Article
Fisheries
Minglan Guo, Jingguang Wei, Yongcan Zhou, Qiwei Qin
Summary: In this study, a protein called EcMKP-1 was cloned and found to play a critical role in combating Singapore grouper iridovirus (SGIV) infection. It regulates the host's immune response and suppresses viral replication. The results demonstrate the important functions of EcMKP-1 in antiviral immunity, JNK dephosphorylation, and anti-apoptosis during SGIV infection.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Fisheries
Yuexuan Wang, Suifeng Xu, Chengzong Han, Youhua Huang, Jingguang Wei, Shina Wei, Qiwei Qin
Summary: The molecular mechanism of curcumin against SGIV infection mainly includes suppressing cell apoptosis, inhibiting related signaling pathways, and promoting autophagy.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Yuexuan Wang, Suifeng Xu, Chengzong Han, Liqun Wang, Qi Zheng, Shaowen Wang, Youhua Huang, Shina Wei, Qiwei Qin
Summary: SGIV infection, a major cause of mortality and economic losses in grouper aquaculture industry, can be effectively inhibited by curcumin through multiple antiviral mechanisms both in vitro and in vivo. Curcumin impairs attachment of SGIV virion and regulates cellular immune and inflammatory responses related to the NF-kappa B signaling pathway. Additionally, curcumin plays a critical role in preventing SGIV infection through enhancing antioxidant capacity via the Nrf2/Keap1 signaling pathway. This study highlights the potential of curcumin as a candidate drug for treating SGIV infection.
Article
Fisheries
Jinpeng Chen, Liqun Wang, Jianling Huang, Xinshuai Li, Lingfeng Guan, Qing Wang, Min Yang, Qiwei Qin
Summary: This study cloned and characterized a novel type of MHC-I genotype, named EcMHC-IA*01, which plays an important role in antiviral immune response and apoptosis in fish.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Suifeng Xu, Yuexuan Wang, Chengzong Han, Yunxiang Jiang, Qiwei Qin, Shina Wei
Summary: The study revealed that cystatin F (CyF) plays a crucial role in fish virus infection by participating in innate immune responses. It inhibits the expression of viral genes and regulates apoptosis, as well as the production of inflammatory factors. CyF also modulates the activity of promoters related to interferon and NF-kappa B. These findings highlight the importance of CyF in DNA virus infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Yiqun Li, Yanan Liu, Yong Zhou, Wenzhi Liu, Yuding Fan, Nan Jiang, Mingyang Xue, Yan Meng, Lingbing Zeng
Summary: Chinese giant salamander Bid (AdBid) is a pro-apoptotic member of the Bcl-2 superfamily, with increased expression in muscle, kidney, and spleen after GSIV infection. Overexpression of AdBid enhances apoptotic process and viral replication during GSIV infection. This study contributes to a better understanding of iridovirus-induced apoptosis.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Fisheries
Suifeng Xu, Yuexuan Wang, Yunxiang Jiang, Chengzong Han, Qiwei Qin, Shina Wei
Summary: Cystatin A (CyA) in orange-spotted grouper plays a crucial role in fish virus infection by participating in innate immune response, regulating viral gene expression, and promoting apoptosis.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Virology
Yuexuan Wang, Honglin Han, Kecheng Zhu, Suifeng Xu, Chengzong Han, Yunxiang Jiang, Shina Wei, Qiwei Qin
Summary: In this study, Ec-Cathepsin D (Ec-CD) was identified from the orange-spotted grouper and its function in SGIV infection was investigated. The overexpression of Ec-CD inhibited SGIV replication, SGIV-induced apoptosis, and pro-inflammatory cytokine production. It also suppressed the activation of MAPK pathways.
Article
Fisheries
Honglin Han, Liqun Wang, Suifeng Xu, Shaowen Wang, Min Yang, Qiwei Qin, Shina Wei
Summary: The scavenger receptor class B type 1 (SR-B1) plays a crucial role in the immune response against viral infections. In this study, SR-B1 in the orange-spotted grouper was identified and characterized, showing significant up-regulation after infection with Singapore grouper iridovirus (SGIV), leading to inhibition of SGIV infection and enhanced antiviral innate immune response. Overexpression of SR-B1 also inhibited viral entry into host cells.
Article
Fisheries
Honglin Han, Liqun Wang, Suifeng Xu, Shaowen Wang, Min Yang, Chengzong Han, Qiwei Qin, Shina Wei
Summary: In this study, Ec-MARCO was identified and characterized in the orange-spotted grouper, and its important role in regulating the immune response against SGIV infection was demonstrated.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Shina Wei, Honglin Han, Suifeng Xu, Youhua Huang, Jingguang Wei, Qiwei Qin
Summary: This study identified and characterized Ec-CysB in orange-spotted grouper, which plays a crucial role in the immune response. The results suggest that Ec-CysB affects viral replication and virus-induced cell apoptosis.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Cell Biology
Yun-Bin Lee, Minkyo Jung, Jeesoo Kim, Afandi Charles, Wanda Christ, Jiwoong Kang, Myeong-Gyun Kang, Chulhwan Kwak, Jonas Klingstrom, Anna Smed-Sorensen, Jong-Seo Kim, Ji Young Mun, Hyun-Woo Rhee
Summary: This study used a super-resolution proximity labeling (SR-PL) method to investigate the interactions between SARS-CoV-2 and host cells. The results showed that RNF5 interacts with ORF3a and promotes its protein degradation, leading to a reduction in SARS-CoV-2 infection rate.
