Article
Immunology
Pujun Liang, Mingliu Wang, Marcelo Gottschalk, Ana Vela, April A. Estrada, Jianping Wang, Pengcheng Du, Ming Luo, Han Zheng, Zongfu Wu
Summary: This study confirmed the non-negligible pathogenicity of Streptococcus suis serotype 7 and deepened the understanding of its population structure, providing valuable information for improved surveillance of this serotype.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Immunology
Mei-Fang Tan, Jia Tan, Fan-Fan Zhang, Hai-Qin Li, Hua-Yuan Ji, Shao-Pei Fang, Cheng-Cheng Wu, Yu-Ling Rao, Yan-Bin Zeng, Qun Yang
Summary: This study investigated the pleiotropic effects of exogenous glycogen on Streptococcus suis serotype 2 (SS2) through transcriptome sequencing. The results showed that glycogen induction significantly influenced the metabolic pathways and virulence-associated factors of SS2. Extracellular glycogen utilization enhanced the hemolytic activity, adhesion and invasion ability, and pathogenicity of SS2. These findings are important for understanding the adaptation and pathogenicity mechanisms of SS2.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Quan Li, Xia Fei, Yuhang Zhang, Genglin Guo, Huoying Shi, Wei Zhang
Summary: The study identified a putative virulence-associated factor MutT unique to S. suis serotype 2 virulent strains, essential for the pathogenicity of SS2. MutT knockout mutant significantly decreased bacterial virulence and increased susceptibility to oxidative stress, while enhancing adhesion and invasion to host cells. Deletion of mutT also led to increased microbial clearance in infected host tissues, indicating the crucial biological role of MutT in bacterial pathogenesis during infection.
Article
Multidisciplinary Sciences
Ching-Fen Wu, Siou-Hui Chen, Chi-Chung Chou, Chao-Min Wang, Szu-Wei Huang, Hung-Chih Kuo
Summary: This study comprehensively characterized S. suis isolates from diseased pigs in Taiwan, revealing the prevalence of serotypes 3, 7, and 8, and the presence of novel sequence types and clonal complexes. The isolates showed high susceptibility to multiple antimicrobial agents. The distribution of different serotypes varied in different tissue samples.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Shujie Wang, Min Xu, Kongbin Yang, Ying Zhang, Siqi Li, Yan-Dong Tang, Jinliang Wang, Chaoliang Leng, Tongqing An, Xuehui Cai
Summary: After infection with highly pathogenic PRRSV, there were more severe clinical symptoms, mortality, and inguinal lymph node lesions, and the infection with S. suis exacerbated the apoptosis of immune cells. This study contributes to a better understanding of the pathogenic mechanisms during secondary S. suis infection.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Anna Werinder, Anna Aspan, Magdalena Jacobson, Annette Backhans, Marie Sjolund, Bengt Guss, Robert Soderlund
Summary: The aim of this study was to identify and characterize differences in the genomes between Swedish S. suis isolates associated with disease and isolates from healthy animals. Whole-genome sequencing, serotyping, and sequence-typing were used to analyze the isolates, and a genome-wide association study was performed to identify virulence-associated genes. The results showed that serotype 2, 1, and 7 were the most common in the pathogenic group, while serotype 15 and 12 were the most common in the non-pathogenic group.
VETERINARY MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Anusak Kerdsin, Dan Takeuchi, Yukihiro Akeda, Shota Nakamura, Marcelo Gottschalk, Kazunori Oishi
Summary: This study compared the genomes of ST1 and ST104 strains of Streptococcus suis and identified 58 unique genomic regions in the ST104 strain, primarily related to metabolism, cell functions, cell wall proteins, bacteriophages, transporters, signal transductions, and lantibiotic proteins. Some virulence genes found in ST1 strains were also present in the ST104 genome. Genomic comparison is a powerful tool for understanding the molecular basis of virulence in S. suis infections.
