Article
Agriculture, Multidisciplinary
Moana Lima Tavares-Esashika, Ravi Narayan Souza Campos, Matheus Hideki Kihara Maeda, Lucas Hideo Hataka Koyama, Pedro Ricardo Vieira Hamann, Eliane Ferreira Noronha, Tatsuya Nagata
Summary: This study assessed the use of the infectious clone of pepper ringspot virus as a heterologous gene expression tool in Nicotiana benthamiana plants. The researchers successfully inserted the gene of interest and the reporter gene into the virus, resulting in high protein yield. The study demonstrates the efficient production of enzymatically active heterologous proteins using this plant system.
ANNALS OF APPLIED BIOLOGY
(2022)
Editorial Material
Plant Sciences
Erich Y. T. Nakasu, Tatsuya Nagata, Alice K. Inoue-Nagata
Article
Agriculture, Multidisciplinary
Danielle Caroline Manenti, Jackson Araujo, Matheus Hideki Kihara Maeda, Moana Lima Tavares-Esashika, Paulo Hugo Aguiar, Anderson Rotter Meda, Tatsuya Nagata, Eliezer Rodrigues de Souto
Summary: This study overcame the challenges in studying viral diseases in cotton plants by using new experimental techniques and genotyping protocols. The results suggest that atypical-CBD may dominate in cotton fields in Brazil.
ANNALS OF APPLIED BIOLOGY
(2023)
Article
Virology
Caterynne Melo Kauffmann, Alessandra de Jesus Boari, Joao Marcos Fagundes Silva, Rosana Blawid, Tatsuya Nagata
Summary: A cytorhabdovirus, tentatively named patchouli chlorosis-associated cytorhabdovirus (PCaCV), was identified in a patchouli plant using high-throughput sequencing. The genome sequence of PCaCV was confirmed and found to contain six open reading frames. PCaCV is most closely related to tomato yellow mottle-associated virus and should be considered a new member of the genus Cytorhabdovirus.
ARCHIVES OF VIROLOGY
(2022)
Editorial Material
Plant Sciences
R. Ramos-Sobrinho, M. M. M. Ferro, G. S. A. Lima, T. Nagata
Article
Virology
Josilene R. Pinheiro, Esther C. dos Reis, Jessica P. Farias, Mayanna M. C. Fogaca, Patricia de S. da Silva, Itana Vivian R. Santana, Ana Luiza S. Rocha, Paloma O. Vidal, Rafael da C. Simoes, Wilson B. Luiz, Alexander Birbrair, Renato S. de Aguiar, Renan P. de Souza, Vasco A. de C. Azevedo, Gepoliano Chaves, Aline Belmok, Ricardo Duraes-Carvalho, Fernando L. Melo, Bergmann M. Ribeiro, Jaime Henrique Amorim
Summary: This study investigated the epidemiological dynamics of COVID-19 in a Brazilian population of nearly 17,000 patients over a 15-month period. The study found that COVID-19 cases and deaths fluctuated over time, with men and individuals over the age of 60 being the most affected by deaths. Furthermore, individuals between the ages of 30 and 44 were the most affected by COVID-19 cases. The study also revealed that viral loads in the nasopharynx were higher during the early symptomatic period. Additionally, the study discovered a significant increase in deaths during the second half of the study period, as early pandemic SARS-CoV-2 lineages were replaced by the variant of concern (VOC) P.1 (Gamma).
Article
Virology
Ueric Jose Borges de Souza, Raissa Nunes dos Santos, Marta Giovanetti, Luiz Carlos Junior Alcantara, Jucimaria Dantas Galvao, Franciano Dias Pereira Cardoso, Feliph Cassio Sobrinho Brito, Ana Claudia Franco, Paulo Michel Roehe, Bergmann Morais Ribeiro, Fernando Rosado Spilki, Fabricio Souza Campos
Summary: This study investigates the genomic epidemiology of the CHIKV virus in Tocantins state, Brazil, and finds that the virus belongs to the ECSA lineage and is closely related to outbreaks in Northeast and Southeast Brazil. The study identifies several frequent non-synonymous mutations in the virus genome, indicating local transmission in the state. The findings highlight the importance of continued genomic surveillance for predicting new infections and improving public health services.
Article
Microbiology
Filipe Vieira Santos de Abreu, Kamila Lorene Soares Rocha, Ramon Silva-Oliveira, Mariana Viana Macedo, Thamires Gabriele Macedo Silva, Maria Eduarda Goncalves-Dos-Santos, Cirilo Henrique de Oliveira, Sandy Micaele Aquino-Teixeira, Vinicius de Oliveira Ottone, Alex Junio Jardim da Silva, Ronaldo Medeiros Dos Santos, Aline Tatila-Ferreira, Marco Antonio Barreto de Almeida, Edmilson Dos Santos, Jader da Cruz Cardoso, Aline Alves Scarpellini Campos, George Rego Albuquerque, Anaia da Paixao Seva, Bergmann Morais Ribeiro, Danilo Simonini Teixeira, Fabricio Souza Campos, Ana Claudia Franco, Paulo Michel Roehe, Giliane de Souza Trindade, Danilo Bretas de Oliveira
Summary: Evidence of Orthopoxvirus (OPXV) infection has been detected for the first time in neotropical free-living primates in southeast Brazil, indicating their previous exposure to the virus. This finding is significant for understanding the transmission and infection risks of OPXV in primates.