Article
Fisheries
Pin -Hong Li, Jia-Yang He, Yi-Jie Cai, Yu-Si Wei, Xiang-Long Zhu, Jia-Deng-Hui Yang, Shi-Qi Yang, Sheng Zhou, Qi-Wei Qin, Hong-Yan Sun
Summary: Sec6, a component of the exocyst complex, plays important roles in cellular functions and SGIV infection, and regulates apoptosis.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Immunology
Luhao Zhang, Xin Zhang, Jiaming Liao, Linting Xu, Shaozhu Kang, Hong Chen, Mengshi Sun, Siting Wu, Zhuqing Xu, Shina Wei, Qiwei Qin, Jingguang Wei
Summary: This study cloned and identified a cGAS homolog, EccGAS, from orange-spotted grouper. EccGAS was found to be abundantly expressed in the blood, skin, and gills, and it inhibited the production of type I interferon and interacted with other signaling molecules, suggesting it may serve as a negative regulator of the cGAS-STING signaling pathway in fish.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Juliane Brun, Snezana Vasiljevic, Bevin Gangadharan, Mario Hensen, Anu Chandran, Michelle L. Hill, J. L. Kiappes, Raymond A. Dwek, Dominic S. Alonzi, Weston B. Struwe, Nicole Zitzmann
Summary: The study demonstrates the differences in site-specific glycosylation between virus-derived spikes, wild-type non-stabilized spikes, and recombinant engineered spike glycoproteins. Additionally, it shows that their unique cellular secretion pathways lead to distinct protein glycosylation and secretion patterns, including shedding of spike monomeric subunits for the non-stabilized wild-type spike, potentially impacting the resulting immune response and vaccine design.
ACS CENTRAL SCIENCE
(2021)
Article
Virology
Mohamed S. H. Hassan, Ahmed Ali, Motamed Elsayed Mahmoud, Danah Altakrouni, Shahnas M. Najimudeen, Mohamed Faizal Abdul-Careem
Summary: This study compared the efficacy of different vaccination programs against Canadian DMV/1639 IBV and found that the autogenous inactivated DMV/1639 vaccine was more effective in reducing viral loads and boosting virus neutralization titers. Both vaccination programs provided significant protection against pathological effects.
Article
Virology
Wanda Christ, Jonas Klingstrom, Janne Tynell
Summary: The integrated stress response (ISR) is activated in response to SARS-CoV-2 infection, triggering translational arrest and inhibiting stress granule formation. However, the stress-responsive transcription factors ATF4 and CHOP are not induced. Different SARS-CoV-2 variants show variant-specific differences in ISR activation, suggesting potential impacts on pathogenesis and treatment strategies.
Article
Virology
Xiao Han, Hankun Xu, Yifan Weng, Rong Chen, Jidong Xu, Tong Cao, Renjie Sun, Ying Shan, Fang He, Weihuan Fang, Xiaoliang Li
Summary: Classical swine fever virus (CSFV) can inhibit the host innate immunity by decreasing the level of high mobility group box 1 (HMGB1), and CSFV Npro can evade host antiviral immune response through upregulating HMGB1 acetylation.
Article
Virology
Sunjian Lyu, Fulei Xiong, Tianpeng Qi, Weifeng Shen, Qi Guo, Mingming Han, Li Liu, Weishao Bu, Julin Yuan, Bao Lou
Summary: This study identified and characterized a novel temperate A. hydrophila phage, P05B, which has strong lytic ability and stability across different temperatures and pH values.
Article
Virology
Siyuan Liu, Wei Chen, Raphael Nyaruaba, Shunlong Wang, Cihan Yang, Qun Wu, Ying Liu, Puyu Liu, Fei Wang, Jingling Wang, Zhiming Yuan, Dingwei Sun, Han Xia
Summary: Specific, sensitive, and reliable RT-qPCR assays for the detection of OYAV and EBIV have been successfully developed in this study. These assays hold great promise for their potential application in clinical and field samples in the future.
Article
Virology
Rui Wang, Qiang Sun, Jinbo Xiao, Congcong Wang, Xiaoliang Li, Jichen Li, Yang Song, Huanhuan Lu, Ying Liu, Shuangli Zhu, Zhijun Liu, Yong Zhang
Summary: Coxsackievirus A6 (CVA6) has emerged as an important agent causing hand, foot, and mouth disease (HFMD). Mutations in residue 64 of the virus have been found to increase resistance to ribavirin and decrease sensitivity to guanidine hydrochloride. This mutation also leads to increased replication fidelity and reduced pathogenicity. These findings provide a foundation for the development of a safe and effective live attenuated CVA6 vaccine.
