostic sensitivity. The manufacturer's recommended cut-off (S/P > 0.4) is suitable for diagnostic applications, but a higher cut-off is needed for surveillance purposes. Additionally, the study found a significant association between unexpected reactors and specific production sites and animal ages or stages, suggesting potential influences of certain animal husbandry or production practices on test results.
PREVENTIVE VETERINARY MEDICINE
(2021)
Article
Infectious Diseases
Benjamin D. Anderson, Myagmarsukh Yondon, Emily S. Bailey, Ege K. Duman, Ryan A. Simmons, Annette G. Greer, Gregory C. Gray
Summary: This study implemented a non-invasive bioaerosol sampling strategy in a swine farm in the United States to detect economically important swine pathogens. The results showed that the bioaerosol surveillance strategy was feasible and able to quickly generate data for dissemination to farm stakeholders.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Veterinary Sciences
Berenice Munguia-Ramirez, Betsy Armenta-Leyva, Alexandra Henao-Diaz, Ting-Yu Cheng, Jianqiang Zhang, Gaurav Rawal, Fangshu Ye, Luis Gimenez-Lirola, Jeffrey J. Zimmerman
Summary: We investigated the effects of temperature x time, freeze-thaw cycles, and high PRRSV RNA concentrations on the detection of PRRSV and ISC in different sample types. Serum samples should be stored at <=20 degrees C for optimal PRRSV RNA detection, while oral fluid and fecal samples should be frozen until tested. Freeze-thaw cycles had little impact on detection, but should be minimized in oral fluid samples.
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2023)
Article
Veterinary Sciences
A. Lebret, P. Berton, V. Normand, I. Messager, N. Robert, F. Bouchet, M. Brissonnier, G. Boulbria
Summary: In France, PRRSV stabilization protocols have been implemented using mass vaccination with a modified live vaccine, herd closure and biosecurity measures. The use of processing fluid for PRRSV monitoring is more sensitive than blood samples. Data on vertical transmission of MLV viruses are lacking.
PORCINE HEALTH MANAGEMENT
(2021)
Article
Infectious Diseases
Cristina Aira Pino, Maren Penning, Martin Eiden, Anne Balkema-Buschmann, Sandra Blome, Katrin Strutzberg-Minder, Lissett Lopez, Paloma Rueda, Patricia Sastre
Summary: The livestock industry is crucial for the livelihood of around 1.3 billion people worldwide, with the swine industry contributing significantly. Continuous sero-surveillance is essential for targeted veterinary public health interventions and prevention strategies. Multiplex assays are powerful tools that can simplify surveillance programs.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Oncology
Fernando Lozano, Carles X. X. Raventos, Albert Carrion, Carme Dinares, Javier Hernandez, Enrique Trilla, Juan Morote
Summary: The urine biomarker XBM can predict early high-risk recurrences in non-muscle invasive bladder cancer (NMIBC), but it has low sensitivity and cannot replace cystoscopy and washing cytology.
Article
Veterinary Sciences
Will Lopez, Jeff Zimmerman, Phil Gauger, Karen Harmon, Ronaldo Magtoto, Laura Bradner, Derald Holtkamp, Min Zhang, Jianqiang Zhang, Alejandro Ramirez, Daniel Linhares, Luis Gimenez-Lirola
Summary: Surveillance of porcine reproductive and respiratory syndrome virus (PRRSV) is important for breeding herds, and processing fluids are commonly used for this purpose. However, the diagnostic characteristics of processing fluids are not well understood. In this study, we conducted three experiments and found that PRRSV RNA remains stable in processing fluid samples under certain storage conditions and that a modified PRRSV ELISA method can reliably detect PRRSV antibodies in these samples.
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2022)
Article
Infectious Diseases
Kalhari B. Goonewardene, Chungwon J. Chung, Melissa Goolia, Leslie Blakemore, Andrew Fabian, Fawzi Mohamed, Charles Nfon, Alfonso Clavijo, Kimberly A. Dodd, Aruna Ambagala
Summary: The study showed that pen-based oral fluid samples performed well in early detection of ASFV in animal experiments, suggesting they can be used to supplement traditional samples for non-invasive screening.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Veterinary Sciences
Marcelo Nunes de Almeida, Cesar A. Corzo, Jeffrey J. Zimmerman, Daniel Correia Lima Linhares
Summary: This study validated the effectiveness of using PF and FOF in commercial breeding farms to enhance PRRSV detection, finding that PRRSV detection is often sporadic and non-uniform, with both sampling methods complementing each other.
