期刊
VIROLOGY JOURNAL
卷 11, 期 -, 页码 -出版社
BIOMED CENTRAL LTD
DOI: 10.1186/1743-422X-11-6
关键词
Serratia marcescens; Phage evolution; Genome; Proteome; Bioinformatics; Lysogeny; Unstable lysogeny; Modified nucleosides; Siphoviridae
类别
资金
- Natural Sciences and Engineering Research Council of Canada
Background: Serratia marcescens phage eta is a temperate unclassified member of the Siphoviridae which had been reported as containing hypermodified guanine residues. Methods: The DNA was characterized by enzymatic digestion followed by HPLC analysis of the nucleoside composition, and by DNA sequencing and proteomic analysis. Its ability to form stable lysogens and integrate was also investigated. Results: Enzymatic digestion and HPLC analysis revealed phage eta DNA did not contain modified bases. The genome sequence of this virus, determined using pyrosequencing, is 42,724 nucleotides in length with a mol% GC of 49.9 and is circularly permuted. Sixty-nine putative CDSs were identified of which 19 encode novel proteins. While seven close genetic relatives were identified, they shared sequence similarity with only genes 40 to 69 of the phage eta genome, while gp1 to gp39 shared no conserved relationship. The structural proteome, determined by SDS-PAGE and mass spectrometry, revealed seven unique proteins. This phage forms very unstable lysogens with its host S. marcescens.
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