Article
Virology
Yao Xiong, Keran Ma, Xiao Zou, Yantao Liang, Kaiyang Zheng, Tiancong Wang, Hong Zhang, Yue Dong, Ziyue Wang, Yundan Liu, Hongbing Shao, Andrew Mcminn, Min Wang
Summary: This study isolated a novel virus, vB_VviC_ZQ26, infecting Vibrio cyclitrophicus from coastal waters near Qingdao, China. It was found that vB_VviC_ZQ26 belongs to a new vibriophage-specific family, Coheviridae, and is mainly found in temperate and tropical epipelagic zones.
Article
Virology
Xing Li, Zhiping Ye, Ewan P. Plant
Summary: By analyzing clinical and cultured samples using the same bioinformatic pipeline, it was found that 5' copyback DVGs are prevalent in human clinical samples but not in cultured samples. Furthermore, there are differences in DVG production and composition between in vivo and in vitro infections.
Article
Virology
Shreya Banerjee, Rakesh Sarkar, Arpita Mukherjee, Suvrotoa Mitra, Animesh Gope, Mamta Chawla-Sarkar
Summary: Rotavirus is a major cause of viral gastroenteritis in infants and continues to be a severe issue in underdeveloped nations. Recent research has shown that a long non-coding RNA called SLC7A11-AS1 is upregulated during rotavirus infection and plays a role in facilitating the virus's propagation by regulating intracellular glutathione and lipid peroxidation.
Article
Virology
Eiichi Hondo, Tetsufumi Katta, Ayato Sato, Naoya Kadofusa, Tomoki Ishibashi, Hiroshi Shimoda, Hirokazu Katoh, Atsuo Iida
Summary: Bat-borne emerging viruses, such as the Ebola virus and Nipah virus, pose a significant threat to public health. In this study, we identified micafungin as a potential antiviral drug against Pteropine orthoreovirus (PRV) and found that it inhibits viral release in human cells through activating the endoplasmic reticulum stress response pathway. This research provides valuable insights into the development of effective treatments for future virus pandemics.
Article
Virology
M. Joubert, N. van den Berg, J. Theron, V. Swart
Summary: This study investigated the host gene expression in asymptomatic avocado nursery trees infected with Avocado sunblotch viroid (ASBVd) using RNA sequencing. The results showed that 631 genes were differentially expressed during infection, with 63% upregulated. ASBVd infection significantly affected plant defence responses, phytohormone networks, gene expression pathways, secondary metabolism, cellular transport, as well as protein modification and degradation. This study provides insights into the molecular interactions underlying avsunviroid-host interactions.
Article
Virology
Wanling Zhang, Haiyan Yang, Zhengyun Liu, Shengyu Wang, Tianyang Chen, Hong Song, Yunbin Xu, Fajin Li, Guo Luo, Huan Wang
Summary: EV71 infection leads to changes in mitochondrial morphology and dynamics in human neuroblastoma cells, resulting in mitochondrial dysfunction that may contribute to nervous system dysfunction.
Article
Virology
Mikhail Oliveira Leastro, Elliot Watanabe Kitajima, Vicente Pallas, Jesus Angel Sanchez- Navarro
Summary: This study reports the construction and validation of an infectious cDNA clone of Citrus leprosis virus C (CiLV-C) based on an agroinfection system. Agro-injected Nicotiana benthamiana plants showed localized lesions similar to the natural infection process. The virus recovered from the infected plant tissue could be mechanically transmitted between plants. The CiLV-C reverse genetic system provides a powerful molecular tool for unraveling the peculiarities of the citrus leprosis pathosystem.
Article
Virology
Manel Essaidi-Laziosi, Francisco J. Perez-Rodriguez, Catia Alvarez, Pascale Sattonnet-Roche, Giulia Torriani, Meriem Bekliz, Kenneth Adea, Matthias Lenk, Tasnim Suliman, Wolfgang Preiser, Marcel A. Mueller, Christian Drosten, Laurent Kaiser, Isabella Eckerle
Summary: This study found that the Omicron variant has different replication characteristics compared to other variants of concern, being able to efficiently produce infectious virus in nasal cells but not lung cells. In addition, the Delta and Omicron variants showed increased infection efficiency in A549 cells. These findings suggest that the variants of concern have better adaptation to humans but do not have an extended host range.
Article
Virology
Martin Faye, Modeste Name Faye, Babacar Ndiaye, Moussa Moise Diagne, Safietou Sankhe, Ndeye Marieme Top, Amadou Diallo, Cheikh Loucoubar, Ndongo Dia, Amadou Alpha Sall, Ousmane Faye
Summary: Genomic surveillance in Senegal since March 2020 has detected the emergence of Omicron BA.4 and BA.5 sublineages in June 2022. Next-generation sequencing and phylogeny analysis of isolates from a cluster of cases in Northern Senegal in July 2022 revealed that the BA.4 cases originated from a XAS recombinant, marking the first reported sequence of this variant from Senegal. Continuous genomic surveillance of positive SARS-CoV-2 samples is crucial.