PORCINE HEALTH MANAGEMENT
(2021)
Article
Veterinary Sciences
M. N. Almeida, M. Zhang, W. A. L. Lopez, C. Vilalta, J. Sanhueza, C. A. Corzo, J. J. Zimmerman, D. C. L. Linhares
Summary: The study found that the distribution of PRRSV-positive piglets in modern farrowing rooms is non-uniform, with 2SS and RBS being more efficient than SRS.
PREVENTIVE VETERINARY MEDICINE
(2021)
Article
Veterinary Sciences
Edison S. Magalhaes, Jeffrey J. Zimmerman, Pete Thomas, Cesar A. A. Moura, Giovani Trevisan, Derald J. Holtkamp, Chong Wang, Christopher Rademacher, Gustavo S. Silva, Daniel C. L. Linhares
Summary: This study developed an automated framework to analyze the risk factors for swine wean-to-finish mortality by integrating data from multiple sources. The results showed that sow farm health and productivity were closely linked to subsequent mortality during the wean-to-finish phase.
PREVENTIVE VETERINARY MEDICINE
(2022)
Article
Cell Biology
Sanjana Mahadev Bhat, Nyzil Massey, Denusha Shrestha, Locke A. Karriker, Tomislav Jelesijevic, Chong Wang, Chandrashekhar Charavaryamath
Summary: Exposure to organic dust in agriculture can cause respiratory symptoms and loss of lung function. Mitochondrial dysfunction induced by organic dust may be related to the secretion of HMGB1. Neutralization of HMGB1 secreted by organic dust exposure can rescue mitochondrial dysfunction in human bronchial epithelial cells.
CELL AND TISSUE RESEARCH
(2022)
Article
Microbiology
Lu Yen, Ronaldo Magtoto, Juan Carlos Mora-Diaz, Jose Antonio Carrillo-Avila, Jianqiang Zhang, Ting-Yu Cheng, Precy Magtoto, Rahul K. Nelli, David H. Baum, Jeffrey J. Zimmerman, Luis G. Gimenez-Lirola
Summary: This study evaluated the potential of using the receptor-binding subunit of the PDCoV spike protein for specific antibody detection via an ELISA assay. The results showed that the S1 protein-based ELISA can be used for accurate detection of PDCoV infections, transfer of maternal antibodies, or active surveillance.
Article
Virology
Lu Yen, Juan Carlos Mora-Diaz, Rolf Rauh, William Nelson, Gino Castillo, Fangshu Ye, Jianqiang Zhang, David Baum, Jeffrey Zimmerman, Rahul Nelli, Luis Gimenez-Lirola
Summary: This study characterized the susceptibility and dynamic of porcine deltacoronavirus infection in grower pigs under experimental conditions. The study found that active surveillance based on systematic sampling and laboratory testing is critical for the accurate detection of subclinical circulation of PDCoV in pigs after weaning.
Article
Microbiology
Virginia Friedrichs, Darwin Reicks, Tobias Hasenfuss, Elisabeth Gerstenkorn, Jeffrey J. Zimmerman, Eric A. Nelson, Tessa Carrau, Paul Deutschmann, Julia Sehl-Ewert, Hanna Roszyk, Martin Beer, Jane Christopher-Hennings, Sandra Blome
Summary: African swine fever virus (ASFV) can efficiently spread through artificial insemination to female pigs and also to implanted embryos, highlighting the critical role that boar semen plays in ASFV transmission.
Article
Veterinary Sciences
Berenice Munguia-Ramirez, Betsy Armenta-Leyva, Alexandra Henao-Diaz, Ting-Yu Cheng, Jianqiang Zhang, Gaurav Rawal, Fangshu Ye, Luis Gimenez-Lirola, Jeffrey J. Zimmerman
Summary: We investigated the effects of temperature x time, freeze-thaw cycles, and high PRRSV RNA concentrations on the detection of PRRSV and ISC in different sample types. Serum samples should be stored at <=20 degrees C for optimal PRRSV RNA detection, while oral fluid and fecal samples should be frozen until tested. Freeze-thaw cycles had little impact on detection, but should be minimized in oral fluid samples.
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2023)
Article
Veterinary Sciences
Betsy Armenta-Leyva, Berenice Munguia-Ramirez, Luis G. Gimenez-Lirola, Xue Lin, Fangshu Ye, Jeffrey Zimmerman
Summary: Based on publications reporting improvements in real-time PCR (rtPCR) performance, this study compared different protocols for the detection of specific viruses in swine oral fluids (OFs). The results showed that standard extraction and amplification methods provided superior detection of the viruses compared to heat treatment or dilution followed by direct rtPCR.
