Article
Biotechnology & Applied Microbiology
Guohui Cui, Lulu Si, Ying Wang, Junmei Zhou, Huijun Yan, Lifang Jiang
Summary: Replacing the pr4 gene of DENV with the JEV gene can reduce ADE activities and virulence of the virus. Leucine, phenylalanine, and cysteine in pr4 are key amino acid sites in the ADE response.
JOURNAL OF GENE MEDICINE
(2021)
Article
Virology
Guohua Li, Xianyong Meng, Zhiguang Ren, Entao Li, Feihu Yan, Jing Liu, Ying Zhang, Zhanding Cui, Yuetao Li, Hongli Jin, Zengguo Cao, Le Yi, Pei Huang, Hang Chi, Hualei Wang, Weiyang Sun, Tiecheng Wang, Yuwei Gao, Yongkun Zhao, Songtao Yang, Xianzhu Xia
Summary: West Nile virus disease is caused by the arthropod-borne West Nile virus, posing a significant public health risk. The constructed ChiVax-WN01 chimera exhibits distinct characteristics compared to other strains, with moderate safety and immunogenicity demonstrated in animal studies.
Article
Multidisciplinary Sciences
Keisuke Maezono, Shintaro Kobayashi, Koshiro Tabata, Kentaro Yoshii, Hiroaki Kariwa
Summary: Deletion of the C-terminal region of E protein suppressed the formation of subviral particles (SPs) without affecting E protein production. The sensitivity of ELISA using full-length E protein was higher than that of truncated E proteins. ELISA using full-length E protein showed little cross-reactivity with anti-JEV antibodies and had similar sensitivity to the neutralization test.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Robert E. Snyder, Gail Sondermeyer Cooksey, Vicki Kramer, Seema Jain, Duc J. Vugia
Summary: WNV-associated hospitalizations in California were substantial and costly, with higher incidence in males, elderly persons, and patients with underlying conditions. WNV remains a costly and severe public health threat in California.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Biochemical Research Methods
Kristina Allgoewer, Shuvadeep Maity, Alice Zhao, Lauren Lashua, Moti Ramgopal, Beni N. Balkaran, Liyun Liu, Savita Purushwani, Maria T. Arevalo, Ted M. Ross, Hyungwon Choi, Elodie Ghedin, Christine Vogel
Summary: Distinguishing between Zika and dengue virus infections is crucial for accurate treatment. Using next-generation proteomics, the study identified 13 differentially expressed proteins related to infection, wound healing, pregnancy, and brain function. Three of these proteins showed potential for predicting Zika virus infection, and temporal changes in protein signatures can help differentiate diagnoses and indicate past infections.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Letter
Immunology
Gino Arcilla, Antoine Nguyen, Antonio Liu
Summary: We encountered a patient with HIV who showed rapid deterioration in mental status and was positive for both acute West Nile encephalitis and amphiphysin antibodies. After reviewing the literature, we found Dr. Moutsopoulos's paper from your journal highly interesting (Karagianni et al., 2019 [1]). Although autoimmune encephalitis following West Nile encephalitis is not uncommon, there are several intriguing aspects in our patient's case. Firstly, the coexistence of amphiphysin antibodies with West Nile encephalitis has not been reported before. Secondly, the monophasic clinical course suggests that autoimmune encephalitis triggered by or coexisting with West Nile encephalitis may be significantly underrecognized. Lastly, our patient was HIV positive but not severely immunocompromised, which may have contributed to the autoimmune status.
CLINICAL IMMUNOLOGY
(2023)
Article
Microbiology
Zhancheng Tian, Hongge Zhang, Ruiming Yu, Junzheng Du, Shandian Gao, Qiongjie Wang, Guiquan Guan, Hong Yin
Summary: The levels of swine GBP1 (sGBP1) were increased at the late stage of Japanese encephalitis virus (JEV) infection. Overexpression of sGBP1 inhibited JEV production, while knockdown of sGBP1 promoted JEV production. The GTPase activity and isoprenylation of sGBP1 were both critical for its anti-JEV activity.
VETERINARY MICROBIOLOGY
(2023)
Article
Cell Biology
Sundy N. Y. Yang, Belinda Maher, Chunxiao Wang, Kylie M. Wagstaff, Johanna E. Fraser, David A. Jans
Summary: This study identifies two drugs that can inhibit the replication of dengue virus and other flaviviruses by directly binding to a viral protein.
