Letter
Pediatrics
Ivana Pennisi, Jesus Rodriguez-Manzano, Ahmad Moniri, Myrsini Kaforou, Jethro A. Herberg, Michael Levin, Pantelis Georgiou
Summary: This study evaluates a 2-gene RNA signature that can be transformed into a rapid and portable laboratory-on-a-chip platform for point-of-care testing, which can be completed in less than 25 minutes.
Article
Chemistry, Multidisciplinary
Jesus Rodriguez-Manzano, Kenny Malpartida-Cardenas, Nicolas Moser, Ivana Pennisi, Matthew Cavuto, Luca Miglietta, Ahmad Moniri, Rebecca Penn, Giovanni Satta, Paul Randell, Frances Davies, Frances Bolt, Wendy Barclay, Alison Holmes, Pantelis Georgiou
Summary: The study presents a rapid PoC diagnostic test based on RT-LAMP and semiconductor technology for detecting SARS-CoV-2 in less than 20 minutes. The test demonstrates high sensitivity and specificity in clinical samples, showing promise as a rapid detection tool for tackling the COVID-19 pandemic.
ACS CENTRAL SCIENCE
(2021)
Article
Engineering, Biomedical
Ahmad Moniri, Dan Terracina, Jesus Rodriguez-Manzano, Paul H. Strutton, Pantelis Georgiou
Summary: This study investigates the use of adaptive algorithms, specifically a deep convolutional neural network, to forecast sEMG features of trunk muscles. The CNN can accurately forecast 25 seconds ahead with good precision, outperforming shallow models and providing guidance for muscle fatigue prediction and therapy.
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
(2021)
Article
Engineering, Biomedical
George Alexandrou, Nicolas Moser, Katerina-Theresa Mantikas, Jesus Rodriguez-Manzano, Simak Ali, R. Charles Coombes, Jacqui Shaw, Pantelis Georgiou, Christopher Toumazou, Melpomeni Kalofonou
Summary: ESR1 mutations serve as important biomarkers for metastatic breast cancer, particularly in patients with HR+ breast cancer undergoing hormonal therapies. This study demonstrates the efficacy of a LoC system for detecting and differentiating WT and MT copies of the ESR1 gene, offering potential for use in breast cancer point-of-care testing and mutational tracking in liquid biopsies.
IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS
(2021)
Article
Food Science & Technology
Marta Itarte, Sandra Martinez-Puchol, Eva Fores, Ayalkibet Hundesa, Natalia Timoneda, Silvia Bofill-Mas, Rosina Girones, Marta Rusinol
Summary: Fresh fruits and vegetables are vulnerable to microbial contamination and irrigation water quality is crucial. The study found frequent viral contamination in fresh produce, particularly Norovirus and Human Papillomavirus. The presence of these viruses in both fresh produce and irrigation water implies that irrigation water might be the main source of viral pathogens in food samples.
Article
Infectious Diseases
Ho Kwong Li, Myrsini Kaforou, Jesus Rodriguez-Manzano, Samuel Channon-Wells, Ahmad Moniri, Dominic Habgood-Coote, Rishi K. Gupta, Ewurabena A. Mills, Dominique Arancon, Jessica Lin, Yueh-Ho Chiu, Ivana Pennisi, Luca Miglietta, Ravi Mehta, Nelofar Obaray, Jethro A. Herberg, Victoria J. Wright, Pantelis Georgiou, Laura J. Shallcross, Alexander J. Mentzer, Michael Levin, Graham S. Cooke, Mahdad Noursadeghi, Shiranee Sriskandan
Summary: A three-gene transcriptional signature has been identified in this study to effectively differentiate between bacterial and viral infections, including COVID-19. This signature demonstrates superior performance in discriminating viral infections, offering potential clinical utility in managing acute presentations with infection.
Article
Oncology
Benjamin Wormald, Jesus Rodriguez-Manzano, Nicolas Moser, Ivana Pennisi, Thomas E. J. Ind, Katherine Vroobel, Ayoma Attygalle, Pantelis Georgiou, Nandita M. deSouza
Summary: The study aimed to establish a sensitive and specific HPV and tumor marker DNA/mRNA assay for early detection of cervical cancer, and determine its diagnostic benefit when used in conjunction with high-resolution endovaginal magnetic resonance imaging (MRI).