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2023)
Article
Agriculture, Dairy & Animal Science
Justin Brown, Korakrit Poonsuk, Ting-Yu Cheng, Chris Rademacher, Erin Kalkwarf, Liying Tian, Lauren A. McKeen, Chong Wang, Luis Gimenez-Lirola, David Baum, Locke A. Karriker
Summary: Antibodies can be transmitted from a sow to her piglets through milk, and their levels can be monitored in the sow's serum to determine if they can provide protection for the piglets. This study compared two diagnostic assays for the detection of antibodies for a specific virus in pigs, and found moderate agreement between the assays. The high-throughput neutralization assay showed advantages over the fluorescent focus neutralization assay in terms of specificity and result discrimination.
Article
Veterinary Sciences
Berenice Munguia-Ramirez, Betsy Armenta-Leyva, Alexandra Henao-Diaz, Fangshu Ye, David H. Baum, Luis G. Gimenez-Lirola, Jeffrey J. Zimmerman
Summary: This study aimed to evaluate the consistency of endogenous reference gene expression and their interpretation in diagnostic specimens. Results showed that the reference gene can accurately monitor sample quality in swine specimens tested for PRRSV. Failure to detect the reference gene may indicate abnormalities in the sample or testing procedures.
VETERINARY SCIENCES
(2023)
Article
Veterinary Sciences
Betsy Armenta-Leyva, Berenice Munguia-Ramirez, Ting-Yu Cheng, Fangshu Ye, Alexandra Henao-Diaz, Luis G. Gimenez-Lirola, Jeffrey Zimmerman
Summary: This study demonstrates the use of a normalization method in real-time PCR assays to control for normal variation in sampling and testing. The results show that converting sample quantification cycles to efficiency standardized cycles improves the accuracy of the assay. The method is shown to have good diagnostic capability in commercial porcine reproductive and respiratory syndrome virus testing.
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2023)
Article
Agriculture, Dairy & Animal Science
Edison S. S. Magalhaes, Danyang Zhang, Chong Wang, Pete Thomas, Cesar A. A. Moura, Derald J. J. Holtkamp, Giovani Trevisan, Christopher Rademacher, Gustavo S. S. Silva, Daniel C. L. Linhares
Summary: In this study, a data-wrangling pipeline was built to integrate diverse and dispersed data streams collected from a swine production company, creating a master table that was used to predict the nursery mortality of groups of pigs. The Support Vector Machine model demonstrated the best overall prediction results, showing high accuracy in forecasting nursery mortality.
Article
Microbiology
Gaurav Rawal, Jianqiang Zhang, Patrick G. Halbur, Phillip C. Gauger, Chong Wang, Tanja Opriessnig
Summary: The pathogenicity of currently circulating PRCV variant isolates was compared to traditional PRCV isolates, and it was found that the variant isolates are more transmissible and pathogenic. Additionally, it was observed that infection with the variant isolates did not impact influenza A virus infection. These findings raise concerns about the evolution of PRCV towards more highly pathogenic and transmissible strains and emphasize the importance of monitoring such viruses.
Article
Veterinary Sciences
Menghang Wang, Ying Yu, Jianan Wu, Shujie Wang, Luis G. Gimenez-Lirola, Pablo Pineyro, Yu Wang, Hongliang Cui, Xijun He, Jeffrey J. Zimmerman, Yabin Tu, Xuehui Cai, Gang Wang
Summary: In this study, researchers isolated and characterized the PCV3-DB-1 strain in PK-15 cells. They observed PCV-like particles through electron microscopy and confirmed the replication of PCV3 in PK-15 cell culture using in situ hybridization RNA analysis. Phylogenetic analysis placed PCV3-DB-1 in the PCV3a clade based on its Cap protein characteristics.
VETERINARY SCIENCES
(2023)
Article
Virology
Gaurav Rawal, Marcelo N. Almeida, Phillip C. Gauger, Jeffrey J. Zimmerman, Fangshu Ye, Christopher J. Rademacher, Betsy Armenta Leyva, Berenice Munguia-Ramirez, Grzegorz Tarasiuk, Loni L. Schumacher, Ethan K. Aljets, Joseph T. Thomas, Jin-Hui Zhu, Jolie B. Trexel, Jianqiang Zhang
Summary: This study characterized the recently emerged PRRSV 1-4-4 L1C variant (L1C.5) and found it to be highly virulent with potential higher transmissibility. Compared to other virus strains, pigs infected with L1C.5 showed more severe symptoms and higher viral levels. Additionally, the study found that some PRRSV isolates can cause brain infection.