Article
Virology
Tao Chen, Shuo Zhu, Ning Wei, Zikai Zhao, Junjun Niu, Youhui Si, Shengbo Cao, Jing Ye
Summary: This study developed a modified mRNA vaccine for Japanese encephalitis virus (JEV) and demonstrated its efficacy in a mouse model. The vaccine induced strong immune responses and protected mice from JEV infection. This provides a new option for JE vaccination and lays the foundation for future development of more efficient and safer JEV mRNA vaccines.
Article
Immunology
Yoshua Colyn Collins-Sawaragi, Patra Koletsi, Eleanor Elizabeth Donlevy, Simon Bruce Drysdale
Summary: This article presents a case of initial seronegative West Nile virus encephalitis in an immunocompromised child and highlights that diagnostic guidelines may not be applicable to immunocompromised patients.
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2022)
Article
Immunology
Tomohiro Kotaki, Yurie Nagai, Atsushi Yamanaka, Eiji Konishi, Masanori Kameoka
Summary: This study focused on developing a Japanese encephalitis (JE) vaccine that does not induce antibody-dependent enhancement (ADE) of dengue virus (DENV) infection. By introducing a mutation at the 106th amino acid residue of the JEV E protein, the modified JE vaccine was able to significantly reduce the production of cross-reactive ADE-prone antibodies against DENV.
Review
Pharmacology & Pharmacy
Ivo C. C. Martins, Rafaela C. C. Ricardo, Nuno C. C. Santos
Summary: Dengue, West Nile, and Zika viruses are vector-borne flaviviruses that cause disease outbreaks with potentially severe symptoms and socioeconomic repercussions. Currently, there are no specific treatments available, and only generic symptom relief is possible. The effectiveness of the first dengue vaccine, Dengvaxia, is limited. Prophylactic approaches against other viruses are even more restricted. Therefore, there is a need for therapeutic strategies.
Article
Virology
Poonsook Keelapang, Piyada Supasa, Rungtawan Sriburi, Chunya Puttikhunt, Jane Cardosa, Watchara Kasinrerk, Prida Malasit, Nopporn Sittisombut
Summary: Partial cleavage of prM protein in dengue virus leads to a mixture of extracellular particles with varying levels of maturation and infectivity. Antibody-mediated infection involves prM epitopes localized to the pr domain. A group of murine anti-prM monoclonal antibodies were found to reduce the focus size of subsets of dengue serotypes. Further mapping experiments revealed that the major binding site of these antibodies is located in the 'a' and 'c' strands region of pr domain.
Article
Virology
Stefanie Ganzenberg, Michael Sieg, Ute Ziegler, Martin Pfeffer, Thomas W. Vahlenkamp, Uwe Hoeruegel, Martin H. Groschup, Katharina L. Lohmann
Summary: This study determined the seroprevalence of West Nile virus (WNV) in horses in nine counties in Eastern Germany and estimated the risk factors for seropositivity. The results showed that the seroprevalence of WNV infection was significantly higher in counties with previously registered equine WNV infections. Breed type (pony), housing in counties with previously registered infections, housing type (24 h turn-out), and presence of outdoor shelter were identified as the main significant risk factors for seropositivity.
Article
Virology
Phebe de Heus, Jolanta Kolodziejek, Zdenek Hubalek, Katharina Dimmel, Victoria Racher, Norbert Nowotny, Jessika-M Cavalleri
Summary: The study found neutralizing antibody prevalence rates for WNV and TBEV in horses in eastern Austria to be 5.3% and 15.5% respectively, with USUV showing 0% prevalence and 1.2% for autochthonous WNV. Furthermore, no significant risk factors for seropositivity were identified.
Article
Biochemistry & Molecular Biology
Chao Zhou, Qi Chen, Yun Chen, Cheng-Feng Qin
Summary: This review summarizes the current status of oncolytic viruses for GBM treatment, with a focus on the potential of Zika virus as a therapeutic virus for GBM and the associated advantages and challenges for its clinical development.