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Luca Miglietta, Ahmad Moniri, Ivana Pennisi, Kenny Malpartida-Cardenas, Hala Abbas, Kerri Hill-Cawthorne, Frances Bolt, Elita Jauneikaite, Frances Davies, Alison Holmes, Pantelis Georgiou, Jesus Rodriguez-Manzano
Summary: The novel methodology of combining machine learning algorithms with real-time digital PCR instruments has shown promising results in improving the identification of carbapenem-resistant genes in clinical CPO-isolates. This approach has demonstrated increased classification accuracy in detecting these genes without hardware modifications and at no additional costs, potentially providing significant clinical utility in screening patients for CPO carriage.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Correction
Environmental Sciences
X. Fernandez-Cassi, N. Timoneda, S. Martinez-Puchol, M. Rusinol, J. Rodriguez-Manzano, N. Figuerola, S. Bofill-Mas, J. F. Abril, R. Girones
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Veterinary Sciences
Oliver W. Stringer, Yanwen Li, Janine T. Bosse, Matthew S. Forrest, Juan Hernandez-Garcia, Alexander W. Tucker, Tiago Nunes, Francisco Costa, Preben Mortensen, Eduardo Velazquez, Paul Penny, Jesus Rodriguez-Manzano, Pantelis Georgiou, Paul R. Langford
Summary: Actinobacillus pleuropneumoniae (APP) is an important veterinary pathogen causing porcine pleuropneumoniae with high economic impact worldwide. Good biosecurity measures including regular diagnostic testing are essential. This study developed an isothermal point-of-care diagnostic test using recombinase polymerase amplification (RPA) and targeting the species-specific apxIVA gene, which achieved rapid and sensitive screening for APP.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Pediatrics
Rowan Kuiper, Victoria J. Wright, Dominic Habgood-Coote, Chisato Shimizu, Daphne Huigh, Adriana H. Tremoulet, Danielle van Keulen, Clive J. Hoggart, Jesus Rodriguez-Manzano, Jethro A. Herberg, Myrsini Kaforou, Dennie Tempel, Jane C. Burns, Michael Levin
Summary: This study successfully translates a microarray-based classifier into a more rapid and cost-efficient qRT-PCR assay, the KiDs-GEP classifier, bringing a diagnostic test for Kawasaki disease closer to the hospital clinical laboratory.
PEDIATRIC RESEARCH
(2023)
Article
Multidisciplinary Sciences
Benjamin W. Wormald, Nicolas Moser, Nandita M. deSouza, Katerina-Theresa Mantikas, Kenny Malpartida-Cardenas, Ivana Pennisi, Thomas E. J. Ind, Katherine Vroobel, Melpomeni Kalofonou, Jesus Rodriguez-Manzano, Pantelis Georgiou
Summary: This study demonstrates the efficacy of a handheld Lab-on-Chip device for detecting cervical cancer from biopsy samples. The device uses ISFET sensors and LAMP assays to amplify and detect HPV DNA and hTERT mRNA. The results show that these markers can be used for cervical cancer detection in tissue samples.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Francesca Romana Cavallo, Khalid Baig Mirza, Sara de Mateo, Luca Miglietta, Jesus Rodriguez-Manzano, Konstantin Nikolic, Christofer Toumazou
Summary: This paper presents a fully automated point-of-care device for protein quantification using short-DNA aptamers. The device integrates DNA amplification, sensing, and real-time data processing, and can be used for disease diagnostics and personalized medicine. Additionally, a new algorithm is proposed for automated and real-time protein quantification. An alternative method is also introduced, which utilizes a convolutional neural network algorithm to classify samples and provide clinically relevant advice.
Review
Chemistry, Analytical
Louis Kreitmann, Luca Miglietta, Ke Xu, Kenny Malpartida-Cardenas, Giselle D'Souza, Myrsini Kaforou, Karen Brengel-Pesce, Laurent Drazek, Alison Holmes, Jesus Rodriguez-Manzano
Summary: qPCR is a powerful tool for accurate nucleic acid detection and quantification, but current methods for multiplexing are limited to a small number of targets or require expensive instrumentation. This review discusses the use of machine learning algorithms and data-driven solutions to enhance multiplexing in qPCR. The review also explores the use of computer simulation to optimize the design of mPCR assays. The integration of knowledge-based and data-driven software solutions could streamline assay design and improve target detection and quantification in the multiplex setting.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Environmental Sciences
Marta Itarte, Silvia Bofill-Mas, Sandra Martinez-Puchol, Helena Torrell, Adria Cereto, Marina Carrasco, Eva Fores, Nuria Canela, Rosina Girones, Marta Rusinol
Summary: Researchers summarized the different analytical approaches currently used to study the dissemination of SARS-CoV-2 variants in wastewater and discussed their advantages and disadvantages, while providing preliminary results of two sensitive and cost-effective research methods. Next-generation sequencing approaches allow for simultaneous detection of signature mutations of different variants of concern, whereas targeted PCR methods require continuous updating but are sensitive and cost-effective.
CURRENT OPINION IN ENVIRONMENTAL SCIENCE & HEALTH
(2021)
