Article
Microbiology
Gerardo Ramirez-Rico, Moises Martinez-Castillo, Roberto Cardenas-Zuniga, Daniel Coronado-Velazquez, Angelica Silva-Olivares, Mireya De la Garza, Mineko Shibayama, Jesus Serrano-Luna
Summary: In this study, the resistance of A. castellanii to bovine apo-lactoferrin was investigated. It was found that high concentrations of apo-lactoferrin could effectively inhibit the cytopathic effect of A. castellanii on MDCK cells.
Article
Biochemistry & Molecular Biology
Reka Fanni Barna, Mate Mackei, Erzsebet Paszti-Gere, Zsuzsanna Neogrady, Akos Jerzsele, Gabor Matis
Summary: This study investigated the effects of matriptase inhibitors MI432 and MI460 on the inflammatory and oxidative state of chicken hepatocytes, finding that while the inhibitors temporarily reduced metabolic activity, they did not significantly affect oxidative stress and lipid peroxidation. These results suggest that matriptase activities may play a key role in maintaining metabolic and inflammatory homeostasis in chicken livers.
Article
Immunology
Lauren A. Chang, Angela Choi, Raveen Rathnasinghe, Prajakta Warang, Moataz Noureddine, Sonia Jangra, Yong Chen, Bruno G. De Geest, Michael Schotsaert
Summary: Eosinophils play important roles in mucosal tissue homeostasis, anti-helminth responses, and allergy. Lung eosinophilia is associated with abnormal T cell responses to respiratory viral infections and vaccine-associated enhanced respiratory disease (VAERD). Our study found that vaccinated mice showed increased influx of eosinophils in the lungs after a vaccine-matched sublethal influenza infection.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Seyed Mohammad Ghafoori, Gayle F. Petersen, Deborah G. Conrady, Brandy M. Calhoun, Matthew Z. Z. Stigliano, Ruth O. Baydo, Rena Grice, Jan Abendroth, Donald D. Lorimer, Thomas E. Edwards, Jade K. Forwood
Summary: Influenza virus causes annual outbreaks of flu, resulting in a significant economic burden on the healthcare system. It has caused several pandemics with millions of deaths in the past century. This study characterizes the structures of seven influenza A virus hemagglutinins (HAs) and identifies conserved residues involved in receptor binding. The study also shows that modifications in a certain region disrupt the binding of a specific antibody, providing new insights for anti-influenza vaccine development.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Multidisciplinary
Stefan Woerner, Bernhard N. Bohnert, Matthias Woern, Mengyun Xiao, Andrea Janessa, Andreas L. Birkenfeld, Kerstin Amann, Christoph Daniel, Ferruh Artunc
Summary: High doses of aprotinin exert nephrotoxic effects in healthy mice by accumulating in the tubular system, inhibiting proximal tubular function, and counterregulatory stimulation of ENaC-mediated sodium transport. Under a low-salt diet, aprotinin impaired sodium preservation, stimulated excessive hyperaldosteronism, and unexpectedly, activated ENaC.
ACTA PHARMACOLOGICA SINICA
(2022)
Article
Pharmacology & Pharmacy
Yuling Tang, Ruijing Su, Qingyue Gu, Yanxin Hu, Hanchun Yang
Summary: Influenza A virus infection leads to significant morbidity and mortality worldwide, and secondary bacterial infection worsens the severity and fatality of the initial viral infection. The role of mast cells in viral and bacterial co-infection is not well understood.
ANTIVIRAL RESEARCH
(2023)
Article
Cell Biology
Aicha Kriaa, Amin Jablaoui, Soufien Rhimi, Souha Soussou, Hela Mkaouar, Vincent Mariaule, Natalia Gruba, Ali Gargouri, Emmanuelle Maguin, Adam Lesner, Moez Rhimi
Summary: The study found that gut microbial protease activity may be an important contributor to IBD, and oral administration of recombinant bacteria secreting SP-1 compromises the epithelial barrier, alters the microbial community, and exacerbates colitis. This suggests that targeting gut microbial protease activity could be a promising approach for treating the disease.