DNA AND CELL BIOLOGY
(2023)
Article
Virology
Nina Sun, Rong-Rong Zhang, Guang-Yuan Song, Qiaomei Cai, Saba R. R. Aliyari, Karin Nielsen-Saines, Jae U. U. Jung, Heng Yang, Genhong Cheng, Cheng-Feng Qin
Summary: SERTAD3 interacts with the capsid protein of Zika virus and decreases its abundance through proteasomal degradation, thereby providing an antiviral defense against Zika virus infection. The study elucidates the mechanism of action of SERTAD3 and suggests it as a potential therapeutic target for Zika virus infection.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Virology
Yi-Fei Zhang, Jing-Jing Guo, Fan Yang, Hang-Yu Zhou, Na-Na Zhang, Xiao-Chuan Xiong, Yue Feng, Yong-Qiang Deng, Cheng-Feng Qin
Summary: This study characterized a new Zika virus (ZIKV) isolate obtained from Cambodia in 2019 and compared it with a Cambodian strain from 2010. The 2019 isolate showed similar in vitro and in vivo properties compared to the 2010 strain, but exhibited enhanced neurovirulence in neonatal mice and produced higher viral loads in the brains. Sequence alignment revealed 12 amino acid substitutions in the 2019 isolate, some of which were associated with neurovirulence.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Chemistry, Multidisciplinary
Ningke Hou, Lei Shuai, Lijing Zhang, Xuping Xie, Kaiming Tang, Yunkai Zhu, Yin Yu, Wenyi Zhang, Qiaozhu Tan, Gongxun Zhong, Zhiyuan Wen, Chong Wang, Xijun He, Hong Huo, Haishan Gao, You Xu, Jing Xue, Chen Peng, Jing Zou, Craig Schindewolf, Vineet Menachery, Wenji Su, Youlang Yuan, Zuyuan Shen, Rong Zhang, Shuofeng Yuan, Hongtao Yu, Pei-Yong Shi, Zhigao Bu, Jing Huang, Qi Hu
Summary: A specific noncovalent inhibitor called WU-04 has been developed, which effectively blocks the replication of SARS-CoV-2 and other coronaviruses. It shows high potency as a pan-inhibitor of coronavirus 3CLpro and has similar activity to the drug Nirmatrelvir in treating SARS-CoV-2.
ACS CENTRAL SCIENCE
(2023)
Article
Virology
Xinxin Wang, Zhiyuan Wen, Huizhen Cao, Jie Luo, Lei Shuai, Chong Wang, Jinying Ge, Xijun Wang, Zhigao Bu, Jinliang Wang
Summary: For most viruses, cell entry involves engagement with many distinct plasma membrane components, each of which is essential. After binding to its specific receptor(s), rabies virus (RABV) enters host cells through the process of clathrin-mediated endocytosis. Transferrin receptor 1 (TfR1) is identified as an entry factor for RABV infection, and the endocytosis of RABV is coupled with the endocytosis of TfR1. Our results indicate that RABV hijacks the transport pathway of TfR1 for entry, deepening our understanding of the entry mechanism of RABV.
JOURNAL OF VIROLOGY
(2023)
Article
Pediatrics
Ri De, Yan-Peng Xu, Fang Wang, Yu-Tong Zhou, Pan-Deng Shi, Ru-Nan Zhu, Yu Sun, Li-Ying Liu, Li-Ping Jia, Hui-Jin Dong, Hui Zhao, Cheng-Feng Qin, Lin-Qing Zhao
Summary: Through studying the divergent regions (DRs) on the major capsid protein VP3 of HBoV1 and HBoV2, it was found that DR2 had genotype specificity, reacting only with HBoV1. This study is important for accurate detection of HBoV1 and HBoV2 infections.
WORLD JOURNAL OF PEDIATRICS
(2023)
Article
Multidisciplinary Sciences
Xiang Chen, Yan Wang, Zhonghe Xu, Meng-Li Cheng, Qing-Qing Ma, Rui-Ting Li, Zheng-Jian Wang, Hui Zhao, Xiaobing Zuo, Xiao-Feng Li, Xianyang Fang, Cheng-Feng Qin
Summary: This study characterizes the interactions between human RNA binding protein Musashi-1 and Zika virus RNA in neural progenitor cells, shedding light on its role in viral neurotropism.
NATURE COMMUNICATIONS
(2023)
Article
Immunology
Rong-Rong Zhang, Zheng-Jian Wang, Yi-Long Zhu, Wei Tang, Chao Zhou, Suo-Qun Zhao, Mei Wu, Tao Ming, Yong-Qiang Deng, Qi Chen, Ning-Yi Jin, Qing Ye, Xiao Li, Cheng-Feng Qin
Summary: The re-emerging mpox virus has become a public health emergency of international concern and developing a safe and effective mRNA vaccine against it is a top priority. Using a lipid nanoparticle-encapsulated mRNA vaccine platform, a panel of multicomponent MPXV vaccine candidates were constructed and prepared, which elicited strong immune responses in mice. Particularly, the penta- and tetra-component vaccine candidates showed superior capability of inducing neutralizing antibodies and protecting mice from VACV challenge. This study provides critical insights for understanding the protection mechanism of MPXV infection and supports further clinical development of these multicomponent mRNA vaccine candidates.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Medicine, General & Internal
Jia Zhou, Meng-Yue Guan, Rui-Ting Li, Yi-Ni Qi, Guan Yang, Yong-Qiang Deng, Xiao-Feng Li, Liang Li, Xiao Yang, Jian-Feng Liu, Cheng-Feng Qin
Summary: This study investigates the susceptibility and biological responses of the olfactory system to Zika virus (ZIKV) infection. The results show that ZIKV can invade the olfactory system and lead to post-viral olfactory dysfunction (PVOD). ZIKV mainly targets olfactory ensheathing cells (OECs) and exhibits broad cellular tropism in the olfactory mucosa (OM) of immunodeficient mice.
