Article
Environmental Sciences
Ju Mu, Yu Wang, Miao Wang, Dayi Zhang, Mingying Liu
Summary: This study constructed a mouse model exposed to poly-propylene microplastics (PP-MPs) to evaluate the bioaccumulation of PP-MPs in the kidney and explore potential reference genes using RT-qPCR. The stability and reliability of 19 candidate reference genes were assessed using four algorithms, and the top five most stable genes were determined (Cox4i1, Rps3, Rps9, Rps18, and Gapdh). Three genes associated with different biochemical processes were chosen to verify the identified reference genes, showing different expression profiles and emphasizing the importance of selecting reliable reference genes.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Multidisciplinary Sciences
Jianfeng Ma, Jingyun Chen, Mailin Gan, Lei Chen, Ye Zhao, Lili Niu, Yan Zhu, Shunhua Zhang, Xuewei Li, Zongyi Guo, Jinyong Wang, Li Zhu, Linyuan Shen
Summary: This study aimed to evaluate the expression stability of commonly used reference genes in mouse skeletal muscle tissue. ACTB, HPRT, and YWHAZ were identified as the most stable reference genes for gene expression normalization in murine skeletal muscle experiments.
Article
Plant Sciences
Maria Noelia Ulrich, Esteban Muniz-Padilla, Alejandra Corach, Esteban Hopp, Daniela Tosto
Summary: This study evaluated the stability of different reference genes of glyphosate-resistant S. halepense, finding that PP2A and ARI8 were the most stable genes. The results provide valuable information for gene expression analysis under glyphosate stress and will facilitate resistance mechanism studies in this weed species.
Article
Agriculture, Dairy & Animal Science
Maise dos Santos Macario, C. S. Nascimento, F. C. B. Sousa, I. R. S. Oliveira, A. P. D. Vesco, L. T. Barbosa, K. R. S. Sousa
Summary: This study evaluated the stability of quantitative real-time PCR in different tissues from male and female broiler quails and recommended suitable reference genes. Different tissues, including heart, thigh muscle, brain, and spleen, showed varying stability of reference genes.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Plant Sciences
Qizhang Wang, Chunqian Guo, Shipeng Yang, Qiwen Zhong, Jie Tian
Summary: The aim of this study was to identify suitable reference genes for gene expression analysis during different growth conditions in garlic. Nine candidate reference genes were selected using garlic transcriptome sequence data and their expression levels were evaluated in specific tissues under drought and cold stress. Several statistical methods were used to assess the stability of the reference gene expression levels. The most stable reference genes were identified, and their reliability was confirmed by evaluating the expression of a stress-responsive gene. This study provides a theoretical reference for gene expression analysis in garlic under stress conditions.
Article
Plant Sciences
Leandro Francisco de Oliveira, Amanda Rusiska Piovezani, Dimitre A. Ivanov, Leonardo Yoshida, Eny Iochevet Segal Floh, Massuo Jorge Kato
Summary: This study identified stable reference genes for gene expression analysis in four Piper species and provided a list of recommended gene pairs for each species. These findings will facilitate further transcriptomic and functional gene research.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Chaofan Jiang, Lin Zhou, Qingchen Zhao, Mengke Wang, Sirui Shen, Te Zhao, Kaidi Cui, Leiming He
Summary: This study validated the expression stability of seven candidate reference genes in Sclerotium rolfsii under different conditions and recommended suitable reference gene combinations for RT-qPCR normalization. The results showed that using one or two reference genes to normalize the expression of target genes in S. rolfsii did not significantly differ.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Jianfei Chen, Ziwei Chen, Jilong Zhang, Wuchao Zhuang, Xiaotong Zheng
Summary: This study utilized different algorithms to evaluate the stability of internal reference genes in the gastrointestinal tract of chickens. The most suitable reference genes for each type of GI tissue were identified. Commonly used internal reference genes exhibited poor stability and are not recommended for future gene expression studies in the GI region.
Article
Genetics & Heredity
Linlin Du, Bo Zeng, Xuejuan Li, Ying Lan, Wei Guo, Zhaoyun Wang, Zhiyang Liu, Yijun Zhou, Kumar Kunda Jiban, Tong Zhou
Summary: Accurate phenotypic identification is crucial for resistance genetics and rice breeding for RBSDV disease. A more efficient method of obtaining RBSDV-carrying SBPH population was improved based on the quantification of RBSDV copies, feeding duration on infected plants, and leaf stage of rice seedlings. The optimal conditions were found to be feeding on rice plants with RBSDV S10 copy numbers above 1.1*10(4) and transferring the insects to healthy rice seedlings after 7 or 9 days of feeding for 5 or 3 days. The 2-3-leaf stage was the best for inoculating rice seedlings, but older seedlings may require a higher rate of viruliferous SBPHs for inoculation.