Article
Chemistry, Applied
Ester Goncalves de Jesus, Fernanda Fernandes de Souza, Joao Victor Andrade, Marcio Luis Andrade e Silva, Wilson R. Cunha, Rafael Correa Ramos, Othon Souto Campos, Jorge Alexandre Nogueira Santos, Mario F. C. Santos
Summary: The inhibition potential of Elastase from the porcine pancreas by rosmarinic acid, a compound commonly found in Rosemary plants, was evaluated in silico and in vitro. Molecular docking was used to investigate the mechanism of action, and it was found that rosmarinic acid significantly inhibited Elastase in a range of 5-60 mu g/mL, with 55% inhibition at 60 mu g/mL. These results suggest that rosmarinic acid can be used as a basis for developing new enzyme inhibitors for various drugs, including anticancer drugs.
NATURAL PRODUCT RESEARCH
(2023)
Article
Medicine, Research & Experimental
Kurt Brassington, Stanley M. H. Chan, Simone N. De Luca, Aleksandar Dobric, Suleman A. Almerdasi, Kevin Mou, Huei Jiunn Seow, Osezua Oseghale, Steven Bozinovski, Stavros Selemidis, Ross Vlahos
Summary: People with COPD are at risk of respiratory infections, which can lead to death. This study found that chronic cigarette smoke exposure worsened vascular function and influenza A virus infection further exacerbated the dysfunction. However, treatment with the antioxidant ebselen alleviated the vascular dysfunction and reduced lung inflammation caused by smoke exposure and flu infection.
Article
Immunology
Faris Almutairi, Demba Sarr, Samantha L. Tucker, Kayla Fantone, Jae-Kyung Lee, Balazs Rada
Summary: RGS10 plays a crucial role in modulating lung immune and inflammatory responses to influenza virus infection, with deficiency leading to more severe disease progression and faster onset. This suggests RGS10 as a potential therapeutic target for respiratory infections.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Microbiology
Yulia Desheva, Galina Leontieva, Tatiana Kramskaya, Igor Losev, Andrey Rekstin, Nadezhda Petkova, Polina Kudar, Alexander Suvorov
Summary: This study examines the resistance of mice against early influenza infection and post-influenza pneumococcal pneumonia after intranasal live influenza vaccine (LAIV) administration. The results show that LAIV provides early protection against both homologous and heterologous viral infections and has a protective effect against post-influenza pneumococcal infection. These findings suggest that intranasal administration of LAIV may be valuable in preventing not only influenza, but also other acute respiratory infections.
Article
Parasitology
Hao-Chengip Wang, Qiu-Hui Wang, Biswajit Bhowmick, Yi-Xun Li, Qian Han
Summary: The study investigated the functional characterization of Ae-CLIPB15 and Ae-CLIPB22 in Ae. aegypti, revealing their critical roles in immune defense against bacterial and fungal infections through regulation of transcription factors and PO activity. These findings suggest that Ae-CLIPB15 and Ae-CLIPB22 play a specific role in the regulation of innate immunity.
PARASITES & VECTORS
(2021)
Article
Cell Biology
Jaekeun Park, Sharon L. Fong Legaspi, Louis M. Schwartzman, Sebastian M. Gygli, Zhong-Mei Sheng, Ashley D. Freeman, Lex M. Matthews, Yongli Xiao, Mitchell D. Ramuta, Natalia A. Batchenkova, Li Qi, Luz Angela Rosas, Stephanie L. Williams, Kelsey Scherler, Monica Gouzoulis, Ian Bellayr, David M. Morens, Kathie-Anne Walters, Matthew J. Memoli, John C. Kash, Jeffery K. Taubenberger
Summary: A new type of influenza vaccine has demonstrated broad protection against multiple IAV subtypes in both mice and ferrets. This vaccine approach shows the feasibility of eliciting broad, heterosubtypic IAV protection and identifies a promising candidate for influenza vaccine clinical development.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Nutrition & Dietetics
Weimin Guo, Dayong Wu, Lijun Li, Samuel Ding, Simin Nikbin Meydani
Summary: Obesity may contribute to impaired immune function and increased susceptibility to infection.
