期刊
VIRUS RESEARCH
卷 145, 期 1, 页码 97-105出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.virusres.2009.06.014
关键词
Porcine reproductive and respiratory; syndrome virus (PRRSV); Nsp2; ORF5; ORF7; Molecular variation and evolution
类别
资金
- National Natural Science Funds for Distinguished Young Scholar, National Natural Science Foundation of China [30825031]
- National Key Technology R&D Program of China [2006BAD06A03]
- National Key Basic Research Plan Grant, Chinese Ministry of Science and Technology [2005CB523204]
- University of China [IRT0866]
Porcine reproductive and respiratory syndrome virus (PRRSV) is characteristic of genetically extensive variation. The objective of the present study was to analyze the molecular variation and evolution of porcine reproductive and respiratory syndrome virus in China based on the complete genomic sequences of three highly pathogenic Chinese PRRSV strains isolated in 2006 and the sequences of the amplified Nsp2, ORF5 and CRF7 genes from clinical specimens during 2006-2008. Full-length genome sequencing and phylogenetic analysis showed that the three strains (JXwn06, Bjsy06 and NX06) had a unique 30-amino-acid discontinuous deletion in Nsp2, and were classified into the same subgroup that consisted of the most Chinese strains isolated during 2006-2007, the pandemic period of atypical PRRS. The evolution analysis suggested that the emergence of the highly pathogenic PRRSV in China experienced a gradual variation and evolution accumulation progress from Chinese domestic virus. The variation analysis of the amplified 41 Nsp2, 59 ORF5 and 59 ORF7 genes indicated that the diversity of PRRSV strain existed in the field, and the highly pathogenic PRRSV strain with the 30-amino-acid deletion in Nsp2 was the dominating virus in China in recent years. Our data contribute to the understanding of molecular variation and epidemiology surveillance of PRRSV in China. (C) 2009 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据