FRONTIERS IN GENETICS
(2023)
Article
Fisheries
Joanna Szczygiel, Teresa Kaminska-Gibas, Jules Petit, Patrycja Jurecka, Geert Wiegertjes, Ilgiz Irnazarow
Summary: The study ranked the expression stability of duplicated genes in different tissues of common carp and found that 40s, b2m, ef1 alpha, and actb genes showed the most stable expression across all physiological conditions, while gapdh, odc1, and g6pd genes demonstrated lower stability. Functional divergence between duplicated genes was observed, emphasizing the importance of considering this aspect in selecting reference genes in polyploid species.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Genetics & Heredity
Dongxiao Ma, Qin Zhang, Jintao Zhou, Yu Lu, Xiaomeng Duan, Chengzhong He, Jinde Yu
Summary: In this study, the expression stability of 10 candidate reference genes was analyzed in Toxicodendron succedaneum using RT-qPCR. The results showed that TsRG03 and ACT were recommended as suitable reference genes for all samples. These findings provide alternative reference genes for gene function studies, correction and normalization of target gene expression, and directed molecular breeding in T. succedaneum.
Article
Biochemistry & Molecular Biology
Alice Nevone, Francesca Lattarulo, Monica Russo, Giada Panno, Paolo Milani, Marco Basset, Maria Antonietta Avanzini, Giampaolo Merlini, Giovanni Palladini, Mario Nuvolone
Summary: In the next-generation sequencing era, RT-qPCR remains widely used for quantifying nucleic acids due to its popularity, versatility, and cost-effectiveness. However, accurate measurement of transcriptional levels requires appropriate reference genes for normalization. Here, we present a strategy that utilizes publicly available transcriptomic datasets to select suitable reference genes for specific clinical or experimental settings and includes a pipeline for RT-qPCR assay design and validation. We demonstrated the efficacy of this strategy by identifying and validating reference genes for transcriptional studies of bone-marrow plasma cells in AL amyloidosis patients. This strategy can be applied to other settings with publicly available transcriptomic datasets.
Article
Plant Sciences
Francisca Valenzuela, Vivian D'Afonseca, Ricardo Hernandez, Aleydis Gomez, Ariel D. Arencibia
Summary: This study validates the reference genes in blueberry plants cultured in vitro on a colchicine-supplemented medium for the first time. The results show that colchicine treatments can cause genomic changes in blueberry plants and affect gene expression at the molecular level. The study also identifies potential reference genes for gene expression assays in Vaccinium.
Article
Genetics & Heredity
Yanli Liu, Chengcheng Zhang, Nunung Harijati, Ying Diao, Erxi Liu, Zhongli Hu
Summary: In this study, eight candidate internal reference genes were screened and detected using the Konjac transcriptome database of our laboratory and common internal reference genes in plants. They include 25S ribosomal RNA gene, 18S ribosomal RNA gene, actin gene, glyceraldehyde-3-phosphate dehydrogenase gene, ubiquitin gene, beta-tubulin gene, eukaryotic elongation factor 1-alpha gene, and eukaryotic translation initiation factor 4 beta-1 gene. The results from GeNorm, Normfinder, and BestKeeper were comprehensively analyzed. Stable expression levels were observed for 25S rRNA, 18S rRNA, and ACT during reproductive periods, eEF-1 alpha and eIF-4 alpha during nutritional periods, and eEF-1 alpha, UBQ, and ACT during different leaf developmental periods. These identified and stable internal reference genes will serve as a basis for future molecular biology-related studies of Konjac.