FRONTIERS IN NUTRITION
(2022)
Article
Cell Biology
Melanie Lavaud, Mathilde Mullard, Robel Tesfaye, Jerome Amiaud, Melanie Legrand, Geoffroy Danieau, Regis Brion, Sarah Morice, Laura Regnier, Maryne Dupuy, Benedicte Brounais-Le Royer, Francois Lamoureux, Benjamin Ory, Francoise Redini, Franck Verrecchia
Summary: Osteosarcoma is the most common malignant bone tumor in children and teenagers, with low survival rates in patients with poor treatment response or metastases. Studies have shown increased expression of four USPs in OS tumor cells, with USP6 and USP41 expression correlated with patient survival. In vivo experiments with a USP inhibitor demonstrated reduced tumor growth and metastases in a preclinical OS model, while in vitro experiments showed decreased cell viability in OS cells through apoptosis induction.
Article
Biotechnology & Applied Microbiology
Ruth L. O. Lambertz, Ingo Gerhauser, Inga Nehimeier, Sarah R. Leist, Heike Kollmus, Stefan Poehlmann, Klaus Schughart
JOURNAL OF GENERAL VIROLOGY
(2019)
Article
Virology
Ruth Lydia Olga Lambertz, Ingo Gerhauser, Inga Nehlmeier, Sabine Gaertner, Michael Winkler, Sarah Rebecca Leist, Heike Kollmus, Stefan Poehlmann, Klaus Schughart
Article
Biochemistry & Molecular Biology
Tarek Kamal Motawi, Nancy Nabil Shahin, Kareem Awad, Amany Sayed Maghraby, Dina Nadeem Abd-Elshafy, Mahmoud Mohamed Bahgat
Article
Virology
Mahmoud M. Bahgat, Rola Nadeem, Mohamed H. Nasraa, Mona A. -E. Awad, Solaf Kamel, Dina N. Abd-Elshafy
Summary: The study found that socioeconomic level, gender, and age impact humoral responses to SARS-CoV-2, with differences in IgM and IgG prevalence between low and high socioeconomic groups. There is individual heterogeneity in responses to infection in terms of symptoms.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Correction
Multidisciplinary Sciences
Subhashini A. Sellers, William A. Fischer, Mark T. Heise, Klaus Schughart
SCIENTIFIC REPORTS
(2021)
Article
Respiratory System
Alexander Pausder, Jennifer Fricke, Klaus Schughart, Jens Schreiber, Till Strowig, Dunja Bruder, Julia D. Boehme
Summary: Respiratory Pigr expression and IgA levels are influenced by exposure to microbial stimuli, with differential effects of LPS and IFN-gamma on URT and LRT. Prophylactic IFN-gamma treatment can reduce nasal colonization with S. pneumoniae.
Article
Microbiology
Patrick Waindok, Elisabeth Janecek-Erfurth, Dimitri L. Lindenwald, Esther Wilk, Klaus Schughart, Robert Geffers, Christina Strube
Summary: This study investigated the gene expression changes in the brains of mice infected with Toxocara and identified dysregulated pathways associated with cholesterol biosynthesis, apoptosis signaling, and immune response. The findings provide insights into the pathogenesis of neurotoxocarosis and suggest a potential link between Toxocara infection and dementia or Alzheimer's disease.
Article
Environmental Sciences
Dina Nadeem Abd-ElShafy, Rola Nadeem, Mahmoud Mohamed Bahgat
Summary: This study aimed to test the inhibitory effect of Camellia sinensis (black tea) water extracts on respiratory viruses and the inhibition of viruses accumulated over different surface types. The results showed that black tea extracts in water had a good inhibitory effect on the viruses, effectively inhibiting the infection of influenza A virus and adenovirus.