Article
Environmental Sciences
Peiyang Li, Jacek A. A. Koziel, Nubia Macedo, Jeffrey J. J. Zimmerman, Danielle Wrzesinski, Erin Sobotka, Mateo Balderas, William B. B. Walz, Reid Vincent Paris, Myeongseong Lee, Dongjie Liu, Bauyrzhan Yedilbayev, Brett C. C. Ramirez, William S. S. Jenks
Summary: Improving indoor air quality has become crucial during the COVID-19 pandemic. Research shows that adding UV-C light to air filtration devices can effectively eliminate viable airborne bacteria and remove particulate matter.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Virology
Mohamed S. H. Hassan, Ahmed Ali, Motamed Elsayed Mahmoud, Danah Altakrouni, Shahnas M. Najimudeen, Mohamed Faizal Abdul-Careem
Summary: This study compared the efficacy of different vaccination programs against Canadian DMV/1639 IBV and found that the autogenous inactivated DMV/1639 vaccine was more effective in reducing viral loads and boosting virus neutralization titers. Both vaccination programs provided significant protection against pathological effects.
Article
Virology
Wanda Christ, Jonas Klingstrom, Janne Tynell
Summary: The integrated stress response (ISR) is activated in response to SARS-CoV-2 infection, triggering translational arrest and inhibiting stress granule formation. However, the stress-responsive transcription factors ATF4 and CHOP are not induced. Different SARS-CoV-2 variants show variant-specific differences in ISR activation, suggesting potential impacts on pathogenesis and treatment strategies.
Article
Virology
Xiao Han, Hankun Xu, Yifan Weng, Rong Chen, Jidong Xu, Tong Cao, Renjie Sun, Ying Shan, Fang He, Weihuan Fang, Xiaoliang Li
Summary: Classical swine fever virus (CSFV) can inhibit the host innate immunity by decreasing the level of high mobility group box 1 (HMGB1), and CSFV Npro can evade host antiviral immune response through upregulating HMGB1 acetylation.
Article
Virology
Sunjian Lyu, Fulei Xiong, Tianpeng Qi, Weifeng Shen, Qi Guo, Mingming Han, Li Liu, Weishao Bu, Julin Yuan, Bao Lou
Summary: This study identified and characterized a novel temperate A. hydrophila phage, P05B, which has strong lytic ability and stability across different temperatures and pH values.
Article
Virology
Siyuan Liu, Wei Chen, Raphael Nyaruaba, Shunlong Wang, Cihan Yang, Qun Wu, Ying Liu, Puyu Liu, Fei Wang, Jingling Wang, Zhiming Yuan, Dingwei Sun, Han Xia
Summary: Specific, sensitive, and reliable RT-qPCR assays for the detection of OYAV and EBIV have been successfully developed in this study. These assays hold great promise for their potential application in clinical and field samples in the future.
Article
Virology
Rui Wang, Qiang Sun, Jinbo Xiao, Congcong Wang, Xiaoliang Li, Jichen Li, Yang Song, Huanhuan Lu, Ying Liu, Shuangli Zhu, Zhijun Liu, Yong Zhang
Summary: Coxsackievirus A6 (CVA6) has emerged as an important agent causing hand, foot, and mouth disease (HFMD). Mutations in residue 64 of the virus have been found to increase resistance to ribavirin and decrease sensitivity to guanidine hydrochloride. This mutation also leads to increased replication fidelity and reduced pathogenicity. These findings provide a foundation for the development of a safe and effective live attenuated CVA6 vaccine.
Article
Virology
Yao Xiong, Keran Ma, Xiao Zou, Yantao Liang, Kaiyang Zheng, Tiancong Wang, Hong Zhang, Yue Dong, Ziyue Wang, Yundan Liu, Hongbing Shao, Andrew Mcminn, Min Wang
Summary: This study isolated a novel virus, vB_VviC_ZQ26, infecting Vibrio cyclitrophicus from coastal waters near Qingdao, China. It was found that vB_VviC_ZQ26 belongs to a new vibriophage-specific family, Coheviridae, and is mainly found in temperate and tropical epipelagic zones.