Article
Plant Sciences
Gang Li, Jiawei Ma, Junliang Yin, Fengling Guo, Keyong Xi, Peihua Yang, Xiaodong Cai, Qie Jia, Lu Li, Yiqing Liu, Yongxing Zhu
Summary: This study identified appropriate reference genes for RT-qPCR in ginger and facilitated gene expression studies under biotic and abiotic stress conditions.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Virology
Gabriela Llauger, Luis Alejandro de Haroa, Victoria Alfonso, Mariana del Vas
Article
Plant Sciences
Luis A. de Haro, Analia D. Dumon, Maria F. Mattio, Evangelina Beatriz Arguello Caro, Gabriela Llauger, Diego Zavallo, Herve Blanc, Vanesa C. Mongelli, Graciela Truol, Maria-Carla Saleh, Sebastian Asurmendi, Mariana del Vas
FRONTIERS IN PLANT SCIENCE
(2017)
Article
Entomology
Evangelina B. Argueello Caro, Guillermo A. Maroniche, Analia D. Dumon, Monica B. Sagadin, Mariana Del Vas, Graciela Truol
ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA
(2013)
Article
Biochemistry & Molecular Biology
G. A. Maroniche, V. C. Mongelli, V. Alfonso, G. Llauger, O. Taboga, M. del Vas
INSECT MOLECULAR BIOLOGY
(2011)
Article
Biotechnology & Applied Microbiology
E. Bossio, A. Diaz Paleo, M. del Vas, I. Baroli, A. Acevedo, R. D. Rios
PLANT CELL TISSUE AND ORGAN CULTURE
(2013)
Article
Multidisciplinary Sciences
Victoria Alfonso, Guillermo A. Maroniche, Sol R. Reca, Maria Gabriela Lopez, Mariana del Vas, Oscar Taboga
Article
Plant Sciences
Analia D. Dumon, Evangelina B. Argueello-Caro, Maria V. Alemandri, Carlos Bainotti, Maria F. Mattio, Sandra M. Rodriguez, Mariana del Vas, Graciela Truol
TROPICAL PLANT PATHOLOGY
(2011)
Article
Virology
Guillermo A. Maroniche, Vanesa C. Mongelli, Gabriela Llauger, Victoria Alfonso, Oscar Taboga, Mariana del Vas
Article
Plant Sciences
Luis Alejandro de Haro, Sofia Maite Arellano, Ondrej Novak, Regina Feil, Analia Delina Dumon, Maria Fernanda Mattio, Danuse Tarkowska, Gabriela Llauger, Miroslav Strnad, John Edward Lunn, Stephen Pearce, Carlos Maria Figueroa, Mariana del Vas
Article
Biotechnology & Applied Microbiology
M. Agostina Affinito, Fabiana D. Espasandin, Mariana del Vas, Mayra Y. Alvarez, Adriana N. Andres, Pedro A. Sansberro, Antonio H. Diaz Paleo
Summary: Lotus tenuis, a perennial forage legume in Argentina, utilizes NHX antiporters for compartmentalizing Na+ into vacuoles to reduce ion toxicity during salt stress. The vacuolar localization activity of LtNHX1 antiporter contributes to salt tolerance in Lotus tenuis.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Biotechnology & Applied Microbiology
Juan M. Crescente, Diego Zavallo, Mariana del Vas, Sebastian Asurmendi, Marcelo Helguera, Elmer Fernandez, Leonardo S. Vanzetti
Summary: This study analyzed small RNA and degradome libraries from Triticum aestivum and found that MITEs (Miniature Inverted-repeat Transposable Elements) may be the origin of some miRNAs, and miRNAs derived from MITEs preferentially target degradation sites with MITE insertions in the 3' UTR regions of transcripts. This regulatory network could have a significant impact on the post-transcriptional control of gene expression.
Article
Multidisciplinary Sciences
Gabriela Llauger, Demian Monti, Matias Aduriz, Ema Romao, Analia Delina Dumon, Maria Fernanda Mattio, Andres Wigdorovitz, Serge Muyldermans, Cecile Vincke, Viviana Parreno, Mariana del Vas
Summary: The study focused on isolating and characterizing llama-derived Nanobodies (Nbs) that recognize the major viral viroplasm component, P9-1. These Nbs were successfully employed to detect P9-1 in infected maize extracts and to immunodetect virus present in phloem cells of leaf thin sections. The development of these Nbs will contribute to the study of MRCV epidemiology and fundamental research on viroplasm structure and maturation.
SCIENTIFIC REPORTS
(2021)
Article
Virology
Fernanda Maurino, Analia D. Dumon, Gabriela Llauger, Vanina Alemandri, Luis A. de Haro, M. Fernanda Mattio, Mariana del Vas, Irma Graciela Laguna, Maria de la Paz Gimenez Pecci
ARCHIVES OF VIROLOGY
(2018)
Article
Entomology
A. D. Dumon, E. B. Arguello Caro, M. F. Mattio, V. Alemandri, M. del Vas, G. Truol
BULLETIN OF ENTOMOLOGICAL RESEARCH
(2018)