JOURNAL OF WATER AND HEALTH
(2022)
Article
Biochemistry & Molecular Biology
Ya Wang, Klaus Schughart, Tiana Maria Pelaia, Tracy Chew, Karan Kim, Thomas Karvunidis, Ben Knippenberg, Sally Teoh, Amy L. Phu, Kirsty R. Short, Jonathan Iredell, Irani Thevarajan, Jennifer Audsley, Stephen Macdonald, Jonathon Burcham, Benjamin Tang, Anthony McLean, Maryam Shojaei
Summary: Compared to healthy individuals and patients with mild/moderate COVID-19, severe COVID-19 patients showed upregulation of growth factor and cell cycle signaling pathways, with downregulation of interferon signaling pathways. Matrix metallopeptidase 9 (MMP9) was found in five of the top 10 upregulated pathways, indicating its potential as a therapeutic target against COVID-19.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Dina Nadeem Abd-Elshafy, Rola Nadeem, Mahmoud Mohamed Bahgat
Summary: This study investigates the impact of people's hygienic habits on the prevalence of enteric viruses. The analysis of samples collected from a wastewater treatment plant in Egypt reveals a significant decrease in the prevalence rates of adenovirus, hepatitis A virus (HAV), and rotavirus after the COVID-19 pandemic.
JOURNAL OF WATER AND HEALTH
(2022)
Article
Multidisciplinary Sciences
Laura Heydemann, Malgorzata Ciurkiewicz, Georg Beythien, Kathrin Becker, Klaus Schughart, Stephanie Stanelle-Bertram, Berfin Schaumburg, Nancy Mounogou-Kouassi, Sebastian Beck, Martin Zickler, Mark Kuehnel, Guelsah Gabriel, Andreas Beineke, Wolfgang Baumgaertner, Federico Armando
Summary: This study investigates the morphology, phenotype, and transcriptomic features of alveolar regeneration in SARS-CoV-2 infected Syrian golden hamsters. The results demonstrate that the hamster model reliably phenocopies indicators of a dysregulated alveolar regeneration of COVID-19 patients. This provides important information for future research on the pathomechanisms of PASC and for testing prophylactic and therapeutic approaches.
NATURE COMMUNICATIONS
(2023)
Article
Immunology
Rola Nadeem, Amany Sayed Maghraby, Dina Nadeem Abd-Elshafy, Ahmed Barakat Barakat, Mahmoud Mohamed Bahgat
Summary: This study reports on an HCV DNA vaccine candidate based on selected envelope epitopes and evaluates its expression and processing in human PBMCs and the cellular response in mice. The results show inter-individual variation in antigen expression and reactivity levels to antibodies. However, this vaccine candidate may induce a promising natural immune response, potentially priming CD4+ T-cell response.
CLINICAL AND EXPERIMENTAL VACCINE RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Rola Nadeem, Ahmed B. Barakat, Mahmoud M. Bahgat
Summary: The study found that it is possible to use both Swiss albino mice and albino rats in a single sandwich ELISA, with the captured antibody species from the Swiss albino mice and the detection antibody from the albino rat. This study is the first to cover the optimal order of capturing and detection antibodies in a sandwich ELISA assay, addressing possible interfering cross-reactivity when using mouse and rat serum antibodies in a single assay.
JOURNAL OF GENETIC ENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biology
Velma Herwanto, Benjamin Tang, Ya Wang, Maryam Shojaei, Marek Nalos, Amith Shetty, Kevin Lai, Anthony S. McLean, Klaus Schughart
Summary: The data consists of transcriptome data obtained by RNA sequencing from patients with uncomplicated infections, sepsis, septic shock, and healthy controls. This data allows detailed analyses of gene expression changes, identification of differentially expressed genes, co-regulated modules, and pathway activation studies.
BMC RESEARCH NOTES
(2021)
Article
Medicine, General & Internal
Amany S. Maghraby, Solaf A. Kamel, Dalia B. Fayed, Heba Shawky, Mona A. Razin, Rola N. Abd-Elshafy, Azza F. Arafa, Dina N. Abd-Elshafy, Mahmoud M. Bahgat
RUSSIAN OPEN MEDICAL JOURNAL
(2019)