Article
Virology
Xing Li, Zhiping Ye, Ewan P. Plant
Summary: By analyzing clinical and cultured samples using the same bioinformatic pipeline, it was found that 5' copyback DVGs are prevalent in human clinical samples but not in cultured samples. Furthermore, there are differences in DVG production and composition between in vivo and in vitro infections.
Article
Virology
Shreya Banerjee, Rakesh Sarkar, Arpita Mukherjee, Suvrotoa Mitra, Animesh Gope, Mamta Chawla-Sarkar
Summary: Rotavirus is a major cause of viral gastroenteritis in infants and continues to be a severe issue in underdeveloped nations. Recent research has shown that a long non-coding RNA called SLC7A11-AS1 is upregulated during rotavirus infection and plays a role in facilitating the virus's propagation by regulating intracellular glutathione and lipid peroxidation.
Article
Virology
Eiichi Hondo, Tetsufumi Katta, Ayato Sato, Naoya Kadofusa, Tomoki Ishibashi, Hiroshi Shimoda, Hirokazu Katoh, Atsuo Iida
Summary: Bat-borne emerging viruses, such as the Ebola virus and Nipah virus, pose a significant threat to public health. In this study, we identified micafungin as a potential antiviral drug against Pteropine orthoreovirus (PRV) and found that it inhibits viral release in human cells through activating the endoplasmic reticulum stress response pathway. This research provides valuable insights into the development of effective treatments for future virus pandemics.
Article
Virology
M. Joubert, N. van den Berg, J. Theron, V. Swart
Summary: This study investigated the host gene expression in asymptomatic avocado nursery trees infected with Avocado sunblotch viroid (ASBVd) using RNA sequencing. The results showed that 631 genes were differentially expressed during infection, with 63% upregulated. ASBVd infection significantly affected plant defence responses, phytohormone networks, gene expression pathways, secondary metabolism, cellular transport, as well as protein modification and degradation. This study provides insights into the molecular interactions underlying avsunviroid-host interactions.
Article
Virology
Wanling Zhang, Haiyan Yang, Zhengyun Liu, Shengyu Wang, Tianyang Chen, Hong Song, Yunbin Xu, Fajin Li, Guo Luo, Huan Wang
Summary: EV71 infection leads to changes in mitochondrial morphology and dynamics in human neuroblastoma cells, resulting in mitochondrial dysfunction that may contribute to nervous system dysfunction.
Article
Virology
Mikhail Oliveira Leastro, Elliot Watanabe Kitajima, Vicente Pallas, Jesus Angel Sanchez- Navarro
Summary: This study reports the construction and validation of an infectious cDNA clone of Citrus leprosis virus C (CiLV-C) based on an agroinfection system. Agro-injected Nicotiana benthamiana plants showed localized lesions similar to the natural infection process. The virus recovered from the infected plant tissue could be mechanically transmitted between plants. The CiLV-C reverse genetic system provides a powerful molecular tool for unraveling the peculiarities of the citrus leprosis pathosystem.
Article
Virology
Manel Essaidi-Laziosi, Francisco J. Perez-Rodriguez, Catia Alvarez, Pascale Sattonnet-Roche, Giulia Torriani, Meriem Bekliz, Kenneth Adea, Matthias Lenk, Tasnim Suliman, Wolfgang Preiser, Marcel A. Mueller, Christian Drosten, Laurent Kaiser, Isabella Eckerle
Summary: This study found that the Omicron variant has different replication characteristics compared to other variants of concern, being able to efficiently produce infectious virus in nasal cells but not lung cells. In addition, the Delta and Omicron variants showed increased infection efficiency in A549 cells. These findings suggest that the variants of concern have better adaptation to humans but do not have an extended host range.
Article
Virology
Martin Faye, Modeste Name Faye, Babacar Ndiaye, Moussa Moise Diagne, Safietou Sankhe, Ndeye Marieme Top, Amadou Diallo, Cheikh Loucoubar, Ndongo Dia, Amadou Alpha Sall, Ousmane Faye
Summary: Genomic surveillance in Senegal since March 2020 has detected the emergence of Omicron BA.4 and BA.5 sublineages in June 2022. Next-generation sequencing and phylogeny analysis of isolates from a cluster of cases in Northern Senegal in July 2022 revealed that the BA.4 cases originated from a XAS recombinant, marking the first reported sequence of this variant from Senegal. Continuous genomic surveillance of positive SARS-CoV-2 samples is crucial.