Article
Multidisciplinary Sciences
Hao Wu, Xing-Yao Huang, Meng-Xu Sun, Yue Wang, Hang-Yu Zhou, Ying Tian, Beijia He, Kai Li, De-Yu Li, Ai-Ping Wu, Hongmei Wang, Cheng-Feng Qin
Summary: This study investigated the infectivity and pathological effects of Zika virus (ZIKV) on placental trophoblast progenitor cells in early human embryos. The researchers found that human trophoblast stem cells (hTSCs) were susceptible to ZIKV infection, but resistance increased with cell differentiation. ZIKV infection disrupted the structure of mature hTSC-organoids and inhibited syncytialization. The researchers also suggested that ZIKV infection may lead to a preeclampsia phenotype.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Guang-Yuan Song, Xing-Yao Huang, Meng-Jiao He, Hang-Yu Zhou, Rui-Ting Li, Ying Tian, Yan Wang, Meng-Li Cheng, Xiang Chen, Rong-Rong Zhang, Chao Zhou, Jia Zhou, Xian-Yang Fang, Xiao-Feng Li, Cheng-Feng Qin
Summary: The study identified a specific amino acid substitution, K101R, in the capsid protein of the African lineage Zika virus, which contributes to enhanced replication and inflammatory responses, thus increasing its virulence phenotype compared to the Asian lineage.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Yali Ci, Kai Han, Jie Kong, Shuhan Huang, Yang Yang, Cheng-Feng Qin, Lei Shi
Summary: This study reveals that Flavivirus remodels the host endoplasmic reticulum (ER) to generate replication compartments (RCs) and adopts a centralized replication mode. Superresolution live-cell imaging demonstrates the formation of perinuclear main replication compartments (MRCs) through a series of events, including multisite ER clustering, growth and merging of ER clusters, directional movement, and convergence in the perinuclear region. These dynamic activities are driven by nonstructural (NS) proteins and are independent of microtubules and actin. Disrupting the formation of MRCs inhibits flavivirus replication.
Article
Chemistry, Multidisciplinary
Pan-Deng Shi, Yan-Peng Xu, Zhu Zhu, Chao Zhou, Mei Wu, Yangzhige He, Hui Zhao, Liying Liu, Linqing Zhao, Xiao-Feng Li, Cheng-Feng Qin
Summary: Biomimetic viral mineralization of manganese in viruses provides a rapid and universal strategy for generating emergency vaccines to respond to emerging viruses. Manganese-mineralized viruses induce robust immune responses and offer significant protection against wild-type viruses through a single-dose vaccination.
Article
Virology
Dan Luo, Qing Ye, Rui-Ting Li, Hang-Yu Zhou, Jing-Jing Guo, Suo-Qun Zhao, Sen Zhang, Tao Jiang, Yong-Qiang Deng, Cheng-Feng Qin
Summary: ZX-7101A, an analogue of BXM, shows broad-spectrum antiviral activity against different subtypes of influenza A virus. In mice, ZX-7101A can effectively protect against lethal pH1N1 infection. Furthermore, a single amino acid substitution (E18G) is identified to be responsible for the resistance to both ZX-7101A and BXM.
Article
Multidisciplinary Sciences
Suoqun Zhao, Hang Chi, Chengfeng Qin, Yongqiang Deng
Summary: Emerging viral infectious diseases are diverse, fast-spreading and dangerous, causing outbreaks and epidemics worldwide with serious effects on health, society and economy. Vaccines, small-molecule drugs and monoclonal antibodies are important for addressing these diseases, particularly the monoclonal antibodies, which can effectively prevent the virus from entering host cells, block reinfection, and aid in viral clearance.
CHINESE SCIENCE BULLETIN-CHINESE
(2023)