Article
Virology
Leonardo Assis da Silva, Marcos Fernando Basso, Bergmann Morais Ribeiro
Summary: The cotton boll weevil is a major pest of cotton, causing significant losses in yield and fiber quality. In this study, a new virus, named Anthonomus grandis iflavirus 1 (AgIV-1), was discovered in apparently healthy native adult cotton boll weevils using a metagenomic approach. The complete genome sequence of AgIV-1 was obtained, and it could potentially be used for developing control tools for cotton boll weevils.
ARCHIVES OF VIROLOGY
(2023)
Article
Virology
Caterynne Melo Kauffmann, Alessandra de Jesus Boari, Joao Marcos Fagundes Silva, Rosana Blawid, Tatsuya Nagata
Summary: A new sobemovirus, named mimosa mosaic virus (MimMV), was discovered and isolated from a mimosa plant using high-throughput sequencing. The genome sequence, confirmed by Sanger sequencing, consists of 4595 nucleotides. Phylogenetic analysis based on predicted amino acid sequences showed that MimMV shares 52.7% and 31.8% sequence identity with the P2b protein and coat protein of blueberry shoestring virus, respectively. The complete genome sequence of MimMV shares less than 47% identity with other sobemoviruses, suggesting that it belongs to a new species named Sobemovirus mimosae.
ARCHIVES OF VIROLOGY
(2023)
Correction
Virology
Monique M. van Oers, Elisabeth A. Herniou, Johannes A. Jehle, Peter J. Krell, Adly M. M. Abd-Alla, Bergmann M. Ribeiro, David A. Theilmann, Zhihong Hu, Robert L. Harrison
ARCHIVES OF VIROLOGY
(2023)
Article
Microbiology
Leonardo Lopes-Luz, Matheus Bernardes Torres Fogaca, Brenda Garcia Bentivoglio-Silva, Djairo Pastor Saavedra, Luana Michele Alves, Luisa Valerio Franca, Gildemar Jose Bezerra Crispim, Ikaro Alves de Andrade, Bergmann Morais Ribeiro, Tatsuya Nagata, Samira Buhrer-Sekula
Summary: The study introduces a novel and highly specific IgM antibody capture ELISA (MAC-ELISA) for early diagnosis of COVID-19, utilizing biotinylated recombinant SARS-CoV-2 nucleocapsid (N) antigen produced in plants. The assay showed the highest sensitivity of 81.2% in the 8-14 days post-symptom group, offering a streamlined and cost-effective alternative to molecular diagnostics.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2023)
Article
Plant Sciences
Macaria Ferreira Duarte, Ikaro Alves de Andrade, Joao Marcos Fagundes Silva, Fernando Lucas de Melo, Ana Maria Machado, Alice Kazuko Inoue-Nagata, Tatsuya Nagata
Summary: Frequent monitoring of emerging viruses of agricultural crops is essential for plant virologists. In this study, sewage water samples were used to monitor plant viruses using high-throughput sequencing (HTS) analysis and RT-qPCR. The study identified 12 virus families, including common and stable viruses, and also discovered a quarantine virus and a new tobamovirus species. The findings showed a high correlation between virus abundance in sewage and processed food sources, indicating the potential use of sewage for virus surveillance.
TROPICAL PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Caterynne M. Kauffmann, Alessandra J. Boari, Joao M. F. Silva, Rosana Blawid, Elliot W. Kitajima, Tatsuya Nagata
Summary: Black pepper virus F (BPVF), previously found only in China, was identified in black pepper plants in Brazil. The presence of BPVF was confirmed in 66.66% of black pepper plants in five municipalities in the State of Para.
TROPICAL PLANT PATHOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Marcela Guimaraes Landim, Marcella Lemos Brettas Carneiro, Graziella Anselmo Joanitti, Carla Tatiana Mota Anflor, David Dobkowski Marinho, Jose Filipe Bacalhau Rodrigues, Wladymyr Jefferson Bacalhau de Sousa, Daniel de Oliveira Fernandes, Beatriz Ferreira Souza, Alicia Simalie Ombredane, Jessica Catarine Frutuoso do Nascimento, Gisela de Jesus Felice, Aline Midori Adati Kubota, Juliana Simas Coutinho Barbosa, John Hideki Ohno, Solomon Kweku Sagoe Amoah, Lindomar Jose Pena, Glecia Virgolino da Silva Luz, Laise Rodrigues de Andrade, Willie Oliveira Pinheiro, Bergmann Morais Ribeiro, Fabio Rocha Formiga, Marcus Vinicius Lia Fook, Mario Fabricio Fleury Rosa, Henry Maia Peixoto, Rodrigo Luiz Carregaro, Suelia de Siqueira Rodrigues Fleury Rosa
Summary: The novel N95 respirator using chitosan nanoparticles has demonstrated enhanced mechanical, antimicrobial, and antiviral characteristics when compared to traditional N95 respirators. Tests have shown increased resistance to aerosol penetration, improved filtration of microbial and viral particles, and strong antimicrobial activity.