Article
Veterinary Sciences
Servane Payen, Jesus Aranda Rrodriguez, Mariela Segura, Marcelo Gottschalk
Summary: Streptococcus suis serotype 2 is a significant pathogen in swine, causing economic losses to the industry. The role of the Lmb protein in its pathogenesis was examined and found to have no effect on laminin-binding activity or bacterial adhesion and invasion. However, it was shown to activate cytokines in vitro and play a critical role in zinc acquisition from the host environment.
VETERINARY RESEARCH
(2023)
Article
Microbiology
Shujie Wang, Gang Wang, Yan-Dong Tang, Siqi Li, Lei Qin, Menghang Wang, Yong-Bo Yang, Marcelo Gottschalk, Xuehui Cai
Summary: This study reveals the mechanisms underlying splenic lesions caused by Streptococcus suis infection, including apoptosis and activation of inflammatory responses. The findings provide new insights into the pathogenesis of S. suis.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Xiaowu Jiang, Lexin Zhu, Dongbo Zhan
Summary: This study discovered a newly secreted effector protein, LacD, in Streptococcus suis type 2 (SS2) that plays multiple roles in metabolism, stress response, and virulence. Through gene mutant analysis, it was found that the deletion of LacD increased susceptibility to oxidative stress and antibiotics, and weakened invasion capability and virulence. Transcriptomic profiling further confirmed the involvement of LacD in cell division, stress response, antimicrobial susceptibility, and virulence regulation.
JOURNAL OF MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Zhuohao Wang, Yu Zhou, Genglin Guo, Quan Li, Yanfei Yu, Wei Zhang, Martha Vives
Summary: This study reveals a strong correlation between mass spectra pattern and virulence phenotype among Streptococcus suis serotype 2 (SS2) for the first time. Machine learning-based classifiers were established and achieved an accuracy of over 90%. Overall, this study paves the way for rapid detection of virulent SS2 and provides new ideas for genotype-dependent detection of specific pathogens.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Infectious Diseases
Genglin Guo, Xuewei Kong, Dechao Du, Dan Wei, Yanfei Yu, Wei Zhang
Summary: This study assessed the virulence phenotypes of Streptococcus suis using a zebrafish model and identified 172 potential virulence factors, with 143 of them present in virulent isolates. Protein-protein interaction analysis revealed that these genes could interact with known virulence factors and play important roles as bridges or hubs.
INFECTION GENETICS AND EVOLUTION
(2021)
Article
Biology
Huanhuan Yang, Jingjing Huang, Xiaotong Hu, Min Hu, Qiang Zhang, Meilin Jin
Summary: The study investigated the serotypes of Streptococcus suis serotype 9 strains from China and compared them with those from the Netherlands and the U.K. Phylogenetic analysis showed that Chinese and U.K. strains were heterogeneous, while most strains from the Netherlands belonged to the same clade. The distribution of virulence-associated genes and antibiotic resistance genes varied among different clades and sources, suggesting diverse evolutionary paths between China and Europe for S. suis serotype 9.
Article
Veterinary Sciences
Carolin Liedel, Leonie Mayer, Almuth Einspanier, Iris Voelker, Reiner Ulrich, Karoline Rieckmann, Christoph G. Baums
Summary: Experimental studies on a new S. suis cps3 infection model in pigs revealed that buprenorphine treatment did not prevent severe distress, high cortisol levels, and significant pathologies. Serum cortisol levels proved to be a meaningful parameter for assessing distress in experimentally infected piglets.
BMC VETERINARY RESEARCH
(2022)
Article
Infectious Diseases
Maximo Petrocchi-Rilo, Sonia Martinez-Martinez, Alvaro Aguaron-Turrientes, Elisabet Roca-Martinez, Maria-Jose Garcia-Iglesias, Esther Perez-Fernandez, Alba Gonzalez-Fernandez, Elena Herencia-Lagunar, Cesar-Bernardo Gutierrez-Martin
Summary: A diverse set of Streptococcus suis isolates from Spain in 2019 to 2020 showed various serotypes and pathotypes, with serotypes 2 and 9 mainly associated with CNS infections, serotype 1 with joint infections, and serotype 3 with lung infections. The S. suis isolates exhibited high global antimicrobial resistance, with ampicillin, spectinomycin, and tiamulin showing the highest activities.